Surface and stability analysis of immobilized laccase on poly(ethylene terephthalate) grafted maleic anhydride nanofiber mat
BACKGROUND A poly(ethylene terephthalate) (PET) based carrier was used for enzyme immobilization due to its good physical and mechanical properties. However, its high hydrophobicity and chemically inactive properties hindered its capability as an enzyme carrier. This study developed a PET‐based carr...
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| Veröffentlicht in: | Journal of chemical technology and biotechnology (1986) 2022-01, Vol.97 (1), p.140-146 |
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| container_title | Journal of chemical technology and biotechnology (1986) |
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| creator | Mohd Syukri, Mohd Syahlan Mohamad, Zurina A Rahman, Roshanida Illias, Rosli Md Tokuyama, Hideaki |
| description | BACKGROUND
A poly(ethylene terephthalate) (PET) based carrier was used for enzyme immobilization due to its good physical and mechanical properties. However, its high hydrophobicity and chemically inactive properties hindered its capability as an enzyme carrier. This study developed a PET‐based carrier by grafting PET with maleic anhydride (MAH) before electrospinning. This carrier was used to immobilize laccase and characterized based on its stability after immobilization.
RESULTS
The highest laccase activity recovery for laccase immobilized on PET‐g‐MAH nanofiber mat was 59.17% using covalent bonding, followed by the crosslinking method. The static water contact angle of PET‐g‐MAH nanofiber mats was reduced from 129 ± 8o to 109 ± 12o. Based on the Wenzel model, the reduction was due to the decrease in surface roughness of the hydrophobic surface. Besides, the immobilized laccase also withstood high temperature up to 60 °C and retained about 29.22% ± 5.06% of its initial activity after 30 days of storage and repeated use.
CONCLUSION
Laccase immobilization on PET‐g‐MAH nanofiber has good stability (thermal, storage and reusability) and is recommended to be implemented in the industries. © 2021 Society of Chemical Industry (SCI). |
| doi_str_mv | 10.1002/jctb.6919 |
| format | Article |
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A poly(ethylene terephthalate) (PET) based carrier was used for enzyme immobilization due to its good physical and mechanical properties. However, its high hydrophobicity and chemically inactive properties hindered its capability as an enzyme carrier. This study developed a PET‐based carrier by grafting PET with maleic anhydride (MAH) before electrospinning. This carrier was used to immobilize laccase and characterized based on its stability after immobilization.
RESULTS
The highest laccase activity recovery for laccase immobilized on PET‐g‐MAH nanofiber mat was 59.17% using covalent bonding, followed by the crosslinking method. The static water contact angle of PET‐g‐MAH nanofiber mats was reduced from 129 ± 8o to 109 ± 12o. Based on the Wenzel model, the reduction was due to the decrease in surface roughness of the hydrophobic surface. Besides, the immobilized laccase also withstood high temperature up to 60 °C and retained about 29.22% ± 5.06% of its initial activity after 30 days of storage and repeated use.
CONCLUSION
Laccase immobilization on PET‐g‐MAH nanofiber has good stability (thermal, storage and reusability) and is recommended to be implemented in the industries. © 2021 Society of Chemical Industry (SCI).</description><identifier>ISSN: 0268-2575</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/jctb.6919</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Contact angle ; Crosslinking ; enzyme ; Enzymes ; Ethylene ; grafting ; High temperature ; Hydrophobicity ; Immobilization ; Laccase ; Maleic anhydride ; Mechanical properties ; nanofiber ; Nanofibers ; Physical properties ; poly(ethylene terephthalate) ; Polyethylene terephthalate ; Shelf life ; Stability analysis ; Surface roughness ; Surface stability</subject><ispartof>Journal of chemical technology and biotechnology (1986), 2022-01, Vol.97 (1), p.140-146</ispartof><rights>2021 Society of Chemical Industry (SCI).</rights><rights>Copyright © 2022 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2979-3393098c0ff11733c78804dca16e9739faa6e974ab8721914ea48c54f2a032c63</citedby><cites>FETCH-LOGICAL-c2979-3393098c0ff11733c78804dca16e9739faa6e974ab8721914ea48c54f2a032c63</cites><orcidid>0000-0003-4640-2614 ; 0000-0001-7153-5043 ; 0000-0003-2603-0390</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjctb.6919$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjctb.6919$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids></links><search><creatorcontrib>Mohd Syukri, Mohd Syahlan</creatorcontrib><creatorcontrib>Mohamad, Zurina</creatorcontrib><creatorcontrib>A Rahman, Roshanida</creatorcontrib><creatorcontrib>Illias, Rosli Md</creatorcontrib><creatorcontrib>Tokuyama, Hideaki</creatorcontrib><title>Surface and stability analysis of immobilized laccase on poly(ethylene terephthalate) grafted maleic anhydride nanofiber mat</title><title>Journal of chemical technology and biotechnology (1986)</title><description>BACKGROUND
A poly(ethylene terephthalate) (PET) based carrier was used for enzyme immobilization due to its good physical and mechanical properties. However, its high hydrophobicity and chemically inactive properties hindered its capability as an enzyme carrier. This study developed a PET‐based carrier by grafting PET with maleic anhydride (MAH) before electrospinning. This carrier was used to immobilize laccase and characterized based on its stability after immobilization.
RESULTS
The highest laccase activity recovery for laccase immobilized on PET‐g‐MAH nanofiber mat was 59.17% using covalent bonding, followed by the crosslinking method. The static water contact angle of PET‐g‐MAH nanofiber mats was reduced from 129 ± 8o to 109 ± 12o. Based on the Wenzel model, the reduction was due to the decrease in surface roughness of the hydrophobic surface. Besides, the immobilized laccase also withstood high temperature up to 60 °C and retained about 29.22% ± 5.06% of its initial activity after 30 days of storage and repeated use.
CONCLUSION
Laccase immobilization on PET‐g‐MAH nanofiber has good stability (thermal, storage and reusability) and is recommended to be implemented in the industries. © 2021 Society of Chemical Industry (SCI).</description><subject>Contact angle</subject><subject>Crosslinking</subject><subject>enzyme</subject><subject>Enzymes</subject><subject>Ethylene</subject><subject>grafting</subject><subject>High temperature</subject><subject>Hydrophobicity</subject><subject>Immobilization</subject><subject>Laccase</subject><subject>Maleic anhydride</subject><subject>Mechanical properties</subject><subject>nanofiber</subject><subject>Nanofibers</subject><subject>Physical properties</subject><subject>poly(ethylene terephthalate)</subject><subject>Polyethylene terephthalate</subject><subject>Shelf life</subject><subject>Stability analysis</subject><subject>Surface roughness</subject><subject>Surface stability</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kE9PwzAMxSMEEmNw4BtE4sIO3ZK0S5sjTPzVJA6Mc-WmDsuUtSPJhIr48LSMKyfbzz8_yY-QS86mnDEx2-hYTaXi6oiMOFN5kknJjsmICVkkYp7PT8lZCBvGmCyEHJHv1703oJFCU9MQobLOxq6fwHXBBtoaarfbdpC_sKYOtIaAtG3ornXdNcZ157BBGtHjbh3X4CDihL57MLHnt-DQ6t5u3dXe1kgbaFpjK_T9Kp6TEwMu4MVfHZO3-7vV4jFZvjw8LW6WiRYqV0maqpSpQjNjOM_TVOdFwbJaA5eo8lQZgKHJoCpywRXPELJCzzMjgKVCy3RMrg6-O99-7DHEctPuff9iKIXkhRCZYKqnJgdK-zYEj6bcebsF35WclUO45RBuOYTbs7MD-2kddv-D5fNidft78QOdOH13</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Mohd Syukri, Mohd Syahlan</creator><creator>Mohamad, Zurina</creator><creator>A Rahman, Roshanida</creator><creator>Illias, Rosli Md</creator><creator>Tokuyama, Hideaki</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0003-4640-2614</orcidid><orcidid>https://orcid.org/0000-0001-7153-5043</orcidid><orcidid>https://orcid.org/0000-0003-2603-0390</orcidid></search><sort><creationdate>202201</creationdate><title>Surface and stability analysis of immobilized laccase on poly(ethylene terephthalate) grafted maleic anhydride nanofiber mat</title><author>Mohd Syukri, Mohd Syahlan ; Mohamad, Zurina ; A Rahman, Roshanida ; Illias, Rosli Md ; Tokuyama, Hideaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2979-3393098c0ff11733c78804dca16e9739faa6e974ab8721914ea48c54f2a032c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Contact angle</topic><topic>Crosslinking</topic><topic>enzyme</topic><topic>Enzymes</topic><topic>Ethylene</topic><topic>grafting</topic><topic>High temperature</topic><topic>Hydrophobicity</topic><topic>Immobilization</topic><topic>Laccase</topic><topic>Maleic anhydride</topic><topic>Mechanical properties</topic><topic>nanofiber</topic><topic>Nanofibers</topic><topic>Physical properties</topic><topic>poly(ethylene terephthalate)</topic><topic>Polyethylene terephthalate</topic><topic>Shelf life</topic><topic>Stability analysis</topic><topic>Surface roughness</topic><topic>Surface stability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohd Syukri, Mohd Syahlan</creatorcontrib><creatorcontrib>Mohamad, Zurina</creatorcontrib><creatorcontrib>A Rahman, Roshanida</creatorcontrib><creatorcontrib>Illias, Rosli Md</creatorcontrib><creatorcontrib>Tokuyama, Hideaki</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohd Syukri, Mohd Syahlan</au><au>Mohamad, Zurina</au><au>A Rahman, Roshanida</au><au>Illias, Rosli Md</au><au>Tokuyama, Hideaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface and stability analysis of immobilized laccase on poly(ethylene terephthalate) grafted maleic anhydride nanofiber mat</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><date>2022-01</date><risdate>2022</risdate><volume>97</volume><issue>1</issue><spage>140</spage><epage>146</epage><pages>140-146</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><abstract>BACKGROUND
A poly(ethylene terephthalate) (PET) based carrier was used for enzyme immobilization due to its good physical and mechanical properties. However, its high hydrophobicity and chemically inactive properties hindered its capability as an enzyme carrier. This study developed a PET‐based carrier by grafting PET with maleic anhydride (MAH) before electrospinning. This carrier was used to immobilize laccase and characterized based on its stability after immobilization.
RESULTS
The highest laccase activity recovery for laccase immobilized on PET‐g‐MAH nanofiber mat was 59.17% using covalent bonding, followed by the crosslinking method. The static water contact angle of PET‐g‐MAH nanofiber mats was reduced from 129 ± 8o to 109 ± 12o. Based on the Wenzel model, the reduction was due to the decrease in surface roughness of the hydrophobic surface. Besides, the immobilized laccase also withstood high temperature up to 60 °C and retained about 29.22% ± 5.06% of its initial activity after 30 days of storage and repeated use.
CONCLUSION
Laccase immobilization on PET‐g‐MAH nanofiber has good stability (thermal, storage and reusability) and is recommended to be implemented in the industries. © 2021 Society of Chemical Industry (SCI).</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jctb.6919</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-4640-2614</orcidid><orcidid>https://orcid.org/0000-0001-7153-5043</orcidid><orcidid>https://orcid.org/0000-0003-2603-0390</orcidid></addata></record> |
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| ispartof | Journal of chemical technology and biotechnology (1986), 2022-01, Vol.97 (1), p.140-146 |
| issn | 0268-2575 1097-4660 |
| language | eng |
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| source | Wiley Online Library All Journals |
| subjects | Contact angle Crosslinking enzyme Enzymes Ethylene grafting High temperature Hydrophobicity Immobilization Laccase Maleic anhydride Mechanical properties nanofiber Nanofibers Physical properties poly(ethylene terephthalate) Polyethylene terephthalate Shelf life Stability analysis Surface roughness Surface stability |
| title | Surface and stability analysis of immobilized laccase on poly(ethylene terephthalate) grafted maleic anhydride nanofiber mat |
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