On the interaction of lytic polysaccharide monooxygenases (LPMOs) with phosphoric acid-swollen cellulose (PASC)
•MtLPMO9H is immobilized and direct electron transfer is achieved.•The interaction of MtLPMO9H with PASC is studied with FTacV.•The kinetic model for the interaction of MtLPMO9H with PASC with FTacV is developed.•The kinetic constant for the interaction of MtLPMO9H with PASC is extracted. In this wo...
Gespeichert in:
| Veröffentlicht in: | Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2021-09, Vol.897, p.115540, Article 115540 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 115540 |
| container_title | Journal of electroanalytical chemistry (Lausanne, Switzerland) |
| container_volume | 897 |
| creator | Zouraris, D. Karnaouri, A. Pandis, P.K. Argirusis, Chr Topakas, E. Karantonis, A. |
| description | •MtLPMO9H is immobilized and direct electron transfer is achieved.•The interaction of MtLPMO9H with PASC is studied with FTacV.•The kinetic model for the interaction of MtLPMO9H with PASC with FTacV is developed.•The kinetic constant for the interaction of MtLPMO9H with PASC is extracted.
In this work, a lytic polysaccharide monooxygenase (LPMO) belonging to the AA9 family from the filamentous fungus Thermothelomyces thermophilus, MtLPMO9H, is immobilized on cobalt functionalized multi-walled carbon nanotubes modified glassy carbon electrode. The step of the binding of phosphoric acid swollen cellulose on this LPMO is studied using large amplitude fast Fourier transform alternating current voltammetry (FTacV) at different temperatures, extracting the corresponding kinetic constants, based on a model developed for the interaction of immobilized enzyme with free, in the solution, species. |
| doi_str_mv | 10.1016/j.jelechem.2021.115540 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2593194310</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S157266572100566X</els_id><sourcerecordid>2593194310</sourcerecordid><originalsourceid>FETCH-LOGICAL-c340t-357ce2366776c5e57c1e063612684edf2f6d7d3ace14b10bbea4d01dacc5f2bb3</originalsourceid><addsrcrecordid>eNqFkE1LAzEQhhdRsFb_ggS8tIet-dhk25ul-AWVFtRzyCazbpZ0U5Ot2n9vpHr2MMwMvO98PFl2SfCEYCKu20kLDnQDmwnFlEwI4bzAR9mATEuWUy5mx6nmJc2F4OVpdhZjizGdTgkdZH7Vob4BZLsegtK99R3yNXL73mq09W4fldaNCtYA2vjO-6_9G3QqQkSj5fppFcfo0_YN2jY-pgjJpbQ1efz0zkGHNDi3cz4CGq3nz4vxeXZSKxfh4jcPs9e725fFQ75c3T8u5stcswL3OeOlBsqEKEuhOaSOABZMECqmBZia1sKUhikNpKgIripQhcHEpGN5TauKDbOrw9xt8O87iL1s_S50aaWkfMbIrGAEJ5U4qHTwMQao5TbYjQp7SbD8gStb-QdX_sCVB7jJeHMwQvrhw0KQUVvoNBgbQPfSePvfiG9O94dA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2593194310</pqid></control><display><type>article</type><title>On the interaction of lytic polysaccharide monooxygenases (LPMOs) with phosphoric acid-swollen cellulose (PASC)</title><source>ScienceDirect Journals (5 years ago - present)</source><creator>Zouraris, D. ; Karnaouri, A. ; Pandis, P.K. ; Argirusis, Chr ; Topakas, E. ; Karantonis, A.</creator><creatorcontrib>Zouraris, D. ; Karnaouri, A. ; Pandis, P.K. ; Argirusis, Chr ; Topakas, E. ; Karantonis, A.</creatorcontrib><description>•MtLPMO9H is immobilized and direct electron transfer is achieved.•The interaction of MtLPMO9H with PASC is studied with FTacV.•The kinetic model for the interaction of MtLPMO9H with PASC with FTacV is developed.•The kinetic constant for the interaction of MtLPMO9H with PASC is extracted.
In this work, a lytic polysaccharide monooxygenase (LPMO) belonging to the AA9 family from the filamentous fungus Thermothelomyces thermophilus, MtLPMO9H, is immobilized on cobalt functionalized multi-walled carbon nanotubes modified glassy carbon electrode. The step of the binding of phosphoric acid swollen cellulose on this LPMO is studied using large amplitude fast Fourier transform alternating current voltammetry (FTacV) at different temperatures, extracting the corresponding kinetic constants, based on a model developed for the interaction of immobilized enzyme with free, in the solution, species.</description><identifier>ISSN: 1572-6657</identifier><identifier>EISSN: 1873-2569</identifier><identifier>DOI: 10.1016/j.jelechem.2021.115540</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Cellulose ; Cyclic voltammetry ; Fast Fourier transformations ; Fourier transform ac voltammetry ; Fourier transforms ; Glassy carbon ; LPMO ; Multi wall carbon nanotubes ; PASC ; Phosphoric acid ; Polysaccharides</subject><ispartof>Journal of electroanalytical chemistry (Lausanne, Switzerland), 2021-09, Vol.897, p.115540, Article 115540</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright Elsevier Science Ltd. Sep 15, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-357ce2366776c5e57c1e063612684edf2f6d7d3ace14b10bbea4d01dacc5f2bb3</citedby><cites>FETCH-LOGICAL-c340t-357ce2366776c5e57c1e063612684edf2f6d7d3ace14b10bbea4d01dacc5f2bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jelechem.2021.115540$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids></links><search><creatorcontrib>Zouraris, D.</creatorcontrib><creatorcontrib>Karnaouri, A.</creatorcontrib><creatorcontrib>Pandis, P.K.</creatorcontrib><creatorcontrib>Argirusis, Chr</creatorcontrib><creatorcontrib>Topakas, E.</creatorcontrib><creatorcontrib>Karantonis, A.</creatorcontrib><title>On the interaction of lytic polysaccharide monooxygenases (LPMOs) with phosphoric acid-swollen cellulose (PASC)</title><title>Journal of electroanalytical chemistry (Lausanne, Switzerland)</title><description>•MtLPMO9H is immobilized and direct electron transfer is achieved.•The interaction of MtLPMO9H with PASC is studied with FTacV.•The kinetic model for the interaction of MtLPMO9H with PASC with FTacV is developed.•The kinetic constant for the interaction of MtLPMO9H with PASC is extracted.
In this work, a lytic polysaccharide monooxygenase (LPMO) belonging to the AA9 family from the filamentous fungus Thermothelomyces thermophilus, MtLPMO9H, is immobilized on cobalt functionalized multi-walled carbon nanotubes modified glassy carbon electrode. The step of the binding of phosphoric acid swollen cellulose on this LPMO is studied using large amplitude fast Fourier transform alternating current voltammetry (FTacV) at different temperatures, extracting the corresponding kinetic constants, based on a model developed for the interaction of immobilized enzyme with free, in the solution, species.</description><subject>Cellulose</subject><subject>Cyclic voltammetry</subject><subject>Fast Fourier transformations</subject><subject>Fourier transform ac voltammetry</subject><subject>Fourier transforms</subject><subject>Glassy carbon</subject><subject>LPMO</subject><subject>Multi wall carbon nanotubes</subject><subject>PASC</subject><subject>Phosphoric acid</subject><subject>Polysaccharides</subject><issn>1572-6657</issn><issn>1873-2569</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LAzEQhhdRsFb_ggS8tIet-dhk25ul-AWVFtRzyCazbpZ0U5Ot2n9vpHr2MMwMvO98PFl2SfCEYCKu20kLDnQDmwnFlEwI4bzAR9mATEuWUy5mx6nmJc2F4OVpdhZjizGdTgkdZH7Vob4BZLsegtK99R3yNXL73mq09W4fldaNCtYA2vjO-6_9G3QqQkSj5fppFcfo0_YN2jY-pgjJpbQ1efz0zkGHNDi3cz4CGq3nz4vxeXZSKxfh4jcPs9e725fFQ75c3T8u5stcswL3OeOlBsqEKEuhOaSOABZMECqmBZia1sKUhikNpKgIripQhcHEpGN5TauKDbOrw9xt8O87iL1s_S50aaWkfMbIrGAEJ5U4qHTwMQao5TbYjQp7SbD8gStb-QdX_sCVB7jJeHMwQvrhw0KQUVvoNBgbQPfSePvfiG9O94dA</recordid><startdate>20210915</startdate><enddate>20210915</enddate><creator>Zouraris, D.</creator><creator>Karnaouri, A.</creator><creator>Pandis, P.K.</creator><creator>Argirusis, Chr</creator><creator>Topakas, E.</creator><creator>Karantonis, A.</creator><general>Elsevier B.V</general><general>Elsevier Science Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20210915</creationdate><title>On the interaction of lytic polysaccharide monooxygenases (LPMOs) with phosphoric acid-swollen cellulose (PASC)</title><author>Zouraris, D. ; Karnaouri, A. ; Pandis, P.K. ; Argirusis, Chr ; Topakas, E. ; Karantonis, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-357ce2366776c5e57c1e063612684edf2f6d7d3ace14b10bbea4d01dacc5f2bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cellulose</topic><topic>Cyclic voltammetry</topic><topic>Fast Fourier transformations</topic><topic>Fourier transform ac voltammetry</topic><topic>Fourier transforms</topic><topic>Glassy carbon</topic><topic>LPMO</topic><topic>Multi wall carbon nanotubes</topic><topic>PASC</topic><topic>Phosphoric acid</topic><topic>Polysaccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zouraris, D.</creatorcontrib><creatorcontrib>Karnaouri, A.</creatorcontrib><creatorcontrib>Pandis, P.K.</creatorcontrib><creatorcontrib>Argirusis, Chr</creatorcontrib><creatorcontrib>Topakas, E.</creatorcontrib><creatorcontrib>Karantonis, A.</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Journal of electroanalytical chemistry (Lausanne, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zouraris, D.</au><au>Karnaouri, A.</au><au>Pandis, P.K.</au><au>Argirusis, Chr</au><au>Topakas, E.</au><au>Karantonis, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>On the interaction of lytic polysaccharide monooxygenases (LPMOs) with phosphoric acid-swollen cellulose (PASC)</atitle><jtitle>Journal of electroanalytical chemistry (Lausanne, Switzerland)</jtitle><date>2021-09-15</date><risdate>2021</risdate><volume>897</volume><spage>115540</spage><pages>115540-</pages><artnum>115540</artnum><issn>1572-6657</issn><eissn>1873-2569</eissn><abstract>•MtLPMO9H is immobilized and direct electron transfer is achieved.•The interaction of MtLPMO9H with PASC is studied with FTacV.•The kinetic model for the interaction of MtLPMO9H with PASC with FTacV is developed.•The kinetic constant for the interaction of MtLPMO9H with PASC is extracted.
In this work, a lytic polysaccharide monooxygenase (LPMO) belonging to the AA9 family from the filamentous fungus Thermothelomyces thermophilus, MtLPMO9H, is immobilized on cobalt functionalized multi-walled carbon nanotubes modified glassy carbon electrode. The step of the binding of phosphoric acid swollen cellulose on this LPMO is studied using large amplitude fast Fourier transform alternating current voltammetry (FTacV) at different temperatures, extracting the corresponding kinetic constants, based on a model developed for the interaction of immobilized enzyme with free, in the solution, species.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.jelechem.2021.115540</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1572-6657 |
| ispartof | Journal of electroanalytical chemistry (Lausanne, Switzerland), 2021-09, Vol.897, p.115540, Article 115540 |
| issn | 1572-6657 1873-2569 |
| language | eng |
| recordid | cdi_proquest_journals_2593194310 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Cellulose Cyclic voltammetry Fast Fourier transformations Fourier transform ac voltammetry Fourier transforms Glassy carbon LPMO Multi wall carbon nanotubes PASC Phosphoric acid Polysaccharides |
| title | On the interaction of lytic polysaccharide monooxygenases (LPMOs) with phosphoric acid-swollen cellulose (PASC) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T06%3A01%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=On%20the%20interaction%20of%20lytic%20polysaccharide%20monooxygenases%20(LPMOs)%20with%20phosphoric%20acid-swollen%20cellulose%20(PASC)&rft.jtitle=Journal%20of%20electroanalytical%20chemistry%20(Lausanne,%20Switzerland)&rft.au=Zouraris,%20D.&rft.date=2021-09-15&rft.volume=897&rft.spage=115540&rft.pages=115540-&rft.artnum=115540&rft.issn=1572-6657&rft.eissn=1873-2569&rft_id=info:doi/10.1016/j.jelechem.2021.115540&rft_dat=%3Cproquest_cross%3E2593194310%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2593194310&rft_id=info:pmid/&rft_els_id=S157266572100566X&rfr_iscdi=true |