Mycoremediation potential of Aspergillus ochraceus NRRL 3174
Mycoremediation is an important process that targets the removal of petroleum hydrocarbons by fungi. Fungi have advantages with their extensive enzymatic systems, rapid adaptation to toxic organic pollutants, and to adverse environmental conditions. In this study, the colorimetric method was used fo...
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| creator | Bilen Ozyurek, Sezen Avcioglu, Nermin Hande Seyis Bilkay, Isil |
| description | Mycoremediation is an important process that targets the removal of petroleum hydrocarbons by fungi. Fungi have advantages with their extensive enzymatic systems, rapid adaptation to toxic organic pollutants, and to adverse environmental conditions. In this study, the colorimetric method was used for the preliminary investigation of petroleum degradation with ten fungal strains. Petroleum degradation ability of spore suspension, live biomass (fungal pellet and disc) and cell-free culture supernatant of the potent
A. ochraceus
strain were investigated by gravimetric analysis. It was found that the fungal disc (94%) was more successful than the spore suspension (87%) in petroleum degradation under physiological conditions determined as pH:5.0, 1% of petroleum concentration, 5% (v/v) of inoculum concentration (with spore suspension) and 1 g/100 mL of inoculum amount (with fungal disc) and 7 days of the incubation period. The degradation rate constant and half-life period of spore suspension were calculated as 0.291 day
−1
and
t
1/2
= 0.340 and of the fungal disc were 0.401 day
−1
and
t
1/2
= 0.247. Although, 7.5% and 10% (v/v) concentration of cell-free culture supernatant achieved more than 80% petroleum removal, it was not as effective as a fungal disc. According to gas chromatography/mass spectrometry analysis, the fungal disc of
A. ochraceus
strain degraded long-chain
n
-alkanes such as C
35
and C
36
more effectively than
n
-alkanes in the range of C
22
–C
34
. The fact that the
A. ochraceus
NRRL 3174 strain has a high petroleum degradation capacity as well as being a potent biosurfactant producer will provide a different perspective to advanced mycoremediation studies.
Graphic abstract |
| doi_str_mv | 10.1007/s00203-021-02490-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2597012673</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2597012673</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-2599681024a5413c173e9837646f7fcf8f90c0f2dd1bac60bb4291fa07164e8b3</originalsourceid><addsrcrecordid>eNp9kE9LAzEQxYMoWKtfwNOC5-jkz24S8FKKVqEqFAVvIZsmdct2sybbQ7-90RW8eRhmDu-9mfkhdEngmgCImwRAgWGgJBdXgMsjNCGcUQyCvh-jCTCgWCrGTtFZSlsAQqWUE3T7dLAhup1bN2ZoQlf0YXDd0Ji2CL6Ypd7FTdO2-1QE-xGNdXl6Xq2WBSOCn6MTb9rkLn77FL3d373OH_DyZfE4ny2xZSUdMC2VqiTJh5mSE2aJYE5JJipeeeGtl16BBU_Xa1IbW0Fdc6qINyBIxZ2s2RRdjbl9DJ97lwa9DfvY5ZU6Z4v8SyVYVtFRZWNIKTqv-9jsTDxoAvqbkh4p6UxJ_1DSZTax0ZSyuNu4-Bf9j-sLt7BoHA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2597012673</pqid></control><display><type>article</type><title>Mycoremediation potential of Aspergillus ochraceus NRRL 3174</title><source>SpringerLink Journals - AutoHoldings</source><creator>Bilen Ozyurek, Sezen ; Avcioglu, Nermin Hande ; Seyis Bilkay, Isil</creator><creatorcontrib>Bilen Ozyurek, Sezen ; Avcioglu, Nermin Hande ; Seyis Bilkay, Isil</creatorcontrib><description>Mycoremediation is an important process that targets the removal of petroleum hydrocarbons by fungi. Fungi have advantages with their extensive enzymatic systems, rapid adaptation to toxic organic pollutants, and to adverse environmental conditions. In this study, the colorimetric method was used for the preliminary investigation of petroleum degradation with ten fungal strains. Petroleum degradation ability of spore suspension, live biomass (fungal pellet and disc) and cell-free culture supernatant of the potent
A. ochraceus
strain were investigated by gravimetric analysis. It was found that the fungal disc (94%) was more successful than the spore suspension (87%) in petroleum degradation under physiological conditions determined as pH:5.0, 1% of petroleum concentration, 5% (v/v) of inoculum concentration (with spore suspension) and 1 g/100 mL of inoculum amount (with fungal disc) and 7 days of the incubation period. The degradation rate constant and half-life period of spore suspension were calculated as 0.291 day
−1
and
t
1/2
= 0.340 and of the fungal disc were 0.401 day
−1
and
t
1/2
= 0.247. Although, 7.5% and 10% (v/v) concentration of cell-free culture supernatant achieved more than 80% petroleum removal, it was not as effective as a fungal disc. According to gas chromatography/mass spectrometry analysis, the fungal disc of
A. ochraceus
strain degraded long-chain
n
-alkanes such as C
35
and C
36
more effectively than
n
-alkanes in the range of C
22
–C
34
. The fact that the
A. ochraceus
NRRL 3174 strain has a high petroleum degradation capacity as well as being a potent biosurfactant producer will provide a different perspective to advanced mycoremediation studies.
Graphic abstract</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-021-02490-5</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Alkanes ; Archives & records ; Bacteria ; Bioaccumulation ; Biochemistry ; Biodegradation ; Biomedical and Life Sciences ; Bioremediation ; Biotechnology ; Carbon ; Cell Biology ; Cell culture ; Chromatography ; Colorimetry ; Degradation ; Ecology ; Enhanced oil recovery ; Environmental conditions ; Enzymes ; Fungi ; Gas chromatography ; Gravimetric analysis ; Hydrocarbons ; Inoculum ; Investigations ; Life Sciences ; Mass spectrometry ; Mass spectroscopy ; Metabolism ; Microbial Ecology ; Microbiology ; Microorganisms ; Original Paper ; Pesticides ; Petroleum ; Petroleum hydrocarbons ; Physiology ; Pollutants ; Scientific imaging ; Surfactants</subject><ispartof>Archives of microbiology, 2021-12, Vol.203 (10), p.5937-5950</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-2599681024a5413c173e9837646f7fcf8f90c0f2dd1bac60bb4291fa07164e8b3</citedby><cites>FETCH-LOGICAL-c352t-2599681024a5413c173e9837646f7fcf8f90c0f2dd1bac60bb4291fa07164e8b3</cites><orcidid>0000-0002-5056-7051</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-021-02490-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-021-02490-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Bilen Ozyurek, Sezen</creatorcontrib><creatorcontrib>Avcioglu, Nermin Hande</creatorcontrib><creatorcontrib>Seyis Bilkay, Isil</creatorcontrib><title>Mycoremediation potential of Aspergillus ochraceus NRRL 3174</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><description>Mycoremediation is an important process that targets the removal of petroleum hydrocarbons by fungi. Fungi have advantages with their extensive enzymatic systems, rapid adaptation to toxic organic pollutants, and to adverse environmental conditions. In this study, the colorimetric method was used for the preliminary investigation of petroleum degradation with ten fungal strains. Petroleum degradation ability of spore suspension, live biomass (fungal pellet and disc) and cell-free culture supernatant of the potent
A. ochraceus
strain were investigated by gravimetric analysis. It was found that the fungal disc (94%) was more successful than the spore suspension (87%) in petroleum degradation under physiological conditions determined as pH:5.0, 1% of petroleum concentration, 5% (v/v) of inoculum concentration (with spore suspension) and 1 g/100 mL of inoculum amount (with fungal disc) and 7 days of the incubation period. The degradation rate constant and half-life period of spore suspension were calculated as 0.291 day
−1
and
t
1/2
= 0.340 and of the fungal disc were 0.401 day
−1
and
t
1/2
= 0.247. Although, 7.5% and 10% (v/v) concentration of cell-free culture supernatant achieved more than 80% petroleum removal, it was not as effective as a fungal disc. According to gas chromatography/mass spectrometry analysis, the fungal disc of
A. ochraceus
strain degraded long-chain
n
-alkanes such as C
35
and C
36
more effectively than
n
-alkanes in the range of C
22
–C
34
. The fact that the
A. ochraceus
NRRL 3174 strain has a high petroleum degradation capacity as well as being a potent biosurfactant producer will provide a different perspective to advanced mycoremediation studies.
Graphic abstract</description><subject>Alkanes</subject><subject>Archives & records</subject><subject>Bacteria</subject><subject>Bioaccumulation</subject><subject>Biochemistry</subject><subject>Biodegradation</subject><subject>Biomedical and Life Sciences</subject><subject>Bioremediation</subject><subject>Biotechnology</subject><subject>Carbon</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Chromatography</subject><subject>Colorimetry</subject><subject>Degradation</subject><subject>Ecology</subject><subject>Enhanced oil recovery</subject><subject>Environmental conditions</subject><subject>Enzymes</subject><subject>Fungi</subject><subject>Gas chromatography</subject><subject>Gravimetric analysis</subject><subject>Hydrocarbons</subject><subject>Inoculum</subject><subject>Investigations</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolism</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Original Paper</subject><subject>Pesticides</subject><subject>Petroleum</subject><subject>Petroleum hydrocarbons</subject><subject>Physiology</subject><subject>Pollutants</subject><subject>Scientific imaging</subject><subject>Surfactants</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE9LAzEQxYMoWKtfwNOC5-jkz24S8FKKVqEqFAVvIZsmdct2sybbQ7-90RW8eRhmDu-9mfkhdEngmgCImwRAgWGgJBdXgMsjNCGcUQyCvh-jCTCgWCrGTtFZSlsAQqWUE3T7dLAhup1bN2ZoQlf0YXDd0Ji2CL6Ypd7FTdO2-1QE-xGNdXl6Xq2WBSOCn6MTb9rkLn77FL3d373OH_DyZfE4ny2xZSUdMC2VqiTJh5mSE2aJYE5JJipeeeGtl16BBU_Xa1IbW0Fdc6qINyBIxZ2s2RRdjbl9DJ97lwa9DfvY5ZU6Z4v8SyVYVtFRZWNIKTqv-9jsTDxoAvqbkh4p6UxJ_1DSZTax0ZSyuNu4-Bf9j-sLt7BoHA</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Bilen Ozyurek, Sezen</creator><creator>Avcioglu, Nermin Hande</creator><creator>Seyis Bilkay, Isil</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature 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potential of Aspergillus ochraceus NRRL 3174</title><author>Bilen Ozyurek, Sezen ; Avcioglu, Nermin Hande ; Seyis Bilkay, Isil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-2599681024a5413c173e9837646f7fcf8f90c0f2dd1bac60bb4291fa07164e8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alkanes</topic><topic>Archives & records</topic><topic>Bacteria</topic><topic>Bioaccumulation</topic><topic>Biochemistry</topic><topic>Biodegradation</topic><topic>Biomedical and Life Sciences</topic><topic>Bioremediation</topic><topic>Biotechnology</topic><topic>Carbon</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Chromatography</topic><topic>Colorimetry</topic><topic>Degradation</topic><topic>Ecology</topic><topic>Enhanced oil recovery</topic><topic>Environmental conditions</topic><topic>Enzymes</topic><topic>Fungi</topic><topic>Gas chromatography</topic><topic>Gravimetric analysis</topic><topic>Hydrocarbons</topic><topic>Inoculum</topic><topic>Investigations</topic><topic>Life Sciences</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolism</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Original Paper</topic><topic>Pesticides</topic><topic>Petroleum</topic><topic>Petroleum hydrocarbons</topic><topic>Physiology</topic><topic>Pollutants</topic><topic>Scientific imaging</topic><topic>Surfactants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bilen Ozyurek, Sezen</creatorcontrib><creatorcontrib>Avcioglu, Nermin Hande</creatorcontrib><creatorcontrib>Seyis Bilkay, Isil</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS 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of microbiology</jtitle><stitle>Arch Microbiol</stitle><date>2021-12-01</date><risdate>2021</risdate><volume>203</volume><issue>10</issue><spage>5937</spage><epage>5950</epage><pages>5937-5950</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Mycoremediation is an important process that targets the removal of petroleum hydrocarbons by fungi. Fungi have advantages with their extensive enzymatic systems, rapid adaptation to toxic organic pollutants, and to adverse environmental conditions. In this study, the colorimetric method was used for the preliminary investigation of petroleum degradation with ten fungal strains. Petroleum degradation ability of spore suspension, live biomass (fungal pellet and disc) and cell-free culture supernatant of the potent
A. ochraceus
strain were investigated by gravimetric analysis. It was found that the fungal disc (94%) was more successful than the spore suspension (87%) in petroleum degradation under physiological conditions determined as pH:5.0, 1% of petroleum concentration, 5% (v/v) of inoculum concentration (with spore suspension) and 1 g/100 mL of inoculum amount (with fungal disc) and 7 days of the incubation period. The degradation rate constant and half-life period of spore suspension were calculated as 0.291 day
−1
and
t
1/2
= 0.340 and of the fungal disc were 0.401 day
−1
and
t
1/2
= 0.247. Although, 7.5% and 10% (v/v) concentration of cell-free culture supernatant achieved more than 80% petroleum removal, it was not as effective as a fungal disc. According to gas chromatography/mass spectrometry analysis, the fungal disc of
A. ochraceus
strain degraded long-chain
n
-alkanes such as C
35
and C
36
more effectively than
n
-alkanes in the range of C
22
–C
34
. The fact that the
A. ochraceus
NRRL 3174 strain has a high petroleum degradation capacity as well as being a potent biosurfactant producer will provide a different perspective to advanced mycoremediation studies.
Graphic abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00203-021-02490-5</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-5056-7051</orcidid></addata></record> |
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| subjects | Alkanes Archives & records Bacteria Bioaccumulation Biochemistry Biodegradation Biomedical and Life Sciences Bioremediation Biotechnology Carbon Cell Biology Cell culture Chromatography Colorimetry Degradation Ecology Enhanced oil recovery Environmental conditions Enzymes Fungi Gas chromatography Gravimetric analysis Hydrocarbons Inoculum Investigations Life Sciences Mass spectrometry Mass spectroscopy Metabolism Microbial Ecology Microbiology Microorganisms Original Paper Pesticides Petroleum Petroleum hydrocarbons Physiology Pollutants Scientific imaging Surfactants |
| title | Mycoremediation potential of Aspergillus ochraceus NRRL 3174 |
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