A Measurement Method for Cytochrome P450 3A4 (CYP3A4)-mediated Oxidation of Cholesterol in Lipid Membranes

We have developed a measurement method for the cytochrome P450 3A4 (CYP3A4)-mediated oxidation of cholesterol in lipid membranes. This method is based on the sterol-structural selectivity for the nanopore formation of streptolysin O (SLO) in a lipid membrane. A decrease in the fraction of 4β-hydroxy...

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Veröffentlicht in:	Sensors and materials 2022-01, Vol.34 (3), p.951
Hauptverfasser:	Shoji, Atsushi, Miki, Haruka, Kikkawa, Mikiko, Yamamoto, Miyu, Moriiwa, Yukiko, Morioka, Kazuhiro, Yanagida, Akio
Format:	Artikel
Sprache:	eng
Schlagworte:	Cholesterol Cytochrome Cytochromes P450 Erythromycin Lipids Measurement methods Membranes Oxidation Phosphatidylcholine Selectivity
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creator	Shoji, Atsushi Miki, Haruka Kikkawa, Mikiko Yamamoto, Miyu Moriiwa, Yukiko Morioka, Kazuhiro Yanagida, Akio
description	We have developed a measurement method for the cytochrome P450 3A4 (CYP3A4)-mediated oxidation of cholesterol in lipid membranes. This method is based on the sterol-structural selectivity for the nanopore formation of streptolysin O (SLO) in a lipid membrane. A decrease in the fraction of 4β-hydroxycholesterol in lipid membranes decreased the nanopore forming ability of SLO. The incubation of the liposomes consisting of l-α-phosphatidylcholine (PC), l-α-phosphatidylethanolamine (PE), and cholesterol (0.98:0.02:0.75, mol/mol) with CYP3A4 decreased the nanopore forming ability of SLO, indicating the conversion of cholesterol to 4β-hydroxycholesterol. The change in nanopore forming ability did not occur in the presence of erythromycin, which is an inhibitor of CYP3A4.
doi_str_mv	10.18494/SAM3598
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subjects	Cholesterol Cytochrome Cytochromes P450 Erythromycin Lipids Measurement methods Membranes Oxidation Phosphatidylcholine Selectivity
title	A Measurement Method for Cytochrome P450 3A4 (CYP3A4)-mediated Oxidation of Cholesterol in Lipid Membranes
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