The influence of medium composition on the microbial secretory production of hydroxyalkanoate oligomers

With the aid of a chain transfer (CT) reaction, hydroxyalkanoate (HA) oligomers can be secreted by recombinant Escherichia coli carrying the gene encoding a lactate-polymerizing enzyme (PhaC1PsSTQK) in Luria-Bertani (LB) medium supplemented with a carbon source and CT agent. In this study, HA oligom...

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Veröffentlicht in:	Journal of general and applied microbiology 2021, Vol.67(4), pp.134-141
Hauptverfasser:	Mizuno, Shoji, Sakurai, Tetsuo, Nabasama, Mikito, Kawakami, Kyouhei, Hiroe, Ayaka, Taguchi, Seiichi, Tsuge, Takeharu
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbon sources Chain transfer chain transfer reaction CT agent Culture Media - analysis E coli Escherichia coli Escherichia coli - chemistry Escherichia coli - metabolism Hydroxyapatite Lactic acid Microorganisms Microorganisms, Genetically-Modified - chemistry Microorganisms, Genetically-Modified - metabolism Nutrients Oligomers Osmosis Osmotic pressure PHA synthase polyhydroxyalkanoate Polyhydroxyalkanoates - biosynthesis Polymerization Polymers Secretion Sodium chloride Supplements Yeast yeast extract Yeasts
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