Silica/OCP Affects the Viability of Osteoblasts through ROS-Induced Autophagy
Objective. To explore the effects of silicone gel nanoparticles modified with octacalcium phosphate on the surface (silica/OCP) polymer drugs on the proliferation of osteoblasts and autophagy. Method. Silica/OCP was prepared in vitro, and the quality of the sample preparation was tested through char...
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| description | Objective. To explore the effects of silicone gel nanoparticles modified with octacalcium phosphate on the surface (silica/OCP) polymer drugs on the proliferation of osteoblasts and autophagy. Method. Silica/OCP was prepared in vitro, and the quality of the sample preparation was tested through characterization experiments. The osteoblast cell line (hFOB1.19) was treated with silica/OCP, autophagy inhibitor (3-methyladenine (3-MA)), and silica/OCP+3-MA, respectively. The proliferation of hFOB1.19 cells was detected through the methylthiazolyldiphenyl-tetrazolium bromide (MTT) kit. Flow cytometry was used to detect the cell apoptosis. The change in protein beclin1 and P62 expression in hFOB1.19 cells was observed in Western blot. An ROS detection kit was used to detect the content of reactive oxygen species in hFOB1.19 cells. Results. Silica/OCP was a sphere with a particle size of 50 nm to 130 nm and had an OCP phase in electron projection microscopy and X-ray diffraction techniques. The results indicated that OCP successfully modified silica and the material was successfully prepared. An MTT kit and flow cytometry test showed that the cell viability of the cells treated with silica/OCP increased significantly (P |
| doi_str_mv | 10.1155/2021/3159848 |
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To explore the effects of silicone gel nanoparticles modified with octacalcium phosphate on the surface (silica/OCP) polymer drugs on the proliferation of osteoblasts and autophagy. Method. Silica/OCP was prepared in vitro, and the quality of the sample preparation was tested through characterization experiments. The osteoblast cell line (hFOB1.19) was treated with silica/OCP, autophagy inhibitor (3-methyladenine (3-MA)), and silica/OCP+3-MA, respectively. The proliferation of hFOB1.19 cells was detected through the methylthiazolyldiphenyl-tetrazolium bromide (MTT) kit. Flow cytometry was used to detect the cell apoptosis. The change in protein beclin1 and P62 expression in hFOB1.19 cells was observed in Western blot. An ROS detection kit was used to detect the content of reactive oxygen species in hFOB1.19 cells. Results. Silica/OCP was a sphere with a particle size of 50 nm to 130 nm and had an OCP phase in electron projection microscopy and X-ray diffraction techniques. The results indicated that OCP successfully modified silica and the material was successfully prepared. An MTT kit and flow cytometry test showed that the cell viability of the cells treated with silica/OCP increased significantly (P<0.05), and the intracellular apoptosis phenomenon was significantly decreased (P<0.05) compared to the control group. Moreover, the inhibition of cell viability and promotion of apoptosis caused by the autophagy inhibitor 3-MA can be rescued. Western blotting demonstrated that the protein level of beclin1 in osteoblasts reached the highest after six hours of treatment with silica/OCP, and the protein level of p62, the substrate protein of autophagy, reached the lowest. At the same time, treatment of cells with the autophagy inhibitor 3-MA and silica/OCP+3-MA found that the protein levels of beclin1 and p62 in the silica/OCP+3-MA group were adjusted back compared to the 3-MA group. After adding the autophagy inhibitor, the reactive oxygen content in the cell was significantly increased (P<0.05) in the silica/OCP group. In the presence of intracellular reactive oxygen inhibitors catalase and silica/OCP, the cell viability of osteoblasts was significantly lower than that of the silica/OCP group but significantly higher than that of the silica/OCP+3-MA group. The apoptosis level of the silica/OCP+catalase group was also significantly lower than that of the silica/OCP+3-MA group (P<0.05) but was significantly higher than that of the silica/OCP group (P<0.05). Conclusion. Silica/OCP nanoparticles can upregulate the level of autophagy in osteoblasts and promote the proliferation of osteoblasts.</description><identifier>ISSN: 1687-4110</identifier><identifier>EISSN: 1687-4129</identifier><identifier>DOI: 10.1155/2021/3159848</identifier><language>eng</language><publisher>LONDON: Hindawi</publisher><subject>Apoptosis ; Autophagy ; Biomedical materials ; Calcium phosphates ; Catalase ; Cell culture ; Flow cytometry ; Inhibitors ; Materials Science ; Materials Science, Multidisciplinary ; Metabolism ; Nanomaterials ; Nanoparticles ; Nanoscience & Nanotechnology ; Osteoblasts ; Osteoporosis ; Oxidative stress ; Oxygen ; Oxygen content ; Proteins ; Science & Technology ; Science & Technology - Other Topics ; Silicon dioxide ; Substrates ; Technology</subject><ispartof>Journal of nanomaterials, 2021, Vol.2021, p.1-9, Article 3159848</ispartof><rights>Copyright © 2021 Jianghao Gong et al.</rights><rights>Copyright © 2021 Jianghao Gong et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>0</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000767523000002</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c337t-cd19807909a4522b0722e3b9faf851aa4eaa99cc451b8db7ad3c39166d6327433</citedby><cites>FETCH-LOGICAL-c337t-cd19807909a4522b0722e3b9faf851aa4eaa99cc451b8db7ad3c39166d6327433</cites><orcidid>0000-0002-1929-0015</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930,39263</link.rule.ids></links><search><contributor>Owais, Mohammad</contributor><contributor>Mohammad Owais</contributor><creatorcontrib>Gong, Jianghao</creatorcontrib><creatorcontrib>Fu, Shangjun</creatorcontrib><creatorcontrib>Zhou, Zhenghao</creatorcontrib><title>Silica/OCP Affects the Viability of Osteoblasts through ROS-Induced Autophagy</title><title>Journal of nanomaterials</title><addtitle>J NANOMATER</addtitle><description>Objective. To explore the effects of silicone gel nanoparticles modified with octacalcium phosphate on the surface (silica/OCP) polymer drugs on the proliferation of osteoblasts and autophagy. Method. Silica/OCP was prepared in vitro, and the quality of the sample preparation was tested through characterization experiments. The osteoblast cell line (hFOB1.19) was treated with silica/OCP, autophagy inhibitor (3-methyladenine (3-MA)), and silica/OCP+3-MA, respectively. The proliferation of hFOB1.19 cells was detected through the methylthiazolyldiphenyl-tetrazolium bromide (MTT) kit. Flow cytometry was used to detect the cell apoptosis. The change in protein beclin1 and P62 expression in hFOB1.19 cells was observed in Western blot. An ROS detection kit was used to detect the content of reactive oxygen species in hFOB1.19 cells. Results. Silica/OCP was a sphere with a particle size of 50 nm to 130 nm and had an OCP phase in electron projection microscopy and X-ray diffraction techniques. The results indicated that OCP successfully modified silica and the material was successfully prepared. An MTT kit and flow cytometry test showed that the cell viability of the cells treated with silica/OCP increased significantly (P<0.05), and the intracellular apoptosis phenomenon was significantly decreased (P<0.05) compared to the control group. Moreover, the inhibition of cell viability and promotion of apoptosis caused by the autophagy inhibitor 3-MA can be rescued. Western blotting demonstrated that the protein level of beclin1 in osteoblasts reached the highest after six hours of treatment with silica/OCP, and the protein level of p62, the substrate protein of autophagy, reached the lowest. At the same time, treatment of cells with the autophagy inhibitor 3-MA and silica/OCP+3-MA found that the protein levels of beclin1 and p62 in the silica/OCP+3-MA group were adjusted back compared to the 3-MA group. After adding the autophagy inhibitor, the reactive oxygen content in the cell was significantly increased (P<0.05) in the silica/OCP group. In the presence of intracellular reactive oxygen inhibitors catalase and silica/OCP, the cell viability of osteoblasts was significantly lower than that of the silica/OCP group but significantly higher than that of the silica/OCP+3-MA group. The apoptosis level of the silica/OCP+catalase group was also significantly lower than that of the silica/OCP+3-MA group (P<0.05) but was significantly higher than that of the silica/OCP group (P<0.05). Conclusion. Silica/OCP nanoparticles can upregulate the level of autophagy in osteoblasts and promote the proliferation of osteoblasts.</description><subject>Apoptosis</subject><subject>Autophagy</subject><subject>Biomedical materials</subject><subject>Calcium phosphates</subject><subject>Catalase</subject><subject>Cell culture</subject><subject>Flow cytometry</subject><subject>Inhibitors</subject><subject>Materials Science</subject><subject>Materials Science, Multidisciplinary</subject><subject>Metabolism</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Nanoscience & Nanotechnology</subject><subject>Osteoblasts</subject><subject>Osteoporosis</subject><subject>Oxidative stress</subject><subject>Oxygen</subject><subject>Oxygen content</subject><subject>Proteins</subject><subject>Science & Technology</subject><subject>Science & Technology - Other Topics</subject><subject>Silicon dioxide</subject><subject>Substrates</subject><subject>Technology</subject><issn>1687-4110</issn><issn>1687-4129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>HGBXW</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqNkF9LwzAUxYMoOKdvfoCCj1qXP02TPI7idDCpOPW1pGmyZsxmNilj397Ojfkm3pd74fzOvZcDwDWC9whROsIQoxFBVPCEn4ABSjmLE4TF6XFG8BxceL-EMKGC4gF4ntuVVXKUZy_R2Bitgo9CraMPK8teCdvImSj3QbtyJf2P2LpuUUev-TyeNlWndBWNu-DWtVxsL8GZkSuvrw59CN4nD2_ZUzzLH6fZeBYrQliIVYUEh0xAIROKcQkZxpqUwkjDKZIy0VIKoVRCUcmrksmKKCJQmlYpwSwhZAhu9nvXrfvqtA_F0nVt058scAoFokRw2lN3e0q1zvtWm2Ld2k_ZbgsEi11gxS6w4hBYj_M9vtGlM15Z3Sh9tEAIWcooJnBXOLNBBuuazHVN6K23_7f-0rVtKrmxf7_1DbJAiYQ</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Gong, Jianghao</creator><creator>Fu, Shangjun</creator><creator>Zhou, Zhenghao</creator><general>Hindawi</general><general>Hindawi Publishing Group</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>L7M</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0002-1929-0015</orcidid></search><sort><creationdate>2021</creationdate><title>Silica/OCP Affects the Viability of Osteoblasts through ROS-Induced Autophagy</title><author>Gong, Jianghao ; Fu, Shangjun ; Zhou, Zhenghao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-cd19807909a4522b0722e3b9faf851aa4eaa99cc451b8db7ad3c39166d6327433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Apoptosis</topic><topic>Autophagy</topic><topic>Biomedical materials</topic><topic>Calcium phosphates</topic><topic>Catalase</topic><topic>Cell culture</topic><topic>Flow cytometry</topic><topic>Inhibitors</topic><topic>Materials Science</topic><topic>Materials Science, Multidisciplinary</topic><topic>Metabolism</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Nanoscience & Nanotechnology</topic><topic>Osteoblasts</topic><topic>Osteoporosis</topic><topic>Oxidative stress</topic><topic>Oxygen</topic><topic>Oxygen content</topic><topic>Proteins</topic><topic>Science & Technology</topic><topic>Science & Technology - Other Topics</topic><topic>Silicon dioxide</topic><topic>Substrates</topic><topic>Technology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Jianghao</creatorcontrib><creatorcontrib>Fu, Shangjun</creatorcontrib><creatorcontrib>Zhou, Zhenghao</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of nanomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Jianghao</au><au>Fu, Shangjun</au><au>Zhou, Zhenghao</au><au>Owais, Mohammad</au><au>Mohammad Owais</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silica/OCP Affects the Viability of Osteoblasts through ROS-Induced Autophagy</atitle><jtitle>Journal of nanomaterials</jtitle><stitle>J NANOMATER</stitle><date>2021</date><risdate>2021</risdate><volume>2021</volume><spage>1</spage><epage>9</epage><pages>1-9</pages><artnum>3159848</artnum><issn>1687-4110</issn><eissn>1687-4129</eissn><abstract>Objective. To explore the effects of silicone gel nanoparticles modified with octacalcium phosphate on the surface (silica/OCP) polymer drugs on the proliferation of osteoblasts and autophagy. Method. Silica/OCP was prepared in vitro, and the quality of the sample preparation was tested through characterization experiments. The osteoblast cell line (hFOB1.19) was treated with silica/OCP, autophagy inhibitor (3-methyladenine (3-MA)), and silica/OCP+3-MA, respectively. The proliferation of hFOB1.19 cells was detected through the methylthiazolyldiphenyl-tetrazolium bromide (MTT) kit. Flow cytometry was used to detect the cell apoptosis. The change in protein beclin1 and P62 expression in hFOB1.19 cells was observed in Western blot. An ROS detection kit was used to detect the content of reactive oxygen species in hFOB1.19 cells. Results. Silica/OCP was a sphere with a particle size of 50 nm to 130 nm and had an OCP phase in electron projection microscopy and X-ray diffraction techniques. The results indicated that OCP successfully modified silica and the material was successfully prepared. An MTT kit and flow cytometry test showed that the cell viability of the cells treated with silica/OCP increased significantly (P<0.05), and the intracellular apoptosis phenomenon was significantly decreased (P<0.05) compared to the control group. Moreover, the inhibition of cell viability and promotion of apoptosis caused by the autophagy inhibitor 3-MA can be rescued. Western blotting demonstrated that the protein level of beclin1 in osteoblasts reached the highest after six hours of treatment with silica/OCP, and the protein level of p62, the substrate protein of autophagy, reached the lowest. At the same time, treatment of cells with the autophagy inhibitor 3-MA and silica/OCP+3-MA found that the protein levels of beclin1 and p62 in the silica/OCP+3-MA group were adjusted back compared to the 3-MA group. After adding the autophagy inhibitor, the reactive oxygen content in the cell was significantly increased (P<0.05) in the silica/OCP group. In the presence of intracellular reactive oxygen inhibitors catalase and silica/OCP, the cell viability of osteoblasts was significantly lower than that of the silica/OCP group but significantly higher than that of the silica/OCP+3-MA group. The apoptosis level of the silica/OCP+catalase group was also significantly lower than that of the silica/OCP+3-MA group (P<0.05) but was significantly higher than that of the silica/OCP group (P<0.05). Conclusion. Silica/OCP nanoparticles can upregulate the level of autophagy in osteoblasts and promote the proliferation of osteoblasts.</abstract><cop>LONDON</cop><pub>Hindawi</pub><doi>10.1155/2021/3159848</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-1929-0015</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Autophagy Biomedical materials Calcium phosphates Catalase Cell culture Flow cytometry Inhibitors Materials Science Materials Science, Multidisciplinary Metabolism Nanomaterials Nanoparticles Nanoscience & Nanotechnology Osteoblasts Osteoporosis Oxidative stress Oxygen Oxygen content Proteins Science & Technology Science & Technology - Other Topics Silicon dioxide Substrates Technology |
| title | Silica/OCP Affects the Viability of Osteoblasts through ROS-Induced Autophagy |
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