Exonuclease III-assisted nucleic acid amplification fluorescence immunoassay for the ultrasensitive detection of chloramphenicol in milk
•ExoⅢ assisted signal amplification was utilized for ultrasensitive immunoassay.•This method realizes 0.001 ng/L chloramphenicol detection in skim milk.•Au nanoparticles modified with antibodies and DNAs are used as bifunctional probes. Herein, an integrated approach based on immunoassay and exonucl...
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| Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2021-11, Vol.347, p.130564, Article 130564 |
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| container_title | Sensors and actuators. B, Chemical |
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| creator | Sang, Panting Hu, Zhigang Cheng, Yuliang Yu, Hang Yang, Fangwei Xie, Yunfei Yao, Weirong Guo, Yahui Qian, He |
| description | •ExoⅢ assisted signal amplification was utilized for ultrasensitive immunoassay.•This method realizes 0.001 ng/L chloramphenicol detection in skim milk.•Au nanoparticles modified with antibodies and DNAs are used as bifunctional probes.
Herein, an integrated approach based on immunoassay and exonuclease III-assisted nucleic acid amplification (EANA), which is defined as exonuclease III-assisted nucleic acid amplification fluorescence immunoassay (EA-NAFIA) was developed for the detection of chloramphenicol (CAP). Au nanoparticles (AuNPs) modified with antibody and DNA were used as bifunctional probe, in which antibody captured the target, and DNA initiated the signal amplification through EANA. A detection linear range from 0.01 to 5 ng/L was obtained under optimized conditions, and the detection limit was as low as 0.001 ng/L. There was no cross reaction with streptomycin, kanamycin, gentamicin, thiamphenicol. This assay was successfully applied to detect CAP in skim milk, with acceptable recoveries. This kind of integrated strategy demonstrated the excellent hybrid performance of indirect immunoassay with ultrasensitive sensitivity. |
| doi_str_mv | 10.1016/j.snb.2021.130564 |
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Herein, an integrated approach based on immunoassay and exonuclease III-assisted nucleic acid amplification (EANA), which is defined as exonuclease III-assisted nucleic acid amplification fluorescence immunoassay (EA-NAFIA) was developed for the detection of chloramphenicol (CAP). Au nanoparticles (AuNPs) modified with antibody and DNA were used as bifunctional probe, in which antibody captured the target, and DNA initiated the signal amplification through EANA. A detection linear range from 0.01 to 5 ng/L was obtained under optimized conditions, and the detection limit was as low as 0.001 ng/L. There was no cross reaction with streptomycin, kanamycin, gentamicin, thiamphenicol. This assay was successfully applied to detect CAP in skim milk, with acceptable recoveries. This kind of integrated strategy demonstrated the excellent hybrid performance of indirect immunoassay with ultrasensitive sensitivity.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2021.130564</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Amplification ; Antibiotics detection ; Antibodies ; Chloramphenicol ; Chloromycetin ; Exonuclease III ; Fluorescence ; Gentamicin ; Gold ; Immunoassay ; Integrated approach ; Isothermal amplification ; Nanoparticles ; Nucleic acids ; Streptomycin</subject><ispartof>Sensors and actuators. B, Chemical, 2021-11, Vol.347, p.130564, Article 130564</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright Elsevier Science Ltd. Nov 15, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c325t-cd58e07c72eac49ec63251e86c8f64414d77eabdba19909dd0a56641b021913e3</citedby><cites>FETCH-LOGICAL-c325t-cd58e07c72eac49ec63251e86c8f64414d77eabdba19909dd0a56641b021913e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.snb.2021.130564$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids></links><search><creatorcontrib>Sang, Panting</creatorcontrib><creatorcontrib>Hu, Zhigang</creatorcontrib><creatorcontrib>Cheng, Yuliang</creatorcontrib><creatorcontrib>Yu, Hang</creatorcontrib><creatorcontrib>Yang, Fangwei</creatorcontrib><creatorcontrib>Xie, Yunfei</creatorcontrib><creatorcontrib>Yao, Weirong</creatorcontrib><creatorcontrib>Guo, Yahui</creatorcontrib><creatorcontrib>Qian, He</creatorcontrib><title>Exonuclease III-assisted nucleic acid amplification fluorescence immunoassay for the ultrasensitive detection of chloramphenicol in milk</title><title>Sensors and actuators. B, Chemical</title><description>•ExoⅢ assisted signal amplification was utilized for ultrasensitive immunoassay.•This method realizes 0.001 ng/L chloramphenicol detection in skim milk.•Au nanoparticles modified with antibodies and DNAs are used as bifunctional probes.
Herein, an integrated approach based on immunoassay and exonuclease III-assisted nucleic acid amplification (EANA), which is defined as exonuclease III-assisted nucleic acid amplification fluorescence immunoassay (EA-NAFIA) was developed for the detection of chloramphenicol (CAP). Au nanoparticles (AuNPs) modified with antibody and DNA were used as bifunctional probe, in which antibody captured the target, and DNA initiated the signal amplification through EANA. A detection linear range from 0.01 to 5 ng/L was obtained under optimized conditions, and the detection limit was as low as 0.001 ng/L. There was no cross reaction with streptomycin, kanamycin, gentamicin, thiamphenicol. This assay was successfully applied to detect CAP in skim milk, with acceptable recoveries. This kind of integrated strategy demonstrated the excellent hybrid performance of indirect immunoassay with ultrasensitive sensitivity.</description><subject>Amplification</subject><subject>Antibiotics detection</subject><subject>Antibodies</subject><subject>Chloramphenicol</subject><subject>Chloromycetin</subject><subject>Exonuclease III</subject><subject>Fluorescence</subject><subject>Gentamicin</subject><subject>Gold</subject><subject>Immunoassay</subject><subject>Integrated approach</subject><subject>Isothermal amplification</subject><subject>Nanoparticles</subject><subject>Nucleic acids</subject><subject>Streptomycin</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE1PwzAMhiMEEuPjB3CLxLkjadp0FSeE-JiExAXOUea4mkebjKRF8A_42WSMMydLlp_X9sPYhRRzKaS-2syTX81LUcq5VKLW1QGbyUWjCiWa5pDNRFvWRSVEfcxOUtoIISqlxYx9330GP0GPNiFfLpeFTYnSiI7_dgm4BXLcDtueOgI7UvC866cQMQF6QE7DMPmQMfvFuxD5uEY-9WPMgT7RSB_IHY4Iv2ToOKz7EHPeGj1B6Dl5PlD_dsaOOtsnPP-rp-z1_u7l9rF4en5Y3t48FaDKeizA1QsUDTQlWqhaBJ3bEhcaFp2uKlm5pkG7cisr21a0zglba13JVTbTSoXqlF3uc7cxvE-YRrMJU_R5pSnrVmmt2lLlKbmfghhSitiZbaTBxi8jhdkJNxuThZudcLMXnpnrPYP5_A_CaBLQTpGjmN83LtA_9A9zeYvX</recordid><startdate>20211115</startdate><enddate>20211115</enddate><creator>Sang, Panting</creator><creator>Hu, Zhigang</creator><creator>Cheng, Yuliang</creator><creator>Yu, Hang</creator><creator>Yang, Fangwei</creator><creator>Xie, Yunfei</creator><creator>Yao, Weirong</creator><creator>Guo, Yahui</creator><creator>Qian, He</creator><general>Elsevier B.V</general><general>Elsevier Science Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20211115</creationdate><title>Exonuclease III-assisted nucleic acid amplification fluorescence immunoassay for the ultrasensitive detection of chloramphenicol in milk</title><author>Sang, Panting ; Hu, Zhigang ; Cheng, Yuliang ; Yu, Hang ; Yang, Fangwei ; Xie, Yunfei ; Yao, Weirong ; Guo, Yahui ; Qian, He</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-cd58e07c72eac49ec63251e86c8f64414d77eabdba19909dd0a56641b021913e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amplification</topic><topic>Antibiotics detection</topic><topic>Antibodies</topic><topic>Chloramphenicol</topic><topic>Chloromycetin</topic><topic>Exonuclease III</topic><topic>Fluorescence</topic><topic>Gentamicin</topic><topic>Gold</topic><topic>Immunoassay</topic><topic>Integrated approach</topic><topic>Isothermal amplification</topic><topic>Nanoparticles</topic><topic>Nucleic acids</topic><topic>Streptomycin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sang, Panting</creatorcontrib><creatorcontrib>Hu, Zhigang</creatorcontrib><creatorcontrib>Cheng, Yuliang</creatorcontrib><creatorcontrib>Yu, Hang</creatorcontrib><creatorcontrib>Yang, Fangwei</creatorcontrib><creatorcontrib>Xie, Yunfei</creatorcontrib><creatorcontrib>Yao, Weirong</creatorcontrib><creatorcontrib>Guo, Yahui</creatorcontrib><creatorcontrib>Qian, He</creatorcontrib><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sang, Panting</au><au>Hu, Zhigang</au><au>Cheng, Yuliang</au><au>Yu, Hang</au><au>Yang, Fangwei</au><au>Xie, Yunfei</au><au>Yao, Weirong</au><au>Guo, Yahui</au><au>Qian, He</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exonuclease III-assisted nucleic acid amplification fluorescence immunoassay for the ultrasensitive detection of chloramphenicol in milk</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2021-11-15</date><risdate>2021</risdate><volume>347</volume><spage>130564</spage><pages>130564-</pages><artnum>130564</artnum><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>•ExoⅢ assisted signal amplification was utilized for ultrasensitive immunoassay.•This method realizes 0.001 ng/L chloramphenicol detection in skim milk.•Au nanoparticles modified with antibodies and DNAs are used as bifunctional probes.
Herein, an integrated approach based on immunoassay and exonuclease III-assisted nucleic acid amplification (EANA), which is defined as exonuclease III-assisted nucleic acid amplification fluorescence immunoassay (EA-NAFIA) was developed for the detection of chloramphenicol (CAP). Au nanoparticles (AuNPs) modified with antibody and DNA were used as bifunctional probe, in which antibody captured the target, and DNA initiated the signal amplification through EANA. A detection linear range from 0.01 to 5 ng/L was obtained under optimized conditions, and the detection limit was as low as 0.001 ng/L. There was no cross reaction with streptomycin, kanamycin, gentamicin, thiamphenicol. This assay was successfully applied to detect CAP in skim milk, with acceptable recoveries. This kind of integrated strategy demonstrated the excellent hybrid performance of indirect immunoassay with ultrasensitive sensitivity.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2021.130564</doi></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Amplification Antibiotics detection Antibodies Chloramphenicol Chloromycetin Exonuclease III Fluorescence Gentamicin Gold Immunoassay Integrated approach Isothermal amplification Nanoparticles Nucleic acids Streptomycin |
| title | Exonuclease III-assisted nucleic acid amplification fluorescence immunoassay for the ultrasensitive detection of chloramphenicol in milk |
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