Comparative Transcriptome Analysis of Hybrid Population Provides Insights Into Starch Content in Sweet Potato (Ipomoea batatas L.) Storage Root
Sweet potato is an important starchy crop, which is mainly utilized as food, animal feed, and fermented to produce fuel-grade alcohol. However, the genetic mechanism regulating starch content in sweet potato is not yet fully understood. In this study, transcriptome analysis was performed in sweet po...
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| Veröffentlicht in: | Plant molecular biology reporter 2021-12, Vol.39 (4), p.673-684 |
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| creator | Qin, Zhen Li, Aixian Dong, Shunxu Wang, Qingmei Hou, Fuyun Zhang, Haiyan |
| description | Sweet potato is an important starchy crop, which is mainly utilized as food, animal feed, and fermented to produce fuel-grade alcohol. However, the genetic mechanism regulating starch content in sweet potato is not yet fully understood. In this study, transcriptome analysis was performed in sweet potato cultivars Luoxushu 8 (high starch), Zhengshu 20 (low starch), and 12 hybrid F
1
lines (six high-starch lines and six low-starch lines), which detected a total of 85,493 genes. The high and low-starch content groups were compared and found that 47 and 64 genes were up-regulated and down-regulated, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that seven differentially expressed genes were significantly enriched in the ATP-binding cassette (ABC) transporter pathway. Among them, five genes (annotated as ABCB1) that might involve in the auxin transport were significantly down-regulated in high-starch group. Meanwhile, the storage root sizes of Zhengshu 20 were higher as compared with Luoxushu 8. Simultaneously, four of the five differentially expressed genes enriched in the starch and sucrose metabolism pathway were up-regulated in high-starch group. These results provided the basis for the study of starch content in sweet potato storage root. |
| doi_str_mv | 10.1007/s11105-021-01282-x |
| format | Article |
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1
lines (six high-starch lines and six low-starch lines), which detected a total of 85,493 genes. The high and low-starch content groups were compared and found that 47 and 64 genes were up-regulated and down-regulated, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that seven differentially expressed genes were significantly enriched in the ATP-binding cassette (ABC) transporter pathway. Among them, five genes (annotated as ABCB1) that might involve in the auxin transport were significantly down-regulated in high-starch group. Meanwhile, the storage root sizes of Zhengshu 20 were higher as compared with Luoxushu 8. Simultaneously, four of the five differentially expressed genes enriched in the starch and sucrose metabolism pathway were up-regulated in high-starch group. These results provided the basis for the study of starch content in sweet potato storage root.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/s11105-021-01282-x</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animal feed ; Bioinformatics ; Biomedical and Life Sciences ; Cultivars ; Encyclopedias ; Feeds ; Fermented food ; Genes ; Genomes ; Ipomoea batatas ; Life Sciences ; Metabolomics ; Original Article ; Plant Breeding/Biotechnology ; Plant Sciences ; Potatoes ; Proteomics ; Starch ; Sucrose ; Sweet potatoes ; Transcriptomes ; Vegetables</subject><ispartof>Plant molecular biology reporter, 2021-12, Vol.39 (4), p.673-684</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature 2021. corrected publication, 2021</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature 2021. corrected publication, 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-a7e3c9164c6fae560e12baba70e77f455eadd8ef568e116dd1ec0ac743efda9e3</citedby><cites>FETCH-LOGICAL-c319t-a7e3c9164c6fae560e12baba70e77f455eadd8ef568e116dd1ec0ac743efda9e3</cites><orcidid>0000-0002-1690-5942</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11105-021-01282-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11105-021-01282-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Qin, Zhen</creatorcontrib><creatorcontrib>Li, Aixian</creatorcontrib><creatorcontrib>Dong, Shunxu</creatorcontrib><creatorcontrib>Wang, Qingmei</creatorcontrib><creatorcontrib>Hou, Fuyun</creatorcontrib><creatorcontrib>Zhang, Haiyan</creatorcontrib><title>Comparative Transcriptome Analysis of Hybrid Population Provides Insights Into Starch Content in Sweet Potato (Ipomoea batatas L.) Storage Root</title><title>Plant molecular biology reporter</title><addtitle>Plant Mol Biol Rep</addtitle><description>Sweet potato is an important starchy crop, which is mainly utilized as food, animal feed, and fermented to produce fuel-grade alcohol. However, the genetic mechanism regulating starch content in sweet potato is not yet fully understood. In this study, transcriptome analysis was performed in sweet potato cultivars Luoxushu 8 (high starch), Zhengshu 20 (low starch), and 12 hybrid F
1
lines (six high-starch lines and six low-starch lines), which detected a total of 85,493 genes. The high and low-starch content groups were compared and found that 47 and 64 genes were up-regulated and down-regulated, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that seven differentially expressed genes were significantly enriched in the ATP-binding cassette (ABC) transporter pathway. Among them, five genes (annotated as ABCB1) that might involve in the auxin transport were significantly down-regulated in high-starch group. Meanwhile, the storage root sizes of Zhengshu 20 were higher as compared with Luoxushu 8. Simultaneously, four of the five differentially expressed genes enriched in the starch and sucrose metabolism pathway were up-regulated in high-starch group. These results provided the basis for the study of starch content in sweet potato storage root.</description><subject>Animal feed</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Cultivars</subject><subject>Encyclopedias</subject><subject>Feeds</subject><subject>Fermented food</subject><subject>Genes</subject><subject>Genomes</subject><subject>Ipomoea batatas</subject><subject>Life Sciences</subject><subject>Metabolomics</subject><subject>Original Article</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Sciences</subject><subject>Potatoes</subject><subject>Proteomics</subject><subject>Starch</subject><subject>Sucrose</subject><subject>Sweet potatoes</subject><subject>Transcriptomes</subject><subject>Vegetables</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kM9OAjEQhxujiYi-gKcmXvSw2On-PxKiQkIiETw3w-4sLIHt2haEp_CVLWLizdPMZL5vkvkxdguiB0KkjxYARBwICYEAmclgf8Y6EKcyyDPIzllHpGEc5EkkLtmVtSvhJZFlHfY10JsWDbp6R3xmsLGFqVunN8T7Da4PtrZcV3x4mJu65BPdbtee1Q2fGL2rS7J81Nh6sXTHxmk-dWiKJR_oxlHjeN3w6SeR86ZDv74ftXqjCfkc_YyWj3sP3tEGF8TftHbX7KLCtaWb39pl789Ps8EwGL--jAb9cVCEkLsAUwqLHJKoSCqkOBEEco5zTAWlaRXFMWFZZlTFSUYASVkCFQKLNAqpKjGnsMvuTndboz-2ZJ1a6a3xH1slEwmRjCMReUqeqMJoaw1VqjX1Bs1BgVDH4NUpeOWDVz_Bq72XwpNkPdwsyPyd_sf6Biqmico</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Qin, Zhen</creator><creator>Li, Aixian</creator><creator>Dong, Shunxu</creator><creator>Wang, Qingmei</creator><creator>Hou, Fuyun</creator><creator>Zhang, Haiyan</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-1690-5942</orcidid></search><sort><creationdate>20211201</creationdate><title>Comparative Transcriptome Analysis of Hybrid Population Provides Insights Into Starch Content in Sweet Potato (Ipomoea batatas L.) Storage Root</title><author>Qin, Zhen ; Li, Aixian ; Dong, Shunxu ; Wang, Qingmei ; Hou, Fuyun ; Zhang, Haiyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-a7e3c9164c6fae560e12baba70e77f455eadd8ef568e116dd1ec0ac743efda9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal feed</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Cultivars</topic><topic>Encyclopedias</topic><topic>Feeds</topic><topic>Fermented food</topic><topic>Genes</topic><topic>Genomes</topic><topic>Ipomoea batatas</topic><topic>Life Sciences</topic><topic>Metabolomics</topic><topic>Original Article</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Sciences</topic><topic>Potatoes</topic><topic>Proteomics</topic><topic>Starch</topic><topic>Sucrose</topic><topic>Sweet potatoes</topic><topic>Transcriptomes</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qin, Zhen</creatorcontrib><creatorcontrib>Li, Aixian</creatorcontrib><creatorcontrib>Dong, Shunxu</creatorcontrib><creatorcontrib>Wang, Qingmei</creatorcontrib><creatorcontrib>Hou, Fuyun</creatorcontrib><creatorcontrib>Zhang, Haiyan</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qin, Zhen</au><au>Li, Aixian</au><au>Dong, Shunxu</au><au>Wang, Qingmei</au><au>Hou, Fuyun</au><au>Zhang, Haiyan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Transcriptome Analysis of Hybrid Population Provides Insights Into Starch Content in Sweet Potato (Ipomoea batatas L.) Storage Root</atitle><jtitle>Plant molecular biology reporter</jtitle><stitle>Plant Mol Biol Rep</stitle><date>2021-12-01</date><risdate>2021</risdate><volume>39</volume><issue>4</issue><spage>673</spage><epage>684</epage><pages>673-684</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>Sweet potato is an important starchy crop, which is mainly utilized as food, animal feed, and fermented to produce fuel-grade alcohol. However, the genetic mechanism regulating starch content in sweet potato is not yet fully understood. In this study, transcriptome analysis was performed in sweet potato cultivars Luoxushu 8 (high starch), Zhengshu 20 (low starch), and 12 hybrid F
1
lines (six high-starch lines and six low-starch lines), which detected a total of 85,493 genes. The high and low-starch content groups were compared and found that 47 and 64 genes were up-regulated and down-regulated, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that seven differentially expressed genes were significantly enriched in the ATP-binding cassette (ABC) transporter pathway. Among them, five genes (annotated as ABCB1) that might involve in the auxin transport were significantly down-regulated in high-starch group. Meanwhile, the storage root sizes of Zhengshu 20 were higher as compared with Luoxushu 8. Simultaneously, four of the five differentially expressed genes enriched in the starch and sucrose metabolism pathway were up-regulated in high-starch group. These results provided the basis for the study of starch content in sweet potato storage root.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11105-021-01282-x</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-1690-5942</orcidid></addata></record> |
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| subjects | Animal feed Bioinformatics Biomedical and Life Sciences Cultivars Encyclopedias Feeds Fermented food Genes Genomes Ipomoea batatas Life Sciences Metabolomics Original Article Plant Breeding/Biotechnology Plant Sciences Potatoes Proteomics Starch Sucrose Sweet potatoes Transcriptomes Vegetables |
| title | Comparative Transcriptome Analysis of Hybrid Population Provides Insights Into Starch Content in Sweet Potato (Ipomoea batatas L.) Storage Root |
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