Sterilization effects of UV laser irradiation on Bacillus atrophaeus spore viability, structure, and proteins
spores are highly resistant to toxic chemicals and extreme environments. Because some species threaten public health, spore inactivation techniques have been intensively investigated. We exposed spores to a 266 nm Nd:YVO laser at a laser power of 1 W and various numbers of scans. As a result, the UV...
Gespeichert in:
| Veröffentlicht in: | Analyst (London) 2021-12, Vol.146 (24), p.7682-7692 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7692 |
|---|---|
| container_issue | 24 |
| container_start_page | 7682 |
| container_title | Analyst (London) |
| container_volume | 146 |
| creator | Nguyen, My-Chi Thi Nguyen, Huu-Quang Jang, Hanbyeol Noh, Sojung Lee, Seong-Yeon Jang, Kyoung-Soon Lee, Jaebeom Sohn, Youngku Yee, Kiju Jung, Heesoo Kim, Jeongkwon |
| description | spores are highly resistant to toxic chemicals and extreme environments. Because some
species threaten public health, spore inactivation techniques have been intensively investigated. We exposed
spores to a 266 nm Nd:YVO
laser at a laser power of 1 W and various numbers of scans. As a result, the UV laser reduced the viability of
spores. Although the outer coat of spores remained intact after UV laser irradiation of 720 scans, damage inside the spores was observed. Spore proteins were identified by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry during the course of UV laser irradiation. Photochemical and photothermal processes are believed to be involved in the UV laser sterilization of
spores. Our findings suggest that a UV laser is capable of sterilizing
spores. |
| doi_str_mv | 10.1039/d1an01717a |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2606827562</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2606827562</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-93c19e679b8f6202719c99d8ae754287b3de850cb006f7201214195120f674163</originalsourceid><addsrcrecordid>eNo9kF9LwzAUxYMobk5f_AAS8E1WzU3apHmc8y8MfdD5WtI2wYyurUkqzE9vdFO4cO_h_jgHDkKnQC6BMHlVg2oJCBBqD42B8TTJMprvozEhhCWUZ-kIHXm_ihJIRg7RiKU50JTJMVq_BO1sY79UsF2LtTG6Ch53Bi_fcKO8dtg6p2q7_ce5VpVtmsFjFVzXvysdT993TuNPq8poFTZT7IMbqjA4PcWqrXHvuqBt64_RgVGN1ye7PUHLu9vX-UOyeL5_nM8WSUVzGhLJKpCaC1nmhlNCBchKyjpXWmQpzUXJap1npCoJ4UZQAhRSkBlQYrhIgbMJOt_6xuCPQftQrLrBtTGyoJzwnIqM00hdbKnKdd47bYre2bVymwJI8dNscQOzp99mZxE-21kO5VrX_-hflewbGhBzJQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2606827562</pqid></control><display><type>article</type><title>Sterilization effects of UV laser irradiation on Bacillus atrophaeus spore viability, structure, and proteins</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals</source><source>Royal Society of Chemistry Journals Archive (1841-2007)</source><source>Alma/SFX Local Collection</source><creator>Nguyen, My-Chi Thi ; Nguyen, Huu-Quang ; Jang, Hanbyeol ; Noh, Sojung ; Lee, Seong-Yeon ; Jang, Kyoung-Soon ; Lee, Jaebeom ; Sohn, Youngku ; Yee, Kiju ; Jung, Heesoo ; Kim, Jeongkwon</creator><creatorcontrib>Nguyen, My-Chi Thi ; Nguyen, Huu-Quang ; Jang, Hanbyeol ; Noh, Sojung ; Lee, Seong-Yeon ; Jang, Kyoung-Soon ; Lee, Jaebeom ; Sohn, Youngku ; Yee, Kiju ; Jung, Heesoo ; Kim, Jeongkwon</creatorcontrib><description>spores are highly resistant to toxic chemicals and extreme environments. Because some
species threaten public health, spore inactivation techniques have been intensively investigated. We exposed
spores to a 266 nm Nd:YVO
laser at a laser power of 1 W and various numbers of scans. As a result, the UV laser reduced the viability of
spores. Although the outer coat of spores remained intact after UV laser irradiation of 720 scans, damage inside the spores was observed. Spore proteins were identified by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry during the course of UV laser irradiation. Photochemical and photothermal processes are believed to be involved in the UV laser sterilization of
spores. Our findings suggest that a UV laser is capable of sterilizing
spores.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/d1an01717a</identifier><identifier>PMID: 34812439</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Bacillus ; Cyclotron resonance ; Extreme environments ; Fourier transforms ; Ions ; Lasers ; Mass spectrometry ; Neodymium lasers ; Proteins ; Public health ; Radiation damage ; Spores ; Spores, Bacterial ; Sterilization ; Sterilization effects ; Ultraviolet lasers</subject><ispartof>Analyst (London), 2021-12, Vol.146 (24), p.7682-7692</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-93c19e679b8f6202719c99d8ae754287b3de850cb006f7201214195120f674163</citedby><cites>FETCH-LOGICAL-c282t-93c19e679b8f6202719c99d8ae754287b3de850cb006f7201214195120f674163</cites><orcidid>0000-0002-6655-0764 ; 0000-0002-8609-3038 ; 0000-0002-8414-7290 ; 0000-0002-0087-1151</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34812439$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, My-Chi Thi</creatorcontrib><creatorcontrib>Nguyen, Huu-Quang</creatorcontrib><creatorcontrib>Jang, Hanbyeol</creatorcontrib><creatorcontrib>Noh, Sojung</creatorcontrib><creatorcontrib>Lee, Seong-Yeon</creatorcontrib><creatorcontrib>Jang, Kyoung-Soon</creatorcontrib><creatorcontrib>Lee, Jaebeom</creatorcontrib><creatorcontrib>Sohn, Youngku</creatorcontrib><creatorcontrib>Yee, Kiju</creatorcontrib><creatorcontrib>Jung, Heesoo</creatorcontrib><creatorcontrib>Kim, Jeongkwon</creatorcontrib><title>Sterilization effects of UV laser irradiation on Bacillus atrophaeus spore viability, structure, and proteins</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>spores are highly resistant to toxic chemicals and extreme environments. Because some
species threaten public health, spore inactivation techniques have been intensively investigated. We exposed
spores to a 266 nm Nd:YVO
laser at a laser power of 1 W and various numbers of scans. As a result, the UV laser reduced the viability of
spores. Although the outer coat of spores remained intact after UV laser irradiation of 720 scans, damage inside the spores was observed. Spore proteins were identified by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry during the course of UV laser irradiation. Photochemical and photothermal processes are believed to be involved in the UV laser sterilization of
spores. Our findings suggest that a UV laser is capable of sterilizing
spores.</description><subject>Bacillus</subject><subject>Cyclotron resonance</subject><subject>Extreme environments</subject><subject>Fourier transforms</subject><subject>Ions</subject><subject>Lasers</subject><subject>Mass spectrometry</subject><subject>Neodymium lasers</subject><subject>Proteins</subject><subject>Public health</subject><subject>Radiation damage</subject><subject>Spores</subject><subject>Spores, Bacterial</subject><subject>Sterilization</subject><subject>Sterilization effects</subject><subject>Ultraviolet lasers</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF9LwzAUxYMobk5f_AAS8E1WzU3apHmc8y8MfdD5WtI2wYyurUkqzE9vdFO4cO_h_jgHDkKnQC6BMHlVg2oJCBBqD42B8TTJMprvozEhhCWUZ-kIHXm_ihJIRg7RiKU50JTJMVq_BO1sY79UsF2LtTG6Ch53Bi_fcKO8dtg6p2q7_ce5VpVtmsFjFVzXvysdT993TuNPq8poFTZT7IMbqjA4PcWqrXHvuqBt64_RgVGN1ye7PUHLu9vX-UOyeL5_nM8WSUVzGhLJKpCaC1nmhlNCBchKyjpXWmQpzUXJap1npCoJ4UZQAhRSkBlQYrhIgbMJOt_6xuCPQftQrLrBtTGyoJzwnIqM00hdbKnKdd47bYre2bVymwJI8dNscQOzp99mZxE-21kO5VrX_-hflewbGhBzJQ</recordid><startdate>20211206</startdate><enddate>20211206</enddate><creator>Nguyen, My-Chi Thi</creator><creator>Nguyen, Huu-Quang</creator><creator>Jang, Hanbyeol</creator><creator>Noh, Sojung</creator><creator>Lee, Seong-Yeon</creator><creator>Jang, Kyoung-Soon</creator><creator>Lee, Jaebeom</creator><creator>Sohn, Youngku</creator><creator>Yee, Kiju</creator><creator>Jung, Heesoo</creator><creator>Kim, Jeongkwon</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0002-6655-0764</orcidid><orcidid>https://orcid.org/0000-0002-8609-3038</orcidid><orcidid>https://orcid.org/0000-0002-8414-7290</orcidid><orcidid>https://orcid.org/0000-0002-0087-1151</orcidid></search><sort><creationdate>20211206</creationdate><title>Sterilization effects of UV laser irradiation on Bacillus atrophaeus spore viability, structure, and proteins</title><author>Nguyen, My-Chi Thi ; Nguyen, Huu-Quang ; Jang, Hanbyeol ; Noh, Sojung ; Lee, Seong-Yeon ; Jang, Kyoung-Soon ; Lee, Jaebeom ; Sohn, Youngku ; Yee, Kiju ; Jung, Heesoo ; Kim, Jeongkwon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-93c19e679b8f6202719c99d8ae754287b3de850cb006f7201214195120f674163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bacillus</topic><topic>Cyclotron resonance</topic><topic>Extreme environments</topic><topic>Fourier transforms</topic><topic>Ions</topic><topic>Lasers</topic><topic>Mass spectrometry</topic><topic>Neodymium lasers</topic><topic>Proteins</topic><topic>Public health</topic><topic>Radiation damage</topic><topic>Spores</topic><topic>Spores, Bacterial</topic><topic>Sterilization</topic><topic>Sterilization effects</topic><topic>Ultraviolet lasers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, My-Chi Thi</creatorcontrib><creatorcontrib>Nguyen, Huu-Quang</creatorcontrib><creatorcontrib>Jang, Hanbyeol</creatorcontrib><creatorcontrib>Noh, Sojung</creatorcontrib><creatorcontrib>Lee, Seong-Yeon</creatorcontrib><creatorcontrib>Jang, Kyoung-Soon</creatorcontrib><creatorcontrib>Lee, Jaebeom</creatorcontrib><creatorcontrib>Sohn, Youngku</creatorcontrib><creatorcontrib>Yee, Kiju</creatorcontrib><creatorcontrib>Jung, Heesoo</creatorcontrib><creatorcontrib>Kim, Jeongkwon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, My-Chi Thi</au><au>Nguyen, Huu-Quang</au><au>Jang, Hanbyeol</au><au>Noh, Sojung</au><au>Lee, Seong-Yeon</au><au>Jang, Kyoung-Soon</au><au>Lee, Jaebeom</au><au>Sohn, Youngku</au><au>Yee, Kiju</au><au>Jung, Heesoo</au><au>Kim, Jeongkwon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sterilization effects of UV laser irradiation on Bacillus atrophaeus spore viability, structure, and proteins</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2021-12-06</date><risdate>2021</risdate><volume>146</volume><issue>24</issue><spage>7682</spage><epage>7692</epage><pages>7682-7692</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>spores are highly resistant to toxic chemicals and extreme environments. Because some
species threaten public health, spore inactivation techniques have been intensively investigated. We exposed
spores to a 266 nm Nd:YVO
laser at a laser power of 1 W and various numbers of scans. As a result, the UV laser reduced the viability of
spores. Although the outer coat of spores remained intact after UV laser irradiation of 720 scans, damage inside the spores was observed. Spore proteins were identified by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry during the course of UV laser irradiation. Photochemical and photothermal processes are believed to be involved in the UV laser sterilization of
spores. Our findings suggest that a UV laser is capable of sterilizing
spores.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>34812439</pmid><doi>10.1039/d1an01717a</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6655-0764</orcidid><orcidid>https://orcid.org/0000-0002-8609-3038</orcidid><orcidid>https://orcid.org/0000-0002-8414-7290</orcidid><orcidid>https://orcid.org/0000-0002-0087-1151</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2654 |
| ispartof | Analyst (London), 2021-12, Vol.146 (24), p.7682-7692 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_proquest_journals_2606827562 |
| source | MEDLINE; Royal Society Of Chemistry Journals; Royal Society of Chemistry Journals Archive (1841-2007); Alma/SFX Local Collection |
| subjects | Bacillus Cyclotron resonance Extreme environments Fourier transforms Ions Lasers Mass spectrometry Neodymium lasers Proteins Public health Radiation damage Spores Spores, Bacterial Sterilization Sterilization effects Ultraviolet lasers |
| title | Sterilization effects of UV laser irradiation on Bacillus atrophaeus spore viability, structure, and proteins |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T18%3A57%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sterilization%20effects%20of%20UV%20laser%20irradiation%20on%20Bacillus%20atrophaeus%20spore%20viability,%20structure,%20and%20proteins&rft.jtitle=Analyst%20(London)&rft.au=Nguyen,%20My-Chi%20Thi&rft.date=2021-12-06&rft.volume=146&rft.issue=24&rft.spage=7682&rft.epage=7692&rft.pages=7682-7692&rft.issn=0003-2654&rft.eissn=1364-5528&rft_id=info:doi/10.1039/d1an01717a&rft_dat=%3Cproquest_cross%3E2606827562%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2606827562&rft_id=info:pmid/34812439&rfr_iscdi=true |