A versatile CRISPR/Cas12a-based sensitivity amplifier suitable for commercial HRP-based ELISA kits

•A CRISPR/Cas12a-based recipe for a broadly applicable ELISA sensitivity amplification.•Capable of integrated into commercial HRP-based ELISA kit without changing the original reagents or setup.•A possible solution to rapidly deliver a user-friendly and affordable sensitivity amplifier to end-users....

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Sensors and actuators. B, Chemical Chemical, 2021-11, Vol.347, p.130533, Article 130533
Hauptverfasser:	Li, Yi, Deng, Fei, Goldys, Ewa M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplifiers Antibodies Antibody Cas12a CRISPR CRISPR/Cas ELISA Fluorescence Immunoassay Nuclease Nucleic acids Peroxidase Reagents Sensitivity Signal amplification
Online-Zugang:	Volltext
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