An eco-friendly solvent-free reaction based on peptide probes: design an extraction-free method for analysis of acrylamide under microliter volume
Acrylamide is a group 2A carcinogen and potential endocrine disruptor that can enter the ecosystem by various routes and has recently become a dangerous pollutant. This widely used chemical can enter the human body via air inhalation, food or water consumption, or skin contact. In this study, we dev...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2021-12, Vol.413 (30), p.7531-7539 |
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| creator | Li, Yi-Shan Suen, Jau-Ling Tseng, Wei-Lung Lu, Chi-Yu |
| description | Acrylamide is a group 2A carcinogen and potential endocrine disruptor that can enter the ecosystem by various routes and has recently become a dangerous pollutant. This widely used chemical can enter the human body via air inhalation, food or water consumption, or skin contact. In this study, we developed a peptide probe for the detection of acrylamide by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) after its micro-tagging with a peptide. Direct detection of acrylamide by MALDI-TOF MS is not feasible due to its poor ionization in the MALDI interface, which hinders its analysis by the technique. After microwave irradiation for 2 min, the formed acrylamide-peptide derivative was detected easily by MALDI-TOF MS without the need for extraction procedures. The procedure does not involve organic solvents and a water-soluble peptide that allows detection of acrylamide in small sample volumes with a limit of detection (LOD) of 0.05 ng/μL. The relative standard deviation (RSD) and relative error (RE) of the measurements were |
| doi_str_mv | 10.1007/s00216-021-03717-6 |
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Graphical abstract</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-021-03717-6</identifier><identifier>PMID: 34635932</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acrylamide ; Acrylamide - analysis ; Acrylamide - urine ; Analysis ; Analytical Chemistry ; Animals ; Biochemistry ; Biological properties ; Biological samples ; Carcinogens ; Cell Line ; Characterization and Evaluation of Materials ; Chemical reactions ; Chemistry ; Chemistry and Materials Science ; Epithelial cells ; Epithelial Cells - chemistry ; Epithelium ; Error analysis ; Extraction (Chemistry) ; Extraction procedures ; Food consumption ; Food Science ; Humans ; Identification and classification ; Inhalation ; Ionization ; Ions ; Irradiation ; Laboratory Medicine ; Limit of Detection ; Lung - chemistry ; Lung - cytology ; Marking and tracking techniques ; Mass spectrometry ; Mass spectroscopy ; Methods ; Mice ; Miniaturization ; Molecular Probes - chemistry ; Monitoring/Environmental Analysis ; Organic solvents ; Peptides ; Peptides - chemistry ; Pollutants ; Polyacrylamide ; Properties ; Reagents ; Reproducibility of Results ; Research Paper ; Respiration ; Solvents ; Solvents - chemistry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Water consumption</subject><ispartof>Analytical and bioanalytical chemistry, 2021-12, Vol.413 (30), p.7531-7539</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>2021. Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>COPYRIGHT 2021 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c365t-eece088052711009845829efc8b8c4a7847b63b5e5940fa4978c96042dfb372b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-021-03717-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-021-03717-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34635932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yi-Shan</creatorcontrib><creatorcontrib>Suen, Jau-Ling</creatorcontrib><creatorcontrib>Tseng, Wei-Lung</creatorcontrib><creatorcontrib>Lu, Chi-Yu</creatorcontrib><title>An eco-friendly solvent-free reaction based on peptide probes: design an extraction-free method for analysis of acrylamide under microliter volume</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Acrylamide is a group 2A carcinogen and potential endocrine disruptor that can enter the ecosystem by various routes and has recently become a dangerous pollutant. This widely used chemical can enter the human body via air inhalation, food or water consumption, or skin contact. In this study, we developed a peptide probe for the detection of acrylamide by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) after its micro-tagging with a peptide. Direct detection of acrylamide by MALDI-TOF MS is not feasible due to its poor ionization in the MALDI interface, which hinders its analysis by the technique. After microwave irradiation for 2 min, the formed acrylamide-peptide derivative was detected easily by MALDI-TOF MS without the need for extraction procedures. The procedure does not involve organic solvents and a water-soluble peptide that allows detection of acrylamide in small sample volumes with a limit of detection (LOD) of 0.05 ng/μL. The relative standard deviation (RSD) and relative error (RE) of the measurements were < 6.7% for intra- and inter-day assays. Gel-washing solutions from a polyacrylamide gel experiment were used as a model to study the efficiency of the developed method. Finally, we used the proposed method for the detection of free acrylamide in small volumes of lung epithelial cells (a model to test the air inhalation of acrylamide under a tiny volume of sample) and human urine. The developed method will enable rapid acrylamide detection in environmental and biological samples via a green approach based on microwave-assisted derivatization in water alongside the use of a less toxic derivatization reagent, reusable target plate, and miniaturization protocols.
Graphical abstract</description><subject>Acrylamide</subject><subject>Acrylamide - analysis</subject><subject>Acrylamide - urine</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological properties</subject><subject>Biological samples</subject><subject>Carcinogens</subject><subject>Cell Line</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical reactions</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - chemistry</subject><subject>Epithelium</subject><subject>Error analysis</subject><subject>Extraction (Chemistry)</subject><subject>Extraction procedures</subject><subject>Food consumption</subject><subject>Food Science</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Inhalation</subject><subject>Ionization</subject><subject>Ions</subject><subject>Irradiation</subject><subject>Laboratory Medicine</subject><subject>Limit of Detection</subject><subject>Lung - chemistry</subject><subject>Lung - cytology</subject><subject>Marking and tracking techniques</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Methods</subject><subject>Mice</subject><subject>Miniaturization</subject><subject>Molecular Probes - chemistry</subject><subject>Monitoring/Environmental Analysis</subject><subject>Organic solvents</subject><subject>Peptides</subject><subject>Peptides - chemistry</subject><subject>Pollutants</subject><subject>Polyacrylamide</subject><subject>Properties</subject><subject>Reagents</subject><subject>Reproducibility of Results</subject><subject>Research Paper</subject><subject>Respiration</subject><subject>Solvents</subject><subject>Solvents - chemistry</subject><subject>Spectrometry, 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eco-friendly solvent-free reaction based on peptide probes: design an extraction-free method for analysis of acrylamide under microliter volume</title><author>Li, Yi-Shan ; Suen, Jau-Ling ; Tseng, Wei-Lung ; Lu, Chi-Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-eece088052711009845829efc8b8c4a7847b63b5e5940fa4978c96042dfb372b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acrylamide</topic><topic>Acrylamide - analysis</topic><topic>Acrylamide - urine</topic><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological properties</topic><topic>Biological samples</topic><topic>Carcinogens</topic><topic>Cell Line</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical reactions</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - chemistry</topic><topic>Epithelium</topic><topic>Error analysis</topic><topic>Extraction (Chemistry)</topic><topic>Extraction procedures</topic><topic>Food consumption</topic><topic>Food Science</topic><topic>Humans</topic><topic>Identification and classification</topic><topic>Inhalation</topic><topic>Ionization</topic><topic>Ions</topic><topic>Irradiation</topic><topic>Laboratory Medicine</topic><topic>Limit of Detection</topic><topic>Lung - chemistry</topic><topic>Lung - cytology</topic><topic>Marking and tracking techniques</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Methods</topic><topic>Mice</topic><topic>Miniaturization</topic><topic>Molecular Probes - chemistry</topic><topic>Monitoring/Environmental Analysis</topic><topic>Organic solvents</topic><topic>Peptides</topic><topic>Peptides - 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Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>413</volume><issue>30</issue><spage>7531</spage><epage>7539</epage><pages>7531-7539</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Acrylamide is a group 2A carcinogen and potential endocrine disruptor that can enter the ecosystem by various routes and has recently become a dangerous pollutant. This widely used chemical can enter the human body via air inhalation, food or water consumption, or skin contact. In this study, we developed a peptide probe for the detection of acrylamide by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) after its micro-tagging with a peptide. Direct detection of acrylamide by MALDI-TOF MS is not feasible due to its poor ionization in the MALDI interface, which hinders its analysis by the technique. After microwave irradiation for 2 min, the formed acrylamide-peptide derivative was detected easily by MALDI-TOF MS without the need for extraction procedures. The procedure does not involve organic solvents and a water-soluble peptide that allows detection of acrylamide in small sample volumes with a limit of detection (LOD) of 0.05 ng/μL. The relative standard deviation (RSD) and relative error (RE) of the measurements were < 6.7% for intra- and inter-day assays. Gel-washing solutions from a polyacrylamide gel experiment were used as a model to study the efficiency of the developed method. Finally, we used the proposed method for the detection of free acrylamide in small volumes of lung epithelial cells (a model to test the air inhalation of acrylamide under a tiny volume of sample) and human urine. The developed method will enable rapid acrylamide detection in environmental and biological samples via a green approach based on microwave-assisted derivatization in water alongside the use of a less toxic derivatization reagent, reusable target plate, and miniaturization protocols.
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| subjects | Acrylamide Acrylamide - analysis Acrylamide - urine Analysis Analytical Chemistry Animals Biochemistry Biological properties Biological samples Carcinogens Cell Line Characterization and Evaluation of Materials Chemical reactions Chemistry Chemistry and Materials Science Epithelial cells Epithelial Cells - chemistry Epithelium Error analysis Extraction (Chemistry) Extraction procedures Food consumption Food Science Humans Identification and classification Inhalation Ionization Ions Irradiation Laboratory Medicine Limit of Detection Lung - chemistry Lung - cytology Marking and tracking techniques Mass spectrometry Mass spectroscopy Methods Mice Miniaturization Molecular Probes - chemistry Monitoring/Environmental Analysis Organic solvents Peptides Peptides - chemistry Pollutants Polyacrylamide Properties Reagents Reproducibility of Results Research Paper Respiration Solvents Solvents - chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Water consumption |
| title | An eco-friendly solvent-free reaction based on peptide probes: design an extraction-free method for analysis of acrylamide under microliter volume |
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