The Optimization of Protease Enzyme Extraction From Streblus Asper (Kesinai)

The Optimization of Protease Enzyme Extraction From Streblus Asper (Kesinai)

Protease from Streblus asper (Kesinai) is an interesting rennet substitute and yet very few studies had been conducted so far. In the present study, the leaf extract of Kesinai had been discovered to investigate the ability of this milk coagulating enzyme. The development of the optimized conditions...

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Veröffentlicht in:IOP conference series. Materials Science and Engineering 2020-09, Vol.932 (1), p.12039
Hauptverfasser: Seow, Yen Yi, Abdullah, Rozaini, Lee, Yen Fong, Zakaria, Zarina, Hassan, Huzairy, Syed Zuber, Sharifah Zati Hanani, Mohd Ruslan, Mahfuz Affif
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Buffers
Enzymes
Optimization
Protease
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container_title IOP conference series. Materials Science and Engineering
container_volume 932
creator Seow, Yen Yi
Abdullah, Rozaini
Lee, Yen Fong
Zakaria, Zarina
Hassan, Huzairy
Syed Zuber, Sharifah Zati Hanani
Mohd Ruslan, Mahfuz Affif
description Protease from Streblus asper (Kesinai) is an interesting rennet substitute and yet very few studies had been conducted so far. In the present study, the leaf extract of Kesinai had been discovered to investigate the ability of this milk coagulating enzyme. The development of the optimized conditions for enzyme extraction was analyzed by using Central Composite Design (CCD). The studied factors were ratio of sample to buffer, weight of sample (g) and homogenization time (min). It was found that a 30 g of S. asper leaves sample with the ratio of the sample to buffer of 1:1 and at a mixing rate of 2 minutes established the most desirable conditions for serine proteases extraction from the S. asper leaves sample.
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subjects Buffers
Enzymes
Optimization
Protease
title The Optimization of Protease Enzyme Extraction From Streblus Asper (Kesinai)
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