Arabidopsis MADS‐box factor AGL16 is a negative regulator of plant response to salt stress by downregulating salt‐responsive genes
Summary Sessile plants constantly experience environmental stresses in nature. They must have evolved effective mechanisms to balance growth with stress response. Here we report the MADS‐box transcription factor AGL16 acting as a negative regulator in stress response in Arabidopsis. Loss‐of‐AGL16 co...
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Veröffentlicht in: | The New phytologist 2021-12, Vol.232 (6), p.2418-2439 |
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creator | Zhao, Ping‐Xia Zhang, Jing Chen, Si‐Yan Wu, Jie Xia, Jing‐Qiu Sun, Liang‐Qi Ma, Shi‐Song Xiang, Cheng‐Bin |
description | Summary
Sessile plants constantly experience environmental stresses in nature. They must have evolved effective mechanisms to balance growth with stress response. Here we report the MADS‐box transcription factor AGL16 acting as a negative regulator in stress response in Arabidopsis.
Loss‐of‐AGL16 confers resistance to salt stress in seed germination, root elongation and soil‐grown plants, while elevated AGL16 expression confers the opposite phenotypes compared with wild‐type. However, the sensitivity to abscisic acid (ABA) in seed germination is inversely correlated with AGL16 expression levels.
Transcriptomic comparison revealed that the improved salt resistance of agl16 mutants was largely attributed to enhanced expression of stress‐responsive transcriptional factors and the genes involved in ABA signalling and ion homeostasis. We further demonstrated that AGL16 directly binds to the CArG motifs in the promoter of HKT1;1, HsfA6a and MYB102 and represses their expression. Genetic analyses with double mutants also support that HsfA6a and MYB102 are target genes of AGL16.
Taken together, our results show that AGL16 acts as a negative regulator transcriptionally suppressing key components in the stress response and may play a role in balancing stress response with growth. |
doi_str_mv | 10.1111/nph.17760 |
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Sessile plants constantly experience environmental stresses in nature. They must have evolved effective mechanisms to balance growth with stress response. Here we report the MADS‐box transcription factor AGL16 acting as a negative regulator in stress response in Arabidopsis.
Loss‐of‐AGL16 confers resistance to salt stress in seed germination, root elongation and soil‐grown plants, while elevated AGL16 expression confers the opposite phenotypes compared with wild‐type. However, the sensitivity to abscisic acid (ABA) in seed germination is inversely correlated with AGL16 expression levels.
Transcriptomic comparison revealed that the improved salt resistance of agl16 mutants was largely attributed to enhanced expression of stress‐responsive transcriptional factors and the genes involved in ABA signalling and ion homeostasis. We further demonstrated that AGL16 directly binds to the CArG motifs in the promoter of HKT1;1, HsfA6a and MYB102 and represses their expression. Genetic analyses with double mutants also support that HsfA6a and MYB102 are target genes of AGL16.
Taken together, our results show that AGL16 acts as a negative regulator transcriptionally suppressing key components in the stress response and may play a role in balancing stress response with growth.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.17760</identifier><identifier>PMID: 34605021</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>ABA signalling ; Abiotic stress ; Abscisic Acid ; AGL16 ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Down-regulation ; Elongation ; Environmental stress ; Gene Expression Regulation, Plant ; Genes ; Genetic analysis ; Germination ; Germination - genetics ; Homeostasis ; MADS‐box ; Mutants ; Phenotypes ; Plants, Genetically Modified - metabolism ; primary root elongation ; Salt Stress ; Salts ; Seed germination ; Seedlings - metabolism ; Stress response ; Stress, Physiological - genetics ; Transcription ; Transcription factors ; Transcriptomics</subject><ispartof>The New phytologist, 2021-12, Vol.232 (6), p.2418-2439</ispartof><rights>2021 The Authors. © 2021 New Phytologist Foundation</rights><rights>2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.</rights><rights>Copyright © 2021 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3880-4f99befc4f2a5d10f68ee578c77b4e8e8dd02534c8e43a72679f5f9f05dd31633</citedby><cites>FETCH-LOGICAL-c3880-4f99befc4f2a5d10f68ee578c77b4e8e8dd02534c8e43a72679f5f9f05dd31633</cites><orcidid>0000-0002-7152-1458</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fnph.17760$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fnph.17760$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34605021$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Ping‐Xia</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Chen, Si‐Yan</creatorcontrib><creatorcontrib>Wu, Jie</creatorcontrib><creatorcontrib>Xia, Jing‐Qiu</creatorcontrib><creatorcontrib>Sun, Liang‐Qi</creatorcontrib><creatorcontrib>Ma, Shi‐Song</creatorcontrib><creatorcontrib>Xiang, Cheng‐Bin</creatorcontrib><title>Arabidopsis MADS‐box factor AGL16 is a negative regulator of plant response to salt stress by downregulating salt‐responsive genes</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Summary
Sessile plants constantly experience environmental stresses in nature. They must have evolved effective mechanisms to balance growth with stress response. Here we report the MADS‐box transcription factor AGL16 acting as a negative regulator in stress response in Arabidopsis.
Loss‐of‐AGL16 confers resistance to salt stress in seed germination, root elongation and soil‐grown plants, while elevated AGL16 expression confers the opposite phenotypes compared with wild‐type. However, the sensitivity to abscisic acid (ABA) in seed germination is inversely correlated with AGL16 expression levels.
Transcriptomic comparison revealed that the improved salt resistance of agl16 mutants was largely attributed to enhanced expression of stress‐responsive transcriptional factors and the genes involved in ABA signalling and ion homeostasis. We further demonstrated that AGL16 directly binds to the CArG motifs in the promoter of HKT1;1, HsfA6a and MYB102 and represses their expression. Genetic analyses with double mutants also support that HsfA6a and MYB102 are target genes of AGL16.
Taken together, our results show that AGL16 acts as a negative regulator transcriptionally suppressing key components in the stress response and may play a role in balancing stress response with growth.</description><subject>ABA signalling</subject><subject>Abiotic stress</subject><subject>Abscisic Acid</subject><subject>AGL16</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Down-regulation</subject><subject>Elongation</subject><subject>Environmental stress</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genetic analysis</subject><subject>Germination</subject><subject>Germination - genetics</subject><subject>Homeostasis</subject><subject>MADS‐box</subject><subject>Mutants</subject><subject>Phenotypes</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>primary root elongation</subject><subject>Salt Stress</subject><subject>Salts</subject><subject>Seed germination</subject><subject>Seedlings - metabolism</subject><subject>Stress response</subject><subject>Stress, Physiological - genetics</subject><subject>Transcription</subject><subject>Transcription factors</subject><subject>Transcriptomics</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLtOwzAUhi0EoqUw8ALIEhNDWjtOHGesuLRI5SIBElvkJMclVRoHO6F0Y2LmGXkS3AtseLF0_u98R_oROqakT90bVPVLn0YRJzuoSwMee4KyaBd1CfGFxwP-3EEH1s4IIXHI_X3UYQEnIfFpF30OjUyLXNe2sPhmePHw_fGV6nesZNZog4ejCeXYRRJXMJVN8QbYwLQt5SrVCtelrBo3srWuLOBGYyvLBtvGjSxOlzjXi2q7UVTTdepObBdWuilUYA_RnpKlhaPt30NPV5eP52Nvcje6Ph9OvIwJQbxAxXEKKguUL8OcEsUFQBiJLIrSAASIPCd-yIJMQMBk5PMoVqGKFQnznFHOWA-dbry10a8t2CaZ6dZU7mTih7FgMRE-cdTZhsqMttaASmpTzKVZJpQkq8YT13iybtyxJ1tjm84h_yN_K3bAYAMsihKW_5uS2_vxRvkDW86OHg</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Zhao, Ping‐Xia</creator><creator>Zhang, Jing</creator><creator>Chen, Si‐Yan</creator><creator>Wu, Jie</creator><creator>Xia, Jing‐Qiu</creator><creator>Sun, Liang‐Qi</creator><creator>Ma, Shi‐Song</creator><creator>Xiang, Cheng‐Bin</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-7152-1458</orcidid></search><sort><creationdate>202112</creationdate><title>Arabidopsis MADS‐box factor AGL16 is a negative regulator of plant response to salt stress by downregulating salt‐responsive genes</title><author>Zhao, Ping‐Xia ; Zhang, Jing ; Chen, Si‐Yan ; Wu, Jie ; Xia, Jing‐Qiu ; Sun, Liang‐Qi ; Ma, Shi‐Song ; Xiang, Cheng‐Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3880-4f99befc4f2a5d10f68ee578c77b4e8e8dd02534c8e43a72679f5f9f05dd31633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>ABA signalling</topic><topic>Abiotic stress</topic><topic>Abscisic Acid</topic><topic>AGL16</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Down-regulation</topic><topic>Elongation</topic><topic>Environmental stress</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genetic analysis</topic><topic>Germination</topic><topic>Germination - genetics</topic><topic>Homeostasis</topic><topic>MADS‐box</topic><topic>Mutants</topic><topic>Phenotypes</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>primary root elongation</topic><topic>Salt Stress</topic><topic>Salts</topic><topic>Seed germination</topic><topic>Seedlings - metabolism</topic><topic>Stress response</topic><topic>Stress, Physiological - genetics</topic><topic>Transcription</topic><topic>Transcription factors</topic><topic>Transcriptomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Ping‐Xia</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Chen, Si‐Yan</creatorcontrib><creatorcontrib>Wu, Jie</creatorcontrib><creatorcontrib>Xia, Jing‐Qiu</creatorcontrib><creatorcontrib>Sun, Liang‐Qi</creatorcontrib><creatorcontrib>Ma, Shi‐Song</creatorcontrib><creatorcontrib>Xiang, Cheng‐Bin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Ping‐Xia</au><au>Zhang, Jing</au><au>Chen, Si‐Yan</au><au>Wu, Jie</au><au>Xia, Jing‐Qiu</au><au>Sun, Liang‐Qi</au><au>Ma, Shi‐Song</au><au>Xiang, Cheng‐Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arabidopsis MADS‐box factor AGL16 is a negative regulator of plant response to salt stress by downregulating salt‐responsive genes</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2021-12</date><risdate>2021</risdate><volume>232</volume><issue>6</issue><spage>2418</spage><epage>2439</epage><pages>2418-2439</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Summary
Sessile plants constantly experience environmental stresses in nature. They must have evolved effective mechanisms to balance growth with stress response. Here we report the MADS‐box transcription factor AGL16 acting as a negative regulator in stress response in Arabidopsis.
Loss‐of‐AGL16 confers resistance to salt stress in seed germination, root elongation and soil‐grown plants, while elevated AGL16 expression confers the opposite phenotypes compared with wild‐type. However, the sensitivity to abscisic acid (ABA) in seed germination is inversely correlated with AGL16 expression levels.
Transcriptomic comparison revealed that the improved salt resistance of agl16 mutants was largely attributed to enhanced expression of stress‐responsive transcriptional factors and the genes involved in ABA signalling and ion homeostasis. We further demonstrated that AGL16 directly binds to the CArG motifs in the promoter of HKT1;1, HsfA6a and MYB102 and represses their expression. Genetic analyses with double mutants also support that HsfA6a and MYB102 are target genes of AGL16.
Taken together, our results show that AGL16 acts as a negative regulator transcriptionally suppressing key components in the stress response and may play a role in balancing stress response with growth.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34605021</pmid><doi>10.1111/nph.17760</doi><tpages>22</tpages><orcidid>https://orcid.org/0000-0002-7152-1458</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | ABA signalling Abiotic stress Abscisic Acid AGL16 Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Down-regulation Elongation Environmental stress Gene Expression Regulation, Plant Genes Genetic analysis Germination Germination - genetics Homeostasis MADS‐box Mutants Phenotypes Plants, Genetically Modified - metabolism primary root elongation Salt Stress Salts Seed germination Seedlings - metabolism Stress response Stress, Physiological - genetics Transcription Transcription factors Transcriptomics |
title | Arabidopsis MADS‐box factor AGL16 is a negative regulator of plant response to salt stress by downregulating salt‐responsive genes |
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