Development of a bio-chemical route to C5 plasticizer synthesis using glutaric acid produced by metabolically engineered Corynebacterium glutamicum
Corynebacterium glutamicum was engineered to produce glutaric acid by metabolic engineering approaches starting from the heterologous introduction of the glutaric acid biosynthesis pathway by the expression of Pseudomonas putida davTDBA genes. Two conversion modules i.e. , l -lysine conversion modul...
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| Veröffentlicht in: | Green chemistry : an international journal and green chemistry resource : GC 2022-02, Vol.24 (4), p.1590-1602 |
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| creator | Sohn, Yu Jung Kang, Minsoo Ryu, Mi-Hee Lee, Siseon Kang, Kyoung Hee Hong, Yunjae Song, Bong Keun Park, Kyungmoon Park, Si Jae Joo, Jeong Chan Kim, Hee Taek |
| description | Corynebacterium glutamicum
was engineered to produce glutaric acid by metabolic engineering approaches starting from the heterologous introduction of the glutaric acid biosynthesis pathway by the expression of
Pseudomonas putida davTDBA
genes. Two conversion modules
i.e.
,
l
-lysine conversion module and glutaric acid production module, were constructed on the basis of dual-vector systems to support high-level glutaric acid biosynthesis from glucose with reduced byproducts accumulation. Fed-batch fermentation of the final engineered strain harboring two conversion modules along with
Escherichia coli dapB
mut
(
dapB
C115G,G116C
) expression resulted in the production of 65.6 g L
−1
of glutaric acid in a 5 L fermenter. Furthermore, pilot-scale fed-batch fermentation was carried out in a 500 L fermenter to prepare large amounts of glutaric acid, resulting in the production of 56.0 g L
−1
of glutaric acid. Glutaric acid produced by the pilot-scale fermentation of
C. glutamicum
was purified and then used for the synthesis of dioctyl glutarate (DOG) which was further applied in PVC film plasticization and genuine leather coating. It was found that the physical properties of the PVC film plasticized by DOG were comparable to those of the PVC film plasticized by dioctyl succinate (DOS) and dioctyl adipate (DOA). Also, a nitrocellulose (NC) coating agent consisting of DOG could support similar flexibilities and abrasion resistance of leather to those treated with the NC coating agent consisting of DOS and DOA. These results suggest that bio-based DOG developed in this study can be considered as a promising plasticizer having comparable plasticization efficiencies with other aliphatic dicarboxylic acid ester plasticizers. |
| doi_str_mv | 10.1039/D1GC02686K |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2630948650</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2630948650</sourcerecordid><originalsourceid>FETCH-LOGICAL-c259t-6f0feba6b612bbf7c6b96c2629e4a6f8240e6b8efb5a4991197e713b0d71fd603</originalsourceid><addsrcrecordid>eNpFkN1KxDAQhYMouK7e-AQB74Rq0p-0uZSqq7jgjV6XJJ3sZmmbmh-hvoYvbJcVvZqBOfOdmYPQJSU3lGT89p6uapKyir0coQXNWZbwtCTHfz1LT9GZ9ztCKC1ZvkDf9_AJnR17GAK2GgssjU3UFnqjRIedjQFwsLgu8NgJH4wyX-Cwn4awBW88jt4MG7zpYhDOKCyUafHobBsVtFhOuIcgpO32tG7CMGzMAODmWW3dNIAUKoAzsT8gZtfYn6MTLToPF791id4fH97qp2T9unqu79aJSgseEqaJnveZZDSVUpeKSc7U_CKHXDBdpTkBJivQshA555TyEkqaSdKWVLeMZEt0deDO935E8KHZ2eiG2bJJWUZ4XrFir7o-qJSz3jvQzehML9zUUNLsQ2_-Q89-ACjLd-g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2630948650</pqid></control><display><type>article</type><title>Development of a bio-chemical route to C5 plasticizer synthesis using glutaric acid produced by metabolically engineered Corynebacterium glutamicum</title><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Sohn, Yu Jung ; Kang, Minsoo ; Ryu, Mi-Hee ; Lee, Siseon ; Kang, Kyoung Hee ; Hong, Yunjae ; Song, Bong Keun ; Park, Kyungmoon ; Park, Si Jae ; Joo, Jeong Chan ; Kim, Hee Taek</creator><creatorcontrib>Sohn, Yu Jung ; Kang, Minsoo ; Ryu, Mi-Hee ; Lee, Siseon ; Kang, Kyoung Hee ; Hong, Yunjae ; Song, Bong Keun ; Park, Kyungmoon ; Park, Si Jae ; Joo, Jeong Chan ; Kim, Hee Taek</creatorcontrib><description>Corynebacterium glutamicum
was engineered to produce glutaric acid by metabolic engineering approaches starting from the heterologous introduction of the glutaric acid biosynthesis pathway by the expression of
Pseudomonas putida davTDBA
genes. Two conversion modules
i.e.
,
l
-lysine conversion module and glutaric acid production module, were constructed on the basis of dual-vector systems to support high-level glutaric acid biosynthesis from glucose with reduced byproducts accumulation. Fed-batch fermentation of the final engineered strain harboring two conversion modules along with
Escherichia coli dapB
mut
(
dapB
C115G,G116C
) expression resulted in the production of 65.6 g L
−1
of glutaric acid in a 5 L fermenter. Furthermore, pilot-scale fed-batch fermentation was carried out in a 500 L fermenter to prepare large amounts of glutaric acid, resulting in the production of 56.0 g L
−1
of glutaric acid. Glutaric acid produced by the pilot-scale fermentation of
C. glutamicum
was purified and then used for the synthesis of dioctyl glutarate (DOG) which was further applied in PVC film plasticization and genuine leather coating. It was found that the physical properties of the PVC film plasticized by DOG were comparable to those of the PVC film plasticized by dioctyl succinate (DOS) and dioctyl adipate (DOA). Also, a nitrocellulose (NC) coating agent consisting of DOG could support similar flexibilities and abrasion resistance of leather to those treated with the NC coating agent consisting of DOS and DOA. These results suggest that bio-based DOG developed in this study can be considered as a promising plasticizer having comparable plasticization efficiencies with other aliphatic dicarboxylic acid ester plasticizers.</description><identifier>ISSN: 1463-9262</identifier><identifier>EISSN: 1463-9270</identifier><identifier>DOI: 10.1039/D1GC02686K</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Abrasion ; Abrasion resistant coatings ; Acid production ; Acids ; Bacteria ; Batch culture ; Biosynthesis ; Cellulose esters ; Cellulose nitrate ; Chemical synthesis ; Coatings ; Conversion ; Corynebacterium glutamicum ; Dicarboxylic acids ; Dioctyl adipate ; Dogs ; E coli ; Fed batch ; Fermentation ; Green chemistry ; Leather ; Lysine ; Metabolic engineering ; Modules ; Physical properties ; Plasticizers ; Pseudomonas putida</subject><ispartof>Green chemistry : an international journal and green chemistry resource : GC, 2022-02, Vol.24 (4), p.1590-1602</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c259t-6f0feba6b612bbf7c6b96c2629e4a6f8240e6b8efb5a4991197e713b0d71fd603</citedby><cites>FETCH-LOGICAL-c259t-6f0feba6b612bbf7c6b96c2629e4a6f8240e6b8efb5a4991197e713b0d71fd603</cites><orcidid>0000-0001-5393-7057 ; 0000-0001-9735-4582</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Sohn, Yu Jung</creatorcontrib><creatorcontrib>Kang, Minsoo</creatorcontrib><creatorcontrib>Ryu, Mi-Hee</creatorcontrib><creatorcontrib>Lee, Siseon</creatorcontrib><creatorcontrib>Kang, Kyoung Hee</creatorcontrib><creatorcontrib>Hong, Yunjae</creatorcontrib><creatorcontrib>Song, Bong Keun</creatorcontrib><creatorcontrib>Park, Kyungmoon</creatorcontrib><creatorcontrib>Park, Si Jae</creatorcontrib><creatorcontrib>Joo, Jeong Chan</creatorcontrib><creatorcontrib>Kim, Hee Taek</creatorcontrib><title>Development of a bio-chemical route to C5 plasticizer synthesis using glutaric acid produced by metabolically engineered Corynebacterium glutamicum</title><title>Green chemistry : an international journal and green chemistry resource : GC</title><description>Corynebacterium glutamicum
was engineered to produce glutaric acid by metabolic engineering approaches starting from the heterologous introduction of the glutaric acid biosynthesis pathway by the expression of
Pseudomonas putida davTDBA
genes. Two conversion modules
i.e.
,
l
-lysine conversion module and glutaric acid production module, were constructed on the basis of dual-vector systems to support high-level glutaric acid biosynthesis from glucose with reduced byproducts accumulation. Fed-batch fermentation of the final engineered strain harboring two conversion modules along with
Escherichia coli dapB
mut
(
dapB
C115G,G116C
) expression resulted in the production of 65.6 g L
−1
of glutaric acid in a 5 L fermenter. Furthermore, pilot-scale fed-batch fermentation was carried out in a 500 L fermenter to prepare large amounts of glutaric acid, resulting in the production of 56.0 g L
−1
of glutaric acid. Glutaric acid produced by the pilot-scale fermentation of
C. glutamicum
was purified and then used for the synthesis of dioctyl glutarate (DOG) which was further applied in PVC film plasticization and genuine leather coating. It was found that the physical properties of the PVC film plasticized by DOG were comparable to those of the PVC film plasticized by dioctyl succinate (DOS) and dioctyl adipate (DOA). Also, a nitrocellulose (NC) coating agent consisting of DOG could support similar flexibilities and abrasion resistance of leather to those treated with the NC coating agent consisting of DOS and DOA. These results suggest that bio-based DOG developed in this study can be considered as a promising plasticizer having comparable plasticization efficiencies with other aliphatic dicarboxylic acid ester plasticizers.</description><subject>Abrasion</subject><subject>Abrasion resistant coatings</subject><subject>Acid production</subject><subject>Acids</subject><subject>Bacteria</subject><subject>Batch culture</subject><subject>Biosynthesis</subject><subject>Cellulose esters</subject><subject>Cellulose nitrate</subject><subject>Chemical synthesis</subject><subject>Coatings</subject><subject>Conversion</subject><subject>Corynebacterium glutamicum</subject><subject>Dicarboxylic acids</subject><subject>Dioctyl adipate</subject><subject>Dogs</subject><subject>E coli</subject><subject>Fed batch</subject><subject>Fermentation</subject><subject>Green chemistry</subject><subject>Leather</subject><subject>Lysine</subject><subject>Metabolic engineering</subject><subject>Modules</subject><subject>Physical properties</subject><subject>Plasticizers</subject><subject>Pseudomonas putida</subject><issn>1463-9262</issn><issn>1463-9270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpFkN1KxDAQhYMouK7e-AQB74Rq0p-0uZSqq7jgjV6XJJ3sZmmbmh-hvoYvbJcVvZqBOfOdmYPQJSU3lGT89p6uapKyir0coQXNWZbwtCTHfz1LT9GZ9ztCKC1ZvkDf9_AJnR17GAK2GgssjU3UFnqjRIedjQFwsLgu8NgJH4wyX-Cwn4awBW88jt4MG7zpYhDOKCyUafHobBsVtFhOuIcgpO32tG7CMGzMAODmWW3dNIAUKoAzsT8gZtfYn6MTLToPF791id4fH97qp2T9unqu79aJSgseEqaJnveZZDSVUpeKSc7U_CKHXDBdpTkBJivQshA555TyEkqaSdKWVLeMZEt0deDO935E8KHZ2eiG2bJJWUZ4XrFir7o-qJSz3jvQzehML9zUUNLsQ2_-Q89-ACjLd-g</recordid><startdate>20220221</startdate><enddate>20220221</enddate><creator>Sohn, Yu Jung</creator><creator>Kang, Minsoo</creator><creator>Ryu, Mi-Hee</creator><creator>Lee, Siseon</creator><creator>Kang, Kyoung Hee</creator><creator>Hong, Yunjae</creator><creator>Song, Bong Keun</creator><creator>Park, Kyungmoon</creator><creator>Park, Si Jae</creator><creator>Joo, Jeong Chan</creator><creator>Kim, Hee Taek</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7ST</scope><scope>7U6</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0001-5393-7057</orcidid><orcidid>https://orcid.org/0000-0001-9735-4582</orcidid></search><sort><creationdate>20220221</creationdate><title>Development of a bio-chemical route to C5 plasticizer synthesis using glutaric acid produced by metabolically engineered Corynebacterium glutamicum</title><author>Sohn, Yu Jung ; Kang, Minsoo ; Ryu, Mi-Hee ; Lee, Siseon ; Kang, Kyoung Hee ; Hong, Yunjae ; Song, Bong Keun ; Park, Kyungmoon ; Park, Si Jae ; Joo, Jeong Chan ; Kim, Hee Taek</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c259t-6f0feba6b612bbf7c6b96c2629e4a6f8240e6b8efb5a4991197e713b0d71fd603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abrasion</topic><topic>Abrasion resistant coatings</topic><topic>Acid production</topic><topic>Acids</topic><topic>Bacteria</topic><topic>Batch culture</topic><topic>Biosynthesis</topic><topic>Cellulose esters</topic><topic>Cellulose nitrate</topic><topic>Chemical synthesis</topic><topic>Coatings</topic><topic>Conversion</topic><topic>Corynebacterium glutamicum</topic><topic>Dicarboxylic acids</topic><topic>Dioctyl adipate</topic><topic>Dogs</topic><topic>E coli</topic><topic>Fed batch</topic><topic>Fermentation</topic><topic>Green chemistry</topic><topic>Leather</topic><topic>Lysine</topic><topic>Metabolic engineering</topic><topic>Modules</topic><topic>Physical properties</topic><topic>Plasticizers</topic><topic>Pseudomonas putida</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sohn, Yu Jung</creatorcontrib><creatorcontrib>Kang, Minsoo</creatorcontrib><creatorcontrib>Ryu, Mi-Hee</creatorcontrib><creatorcontrib>Lee, Siseon</creatorcontrib><creatorcontrib>Kang, Kyoung Hee</creatorcontrib><creatorcontrib>Hong, Yunjae</creatorcontrib><creatorcontrib>Song, Bong Keun</creatorcontrib><creatorcontrib>Park, Kyungmoon</creatorcontrib><creatorcontrib>Park, Si Jae</creatorcontrib><creatorcontrib>Joo, Jeong Chan</creatorcontrib><creatorcontrib>Kim, Hee Taek</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Materials Research Database</collection><jtitle>Green chemistry : an international journal and green chemistry resource : GC</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sohn, Yu Jung</au><au>Kang, Minsoo</au><au>Ryu, Mi-Hee</au><au>Lee, Siseon</au><au>Kang, Kyoung Hee</au><au>Hong, Yunjae</au><au>Song, Bong Keun</au><au>Park, Kyungmoon</au><au>Park, Si Jae</au><au>Joo, Jeong Chan</au><au>Kim, Hee Taek</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a bio-chemical route to C5 plasticizer synthesis using glutaric acid produced by metabolically engineered Corynebacterium glutamicum</atitle><jtitle>Green chemistry : an international journal and green chemistry resource : GC</jtitle><date>2022-02-21</date><risdate>2022</risdate><volume>24</volume><issue>4</issue><spage>1590</spage><epage>1602</epage><pages>1590-1602</pages><issn>1463-9262</issn><eissn>1463-9270</eissn><abstract>Corynebacterium glutamicum
was engineered to produce glutaric acid by metabolic engineering approaches starting from the heterologous introduction of the glutaric acid biosynthesis pathway by the expression of
Pseudomonas putida davTDBA
genes. Two conversion modules
i.e.
,
l
-lysine conversion module and glutaric acid production module, were constructed on the basis of dual-vector systems to support high-level glutaric acid biosynthesis from glucose with reduced byproducts accumulation. Fed-batch fermentation of the final engineered strain harboring two conversion modules along with
Escherichia coli dapB
mut
(
dapB
C115G,G116C
) expression resulted in the production of 65.6 g L
−1
of glutaric acid in a 5 L fermenter. Furthermore, pilot-scale fed-batch fermentation was carried out in a 500 L fermenter to prepare large amounts of glutaric acid, resulting in the production of 56.0 g L
−1
of glutaric acid. Glutaric acid produced by the pilot-scale fermentation of
C. glutamicum
was purified and then used for the synthesis of dioctyl glutarate (DOG) which was further applied in PVC film plasticization and genuine leather coating. It was found that the physical properties of the PVC film plasticized by DOG were comparable to those of the PVC film plasticized by dioctyl succinate (DOS) and dioctyl adipate (DOA). Also, a nitrocellulose (NC) coating agent consisting of DOG could support similar flexibilities and abrasion resistance of leather to those treated with the NC coating agent consisting of DOS and DOA. These results suggest that bio-based DOG developed in this study can be considered as a promising plasticizer having comparable plasticization efficiencies with other aliphatic dicarboxylic acid ester plasticizers.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/D1GC02686K</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-5393-7057</orcidid><orcidid>https://orcid.org/0000-0001-9735-4582</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1463-9262 |
| ispartof | Green chemistry : an international journal and green chemistry resource : GC, 2022-02, Vol.24 (4), p.1590-1602 |
| issn | 1463-9262 1463-9270 |
| language | eng |
| recordid | cdi_proquest_journals_2630948650 |
| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Abrasion Abrasion resistant coatings Acid production Acids Bacteria Batch culture Biosynthesis Cellulose esters Cellulose nitrate Chemical synthesis Coatings Conversion Corynebacterium glutamicum Dicarboxylic acids Dioctyl adipate Dogs E coli Fed batch Fermentation Green chemistry Leather Lysine Metabolic engineering Modules Physical properties Plasticizers Pseudomonas putida |
| title | Development of a bio-chemical route to C5 plasticizer synthesis using glutaric acid produced by metabolically engineered Corynebacterium glutamicum |
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