Effect of polycation nanostructures on cell membrane permeability and toxicity
The interaction of nanometric synthetic materials with cell membranes is one of the key factors determining their possible cytotoxicity. This work investigated the interaction of polycation nanostructures with lipid and cellular membranes. The nanostructures (polymer micelles) were formed in aqueous...
Gespeichert in:
Veröffentlicht in: | Environmental science. Nano 2022-02, Vol.9 (2), p.72-713 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 713 |
---|---|
container_issue | 2 |
container_start_page | 72 |
container_title | Environmental science. Nano |
container_volume | 9 |
creator | Wytrwal-Sarna, Magdalena Knobloch, Paulina Lasota, S awomir Michalik, Marta Nowakowska, Maria Kepczynski, Mariusz |
description | The interaction of nanometric synthetic materials with cell membranes is one of the key factors determining their possible cytotoxicity. This work investigated the interaction of polycation nanostructures with lipid and cellular membranes. The nanostructures (polymer micelles) were formed in aqueous media as a result of the self-assembly of strong amphiphilic polycations containing hydrophobic alkyl groups of various lengths. The effect of alkyl length on micellization and its influence on the physicochemical properties and biological activity of the polycations were analyzed. Next, the cytotoxicity of the polycations was assessed using human skin fibroblasts (HSFs). The results show that the ability of the polycations to form pores in biomembranes decreases with the length of the attached alkyl groups. The polycations substituted with short alkyl groups were the most cytotoxic, which correlates well with their high capacity to open pores in biomembranes. However, they can perforate the fibroblast plasma membrane at non-toxic concentrations. Overall, our results show that the observed trends in cytotoxicity cannot be fully explained within simple interactions between polycationic micelles and cell membranes. A relationship does, however, seem to exist between polycation membrane activity (pore formation) and its cytotoxicity.
Amphiphilic polycations with quaternary ammonium groups show a strong tendency to porate lipid membranes. The polycations with a high capacity to open pores in biomembranes are the most cytotoxic to cells. |
doi_str_mv | 10.1039/d1en01156a |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2629455959</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2629455959</sourcerecordid><originalsourceid>FETCH-LOGICAL-c281t-263001e644af4d075561817c91355a99057ced4e1cd67a15d4a6a02a685a1d793</originalsourceid><addsrcrecordid>eNpFkN9LwzAQx4MoOHQvvgsB34RqLmnS5nHM-QPGfNHncktT6GiTmqTg_ns7J_Pp7r58uDs-hNwAewAm9GMN1jEAqfCMzDiTkJWg4PzUS3FJ5jHuGJsoLoUqZmSzahprEvUNHXy3N5ha76hD52MKo0ljsJFOibFdR3vbbwM6SwcbeovbtmvTnqKrafLfrZmGa3LRYBft_K9ekc_n1cfyNVu_v7wtF-vM8BJSxpWYfrAqz7HJa1ZIqaCEwmgQUqLWTBbG1rkFU6sCQdY5KmQcVSkR6kKLK3J33DsE_zXamKqdH4ObTlZccZ1LqeWBuj9SJvgYg22qIbQ9hn0FrDooq55gtflVtpjg2yMcojlx_0rFD7thZ1w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2629455959</pqid></control><display><type>article</type><title>Effect of polycation nanostructures on cell membrane permeability and toxicity</title><source>Royal Society Of Chemistry Journals</source><creator>Wytrwal-Sarna, Magdalena ; Knobloch, Paulina ; Lasota, S awomir ; Michalik, Marta ; Nowakowska, Maria ; Kepczynski, Mariusz</creator><creatorcontrib>Wytrwal-Sarna, Magdalena ; Knobloch, Paulina ; Lasota, S awomir ; Michalik, Marta ; Nowakowska, Maria ; Kepczynski, Mariusz</creatorcontrib><description>The interaction of nanometric synthetic materials with cell membranes is one of the key factors determining their possible cytotoxicity. This work investigated the interaction of polycation nanostructures with lipid and cellular membranes. The nanostructures (polymer micelles) were formed in aqueous media as a result of the self-assembly of strong amphiphilic polycations containing hydrophobic alkyl groups of various lengths. The effect of alkyl length on micellization and its influence on the physicochemical properties and biological activity of the polycations were analyzed. Next, the cytotoxicity of the polycations was assessed using human skin fibroblasts (HSFs). The results show that the ability of the polycations to form pores in biomembranes decreases with the length of the attached alkyl groups. The polycations substituted with short alkyl groups were the most cytotoxic, which correlates well with their high capacity to open pores in biomembranes. However, they can perforate the fibroblast plasma membrane at non-toxic concentrations. Overall, our results show that the observed trends in cytotoxicity cannot be fully explained within simple interactions between polycationic micelles and cell membranes. A relationship does, however, seem to exist between polycation membrane activity (pore formation) and its cytotoxicity.
Amphiphilic polycations with quaternary ammonium groups show a strong tendency to porate lipid membranes. The polycations with a high capacity to open pores in biomembranes are the most cytotoxic to cells.</description><identifier>ISSN: 2051-8153</identifier><identifier>EISSN: 2051-8161</identifier><identifier>DOI: 10.1039/d1en01156a</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Aqueous solutions ; Biological activity ; Biological properties ; Cell membranes ; Cytotoxicity ; Fibroblasts ; Hydrophobicity ; Lipids ; Membrane permeability ; Membranes ; Micelles ; Nanostructure ; Permeability ; Physicochemical processes ; Physicochemical properties ; Polycations ; Polyelectrolytes ; Polymers ; Pore formation ; Pores ; Self-assembly ; Toxicity</subject><ispartof>Environmental science. Nano, 2022-02, Vol.9 (2), p.72-713</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c281t-263001e644af4d075561817c91355a99057ced4e1cd67a15d4a6a02a685a1d793</citedby><cites>FETCH-LOGICAL-c281t-263001e644af4d075561817c91355a99057ced4e1cd67a15d4a6a02a685a1d793</cites><orcidid>0000-0001-6456-5463 ; 0000-0002-0906-4532</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Wytrwal-Sarna, Magdalena</creatorcontrib><creatorcontrib>Knobloch, Paulina</creatorcontrib><creatorcontrib>Lasota, S awomir</creatorcontrib><creatorcontrib>Michalik, Marta</creatorcontrib><creatorcontrib>Nowakowska, Maria</creatorcontrib><creatorcontrib>Kepczynski, Mariusz</creatorcontrib><title>Effect of polycation nanostructures on cell membrane permeability and toxicity</title><title>Environmental science. Nano</title><description>The interaction of nanometric synthetic materials with cell membranes is one of the key factors determining their possible cytotoxicity. This work investigated the interaction of polycation nanostructures with lipid and cellular membranes. The nanostructures (polymer micelles) were formed in aqueous media as a result of the self-assembly of strong amphiphilic polycations containing hydrophobic alkyl groups of various lengths. The effect of alkyl length on micellization and its influence on the physicochemical properties and biological activity of the polycations were analyzed. Next, the cytotoxicity of the polycations was assessed using human skin fibroblasts (HSFs). The results show that the ability of the polycations to form pores in biomembranes decreases with the length of the attached alkyl groups. The polycations substituted with short alkyl groups were the most cytotoxic, which correlates well with their high capacity to open pores in biomembranes. However, they can perforate the fibroblast plasma membrane at non-toxic concentrations. Overall, our results show that the observed trends in cytotoxicity cannot be fully explained within simple interactions between polycationic micelles and cell membranes. A relationship does, however, seem to exist between polycation membrane activity (pore formation) and its cytotoxicity.
Amphiphilic polycations with quaternary ammonium groups show a strong tendency to porate lipid membranes. The polycations with a high capacity to open pores in biomembranes are the most cytotoxic to cells.</description><subject>Aqueous solutions</subject><subject>Biological activity</subject><subject>Biological properties</subject><subject>Cell membranes</subject><subject>Cytotoxicity</subject><subject>Fibroblasts</subject><subject>Hydrophobicity</subject><subject>Lipids</subject><subject>Membrane permeability</subject><subject>Membranes</subject><subject>Micelles</subject><subject>Nanostructure</subject><subject>Permeability</subject><subject>Physicochemical processes</subject><subject>Physicochemical properties</subject><subject>Polycations</subject><subject>Polyelectrolytes</subject><subject>Polymers</subject><subject>Pore formation</subject><subject>Pores</subject><subject>Self-assembly</subject><subject>Toxicity</subject><issn>2051-8153</issn><issn>2051-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpFkN9LwzAQx4MoOHQvvgsB34RqLmnS5nHM-QPGfNHncktT6GiTmqTg_ns7J_Pp7r58uDs-hNwAewAm9GMN1jEAqfCMzDiTkJWg4PzUS3FJ5jHuGJsoLoUqZmSzahprEvUNHXy3N5ha76hD52MKo0ljsJFOibFdR3vbbwM6SwcbeovbtmvTnqKrafLfrZmGa3LRYBft_K9ekc_n1cfyNVu_v7wtF-vM8BJSxpWYfrAqz7HJa1ZIqaCEwmgQUqLWTBbG1rkFU6sCQdY5KmQcVSkR6kKLK3J33DsE_zXamKqdH4ObTlZccZ1LqeWBuj9SJvgYg22qIbQ9hn0FrDooq55gtflVtpjg2yMcojlx_0rFD7thZ1w</recordid><startdate>20220217</startdate><enddate>20220217</enddate><creator>Wytrwal-Sarna, Magdalena</creator><creator>Knobloch, Paulina</creator><creator>Lasota, S awomir</creator><creator>Michalik, Marta</creator><creator>Nowakowska, Maria</creator><creator>Kepczynski, Mariusz</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0001-6456-5463</orcidid><orcidid>https://orcid.org/0000-0002-0906-4532</orcidid></search><sort><creationdate>20220217</creationdate><title>Effect of polycation nanostructures on cell membrane permeability and toxicity</title><author>Wytrwal-Sarna, Magdalena ; Knobloch, Paulina ; Lasota, S awomir ; Michalik, Marta ; Nowakowska, Maria ; Kepczynski, Mariusz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-263001e644af4d075561817c91355a99057ced4e1cd67a15d4a6a02a685a1d793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aqueous solutions</topic><topic>Biological activity</topic><topic>Biological properties</topic><topic>Cell membranes</topic><topic>Cytotoxicity</topic><topic>Fibroblasts</topic><topic>Hydrophobicity</topic><topic>Lipids</topic><topic>Membrane permeability</topic><topic>Membranes</topic><topic>Micelles</topic><topic>Nanostructure</topic><topic>Permeability</topic><topic>Physicochemical processes</topic><topic>Physicochemical properties</topic><topic>Polycations</topic><topic>Polyelectrolytes</topic><topic>Polymers</topic><topic>Pore formation</topic><topic>Pores</topic><topic>Self-assembly</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wytrwal-Sarna, Magdalena</creatorcontrib><creatorcontrib>Knobloch, Paulina</creatorcontrib><creatorcontrib>Lasota, S awomir</creatorcontrib><creatorcontrib>Michalik, Marta</creatorcontrib><creatorcontrib>Nowakowska, Maria</creatorcontrib><creatorcontrib>Kepczynski, Mariusz</creatorcontrib><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><jtitle>Environmental science. Nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wytrwal-Sarna, Magdalena</au><au>Knobloch, Paulina</au><au>Lasota, S awomir</au><au>Michalik, Marta</au><au>Nowakowska, Maria</au><au>Kepczynski, Mariusz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of polycation nanostructures on cell membrane permeability and toxicity</atitle><jtitle>Environmental science. Nano</jtitle><date>2022-02-17</date><risdate>2022</risdate><volume>9</volume><issue>2</issue><spage>72</spage><epage>713</epage><pages>72-713</pages><issn>2051-8153</issn><eissn>2051-8161</eissn><abstract>The interaction of nanometric synthetic materials with cell membranes is one of the key factors determining their possible cytotoxicity. This work investigated the interaction of polycation nanostructures with lipid and cellular membranes. The nanostructures (polymer micelles) were formed in aqueous media as a result of the self-assembly of strong amphiphilic polycations containing hydrophobic alkyl groups of various lengths. The effect of alkyl length on micellization and its influence on the physicochemical properties and biological activity of the polycations were analyzed. Next, the cytotoxicity of the polycations was assessed using human skin fibroblasts (HSFs). The results show that the ability of the polycations to form pores in biomembranes decreases with the length of the attached alkyl groups. The polycations substituted with short alkyl groups were the most cytotoxic, which correlates well with their high capacity to open pores in biomembranes. However, they can perforate the fibroblast plasma membrane at non-toxic concentrations. Overall, our results show that the observed trends in cytotoxicity cannot be fully explained within simple interactions between polycationic micelles and cell membranes. A relationship does, however, seem to exist between polycation membrane activity (pore formation) and its cytotoxicity.
Amphiphilic polycations with quaternary ammonium groups show a strong tendency to porate lipid membranes. The polycations with a high capacity to open pores in biomembranes are the most cytotoxic to cells.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d1en01156a</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-6456-5463</orcidid><orcidid>https://orcid.org/0000-0002-0906-4532</orcidid></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2051-8153 |
ispartof | Environmental science. Nano, 2022-02, Vol.9 (2), p.72-713 |
issn | 2051-8153 2051-8161 |
language | eng |
recordid | cdi_proquest_journals_2629455959 |
source | Royal Society Of Chemistry Journals |
subjects | Aqueous solutions Biological activity Biological properties Cell membranes Cytotoxicity Fibroblasts Hydrophobicity Lipids Membrane permeability Membranes Micelles Nanostructure Permeability Physicochemical processes Physicochemical properties Polycations Polyelectrolytes Polymers Pore formation Pores Self-assembly Toxicity |
title | Effect of polycation nanostructures on cell membrane permeability and toxicity |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T01%3A42%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20polycation%20nanostructures%20on%20cell%20membrane%20permeability%20and%20toxicity&rft.jtitle=Environmental%20science.%20Nano&rft.au=Wytrwal-Sarna,%20Magdalena&rft.date=2022-02-17&rft.volume=9&rft.issue=2&rft.spage=72&rft.epage=713&rft.pages=72-713&rft.issn=2051-8153&rft.eissn=2051-8161&rft_id=info:doi/10.1039/d1en01156a&rft_dat=%3Cproquest_cross%3E2629455959%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2629455959&rft_id=info:pmid/&rfr_iscdi=true |