Oxidase Mimic Graphdiyne for Efficient Superoxide Generation in Wide pH Ranges

Great effort has been made in exploring techniques and catalysts for O2•− production but they always work efficiently in either acidic or base environment. Presented here is the design of a unique oxidase mimic catalyst comprising self‐assembled hemin molecules on graphdiyne (hemin/GDY) for efficient O2•− generation in wide pH ranges. Hemin molecules anchor uniformly across the carbon frame of GDY to enable atomic distribution of the active metal sites, delivering a O2•− production rate of 35.7 and 2.3 times than that of hemin and hemin/graphene, respectively. In particular, hemin/GDY can efficiently produce O2•− in acidic, alkaline, and neutral environments, delivering a wide pH behavior. Its oxidase mimic ability is demonstrated for sensing glutathione molecules in cell assays, exhibiting high selectivity, long linear range. This work opens a promising design strategy toward efficient oxidase mimic catalysts for various applications. The oxidase mimic of hemin/graphdiyne (GDY) can produce O2•− with high intensity in acidic, alkaline, and neutral environments, and the strength of O2•− increases with the increase in pH value. The material can realize high‐performance glutathione sensing in cell lysate.