Exonuclease III-assisted nucleic acid amplification fluorescence immunoassay for the ultrasensitive detection of chloramphenicol in milk

•ExoⅢ assisted signal amplification was utilized for ultrasensitive immunoassay.•This method realizes 0.001 ng/L chloramphenicol detection in skim milk.•Au nanoparticles modified with antibodies and DNAs are used as bifunctional probes. Herein, an integrated approach based on immunoassay and exonucl...

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Veröffentlicht in:	Sensors and actuators. B, Chemical Chemical, 2021-11, Vol.347, p.130564, Article 130564
Hauptverfasser:	Sang, Panting, Hu, Zhigang, Cheng, Yuliang, Yu, Hang, Yang, Fangwei, Xie, Yunfei, Yao, Weirong, Guo, Yahui, Qian, He
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Antibiotics detection Antibodies Chloramphenicol Chloromycetin Exonuclease III Fluorescence Gentamicin Gold Immunoassay Integrated approach Isothermal amplification Nanoparticles Nucleic acids Streptomycin
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