Plasma l‐indospicine and 3‐nitropropionic acid in ponies fed creeping indigo: Comparison with results from an episode of presumptive creeping indigo toxicosis
Background Creeping indigo (Indigofera spicata) toxicosis is an emerging problem among horses in Florida and bordering states. Objectives To quantify the putative toxins l‐indospicine (IND) and 3‐nitropropionic acid (NPA) in creeping indigo collected from multiple sites and to measure plasma toxin c...
Gespeichert in:
| Veröffentlicht in: | Equine veterinary journal 2022-01, Vol.54 (1), p.145-152 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 152 |
|---|---|
| container_issue | 1 |
| container_start_page | 145 |
| container_title | Equine veterinary journal |
| container_volume | 54 |
| creator | Darby, Shannon Sanchez, L. Chris Mallicote, Martha F. House, Amanda M. Plummer, Caryn E. Nadruz, Veridiana Benmoha, Rachel H. Roberts, Stephen M. Derendorf, Hartmut Silva‐Sanchez, Cecilia Claire, Jami MacKay, Robert J. |
| description | Background
Creeping indigo (Indigofera spicata) toxicosis is an emerging problem among horses in Florida and bordering states.
Objectives
To quantify the putative toxins l‐indospicine (IND) and 3‐nitropropionic acid (NPA) in creeping indigo collected from multiple sites and to measure plasma toxin concentrations in ponies fed creeping indigo and horses with presumptive creeping indigo toxicosis.
Study design
Experimental descriptive study with descriptive observational field investigation.
Methods
Air‐dried creeping indigo was assayed for IND and NPA content. Five ponies were fed chopped creeping indigo containing 1 mg/kg/day of IND and trace amounts of NPA for 5 days, then observed for 28 days. Blood samples from these ponies and from horses involved in a presumptive creeping indigo toxicosis were assayed for IND and NPA.
Results
IND in creeping indigo plants was 0.4‐3.5 mg/g dry matter whereas NPA was |
| doi_str_mv | 10.1111/evj.13415 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2607488155</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2607488155</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3535-ddd10e559a4e26ff9fb92a5f1ba9354ae3e7e098e78fb99acaf3897a3c9a3b2e3</originalsourceid><addsrcrecordid>eNp1kcFuFSEUhomxsbfVhS9gSFy5mBbmDDODO3NTq6aJLtTthAuHem5mYIS5rd35CD5DH80nkXqrCxMJCXDOl4_Az9hTKU5kGad4tT2R0Ej1gK1q0dQVgGgfslXZqkq2TXPIjnLeCgFQN_UjdggAre7absVuP4wmT4aPP7__oOBinslSQG6C41BqgZYU5zIpBrLcWHKcAp_LCTP36LhNiDOFy1J2dBlf8nWcZpMox8CvafnCE-bduBQ4xal4eaFzdMij5_Ndb5oXusJ_PXyJ38jGTPkxO_BmzPjkfj1mn16ffVy_qS7en79dv7qoLChQlXNOClRKmwbr1nvtN7o2ysuN0aAag4AdCt1j15eONtZ46HVnwGoDmxrhmD3fe8tzv-4wL8M27lIoVw51K7qm76VShXqxp2yKOSf0w5xoMulmkGK4S2MoaQy_0yjss3vjbjOh-0v--f4CnO6Baxrx5v-m4ezzu73yF0i1mlQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2607488155</pqid></control><display><type>article</type><title>Plasma l‐indospicine and 3‐nitropropionic acid in ponies fed creeping indigo: Comparison with results from an episode of presumptive creeping indigo toxicosis</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Darby, Shannon ; Sanchez, L. Chris ; Mallicote, Martha F. ; House, Amanda M. ; Plummer, Caryn E. ; Nadruz, Veridiana ; Benmoha, Rachel H. ; Roberts, Stephen M. ; Derendorf, Hartmut ; Silva‐Sanchez, Cecilia ; Claire, Jami ; MacKay, Robert J.</creator><creatorcontrib>Darby, Shannon ; Sanchez, L. Chris ; Mallicote, Martha F. ; House, Amanda M. ; Plummer, Caryn E. ; Nadruz, Veridiana ; Benmoha, Rachel H. ; Roberts, Stephen M. ; Derendorf, Hartmut ; Silva‐Sanchez, Cecilia ; Claire, Jami ; MacKay, Robert J.</creatorcontrib><description>Background
Creeping indigo (Indigofera spicata) toxicosis is an emerging problem among horses in Florida and bordering states.
Objectives
To quantify the putative toxins l‐indospicine (IND) and 3‐nitropropionic acid (NPA) in creeping indigo collected from multiple sites and to measure plasma toxin concentrations in ponies fed creeping indigo and horses with presumptive creeping indigo toxicosis.
Study design
Experimental descriptive study with descriptive observational field investigation.
Methods
Air‐dried creeping indigo was assayed for IND and NPA content. Five ponies were fed chopped creeping indigo containing 1 mg/kg/day of IND and trace amounts of NPA for 5 days, then observed for 28 days. Blood samples from these ponies and from horses involved in a presumptive creeping indigo toxicosis were assayed for IND and NPA.
Results
IND in creeping indigo plants was 0.4‐3.5 mg/g dry matter whereas NPA was <0.01 to 0.03 mg/g. During creeping indigo feeding, clinical and laboratory signs were unchanged except for significant weight loss (median 6%, range 2%‐9%; p = .04) and significant increase from baseline plasma protein concentration (median 16 g/L, range 8‐25 g/L; p < .001). These changes could not definitively be ascribed to creeping indigo ingestion. Plasma IND rose to 3.9 ± 0.52 mg/L on day 6. Pharmacokinetic modelling indicated an elimination half‐life of 25 days and a steady state plasma concentration of 22 mg/L. Plasma IND concentration in sick horses during an incident of creeping indigo toxicosis was approximately twice that of clinically normal pasture mates. Plasma NPA was <0.05 mg/L in all samples.
Main limitations
Creeping indigo used in the feeding trial may not be representative of plants involved in creeping indigo toxicosis. There was no control group without creeping indigo in the feeding trial.
Conclusions
Indospicine can be detected in blood of horses consuming creeping indigo and the toxin accumulates in tissues and clears slowly. The role of NPA in the neurological signs of this syndrome is unclear.</description><identifier>ISSN: 0425-1644</identifier><identifier>EISSN: 2042-3306</identifier><identifier>DOI: 10.1111/evj.13415</identifier><identifier>PMID: 33369767</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>3‐nitropropionic acid ; Animals ; creeping indigo ; horse ; Horse Diseases - chemically induced ; Horses ; Indigo Carmine ; Indigofera ; l‐indospicine ; Nitro Compounds ; Norleucine - analogs & derivatives ; Plasma ; Propionates ; toxicosis</subject><ispartof>Equine veterinary journal, 2022-01, Vol.54 (1), p.145-152</ispartof><rights>2020 EVJ Ltd</rights><rights>2020 EVJ Ltd.</rights><rights>Copyright © 2022 EVJ Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-ddd10e559a4e26ff9fb92a5f1ba9354ae3e7e098e78fb99acaf3897a3c9a3b2e3</citedby><cites>FETCH-LOGICAL-c3535-ddd10e559a4e26ff9fb92a5f1ba9354ae3e7e098e78fb99acaf3897a3c9a3b2e3</cites><orcidid>0000-0002-4450-9859 ; 0000-0002-6404-5828 ; 0000-0002-5432-302X ; 0000-0001-7362-5325 ; 0000-0002-1785-2459</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fevj.13415$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fevj.13415$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33369767$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Darby, Shannon</creatorcontrib><creatorcontrib>Sanchez, L. Chris</creatorcontrib><creatorcontrib>Mallicote, Martha F.</creatorcontrib><creatorcontrib>House, Amanda M.</creatorcontrib><creatorcontrib>Plummer, Caryn E.</creatorcontrib><creatorcontrib>Nadruz, Veridiana</creatorcontrib><creatorcontrib>Benmoha, Rachel H.</creatorcontrib><creatorcontrib>Roberts, Stephen M.</creatorcontrib><creatorcontrib>Derendorf, Hartmut</creatorcontrib><creatorcontrib>Silva‐Sanchez, Cecilia</creatorcontrib><creatorcontrib>Claire, Jami</creatorcontrib><creatorcontrib>MacKay, Robert J.</creatorcontrib><title>Plasma l‐indospicine and 3‐nitropropionic acid in ponies fed creeping indigo: Comparison with results from an episode of presumptive creeping indigo toxicosis</title><title>Equine veterinary journal</title><addtitle>Equine Vet J</addtitle><description>Background
Creeping indigo (Indigofera spicata) toxicosis is an emerging problem among horses in Florida and bordering states.
Objectives
To quantify the putative toxins l‐indospicine (IND) and 3‐nitropropionic acid (NPA) in creeping indigo collected from multiple sites and to measure plasma toxin concentrations in ponies fed creeping indigo and horses with presumptive creeping indigo toxicosis.
Study design
Experimental descriptive study with descriptive observational field investigation.
Methods
Air‐dried creeping indigo was assayed for IND and NPA content. Five ponies were fed chopped creeping indigo containing 1 mg/kg/day of IND and trace amounts of NPA for 5 days, then observed for 28 days. Blood samples from these ponies and from horses involved in a presumptive creeping indigo toxicosis were assayed for IND and NPA.
Results
IND in creeping indigo plants was 0.4‐3.5 mg/g dry matter whereas NPA was <0.01 to 0.03 mg/g. During creeping indigo feeding, clinical and laboratory signs were unchanged except for significant weight loss (median 6%, range 2%‐9%; p = .04) and significant increase from baseline plasma protein concentration (median 16 g/L, range 8‐25 g/L; p < .001). These changes could not definitively be ascribed to creeping indigo ingestion. Plasma IND rose to 3.9 ± 0.52 mg/L on day 6. Pharmacokinetic modelling indicated an elimination half‐life of 25 days and a steady state plasma concentration of 22 mg/L. Plasma IND concentration in sick horses during an incident of creeping indigo toxicosis was approximately twice that of clinically normal pasture mates. Plasma NPA was <0.05 mg/L in all samples.
Main limitations
Creeping indigo used in the feeding trial may not be representative of plants involved in creeping indigo toxicosis. There was no control group without creeping indigo in the feeding trial.
Conclusions
Indospicine can be detected in blood of horses consuming creeping indigo and the toxin accumulates in tissues and clears slowly. The role of NPA in the neurological signs of this syndrome is unclear.</description><subject>3‐nitropropionic acid</subject><subject>Animals</subject><subject>creeping indigo</subject><subject>horse</subject><subject>Horse Diseases - chemically induced</subject><subject>Horses</subject><subject>Indigo Carmine</subject><subject>Indigofera</subject><subject>l‐indospicine</subject><subject>Nitro Compounds</subject><subject>Norleucine - analogs & derivatives</subject><subject>Plasma</subject><subject>Propionates</subject><subject>toxicosis</subject><issn>0425-1644</issn><issn>2042-3306</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFuFSEUhomxsbfVhS9gSFy5mBbmDDODO3NTq6aJLtTthAuHem5mYIS5rd35CD5DH80nkXqrCxMJCXDOl4_Az9hTKU5kGad4tT2R0Ej1gK1q0dQVgGgfslXZqkq2TXPIjnLeCgFQN_UjdggAre7absVuP4wmT4aPP7__oOBinslSQG6C41BqgZYU5zIpBrLcWHKcAp_LCTP36LhNiDOFy1J2dBlf8nWcZpMox8CvafnCE-bduBQ4xal4eaFzdMij5_Ndb5oXusJ_PXyJ38jGTPkxO_BmzPjkfj1mn16ffVy_qS7en79dv7qoLChQlXNOClRKmwbr1nvtN7o2ysuN0aAag4AdCt1j15eONtZ46HVnwGoDmxrhmD3fe8tzv-4wL8M27lIoVw51K7qm76VShXqxp2yKOSf0w5xoMulmkGK4S2MoaQy_0yjss3vjbjOh-0v--f4CnO6Baxrx5v-m4ezzu73yF0i1mlQ</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Darby, Shannon</creator><creator>Sanchez, L. Chris</creator><creator>Mallicote, Martha F.</creator><creator>House, Amanda M.</creator><creator>Plummer, Caryn E.</creator><creator>Nadruz, Veridiana</creator><creator>Benmoha, Rachel H.</creator><creator>Roberts, Stephen M.</creator><creator>Derendorf, Hartmut</creator><creator>Silva‐Sanchez, Cecilia</creator><creator>Claire, Jami</creator><creator>MacKay, Robert J.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0002-4450-9859</orcidid><orcidid>https://orcid.org/0000-0002-6404-5828</orcidid><orcidid>https://orcid.org/0000-0002-5432-302X</orcidid><orcidid>https://orcid.org/0000-0001-7362-5325</orcidid><orcidid>https://orcid.org/0000-0002-1785-2459</orcidid></search><sort><creationdate>202201</creationdate><title>Plasma l‐indospicine and 3‐nitropropionic acid in ponies fed creeping indigo: Comparison with results from an episode of presumptive creeping indigo toxicosis</title><author>Darby, Shannon ; Sanchez, L. Chris ; Mallicote, Martha F. ; House, Amanda M. ; Plummer, Caryn E. ; Nadruz, Veridiana ; Benmoha, Rachel H. ; Roberts, Stephen M. ; Derendorf, Hartmut ; Silva‐Sanchez, Cecilia ; Claire, Jami ; MacKay, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-ddd10e559a4e26ff9fb92a5f1ba9354ae3e7e098e78fb99acaf3897a3c9a3b2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>3‐nitropropionic acid</topic><topic>Animals</topic><topic>creeping indigo</topic><topic>horse</topic><topic>Horse Diseases - chemically induced</topic><topic>Horses</topic><topic>Indigo Carmine</topic><topic>Indigofera</topic><topic>l‐indospicine</topic><topic>Nitro Compounds</topic><topic>Norleucine - analogs & derivatives</topic><topic>Plasma</topic><topic>Propionates</topic><topic>toxicosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Darby, Shannon</creatorcontrib><creatorcontrib>Sanchez, L. Chris</creatorcontrib><creatorcontrib>Mallicote, Martha F.</creatorcontrib><creatorcontrib>House, Amanda M.</creatorcontrib><creatorcontrib>Plummer, Caryn E.</creatorcontrib><creatorcontrib>Nadruz, Veridiana</creatorcontrib><creatorcontrib>Benmoha, Rachel H.</creatorcontrib><creatorcontrib>Roberts, Stephen M.</creatorcontrib><creatorcontrib>Derendorf, Hartmut</creatorcontrib><creatorcontrib>Silva‐Sanchez, Cecilia</creatorcontrib><creatorcontrib>Claire, Jami</creatorcontrib><creatorcontrib>MacKay, Robert J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Equine veterinary journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Darby, Shannon</au><au>Sanchez, L. Chris</au><au>Mallicote, Martha F.</au><au>House, Amanda M.</au><au>Plummer, Caryn E.</au><au>Nadruz, Veridiana</au><au>Benmoha, Rachel H.</au><au>Roberts, Stephen M.</au><au>Derendorf, Hartmut</au><au>Silva‐Sanchez, Cecilia</au><au>Claire, Jami</au><au>MacKay, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasma l‐indospicine and 3‐nitropropionic acid in ponies fed creeping indigo: Comparison with results from an episode of presumptive creeping indigo toxicosis</atitle><jtitle>Equine veterinary journal</jtitle><addtitle>Equine Vet J</addtitle><date>2022-01</date><risdate>2022</risdate><volume>54</volume><issue>1</issue><spage>145</spage><epage>152</epage><pages>145-152</pages><issn>0425-1644</issn><eissn>2042-3306</eissn><abstract>Background
Creeping indigo (Indigofera spicata) toxicosis is an emerging problem among horses in Florida and bordering states.
Objectives
To quantify the putative toxins l‐indospicine (IND) and 3‐nitropropionic acid (NPA) in creeping indigo collected from multiple sites and to measure plasma toxin concentrations in ponies fed creeping indigo and horses with presumptive creeping indigo toxicosis.
Study design
Experimental descriptive study with descriptive observational field investigation.
Methods
Air‐dried creeping indigo was assayed for IND and NPA content. Five ponies were fed chopped creeping indigo containing 1 mg/kg/day of IND and trace amounts of NPA for 5 days, then observed for 28 days. Blood samples from these ponies and from horses involved in a presumptive creeping indigo toxicosis were assayed for IND and NPA.
Results
IND in creeping indigo plants was 0.4‐3.5 mg/g dry matter whereas NPA was <0.01 to 0.03 mg/g. During creeping indigo feeding, clinical and laboratory signs were unchanged except for significant weight loss (median 6%, range 2%‐9%; p = .04) and significant increase from baseline plasma protein concentration (median 16 g/L, range 8‐25 g/L; p < .001). These changes could not definitively be ascribed to creeping indigo ingestion. Plasma IND rose to 3.9 ± 0.52 mg/L on day 6. Pharmacokinetic modelling indicated an elimination half‐life of 25 days and a steady state plasma concentration of 22 mg/L. Plasma IND concentration in sick horses during an incident of creeping indigo toxicosis was approximately twice that of clinically normal pasture mates. Plasma NPA was <0.05 mg/L in all samples.
Main limitations
Creeping indigo used in the feeding trial may not be representative of plants involved in creeping indigo toxicosis. There was no control group without creeping indigo in the feeding trial.
Conclusions
Indospicine can be detected in blood of horses consuming creeping indigo and the toxin accumulates in tissues and clears slowly. The role of NPA in the neurological signs of this syndrome is unclear.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33369767</pmid><doi>10.1111/evj.13415</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4450-9859</orcidid><orcidid>https://orcid.org/0000-0002-6404-5828</orcidid><orcidid>https://orcid.org/0000-0002-5432-302X</orcidid><orcidid>https://orcid.org/0000-0001-7362-5325</orcidid><orcidid>https://orcid.org/0000-0002-1785-2459</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0425-1644 |
| ispartof | Equine veterinary journal, 2022-01, Vol.54 (1), p.145-152 |
| issn | 0425-1644 2042-3306 |
| language | eng |
| recordid | cdi_proquest_journals_2607488155 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 3‐nitropropionic acid Animals creeping indigo horse Horse Diseases - chemically induced Horses Indigo Carmine Indigofera l‐indospicine Nitro Compounds Norleucine - analogs & derivatives Plasma Propionates toxicosis |
| title | Plasma l‐indospicine and 3‐nitropropionic acid in ponies fed creeping indigo: Comparison with results from an episode of presumptive creeping indigo toxicosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T15%3A53%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasma%20l%E2%80%90indospicine%20and%203%E2%80%90nitropropionic%20acid%20in%20ponies%20fed%20creeping%20indigo:%20Comparison%20with%20results%20from%20an%20episode%20of%20presumptive%20creeping%20indigo%20toxicosis&rft.jtitle=Equine%20veterinary%20journal&rft.au=Darby,%20Shannon&rft.date=2022-01&rft.volume=54&rft.issue=1&rft.spage=145&rft.epage=152&rft.pages=145-152&rft.issn=0425-1644&rft.eissn=2042-3306&rft_id=info:doi/10.1111/evj.13415&rft_dat=%3Cproquest_cross%3E2607488155%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2607488155&rft_id=info:pmid/33369767&rfr_iscdi=true |