Antibacterial, antibiofilm, and anti-adhesion activities of Piperbetle leaf extract against Avian pathogenic Escherichiacoli
Piper betle leaves have traditionally been used to treat many diseases, including bacterial infections. The present study aimed to investigate the antibacterial, antibiofilm, and anti-adhesion activities of P. betle extract against avian pathogenic Escherichia coli (APEC). The ethanol extract of P....
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| Veröffentlicht in: | Archives of microbiology 2022, Vol.204 (1) |
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| creator | Kulnanan, Pawinee Chuprom, Julalak Thomrongsuwannakij, Thotsapol Romyasamit, Chonticha Sangkanu, Suthinee Manin, Nannaphat Nissapatorn, Veeranoot de Lourdes Pereira, Maria Wilairatana, Polrat Kitpipit, Warangkana Mitsuwan, Watcharapong |
| description | Piper
betle
leaves have traditionally been used to treat many diseases, including bacterial infections. The present study aimed to investigate the antibacterial, antibiofilm, and anti-adhesion activities of
P.
betle
extract against avian pathogenic
Escherichia
coli
(APEC). The ethanol extract of
P.
betle
leaves demonstrated strong antibacterial activity against clinical isolates of APEC with MIC and MBC values ranging from 0.5 to 1.0 mg/mL as compared with 1% DMSO, a negative control. Disruption and breakdown of the bacterial cells were detected when the cells were challenged with the extract at 2 × MIC. Bacterial cells treated with the extract demonstrated longer cells without a septum, compared to the control. The extract at 1/8, 1/4, and 1/2 × MIC significantly inhibited the formation of the bacterial biofilm of all the tested isolates except the isolate CH10 (
P
|
| doi_str_mv | 10.1007/s00203-021-02701-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_sprin</sourceid><recordid>TN_cdi_proquest_journals_2612223459</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2612223459</sourcerecordid><originalsourceid>FETCH-LOGICAL-p157t-60444e9e65ea0f0acca587a5d693d8474d66d5d0c313d01d00ad092e35a48e203</originalsourceid><addsrcrecordid>eNpFkMtKA0EQRRtRMEZ_wFWDW1urH_NahhAfENCFgruhMl2TdBhnxulOkODH20kEF0Vxi0NdOIxdS7iTANm9B1CgBSgZJwMpdidsJI1WAjL1ccpGoEGJvND6nF14vwaQKs_zEfuZtMEtsAo0OGxuOe6j62rXfO6DPRwE2hV517U8gm7rgiPPu5q_up6GBYWGeENYc_oOQyQ4LtG1PvDJ1mHLewyrbkmtq_jMV6tYVK0cVl3jLtlZjY2nq789Zu8Ps7fpk5i_PD5PJ3PRyyQLIgVjDBWUJoRQA1YVJnmGiU0LbXOTGZumNrFQaaktSAuAFgpFOkGTU_QyZjfHv_3QfW3Ih3LdbYY2VpYqlUopbZIiUvpI-X5w7ZKGf0pCuddcHjWXUXN50Fzu9C8bMnJ3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2612223459</pqid></control><display><type>article</type><title>Antibacterial, antibiofilm, and anti-adhesion activities of Piperbetle leaf extract against Avian pathogenic Escherichiacoli</title><source>SpringerLink Journals - AutoHoldings</source><creator>Kulnanan, Pawinee ; Chuprom, Julalak ; Thomrongsuwannakij, Thotsapol ; Romyasamit, Chonticha ; Sangkanu, Suthinee ; Manin, Nannaphat ; Nissapatorn, Veeranoot ; de Lourdes Pereira, Maria ; Wilairatana, Polrat ; Kitpipit, Warangkana ; Mitsuwan, Watcharapong</creator><creatorcontrib>Kulnanan, Pawinee ; Chuprom, Julalak ; Thomrongsuwannakij, Thotsapol ; Romyasamit, Chonticha ; Sangkanu, Suthinee ; Manin, Nannaphat ; Nissapatorn, Veeranoot ; de Lourdes Pereira, Maria ; Wilairatana, Polrat ; Kitpipit, Warangkana ; Mitsuwan, Watcharapong</creatorcontrib><description>Piper
betle
leaves have traditionally been used to treat many diseases, including bacterial infections. The present study aimed to investigate the antibacterial, antibiofilm, and anti-adhesion activities of
P.
betle
extract against avian pathogenic
Escherichia
coli
(APEC). The ethanol extract of
P.
betle
leaves demonstrated strong antibacterial activity against clinical isolates of APEC with MIC and MBC values ranging from 0.5 to 1.0 mg/mL as compared with 1% DMSO, a negative control. Disruption and breakdown of the bacterial cells were detected when the cells were challenged with the extract at 2 × MIC. Bacterial cells treated with the extract demonstrated longer cells without a septum, compared to the control. The extract at 1/8, 1/4, and 1/2 × MIC significantly inhibited the formation of the bacterial biofilm of all the tested isolates except the isolate CH10 (
P
<
0.05
) without inhibiting growth. At 1/2 × MIC, 55% of the biofilm inhibition was detected in APEC CH09, a strong biofilm producer. At 32 × MIC, 88% of the inhibition of viable cells embedded in the mature biofilm was detected in APEC CH09. Reduction in the bacterial adhesion to surfaces was shown when APEC were treated with sub-MICs of the extract as observed by SEM. Hydroxychavicol was found to be the major compound presented in the leaf extract as detected by GC–MS analysis. The information suggested potential medicinal benefits of
P.
betle
extract to inhibit the growth, biofilm, and adhesion of avian pathogenic
E.
coli
.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-021-02701-z</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adhesion ; Antibacterial activity ; Bacteria ; Bacterial diseases ; Biochemistry ; Biofilms ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Clinical isolates ; E coli ; Ecology ; Ethanol ; Leaves ; Life Sciences ; Microbial Ecology ; Microbiology ; Minimum inhibitory concentration ; Original Paper ; Plant extracts ; Septum</subject><ispartof>Archives of microbiology, 2022, Vol.204 (1)</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p157t-60444e9e65ea0f0acca587a5d693d8474d66d5d0c313d01d00ad092e35a48e203</cites><orcidid>0000-0002-5257-4626</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-021-02701-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-021-02701-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Kulnanan, Pawinee</creatorcontrib><creatorcontrib>Chuprom, Julalak</creatorcontrib><creatorcontrib>Thomrongsuwannakij, Thotsapol</creatorcontrib><creatorcontrib>Romyasamit, Chonticha</creatorcontrib><creatorcontrib>Sangkanu, Suthinee</creatorcontrib><creatorcontrib>Manin, Nannaphat</creatorcontrib><creatorcontrib>Nissapatorn, Veeranoot</creatorcontrib><creatorcontrib>de Lourdes Pereira, Maria</creatorcontrib><creatorcontrib>Wilairatana, Polrat</creatorcontrib><creatorcontrib>Kitpipit, Warangkana</creatorcontrib><creatorcontrib>Mitsuwan, Watcharapong</creatorcontrib><title>Antibacterial, antibiofilm, and anti-adhesion activities of Piperbetle leaf extract against Avian pathogenic Escherichiacoli</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><description>Piper
betle
leaves have traditionally been used to treat many diseases, including bacterial infections. The present study aimed to investigate the antibacterial, antibiofilm, and anti-adhesion activities of
P.
betle
extract against avian pathogenic
Escherichia
coli
(APEC). The ethanol extract of
P.
betle
leaves demonstrated strong antibacterial activity against clinical isolates of APEC with MIC and MBC values ranging from 0.5 to 1.0 mg/mL as compared with 1% DMSO, a negative control. Disruption and breakdown of the bacterial cells were detected when the cells were challenged with the extract at 2 × MIC. Bacterial cells treated with the extract demonstrated longer cells without a septum, compared to the control. The extract at 1/8, 1/4, and 1/2 × MIC significantly inhibited the formation of the bacterial biofilm of all the tested isolates except the isolate CH10 (
P
<
0.05
) without inhibiting growth. At 1/2 × MIC, 55% of the biofilm inhibition was detected in APEC CH09, a strong biofilm producer. At 32 × MIC, 88% of the inhibition of viable cells embedded in the mature biofilm was detected in APEC CH09. Reduction in the bacterial adhesion to surfaces was shown when APEC were treated with sub-MICs of the extract as observed by SEM. Hydroxychavicol was found to be the major compound presented in the leaf extract as detected by GC–MS analysis. The information suggested potential medicinal benefits of
P.
betle
extract to inhibit the growth, biofilm, and adhesion of avian pathogenic
E.
coli
.</description><subject>Adhesion</subject><subject>Antibacterial activity</subject><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>Biochemistry</subject><subject>Biofilms</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Clinical isolates</subject><subject>E coli</subject><subject>Ecology</subject><subject>Ethanol</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Minimum inhibitory concentration</subject><subject>Original Paper</subject><subject>Plant extracts</subject><subject>Septum</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpFkMtKA0EQRRtRMEZ_wFWDW1urH_NahhAfENCFgruhMl2TdBhnxulOkODH20kEF0Vxi0NdOIxdS7iTANm9B1CgBSgZJwMpdidsJI1WAjL1ccpGoEGJvND6nF14vwaQKs_zEfuZtMEtsAo0OGxuOe6j62rXfO6DPRwE2hV517U8gm7rgiPPu5q_up6GBYWGeENYc_oOQyQ4LtG1PvDJ1mHLewyrbkmtq_jMV6tYVK0cVl3jLtlZjY2nq789Zu8Ps7fpk5i_PD5PJ3PRyyQLIgVjDBWUJoRQA1YVJnmGiU0LbXOTGZumNrFQaaktSAuAFgpFOkGTU_QyZjfHv_3QfW3Ih3LdbYY2VpYqlUopbZIiUvpI-X5w7ZKGf0pCuddcHjWXUXN50Fzu9C8bMnJ3</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Kulnanan, Pawinee</creator><creator>Chuprom, Julalak</creator><creator>Thomrongsuwannakij, Thotsapol</creator><creator>Romyasamit, Chonticha</creator><creator>Sangkanu, Suthinee</creator><creator>Manin, Nannaphat</creator><creator>Nissapatorn, Veeranoot</creator><creator>de Lourdes Pereira, Maria</creator><creator>Wilairatana, Polrat</creator><creator>Kitpipit, Warangkana</creator><creator>Mitsuwan, Watcharapong</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-5257-4626</orcidid></search><sort><creationdate>2022</creationdate><title>Antibacterial, antibiofilm, and anti-adhesion activities of Piperbetle leaf extract against Avian pathogenic Escherichiacoli</title><author>Kulnanan, Pawinee ; Chuprom, Julalak ; Thomrongsuwannakij, Thotsapol ; Romyasamit, Chonticha ; Sangkanu, Suthinee ; Manin, Nannaphat ; Nissapatorn, Veeranoot ; de Lourdes Pereira, Maria ; Wilairatana, Polrat ; Kitpipit, Warangkana ; Mitsuwan, Watcharapong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p157t-60444e9e65ea0f0acca587a5d693d8474d66d5d0c313d01d00ad092e35a48e203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adhesion</topic><topic>Antibacterial activity</topic><topic>Bacteria</topic><topic>Bacterial diseases</topic><topic>Biochemistry</topic><topic>Biofilms</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Clinical isolates</topic><topic>E coli</topic><topic>Ecology</topic><topic>Ethanol</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Minimum inhibitory concentration</topic><topic>Original Paper</topic><topic>Plant extracts</topic><topic>Septum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kulnanan, Pawinee</creatorcontrib><creatorcontrib>Chuprom, Julalak</creatorcontrib><creatorcontrib>Thomrongsuwannakij, Thotsapol</creatorcontrib><creatorcontrib>Romyasamit, Chonticha</creatorcontrib><creatorcontrib>Sangkanu, Suthinee</creatorcontrib><creatorcontrib>Manin, Nannaphat</creatorcontrib><creatorcontrib>Nissapatorn, Veeranoot</creatorcontrib><creatorcontrib>de Lourdes Pereira, Maria</creatorcontrib><creatorcontrib>Wilairatana, Polrat</creatorcontrib><creatorcontrib>Kitpipit, Warangkana</creatorcontrib><creatorcontrib>Mitsuwan, Watcharapong</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kulnanan, Pawinee</au><au>Chuprom, Julalak</au><au>Thomrongsuwannakij, Thotsapol</au><au>Romyasamit, Chonticha</au><au>Sangkanu, Suthinee</au><au>Manin, Nannaphat</au><au>Nissapatorn, Veeranoot</au><au>de Lourdes Pereira, Maria</au><au>Wilairatana, Polrat</au><au>Kitpipit, Warangkana</au><au>Mitsuwan, Watcharapong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial, antibiofilm, and anti-adhesion activities of Piperbetle leaf extract against Avian pathogenic Escherichiacoli</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><date>2022</date><risdate>2022</risdate><volume>204</volume><issue>1</issue><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Piper
betle
leaves have traditionally been used to treat many diseases, including bacterial infections. The present study aimed to investigate the antibacterial, antibiofilm, and anti-adhesion activities of
P.
betle
extract against avian pathogenic
Escherichia
coli
(APEC). The ethanol extract of
P.
betle
leaves demonstrated strong antibacterial activity against clinical isolates of APEC with MIC and MBC values ranging from 0.5 to 1.0 mg/mL as compared with 1% DMSO, a negative control. Disruption and breakdown of the bacterial cells were detected when the cells were challenged with the extract at 2 × MIC. Bacterial cells treated with the extract demonstrated longer cells without a septum, compared to the control. The extract at 1/8, 1/4, and 1/2 × MIC significantly inhibited the formation of the bacterial biofilm of all the tested isolates except the isolate CH10 (
P
<
0.05
) without inhibiting growth. At 1/2 × MIC, 55% of the biofilm inhibition was detected in APEC CH09, a strong biofilm producer. At 32 × MIC, 88% of the inhibition of viable cells embedded in the mature biofilm was detected in APEC CH09. Reduction in the bacterial adhesion to surfaces was shown when APEC were treated with sub-MICs of the extract as observed by SEM. Hydroxychavicol was found to be the major compound presented in the leaf extract as detected by GC–MS analysis. The information suggested potential medicinal benefits of
P.
betle
extract to inhibit the growth, biofilm, and adhesion of avian pathogenic
E.
coli
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00203-021-02701-z</doi><orcidid>https://orcid.org/0000-0002-5257-4626</orcidid></addata></record> |
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| ispartof | Archives of microbiology, 2022, Vol.204 (1) |
| issn | 0302-8933 1432-072X |
| language | eng |
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| subjects | Adhesion Antibacterial activity Bacteria Bacterial diseases Biochemistry Biofilms Biomedical and Life Sciences Biotechnology Cell Biology Clinical isolates E coli Ecology Ethanol Leaves Life Sciences Microbial Ecology Microbiology Minimum inhibitory concentration Original Paper Plant extracts Septum |
| title | Antibacterial, antibiofilm, and anti-adhesion activities of Piperbetle leaf extract against Avian pathogenic Escherichiacoli |
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