Tumor Tissue Oxidative Stress Changes and Na, K-ATPase Evaluation in Head and Neck Squamous Cell Carcinoma
Changes in metabolism are mechanisms that are largely implicated in the development, progression, and metastasis of head and neck squamous cell carcinoma (HNSCC) and also in resistance to different anticancer therapies. Identification of biomarkers for differentiation between cancerous and normal ep...
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| Veröffentlicht in: | The Journal of membrane biology 2021-12, Vol.254 (5-6), p.475-486 |
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| creator | Toledo, Marina Marques De Souza Gonçalves, Bruno Colodette, Natalie Mounteer Chaves, Aline Lauda Freitas Muniz, Luciana Vieira De A. Ribeiro, Rosy Iara Maciel Dos Santos, Hélio Batista Cortes, Vanessa F. Soares, Joao Marcos Arantes De Lima Santos, Hérica Barbosa, Leandro A. |
| description | Changes in metabolism are mechanisms that are largely implicated in the development, progression, and metastasis of head and neck squamous cell carcinoma (HNSCC) and also in resistance to different anticancer therapies. Identification of biomarkers for differentiation between cancerous and normal epithelium, treatment design and prognosis remain a vital issue in the field of head and neck cancer. The present study analyzed the main biochemical changes that occur in HNSCC tumors by through mechanisms involving oxidative stress. The release of substances reactive to thiobarbituric acid was significantly lower in HNSCC tumor tissue as compared to healthy tissue. The assays related to the lipid profile assays showed changes in membrane biophysics of tumor cells due to an increase in total phospholipids and total cholesterol, as well as an increased activity and expression of the α1 subunit of Na, K-ATPase, which is fundamental in the process of carcinogenesis. The modulation of the antioxidant system was also affected, with a decrease in the catalytic activity of the enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), as well as a reduction of glutathione (GSH) content and an increase in H
2
O
2
content. A reduction in catalase (CAT) activity was observed. The data presented here are in accordance with important findings described by us in a previous study, involving the same individuals, but with a focus on the damage generated in red blood cells, resulting from tumor installation. Therefore, it was possible to conclude that the biochemical alterations found in HNSCC cells are fundamental for transformation and maintenance of the tumor cell and once it is installed, it is also capable of generating injuries in the patients' red blood cells. Our data demonstrate that this could be a promising biomarker for HNSCC.
Graphic Abstract |
| doi_str_mv | 10.1007/s00232-021-00185-y |
| format | Article |
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2
O
2
content. A reduction in catalase (CAT) activity was observed. The data presented here are in accordance with important findings described by us in a previous study, involving the same individuals, but with a focus on the damage generated in red blood cells, resulting from tumor installation. Therefore, it was possible to conclude that the biochemical alterations found in HNSCC cells are fundamental for transformation and maintenance of the tumor cell and once it is installed, it is also capable of generating injuries in the patients' red blood cells. Our data demonstrate that this could be a promising biomarker for HNSCC.
Graphic Abstract</description><identifier>ISSN: 0022-2631</identifier><identifier>EISSN: 1432-1424</identifier><identifier>DOI: 10.1007/s00232-021-00185-y</identifier><identifier>PMID: 34104985</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenosine Triphosphatases ; Antioxidants ; Biochemistry ; Biomarkers ; Biomedical and Life Sciences ; Biophysics ; Carcinogenesis ; Carcinogens ; Catalase ; Catalytic activity ; Cholesterol ; Epithelium ; Erythrocytes ; Glutathione ; Glutathione peroxidase ; Head & neck cancer ; Head and neck carcinoma ; Head and Neck Neoplasms ; Human Physiology ; Humans ; Hydrogen Peroxide ; Life Sciences ; Lipids ; Metastases ; Na+/K+-exchanging ATPase ; Na/K-ATPase Ion Transport and Receptor Mediated Signaling Pathways ; Oxidative Stress ; Peroxidase ; Phospholipids ; Reduction ; Squamous cell carcinoma ; Squamous Cell Carcinoma of Head and Neck ; Superoxide dismutase ; Thiobarbituric acid ; Tumor cells ; Tumors</subject><ispartof>The Journal of membrane biology, 2021-12, Vol.254 (5-6), p.475-486</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-42dcd7b361f434d2292828c90ccbe391df01e0834c254d7370ab57718da0eccc3</citedby><cites>FETCH-LOGICAL-c375t-42dcd7b361f434d2292828c90ccbe391df01e0834c254d7370ab57718da0eccc3</cites><orcidid>0000-0002-4631-0130</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00232-021-00185-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00232-021-00185-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34104985$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toledo, Marina Marques</creatorcontrib><creatorcontrib>De Souza Gonçalves, Bruno</creatorcontrib><creatorcontrib>Colodette, Natalie Mounteer</creatorcontrib><creatorcontrib>Chaves, Aline Lauda Freitas</creatorcontrib><creatorcontrib>Muniz, Luciana Vieira</creatorcontrib><creatorcontrib>De A. Ribeiro, Rosy Iara Maciel</creatorcontrib><creatorcontrib>Dos Santos, Hélio Batista</creatorcontrib><creatorcontrib>Cortes, Vanessa F.</creatorcontrib><creatorcontrib>Soares, Joao Marcos Arantes</creatorcontrib><creatorcontrib>De Lima Santos, Hérica</creatorcontrib><creatorcontrib>Barbosa, Leandro A.</creatorcontrib><title>Tumor Tissue Oxidative Stress Changes and Na, K-ATPase Evaluation in Head and Neck Squamous Cell Carcinoma</title><title>The Journal of membrane biology</title><addtitle>J Membrane Biol</addtitle><addtitle>J Membr Biol</addtitle><description>Changes in metabolism are mechanisms that are largely implicated in the development, progression, and metastasis of head and neck squamous cell carcinoma (HNSCC) and also in resistance to different anticancer therapies. Identification of biomarkers for differentiation between cancerous and normal epithelium, treatment design and prognosis remain a vital issue in the field of head and neck cancer. The present study analyzed the main biochemical changes that occur in HNSCC tumors by through mechanisms involving oxidative stress. The release of substances reactive to thiobarbituric acid was significantly lower in HNSCC tumor tissue as compared to healthy tissue. The assays related to the lipid profile assays showed changes in membrane biophysics of tumor cells due to an increase in total phospholipids and total cholesterol, as well as an increased activity and expression of the α1 subunit of Na, K-ATPase, which is fundamental in the process of carcinogenesis. The modulation of the antioxidant system was also affected, with a decrease in the catalytic activity of the enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), as well as a reduction of glutathione (GSH) content and an increase in H
2
O
2
content. A reduction in catalase (CAT) activity was observed. The data presented here are in accordance with important findings described by us in a previous study, involving the same individuals, but with a focus on the damage generated in red blood cells, resulting from tumor installation. Therefore, it was possible to conclude that the biochemical alterations found in HNSCC cells are fundamental for transformation and maintenance of the tumor cell and once it is installed, it is also capable of generating injuries in the patients' red blood cells. Our data demonstrate that this could be a promising biomarker for HNSCC.
Graphic Abstract</description><subject>Adenosine Triphosphatases</subject><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Biophysics</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Catalase</subject><subject>Catalytic activity</subject><subject>Cholesterol</subject><subject>Epithelium</subject><subject>Erythrocytes</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Head & neck cancer</subject><subject>Head and neck carcinoma</subject><subject>Head and Neck Neoplasms</subject><subject>Human Physiology</subject><subject>Humans</subject><subject>Hydrogen Peroxide</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Metastases</subject><subject>Na+/K+-exchanging ATPase</subject><subject>Na/K-ATPase Ion Transport and Receptor Mediated Signaling Pathways</subject><subject>Oxidative Stress</subject><subject>Peroxidase</subject><subject>Phospholipids</subject><subject>Reduction</subject><subject>Squamous cell carcinoma</subject><subject>Squamous Cell Carcinoma of Head and Neck</subject><subject>Superoxide dismutase</subject><subject>Thiobarbituric acid</subject><subject>Tumor cells</subject><subject>Tumors</subject><issn>0022-2631</issn><issn>1432-1424</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kMtOwzAQRS0EouXxAyyQJbYE_EqcLKuoUERFkRrWlmM7kJJHaycV_XsMKbBjNdLMuXekA8AFRjcYIX7rECKUBIjgACEch8HuAIwx8yvMCDsEY38nAYkoHoET51Ye4jxix2BEGUYsicMxWGV93VqYlc71Bi4-Si27cmvgsrPGOZi-yebVOCgbDZ_kNXwMJtmzdAZOt7LqPdo2sGzgzEg9MEa9w-Wml3Xb-7SpKphKq8qmreUZOCpk5cz5fp6Cl7tpls6C-eL-IZ3MA0V52AWMaKV5TiNcMMo0IQmJSawSpFRuaIJ1gbBBMWWKhExzypHMQ85xrCUySil6Cq6G3rVtN71xnVi1vW38S0HChCc04ox5igyUsq1z1hRibcta2p3ASHzpFYNe4fWKb71i50OX--o-r43-jfz49AAdAOdP3pz9-_1P7Sd12YSX</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Toledo, Marina Marques</creator><creator>De Souza Gonçalves, Bruno</creator><creator>Colodette, Natalie Mounteer</creator><creator>Chaves, Aline Lauda Freitas</creator><creator>Muniz, Luciana Vieira</creator><creator>De A. 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Ribeiro, Rosy Iara Maciel</au><au>Dos Santos, Hélio Batista</au><au>Cortes, Vanessa F.</au><au>Soares, Joao Marcos Arantes</au><au>De Lima Santos, Hérica</au><au>Barbosa, Leandro A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tumor Tissue Oxidative Stress Changes and Na, K-ATPase Evaluation in Head and Neck Squamous Cell Carcinoma</atitle><jtitle>The Journal of membrane biology</jtitle><stitle>J Membrane Biol</stitle><addtitle>J Membr Biol</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>254</volume><issue>5-6</issue><spage>475</spage><epage>486</epage><pages>475-486</pages><issn>0022-2631</issn><eissn>1432-1424</eissn><abstract>Changes in metabolism are mechanisms that are largely implicated in the development, progression, and metastasis of head and neck squamous cell carcinoma (HNSCC) and also in resistance to different anticancer therapies. Identification of biomarkers for differentiation between cancerous and normal epithelium, treatment design and prognosis remain a vital issue in the field of head and neck cancer. The present study analyzed the main biochemical changes that occur in HNSCC tumors by through mechanisms involving oxidative stress. The release of substances reactive to thiobarbituric acid was significantly lower in HNSCC tumor tissue as compared to healthy tissue. The assays related to the lipid profile assays showed changes in membrane biophysics of tumor cells due to an increase in total phospholipids and total cholesterol, as well as an increased activity and expression of the α1 subunit of Na, K-ATPase, which is fundamental in the process of carcinogenesis. The modulation of the antioxidant system was also affected, with a decrease in the catalytic activity of the enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), as well as a reduction of glutathione (GSH) content and an increase in H
2
O
2
content. A reduction in catalase (CAT) activity was observed. The data presented here are in accordance with important findings described by us in a previous study, involving the same individuals, but with a focus on the damage generated in red blood cells, resulting from tumor installation. Therefore, it was possible to conclude that the biochemical alterations found in HNSCC cells are fundamental for transformation and maintenance of the tumor cell and once it is installed, it is also capable of generating injuries in the patients' red blood cells. Our data demonstrate that this could be a promising biomarker for HNSCC.
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| ispartof | The Journal of membrane biology, 2021-12, Vol.254 (5-6), p.475-486 |
| issn | 0022-2631 1432-1424 |
| language | eng |
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| subjects | Adenosine Triphosphatases Antioxidants Biochemistry Biomarkers Biomedical and Life Sciences Biophysics Carcinogenesis Carcinogens Catalase Catalytic activity Cholesterol Epithelium Erythrocytes Glutathione Glutathione peroxidase Head & neck cancer Head and neck carcinoma Head and Neck Neoplasms Human Physiology Humans Hydrogen Peroxide Life Sciences Lipids Metastases Na+/K+-exchanging ATPase Na/K-ATPase Ion Transport and Receptor Mediated Signaling Pathways Oxidative Stress Peroxidase Phospholipids Reduction Squamous cell carcinoma Squamous Cell Carcinoma of Head and Neck Superoxide dismutase Thiobarbituric acid Tumor cells Tumors |
| title | Tumor Tissue Oxidative Stress Changes and Na, K-ATPase Evaluation in Head and Neck Squamous Cell Carcinoma |
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