Reconstruction of Hyper‐Thermostable Ancestral L‐Amino Acid Oxidase to Perform Deracemization to D‐Amino Acids

L‐amino acid oxidases (LAAOs) with broad substrate specificity can be used in the deracemization of D,L‐amino acids (D,L‐AAs) to their D‐enantiomers. Hyper‐thermostable LAAO (HTAncLAAO) was designed through a combination of manual sequence data mining and ancestral sequence reconstruction. Soluble e...

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Veröffentlicht in:	ChemCatChem 2021-12, Vol.13 (24), p.5228-5235
Hauptverfasser:	Ishida, Chiharu, Miyata, Ryo, Hasebe, Fumihito, Miyata, Azusa, Kumazawa, Shigenori, Ito, Sohei, Nakano, Shogo
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acid oxidase Amino acids ancestral sequence reconstruction biocatalysis Data mining deracemization E coli Enantiomers hyper thermostability L-amino acid oxidase Reconstruction Substrates Thermal stability
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