Arabidopsis MADS‐box factor AGL16 is a negative regulator of plant response to salt stress by downregulating salt‐responsive genes

Summary Sessile plants constantly experience environmental stresses in nature. They must have evolved effective mechanisms to balance growth with stress response. Here we report the MADS‐box transcription factor AGL16 acting as a negative regulator in stress response in Arabidopsis. Loss‐of‐AGL16 co...

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Veröffentlicht in:The New phytologist 2021-12, Vol.232 (6), p.2418-2439
Hauptverfasser: Zhao, Ping‐Xia, Zhang, Jing, Chen, Si‐Yan, Wu, Jie, Xia, Jing‐Qiu, Sun, Liang‐Qi, Ma, Shi‐Song, Xiang, Cheng‐Bin
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container_end_page 2439
container_issue 6
container_start_page 2418
container_title The New phytologist
container_volume 232
creator Zhao, Ping‐Xia
Zhang, Jing
Chen, Si‐Yan
Wu, Jie
Xia, Jing‐Qiu
Sun, Liang‐Qi
Ma, Shi‐Song
Xiang, Cheng‐Bin
description Summary Sessile plants constantly experience environmental stresses in nature. They must have evolved effective mechanisms to balance growth with stress response. Here we report the MADS‐box transcription factor AGL16 acting as a negative regulator in stress response in Arabidopsis. Loss‐of‐AGL16 confers resistance to salt stress in seed germination, root elongation and soil‐grown plants, while elevated AGL16 expression confers the opposite phenotypes compared with wild‐type. However, the sensitivity to abscisic acid (ABA) in seed germination is inversely correlated with AGL16 expression levels. Transcriptomic comparison revealed that the improved salt resistance of agl16 mutants was largely attributed to enhanced expression of stress‐responsive transcriptional factors and the genes involved in ABA signalling and ion homeostasis. We further demonstrated that AGL16 directly binds to the CArG motifs in the promoter of HKT1;1, HsfA6a and MYB102 and represses their expression. Genetic analyses with double mutants also support that HsfA6a and MYB102 are target genes of AGL16. Taken together, our results show that AGL16 acts as a negative regulator transcriptionally suppressing key components in the stress response and may play a role in balancing stress response with growth.
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They must have evolved effective mechanisms to balance growth with stress response. Here we report the MADS‐box transcription factor AGL16 acting as a negative regulator in stress response in Arabidopsis. Loss‐of‐AGL16 confers resistance to salt stress in seed germination, root elongation and soil‐grown plants, while elevated AGL16 expression confers the opposite phenotypes compared with wild‐type. However, the sensitivity to abscisic acid (ABA) in seed germination is inversely correlated with AGL16 expression levels. Transcriptomic comparison revealed that the improved salt resistance of agl16 mutants was largely attributed to enhanced expression of stress‐responsive transcriptional factors and the genes involved in ABA signalling and ion homeostasis. We further demonstrated that AGL16 directly binds to the CArG motifs in the promoter of HKT1;1, HsfA6a and MYB102 and represses their expression. Genetic analyses with double mutants also support that HsfA6a and MYB102 are target genes of AGL16. 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Genetic analyses with double mutants also support that HsfA6a and MYB102 are target genes of AGL16. 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subjects ABA signalling
Abiotic stress
Abscisic Acid
AGL16
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Down-regulation
Elongation
Environmental stress
Gene Expression Regulation, Plant
Genes
Genetic analysis
Germination
Germination - genetics
Homeostasis
MADS‐box
Mutants
Phenotypes
Plants, Genetically Modified - metabolism
primary root elongation
Salt Stress
Salts
Seed germination
Seedlings - metabolism
Stress response
Stress, Physiological - genetics
Transcription
Transcription factors
Transcriptomics
title Arabidopsis MADS‐box factor AGL16 is a negative regulator of plant response to salt stress by downregulating salt‐responsive genes
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