Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis?
This study aimed to evaluate the composition of the gastric microbiota in the gastric mucosa and gastric fluid of patients with chronic antral gastritis. Specifically, we sought to determine whether Helicobacter pylori (Hp) infection changes the bacterial community in the gastric mucosa or alters th...
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| Veröffentlicht in: | Journal of general and applied microbiology 2021, Vol.67(5), pp.179-185 |
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| creator | Jiang, Yang Meng, Fei Liu, Ying Zheng, Liyun Ye, Shufang Zhang, Jianmei |
| description | This study aimed to evaluate the composition of the gastric microbiota in the gastric mucosa and gastric fluid of patients with chronic antral gastritis. Specifically, we sought to determine whether Helicobacter pylori (Hp) infection changes the bacterial community in the gastric mucosa or alters the microbiota in the gastric fluid. The bacterial community at another site in the stomach was also investigated. DNA was extracted from 160 samples collected from 40 patients with chronic antral gastritis (20 Hp-positive and 20 Hp-negative cases). Three tissue samples of the gastric mucosa (gastric angle, body, and antral mucosa) and one tube of gastric fluid were collected from every patient. A 16S rRNA amplification library was created, and high-throughput sequencing was performed. A profile of the community composition was obtained using bioinformatics methods, including cluster, taxonomy, and diversity analyses. Analysis of the gastric bacterial community revealed that the community compositions of the gastric mucosa and gastric fluid of patients without Hp are similar to but show differences from those of Hp-positive patients. The microbiota in Hp-positive patients exhibited reduced microbial diversity, and the gastric fluid of these patients contained a small proportion of Hp. The richness of Leptotrichia in mucosal samples was greater than that in gastric fluid samples from Hp-negative patients with chronic antral gastritis. Hp changes the growth of other microbiota in the mucosa and affects the microbiota in the gastric fluid of patients with chronic antral gastritis. In addition to Hp, the presence of other bacteria might be related to the development of chronic antral gastritis. |
| doi_str_mv | 10.2323/jgam.2020.08.005 |
| format | Article |
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Specifically, we sought to determine whether Helicobacter pylori (Hp) infection changes the bacterial community in the gastric mucosa or alters the microbiota in the gastric fluid. The bacterial community at another site in the stomach was also investigated. DNA was extracted from 160 samples collected from 40 patients with chronic antral gastritis (20 Hp-positive and 20 Hp-negative cases). Three tissue samples of the gastric mucosa (gastric angle, body, and antral mucosa) and one tube of gastric fluid were collected from every patient. A 16S rRNA amplification library was created, and high-throughput sequencing was performed. A profile of the community composition was obtained using bioinformatics methods, including cluster, taxonomy, and diversity analyses. Analysis of the gastric bacterial community revealed that the community compositions of the gastric mucosa and gastric fluid of patients without Hp are similar to but show differences from those of Hp-positive patients. The microbiota in Hp-positive patients exhibited reduced microbial diversity, and the gastric fluid of these patients contained a small proportion of Hp. The richness of Leptotrichia in mucosal samples was greater than that in gastric fluid samples from Hp-negative patients with chronic antral gastritis. Hp changes the growth of other microbiota in the mucosa and affects the microbiota in the gastric fluid of patients with chronic antral gastritis. In addition to Hp, the presence of other bacteria might be related to the development of chronic antral gastritis.</description><identifier>ISSN: 0022-1260</identifier><identifier>EISSN: 1349-8037</identifier><identifier>DOI: 10.2323/jgam.2020.08.005</identifier><identifier>PMID: 34053980</identifier><language>eng</language><publisher>Japan: Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</publisher><subject>16SrRNA sequencing ; Adult ; Aged ; Bacteria ; Bacterial Physiological Phenomena ; bacterial structure ; Bioinformatics ; China ; chronic antral gastritis ; Community composition ; Composition ; Deoxyribonucleic acid ; DNA ; Female ; gastric fluid ; Gastric mucosa ; Gastric Mucosa - microbiology ; gastric mucosal ; Gastritis ; Gastrointestinal Microbiome - physiology ; Helicobacter Infections - microbiology ; Helicobacter pylori ; Helicobacter pylori - physiology ; Humans ; Infections ; Male ; Microbiota ; Microorganisms ; Middle Aged ; Next-generation sequencing ; rRNA 16S ; Taxonomy</subject><ispartof>The Journal of General and Applied Microbiology, 2021, Vol.67(5), pp.179-185</ispartof><rights>2021, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</rights><rights>Copyright Japan Science and Technology Agency 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-80f2f2b48281e6f33ed4c931766235e62088620f3eb94726ae7c963a3ecb7a183</citedby><cites>FETCH-LOGICAL-c522t-80f2f2b48281e6f33ed4c931766235e62088620f3eb94726ae7c963a3ecb7a183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34053980$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Yang</creatorcontrib><creatorcontrib>Meng, Fei</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Zheng, Liyun</creatorcontrib><creatorcontrib>Ye, Shufang</creatorcontrib><creatorcontrib>Zhang, Jianmei</creatorcontrib><title>Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis?</title><title>Journal of general and applied microbiology</title><addtitle>J. Gen. Appl. Microbiol.</addtitle><description>This study aimed to evaluate the composition of the gastric microbiota in the gastric mucosa and gastric fluid of patients with chronic antral gastritis. Specifically, we sought to determine whether Helicobacter pylori (Hp) infection changes the bacterial community in the gastric mucosa or alters the microbiota in the gastric fluid. The bacterial community at another site in the stomach was also investigated. DNA was extracted from 160 samples collected from 40 patients with chronic antral gastritis (20 Hp-positive and 20 Hp-negative cases). Three tissue samples of the gastric mucosa (gastric angle, body, and antral mucosa) and one tube of gastric fluid were collected from every patient. A 16S rRNA amplification library was created, and high-throughput sequencing was performed. A profile of the community composition was obtained using bioinformatics methods, including cluster, taxonomy, and diversity analyses. Analysis of the gastric bacterial community revealed that the community compositions of the gastric mucosa and gastric fluid of patients without Hp are similar to but show differences from those of Hp-positive patients. The microbiota in Hp-positive patients exhibited reduced microbial diversity, and the gastric fluid of these patients contained a small proportion of Hp. The richness of Leptotrichia in mucosal samples was greater than that in gastric fluid samples from Hp-negative patients with chronic antral gastritis. Hp changes the growth of other microbiota in the mucosa and affects the microbiota in the gastric fluid of patients with chronic antral gastritis. In addition to Hp, the presence of other bacteria might be related to the development of chronic antral gastritis.</description><subject>16SrRNA sequencing</subject><subject>Adult</subject><subject>Aged</subject><subject>Bacteria</subject><subject>Bacterial Physiological Phenomena</subject><subject>bacterial structure</subject><subject>Bioinformatics</subject><subject>China</subject><subject>chronic antral gastritis</subject><subject>Community composition</subject><subject>Composition</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Female</subject><subject>gastric fluid</subject><subject>Gastric mucosa</subject><subject>Gastric Mucosa - microbiology</subject><subject>gastric mucosal</subject><subject>Gastritis</subject><subject>Gastrointestinal Microbiome - physiology</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - physiology</subject><subject>Humans</subject><subject>Infections</subject><subject>Male</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Middle Aged</subject><subject>Next-generation sequencing</subject><subject>rRNA 16S</subject><subject>Taxonomy</subject><issn>0022-1260</issn><issn>1349-8037</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkUGP0zAQhS0EYrsLd07IEueWiZ04zgmhXZZFWokLnK2JO25dpXGxHaH9E_xmHNKtuIwtve89y_MYe1fBRkghPx52eNwIELABvQFoXrBVJeturUG2L9kKQIh1JRRcseuUDgBSCV2_ZleyhkZ2Glbsz12gxB9o8Db0aDNFfnoaQvTcj45s9mHk6OYbz3viKcfJ5ikSD44vvMeC_hN3WGRv-XGyISHHccvdMPntrJ8wexpz4r993nO7j2EsJI454nA2Zp8-vWGvHA6J3p7PG_bz_suP24f14_ev324_P65tI0Qu_3PCib7WQleknJS0rW0nq1YpIRtSArQuw0nqu7oVCqm1nZIoyfYtVlresA9L7imGXxOlbA5himN50pR1NSUJtCoULJSNIaVIzpyiP2J8MhWYuQAzF2DmAgxoUwoolvfn4Kk_0vZieN54Ae4X4JAy7ugCYMzeDrQkqtY08_gv-QLYPUZDo_wLwbicLQ</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Jiang, Yang</creator><creator>Meng, Fei</creator><creator>Liu, Ying</creator><creator>Zheng, Liyun</creator><creator>Ye, Shufang</creator><creator>Zhang, Jianmei</creator><general>Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20210101</creationdate><title>Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis?</title><author>Jiang, Yang ; Meng, Fei ; Liu, Ying ; Zheng, Liyun ; Ye, Shufang ; Zhang, Jianmei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-80f2f2b48281e6f33ed4c931766235e62088620f3eb94726ae7c963a3ecb7a183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>16SrRNA sequencing</topic><topic>Adult</topic><topic>Aged</topic><topic>Bacteria</topic><topic>Bacterial Physiological Phenomena</topic><topic>bacterial structure</topic><topic>Bioinformatics</topic><topic>China</topic><topic>chronic antral gastritis</topic><topic>Community composition</topic><topic>Composition</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Female</topic><topic>gastric fluid</topic><topic>Gastric mucosa</topic><topic>Gastric Mucosa - microbiology</topic><topic>gastric mucosal</topic><topic>Gastritis</topic><topic>Gastrointestinal Microbiome - physiology</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - physiology</topic><topic>Humans</topic><topic>Infections</topic><topic>Male</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Middle Aged</topic><topic>Next-generation sequencing</topic><topic>rRNA 16S</topic><topic>Taxonomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Yang</creatorcontrib><creatorcontrib>Meng, Fei</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Zheng, Liyun</creatorcontrib><creatorcontrib>Ye, Shufang</creatorcontrib><creatorcontrib>Zhang, Jianmei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of general and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Yang</au><au>Meng, Fei</au><au>Liu, Ying</au><au>Zheng, Liyun</au><au>Ye, Shufang</au><au>Zhang, Jianmei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis?</atitle><jtitle>Journal of general and applied microbiology</jtitle><addtitle>J. Gen. Appl. Microbiol.</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>67</volume><issue>5</issue><spage>179</spage><epage>185</epage><pages>179-185</pages><artnum>2020.08.005</artnum><issn>0022-1260</issn><eissn>1349-8037</eissn><abstract>This study aimed to evaluate the composition of the gastric microbiota in the gastric mucosa and gastric fluid of patients with chronic antral gastritis. Specifically, we sought to determine whether Helicobacter pylori (Hp) infection changes the bacterial community in the gastric mucosa or alters the microbiota in the gastric fluid. The bacterial community at another site in the stomach was also investigated. DNA was extracted from 160 samples collected from 40 patients with chronic antral gastritis (20 Hp-positive and 20 Hp-negative cases). Three tissue samples of the gastric mucosa (gastric angle, body, and antral mucosa) and one tube of gastric fluid were collected from every patient. A 16S rRNA amplification library was created, and high-throughput sequencing was performed. A profile of the community composition was obtained using bioinformatics methods, including cluster, taxonomy, and diversity analyses. Analysis of the gastric bacterial community revealed that the community compositions of the gastric mucosa and gastric fluid of patients without Hp are similar to but show differences from those of Hp-positive patients. The microbiota in Hp-positive patients exhibited reduced microbial diversity, and the gastric fluid of these patients contained a small proportion of Hp. The richness of Leptotrichia in mucosal samples was greater than that in gastric fluid samples from Hp-negative patients with chronic antral gastritis. Hp changes the growth of other microbiota in the mucosa and affects the microbiota in the gastric fluid of patients with chronic antral gastritis. In addition to Hp, the presence of other bacteria might be related to the development of chronic antral gastritis.</abstract><cop>Japan</cop><pub>Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</pub><pmid>34053980</pmid><doi>10.2323/jgam.2020.08.005</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 16SrRNA sequencing Adult Aged Bacteria Bacterial Physiological Phenomena bacterial structure Bioinformatics China chronic antral gastritis Community composition Composition Deoxyribonucleic acid DNA Female gastric fluid Gastric mucosa Gastric Mucosa - microbiology gastric mucosal Gastritis Gastrointestinal Microbiome - physiology Helicobacter Infections - microbiology Helicobacter pylori Helicobacter pylori - physiology Humans Infections Male Microbiota Microorganisms Middle Aged Next-generation sequencing rRNA 16S Taxonomy |
| title | Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis? |
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