Long-term chronic caloric restriction alters miRNA profiles in the brain of ageing mice
Calorie restriction (CR) has been shown to be one of the most effective methods in alleviating the effects of ageing and age-related diseases. Although the protective effects of CR have been reported, the exact molecular mechanism still needs to be clarified. This study aims to determine differentia...
Gespeichert in:
| Veröffentlicht in: | British journal of nutrition 2022-03, Vol.127 (5), p.641-652 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 652 |
|---|---|
| container_issue | 5 |
| container_start_page | 641 |
| container_title | British journal of nutrition |
| container_volume | 127 |
| creator | Ozorhan, Umit Tuna, Bilge G. Cicekdal, Munevver B. Kuskucu, Aysegul Bayrak, Omer F. Yilmaz, Bayram Demirel, Pinar B. Cleary, Margot P. Dogan, Soner |
| description | Calorie restriction (CR) has been shown to be one of the most effective methods in alleviating the effects of ageing and age-related diseases. Although the protective effects of CR have been reported, the exact molecular mechanism still needs to be clarified. This study aims to determine differentially expressed (DE) miRNAs and altered gene pathways due to long-term chronic (CCR) and intermittent (ICR) CR in the brain of mice to understand the preventive roles of miRNAs resulting from long-term CR. Ten weeks old mice were enrolled into three different dietary groups; ad libitum, CCR or ICR, and fed until 82 weeks of age. miRNAs were analysed using GeneChip 4.1 microarray and the target of DE miRNAs was determined using miRNA target databases. Out of a total 3,163 analysed miRNAs, 55 of them were differentially expressed either by different CR protocols or by ageing. Brain samples from the CCR group had increased expression levels of mmu-miR-713 while decreasing expression levels of mmu-miR-184-3p and mmu-miR-351-5p compared to the other dietary groups. Also, current results indicated that CCR showed better preventive effects than that of ICR. Thus, CCR may perform its protective effects by modulating these specific miRNAs since they are shown to play roles in neurogenesis, chromatin and histone regulation. In conclusion, these three miRNAs could be potential targets for neurodegenerative and ageing-related diseases and may play important roles in the protective effects of CR in the brain. |
| doi_str_mv | 10.1017/S0007114521001239 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2632938161</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cupid>10_1017_S0007114521001239</cupid><sourcerecordid>2632938161</sourcerecordid><originalsourceid>FETCH-LOGICAL-c416t-f026637e961c94e1c954b8afa5a4cb3ac01941b4a2ba025d1a6a45997e98ad7a3</originalsourceid><addsrcrecordid>eNp1UMtOwzAQtBCIlsIHcEGWOAe8tvPwsap4SRVIPMQx2jhO6qqJi50e-HtctcABcfF4tTOzoyHkHNgVMMivXxhjOYBMOTAGXKgDMgaZpwnPMn5Ixtt1st2PyEkIyzgWwNQxGQlRRLbMx-R97vo2GYzvqF5411tNNa6cj-hNGCIO1vUUV5ESaGefH6d07V1jVyZQ29NhYWjlMf5cQ7E1tm8jS5tTctTgKpizPU7I2-3N6-w-mT_dPcym80RLyIakYTGqyI3KQCtp4pPKqsAGU5S6EqgZKAmVRF4h42kNmKFMlYqKAuscxYRc7nxjqI9NTFwu3cb38WTJM8GVKCCDyIIdS3sXgjdNufa2Q_9ZAiu3VZZ_qoyai73zpupM_aP47i4SxN4Uu8rbujW_t_-3_QKLvXzJ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2632938161</pqid></control><display><type>article</type><title>Long-term chronic caloric restriction alters miRNA profiles in the brain of ageing mice</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><source>Cambridge University Press Journals Complete</source><creator>Ozorhan, Umit ; Tuna, Bilge G. ; Cicekdal, Munevver B. ; Kuskucu, Aysegul ; Bayrak, Omer F. ; Yilmaz, Bayram ; Demirel, Pinar B. ; Cleary, Margot P. ; Dogan, Soner</creator><creatorcontrib>Ozorhan, Umit ; Tuna, Bilge G. ; Cicekdal, Munevver B. ; Kuskucu, Aysegul ; Bayrak, Omer F. ; Yilmaz, Bayram ; Demirel, Pinar B. ; Cleary, Margot P. ; Dogan, Soner</creatorcontrib><description>Calorie restriction (CR) has been shown to be one of the most effective methods in alleviating the effects of ageing and age-related diseases. Although the protective effects of CR have been reported, the exact molecular mechanism still needs to be clarified. This study aims to determine differentially expressed (DE) miRNAs and altered gene pathways due to long-term chronic (CCR) and intermittent (ICR) CR in the brain of mice to understand the preventive roles of miRNAs resulting from long-term CR. Ten weeks old mice were enrolled into three different dietary groups; ad libitum, CCR or ICR, and fed until 82 weeks of age. miRNAs were analysed using GeneChip 4.1 microarray and the target of DE miRNAs was determined using miRNA target databases. Out of a total 3,163 analysed miRNAs, 55 of them were differentially expressed either by different CR protocols or by ageing. Brain samples from the CCR group had increased expression levels of mmu-miR-713 while decreasing expression levels of mmu-miR-184-3p and mmu-miR-351-5p compared to the other dietary groups. Also, current results indicated that CCR showed better preventive effects than that of ICR. Thus, CCR may perform its protective effects by modulating these specific miRNAs since they are shown to play roles in neurogenesis, chromatin and histone regulation. In conclusion, these three miRNAs could be potential targets for neurodegenerative and ageing-related diseases and may play important roles in the protective effects of CR in the brain.</description><identifier>ISSN: 0007-1145</identifier><identifier>EISSN: 1475-2662</identifier><identifier>DOI: 10.1017/S0007114521001239</identifier><identifier>PMID: 33823947</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Age ; Age related diseases ; Aging ; Aging - physiology ; Animal cognition ; Animals ; Brain ; Brain - metabolism ; Brain research ; Caloric Restriction - methods ; Chromatin ; Chronic illnesses ; Cognitive ability ; Dietary restrictions ; DNA methylation ; DNA microarrays ; Food ; Gene expression ; Growth factors ; Histones ; Mice ; Mice, Inbred ICR ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Molecular Nutrition ; Neurodegeneration ; Neurogenesis ; Nutrient deficiency ; Ontology ; Physiology ; Traumatic brain injury</subject><ispartof>British journal of nutrition, 2022-03, Vol.127 (5), p.641-652</ispartof><rights>The Author(s), 2021. Published by Cambridge University Press on behalf of The Nutrition Society</rights><rights>The Author(s), 2021. Published by Cambridge University Press on behalf of The Nutrition Society. This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike licence (http://creativecommons.org/licenses/by-nc-sa/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the same Creative Commons licence is included and the original work is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the associated terms available at: https://uk.sagepub.com/en-gb/eur/reusing-open-access-and-sage-choice-content</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-f026637e961c94e1c954b8afa5a4cb3ac01941b4a2ba025d1a6a45997e98ad7a3</citedby><cites>FETCH-LOGICAL-c416t-f026637e961c94e1c954b8afa5a4cb3ac01941b4a2ba025d1a6a45997e98ad7a3</cites><orcidid>0000-0002-7661-2818 ; 0000-0002-1986-0815 ; 0000-0003-1348-1336</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0007114521001239/type/journal_article$$EHTML$$P50$$Gcambridge$$Hfree_for_read</linktohtml><link.rule.ids>164,315,781,785,27926,27927,55630</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33823947$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ozorhan, Umit</creatorcontrib><creatorcontrib>Tuna, Bilge G.</creatorcontrib><creatorcontrib>Cicekdal, Munevver B.</creatorcontrib><creatorcontrib>Kuskucu, Aysegul</creatorcontrib><creatorcontrib>Bayrak, Omer F.</creatorcontrib><creatorcontrib>Yilmaz, Bayram</creatorcontrib><creatorcontrib>Demirel, Pinar B.</creatorcontrib><creatorcontrib>Cleary, Margot P.</creatorcontrib><creatorcontrib>Dogan, Soner</creatorcontrib><title>Long-term chronic caloric restriction alters miRNA profiles in the brain of ageing mice</title><title>British journal of nutrition</title><addtitle>Br J Nutr</addtitle><description>Calorie restriction (CR) has been shown to be one of the most effective methods in alleviating the effects of ageing and age-related diseases. Although the protective effects of CR have been reported, the exact molecular mechanism still needs to be clarified. This study aims to determine differentially expressed (DE) miRNAs and altered gene pathways due to long-term chronic (CCR) and intermittent (ICR) CR in the brain of mice to understand the preventive roles of miRNAs resulting from long-term CR. Ten weeks old mice were enrolled into three different dietary groups; ad libitum, CCR or ICR, and fed until 82 weeks of age. miRNAs were analysed using GeneChip 4.1 microarray and the target of DE miRNAs was determined using miRNA target databases. Out of a total 3,163 analysed miRNAs, 55 of them were differentially expressed either by different CR protocols or by ageing. Brain samples from the CCR group had increased expression levels of mmu-miR-713 while decreasing expression levels of mmu-miR-184-3p and mmu-miR-351-5p compared to the other dietary groups. Also, current results indicated that CCR showed better preventive effects than that of ICR. Thus, CCR may perform its protective effects by modulating these specific miRNAs since they are shown to play roles in neurogenesis, chromatin and histone regulation. In conclusion, these three miRNAs could be potential targets for neurodegenerative and ageing-related diseases and may play important roles in the protective effects of CR in the brain.</description><subject>Age</subject><subject>Age related diseases</subject><subject>Aging</subject><subject>Aging - physiology</subject><subject>Animal cognition</subject><subject>Animals</subject><subject>Brain</subject><subject>Brain - metabolism</subject><subject>Brain research</subject><subject>Caloric Restriction - methods</subject><subject>Chromatin</subject><subject>Chronic illnesses</subject><subject>Cognitive ability</subject><subject>Dietary restrictions</subject><subject>DNA methylation</subject><subject>DNA microarrays</subject><subject>Food</subject><subject>Gene expression</subject><subject>Growth factors</subject><subject>Histones</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Molecular Nutrition</subject><subject>Neurodegeneration</subject><subject>Neurogenesis</subject><subject>Nutrient deficiency</subject><subject>Ontology</subject><subject>Physiology</subject><subject>Traumatic brain injury</subject><issn>0007-1145</issn><issn>1475-2662</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>IKXGN</sourceid><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1UMtOwzAQtBCIlsIHcEGWOAe8tvPwsap4SRVIPMQx2jhO6qqJi50e-HtctcABcfF4tTOzoyHkHNgVMMivXxhjOYBMOTAGXKgDMgaZpwnPMn5Ixtt1st2PyEkIyzgWwNQxGQlRRLbMx-R97vo2GYzvqF5411tNNa6cj-hNGCIO1vUUV5ESaGefH6d07V1jVyZQ29NhYWjlMf5cQ7E1tm8jS5tTctTgKpizPU7I2-3N6-w-mT_dPcym80RLyIakYTGqyI3KQCtp4pPKqsAGU5S6EqgZKAmVRF4h42kNmKFMlYqKAuscxYRc7nxjqI9NTFwu3cb38WTJM8GVKCCDyIIdS3sXgjdNufa2Q_9ZAiu3VZZ_qoyai73zpupM_aP47i4SxN4Uu8rbujW_t_-3_QKLvXzJ</recordid><startdate>20220314</startdate><enddate>20220314</enddate><creator>Ozorhan, Umit</creator><creator>Tuna, Bilge G.</creator><creator>Cicekdal, Munevver B.</creator><creator>Kuskucu, Aysegul</creator><creator>Bayrak, Omer F.</creator><creator>Yilmaz, Bayram</creator><creator>Demirel, Pinar B.</creator><creator>Cleary, Margot P.</creator><creator>Dogan, Soner</creator><general>Cambridge University Press</general><scope>IKXGN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7T5</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><orcidid>https://orcid.org/0000-0002-7661-2818</orcidid><orcidid>https://orcid.org/0000-0002-1986-0815</orcidid><orcidid>https://orcid.org/0000-0003-1348-1336</orcidid></search><sort><creationdate>20220314</creationdate><title>Long-term chronic caloric restriction alters miRNA profiles in the brain of ageing mice</title><author>Ozorhan, Umit ; Tuna, Bilge G. ; Cicekdal, Munevver B. ; Kuskucu, Aysegul ; Bayrak, Omer F. ; Yilmaz, Bayram ; Demirel, Pinar B. ; Cleary, Margot P. ; Dogan, Soner</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-f026637e961c94e1c954b8afa5a4cb3ac01941b4a2ba025d1a6a45997e98ad7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Age</topic><topic>Age related diseases</topic><topic>Aging</topic><topic>Aging - physiology</topic><topic>Animal cognition</topic><topic>Animals</topic><topic>Brain</topic><topic>Brain - metabolism</topic><topic>Brain research</topic><topic>Caloric Restriction - methods</topic><topic>Chromatin</topic><topic>Chronic illnesses</topic><topic>Cognitive ability</topic><topic>Dietary restrictions</topic><topic>DNA methylation</topic><topic>DNA microarrays</topic><topic>Food</topic><topic>Gene expression</topic><topic>Growth factors</topic><topic>Histones</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Molecular Nutrition</topic><topic>Neurodegeneration</topic><topic>Neurogenesis</topic><topic>Nutrient deficiency</topic><topic>Ontology</topic><topic>Physiology</topic><topic>Traumatic brain injury</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ozorhan, Umit</creatorcontrib><creatorcontrib>Tuna, Bilge G.</creatorcontrib><creatorcontrib>Cicekdal, Munevver B.</creatorcontrib><creatorcontrib>Kuskucu, Aysegul</creatorcontrib><creatorcontrib>Bayrak, Omer F.</creatorcontrib><creatorcontrib>Yilmaz, Bayram</creatorcontrib><creatorcontrib>Demirel, Pinar B.</creatorcontrib><creatorcontrib>Cleary, Margot P.</creatorcontrib><creatorcontrib>Dogan, Soner</creatorcontrib><collection>Cambridge Journals Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>British journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ozorhan, Umit</au><au>Tuna, Bilge G.</au><au>Cicekdal, Munevver B.</au><au>Kuskucu, Aysegul</au><au>Bayrak, Omer F.</au><au>Yilmaz, Bayram</au><au>Demirel, Pinar B.</au><au>Cleary, Margot P.</au><au>Dogan, Soner</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long-term chronic caloric restriction alters miRNA profiles in the brain of ageing mice</atitle><jtitle>British journal of nutrition</jtitle><addtitle>Br J Nutr</addtitle><date>2022-03-14</date><risdate>2022</risdate><volume>127</volume><issue>5</issue><spage>641</spage><epage>652</epage><pages>641-652</pages><issn>0007-1145</issn><eissn>1475-2662</eissn><abstract>Calorie restriction (CR) has been shown to be one of the most effective methods in alleviating the effects of ageing and age-related diseases. Although the protective effects of CR have been reported, the exact molecular mechanism still needs to be clarified. This study aims to determine differentially expressed (DE) miRNAs and altered gene pathways due to long-term chronic (CCR) and intermittent (ICR) CR in the brain of mice to understand the preventive roles of miRNAs resulting from long-term CR. Ten weeks old mice were enrolled into three different dietary groups; ad libitum, CCR or ICR, and fed until 82 weeks of age. miRNAs were analysed using GeneChip 4.1 microarray and the target of DE miRNAs was determined using miRNA target databases. Out of a total 3,163 analysed miRNAs, 55 of them were differentially expressed either by different CR protocols or by ageing. Brain samples from the CCR group had increased expression levels of mmu-miR-713 while decreasing expression levels of mmu-miR-184-3p and mmu-miR-351-5p compared to the other dietary groups. Also, current results indicated that CCR showed better preventive effects than that of ICR. Thus, CCR may perform its protective effects by modulating these specific miRNAs since they are shown to play roles in neurogenesis, chromatin and histone regulation. In conclusion, these three miRNAs could be potential targets for neurodegenerative and ageing-related diseases and may play important roles in the protective effects of CR in the brain.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>33823947</pmid><doi>10.1017/S0007114521001239</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-7661-2818</orcidid><orcidid>https://orcid.org/0000-0002-1986-0815</orcidid><orcidid>https://orcid.org/0000-0003-1348-1336</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-1145 |
| ispartof | British journal of nutrition, 2022-03, Vol.127 (5), p.641-652 |
| issn | 0007-1145 1475-2662 |
| language | eng |
| recordid | cdi_proquest_journals_2632938161 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry; Cambridge University Press Journals Complete |
| subjects | Age Age related diseases Aging Aging - physiology Animal cognition Animals Brain Brain - metabolism Brain research Caloric Restriction - methods Chromatin Chronic illnesses Cognitive ability Dietary restrictions DNA methylation DNA microarrays Food Gene expression Growth factors Histones Mice Mice, Inbred ICR MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Molecular Nutrition Neurodegeneration Neurogenesis Nutrient deficiency Ontology Physiology Traumatic brain injury |
| title | Long-term chronic caloric restriction alters miRNA profiles in the brain of ageing mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T07%3A57%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Long-term%20chronic%20caloric%20restriction%20alters%20miRNA%20profiles%20in%20the%20brain%20of%20ageing%20mice&rft.jtitle=British%20journal%20of%20nutrition&rft.au=Ozorhan,%20Umit&rft.date=2022-03-14&rft.volume=127&rft.issue=5&rft.spage=641&rft.epage=652&rft.pages=641-652&rft.issn=0007-1145&rft.eissn=1475-2662&rft_id=info:doi/10.1017/S0007114521001239&rft_dat=%3Cproquest_cross%3E2632938161%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2632938161&rft_id=info:pmid/33823947&rft_cupid=10_1017_S0007114521001239&rfr_iscdi=true |