DNA metabarcoding dietary analyses of the wood mouse Apodemus speciosus on Innoshima Island, Japan, and implications for primer choice
We used DNA metabarcoding to assess the seasonal diets of the large Japanese wood mouse, Apodemus speciosus (Muridae, Rodentia), in forest edges adjacent to citrus orchards on Innoshima Island, Japan. We used one chloroplast and three mitochondrial DNA barcoding markers to determine mouse diets. Amo...
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| Veröffentlicht in: | Mammal research 2022, Vol.67 (1), p.109-122 |
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| creator | Sato, Jun J. Ohtsuki, Yuya Nishiura, Naoki Mouri, Kumi |
| description | We used DNA metabarcoding to assess the seasonal diets of the large Japanese wood mouse,
Apodemus speciosus
(Muridae, Rodentia), in forest edges adjacent to citrus orchards on Innoshima Island, Japan. We used one chloroplast and three mitochondrial DNA barcoding markers to determine mouse diets. Among the various plant and invertebrate diets,
A. speciosus
typically consumed Chinese cork oak (
Quercus variabilis
) in early spring (likely acorns preserved during winter) and gypsy moths (
Lymantria dispar
, a forest pest) in late spring and summer. In addition, we found that
A. speciosus
also preyed on orchard pests, including the gutta stink bug and other potentially harmful invertebrates. The season during which
A. speciosus
preyed on stink bugs corresponded with the harvest of orchard products. This study revealed several of the ecological roles of
A. speciosus
within the boundary zone between forest and human ecosystems. Furthermore, based on the performance of various mitochondrial markers in dietary profiling of invertebrate food items, we recommend the multi-locus DNA metabarcoding method to comprehensively assess the diet of
A. speciosus
. |
| doi_str_mv | 10.1007/s13364-021-00601-7 |
| format | Article |
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Apodemus speciosus
(Muridae, Rodentia), in forest edges adjacent to citrus orchards on Innoshima Island, Japan. We used one chloroplast and three mitochondrial DNA barcoding markers to determine mouse diets. Among the various plant and invertebrate diets,
A. speciosus
typically consumed Chinese cork oak (
Quercus variabilis
) in early spring (likely acorns preserved during winter) and gypsy moths (
Lymantria dispar
, a forest pest) in late spring and summer. In addition, we found that
A. speciosus
also preyed on orchard pests, including the gutta stink bug and other potentially harmful invertebrates. The season during which
A. speciosus
preyed on stink bugs corresponded with the harvest of orchard products. This study revealed several of the ecological roles of
A. speciosus
within the boundary zone between forest and human ecosystems. Furthermore, based on the performance of various mitochondrial markers in dietary profiling of invertebrate food items, we recommend the multi-locus DNA metabarcoding method to comprehensively assess the diet of
A. speciosus
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Apodemus speciosus
(Muridae, Rodentia), in forest edges adjacent to citrus orchards on Innoshima Island, Japan. We used one chloroplast and three mitochondrial DNA barcoding markers to determine mouse diets. Among the various plant and invertebrate diets,
A. speciosus
typically consumed Chinese cork oak (
Quercus variabilis
) in early spring (likely acorns preserved during winter) and gypsy moths (
Lymantria dispar
, a forest pest) in late spring and summer. In addition, we found that
A. speciosus
also preyed on orchard pests, including the gutta stink bug and other potentially harmful invertebrates. The season during which
A. speciosus
preyed on stink bugs corresponded with the harvest of orchard products. This study revealed several of the ecological roles of
A. speciosus
within the boundary zone between forest and human ecosystems. Furthermore, based on the performance of various mitochondrial markers in dietary profiling of invertebrate food items, we recommend the multi-locus DNA metabarcoding method to comprehensively assess the diet of
A. speciosus
.</description><subject>Animal Ecology</subject><subject>Apodemus speciosus</subject><subject>Biomedical and Life Sciences</subject><subject>Chloroplast DNA</subject><subject>Chloroplasts</subject><subject>Diet</subject><subject>DNA barcoding</subject><subject>Evolutionary Biology</subject><subject>Fish & Wildlife Biology & Management</subject><subject>Invertebrates</subject><subject>Life Sciences</subject><subject>Lymantria dispar</subject><subject>Mitochondrial DNA</subject><subject>Orchards</subject><subject>Original Paper</subject><subject>Pests</subject><subject>Quercus variabilis</subject><subject>Wood</subject><subject>Zoology</subject><issn>2199-2401</issn><issn>2199-241X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kM1OwzAQhCMEEhX0BThZ4tqAfxInPlblr6iCC0jcLMd2WleNHbypUF-A58alCG6cdleaGe18WXZB8BXBuLoGwhgvckxJjjHHJK-OshElQuS0IG_Hvzsmp9kYYI0xJlVBqWCj7PPmaYo6O6hGRR2M80tkXDrjDimvNjuwgEKLhpVFHyEY1IUtWDTtg7HdFhD0VrsAaQsezb0PsHKdQnPYKG8m6FH1yk9SkkGu6zdOq8EFD6gNEfXRdTYivQpO2_PspFUbsOOfeZa93t2-zB7yxfP9fDZd5JpxNuSKt9YKI7QoWGVSWcqbktZNy0XR1ExhUZeGllZo0xKjSa1KXGHaWNISygVlZ9nlIbeP4X1rYZDrsI2pKEjKCWc1KXiZVPSg0jEARNvK_bOJiSRY7pHLA3KZkMtv5LJKJnYwQRL7pY1_0f-4vgBAuoUF</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Sato, Jun J.</creator><creator>Ohtsuki, Yuya</creator><creator>Nishiura, Naoki</creator><creator>Mouri, Kumi</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-4934-2795</orcidid></search><sort><creationdate>2022</creationdate><title>DNA metabarcoding dietary analyses of the wood mouse Apodemus speciosus on Innoshima Island, Japan, and implications for primer choice</title><author>Sato, Jun J. ; Ohtsuki, Yuya ; Nishiura, Naoki ; Mouri, Kumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-a6fee9d9c9437d00626b528bf694b83a0985d25e9cdf1dc18a50702be1f126923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal Ecology</topic><topic>Apodemus speciosus</topic><topic>Biomedical and Life Sciences</topic><topic>Chloroplast DNA</topic><topic>Chloroplasts</topic><topic>Diet</topic><topic>DNA barcoding</topic><topic>Evolutionary Biology</topic><topic>Fish & Wildlife Biology & Management</topic><topic>Invertebrates</topic><topic>Life Sciences</topic><topic>Lymantria dispar</topic><topic>Mitochondrial DNA</topic><topic>Orchards</topic><topic>Original Paper</topic><topic>Pests</topic><topic>Quercus variabilis</topic><topic>Wood</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sato, Jun J.</creatorcontrib><creatorcontrib>Ohtsuki, Yuya</creatorcontrib><creatorcontrib>Nishiura, Naoki</creatorcontrib><creatorcontrib>Mouri, Kumi</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><jtitle>Mammal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sato, Jun J.</au><au>Ohtsuki, Yuya</au><au>Nishiura, Naoki</au><au>Mouri, Kumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA metabarcoding dietary analyses of the wood mouse Apodemus speciosus on Innoshima Island, Japan, and implications for primer choice</atitle><jtitle>Mammal research</jtitle><stitle>Mamm Res</stitle><date>2022</date><risdate>2022</risdate><volume>67</volume><issue>1</issue><spage>109</spage><epage>122</epage><pages>109-122</pages><issn>2199-2401</issn><eissn>2199-241X</eissn><abstract>We used DNA metabarcoding to assess the seasonal diets of the large Japanese wood mouse,
Apodemus speciosus
(Muridae, Rodentia), in forest edges adjacent to citrus orchards on Innoshima Island, Japan. We used one chloroplast and three mitochondrial DNA barcoding markers to determine mouse diets. Among the various plant and invertebrate diets,
A. speciosus
typically consumed Chinese cork oak (
Quercus variabilis
) in early spring (likely acorns preserved during winter) and gypsy moths (
Lymantria dispar
, a forest pest) in late spring and summer. In addition, we found that
A. speciosus
also preyed on orchard pests, including the gutta stink bug and other potentially harmful invertebrates. The season during which
A. speciosus
preyed on stink bugs corresponded with the harvest of orchard products. This study revealed several of the ecological roles of
A. speciosus
within the boundary zone between forest and human ecosystems. Furthermore, based on the performance of various mitochondrial markers in dietary profiling of invertebrate food items, we recommend the multi-locus DNA metabarcoding method to comprehensively assess the diet of
A. speciosus
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s13364-021-00601-7</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-4934-2795</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Animal Ecology Apodemus speciosus Biomedical and Life Sciences Chloroplast DNA Chloroplasts Diet DNA barcoding Evolutionary Biology Fish & Wildlife Biology & Management Invertebrates Life Sciences Lymantria dispar Mitochondrial DNA Orchards Original Paper Pests Quercus variabilis Wood Zoology |
| title | DNA metabarcoding dietary analyses of the wood mouse Apodemus speciosus on Innoshima Island, Japan, and implications for primer choice |
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