Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse
Doublesex-mab 3-related transcription factor 1 (DMRT1) has been identified as the first conserved gene involved in the testicular differentiation of vertebrates. However, the precise role of DMRT1 in spermatogenesis has not been made clear. In this study, immunohistochemical method was used to obser...
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| Veröffentlicht in: | International aquatic research 2014-06, Vol.6 (2), p.1, Article 68 |
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| creator | Kobayashi, Yasuhisa Nozu, Ryo Horiguchi, Ryo Nakamura, Masaru |
| description | Doublesex-mab 3-related transcription factor 1 (DMRT1) has been identified as the first conserved gene involved in the testicular differentiation of vertebrates. However, the precise role of DMRT1 in spermatogenesis has not been made clear. In this study, immunohistochemical method was used to observe DMRT1 protein localization in order to resolve cellular profile of DMRT1 in the adult testis of three-spot wrasse. DMRT1 protein was clearly and specifically localized in the Sertoli cells of all spermatogenic cells and epithelial cells comprising the efferent duct, but not in the germ cells. In addition, adult males were treated with aromatase inhibitor (AI) for investigating the role of estrogen on the transcription of DMRT1. AI treatment caused an increase in the levels of DMRT1 transcripts in the efferent duct region, concomitant with a decrease in spermatogonia and spermatocytes. |
| doi_str_mv | 10.1007/s40071-014-0068-4 |
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| subjects | Aquatic Pollution Aromatase Earth and Environmental Science Ecotoxicology Environment Epithelial cells Epithelium Estrogens Freshwater & Marine Ecology Gametocytes Germ cells Localization Marine & Freshwater Sciences Marine fishes Oestrogens Proteins Sertoli cells Sex hormones Short Communication Spermatocytes Spermatogenesis Spermatogonia Testes Transcription Transcription factors Vertebrates Waste Water Technology Water Water Management Water Pollution Control |
| title | Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse |
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