Putative virulence factors of Candida species colonising asymptomatic pregnant Jordanian women
Objectives: Pregnant women are frequently exposed to yeast colonisation and infection compared to non-pregnant women. This study was undertaken to investigate carriage rates of Candida species and their virulence factors in high vaginal samples from asymptomatic pregnant women as a probable predispo...
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| Veröffentlicht in: | New Zealand journal of medical laboratory science 2021-04, Vol.75 (1), p.51-56 |
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| creator | Abu-Lubad, Mohammad A Helaly, Ghada F Aqel, Amin A Jarajreh, Duaa A Kharabsheh, Ahlam M. Al Abufraijeh, Seham M Albataineh, Eman M Alnawaiseh, Nedal A |
| description | Objectives: Pregnant women are frequently exposed to yeast colonisation and infection compared to non-pregnant women. This study was undertaken to investigate carriage rates of Candida species and their virulence factors in high vaginal samples from asymptomatic pregnant women as a probable predisposing factor for neonatal candidiasis.
Methods: High vaginal swabs were collected from 200 pregnant women from Al-Karak Governmental Hospital, Jordan from March to December 2018. Candida isolates were identified by their growth on CHROMagar Candida and Vitek2 automated system. Some virulence factors were determined.
Results: Among 200 swabs tested, 67 (33.5%) yielded Candida isolates as follows: C. ablicans, 28 (41.8%), while non-albicans Candida (NAC) were 39 (58.2%) isolates (p=0.04). C. parapsilosis was the most prevailed NAC species isolated, 29 (74.4%) followed by C. tropicalis: 6 (15.4%) while C. glabrata and C. krusei, each represented 2 (5.1%). Among C. albicans isolates, 27 (96.4%), 18 (64.3%), and 24 (85.7%) compared to 24 (82.7%), 4 (13.8%), and 25 (86.2%) among C. parapsilosis were positive for proteinase, phospholipase, and haemolysin respectively. The protease activity was the highest detected (88.1% of Candida isolates) compared to other enzymes but did not reach statistical significance between Candida species (p=0.215). Phospholipase activity was significantly detected among C. albicans isolates compared to other species (p |
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| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2587189223</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A660990371</galeid><informt_id>10.3316/informit.722251250071139</informt_id><sourcerecordid>A660990371</sourcerecordid><originalsourceid>FETCH-LOGICAL-g242t-359387a12b6fe20dc43ab26edf7a7d35540fe13159ef23ad842018b56f6cabff3</originalsourceid><addsrcrecordid>eNptkFtLAzEQhfdBwVr9DwGfK7ls9vJYilcK-lBfDdNksqbsJjVJK_57l1YogszDwJzvnBnmrJgwVrMZZa28KC5T2lBaVkK2k-L9dZchuz2SvYu7Hr1GYkHnEBMJlizAG2eApC1qh4no0AfvkvMdgfQ9bHMYRrcm24idB5_Jc4gGvANPvsKA_qo4t9AnvP7t0-Lt_m61eJwtXx6eFvPlrOMlz7PxFNHUwPi6ssip0aWANa_Q2BpqI6QsqUUmmGzRcgGmKTllzVpWttKwtlZMi5tj7jaGzx2mrDZhF_24UnHZ1KxpORcnqoMelfM25Ah6cEmreVXRtqWiZiN1-w81lsHB6eDRunH-x7A6GuLgshpf1KPOLvi0gZxUQoj645Bz0EPslAlOMaqEYNVJqDnnknFJac2YaMUPfCuJqQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2587189223</pqid></control><display><type>article</type><title>Putative virulence factors of Candida species colonising asymptomatic pregnant Jordanian women</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Abu-Lubad, Mohammad A ; Helaly, Ghada F ; Aqel, Amin A ; Jarajreh, Duaa A ; Kharabsheh, Ahlam M. Al ; Abufraijeh, Seham M ; Albataineh, Eman M ; Alnawaiseh, Nedal A</creator><creatorcontrib>Abu-Lubad, Mohammad A ; Helaly, Ghada F ; Aqel, Amin A ; Jarajreh, Duaa A ; Kharabsheh, Ahlam M. Al ; Abufraijeh, Seham M ; Albataineh, Eman M ; Alnawaiseh, Nedal A</creatorcontrib><description>Objectives: Pregnant women are frequently exposed to yeast colonisation and infection compared to non-pregnant women. This study was undertaken to investigate carriage rates of Candida species and their virulence factors in high vaginal samples from asymptomatic pregnant women as a probable predisposing factor for neonatal candidiasis.
Methods: High vaginal swabs were collected from 200 pregnant women from Al-Karak Governmental Hospital, Jordan from March to December 2018. Candida isolates were identified by their growth on CHROMagar Candida and Vitek2 automated system. Some virulence factors were determined.
Results: Among 200 swabs tested, 67 (33.5%) yielded Candida isolates as follows: C. ablicans, 28 (41.8%), while non-albicans Candida (NAC) were 39 (58.2%) isolates (p=0.04). C. parapsilosis was the most prevailed NAC species isolated, 29 (74.4%) followed by C. tropicalis: 6 (15.4%) while C. glabrata and C. krusei, each represented 2 (5.1%). Among C. albicans isolates, 27 (96.4%), 18 (64.3%), and 24 (85.7%) compared to 24 (82.7%), 4 (13.8%), and 25 (86.2%) among C. parapsilosis were positive for proteinase, phospholipase, and haemolysin respectively. The protease activity was the highest detected (88.1% of Candida isolates) compared to other enzymes but did not reach statistical significance between Candida species (p=0.215). Phospholipase activity was significantly detected among C. albicans isolates compared to other species (p <0.001). Insignificant differences in haemolysin production was observed among isolates (p= 0.134).
Conclusions: In Jordanian pregnant women, NAC, mainly C. parapsilosis, were the most frequently isolated Candida species from high vaginal swabs. Routine screening and treatment are recommended for pregnant women, irrespective of symptoms.</description><identifier>ISSN: 1171-0195</identifier><language>eng</language><publisher>Auckland: New Zealand Institute of Medical Laboratory Science</publisher><subject>Asymptomatic ; Automation ; Candida albicans ; Cultures and culture media ; Diabetes ; Evaluation ; Health aspects ; Health care ; Infection ; Infections ; Morphology ; Mycoses ; Phospholipases ; Pregnancy ; Pregnant women ; Vagina ; Virulence ; Womens health ; Yeast fungi</subject><ispartof>New Zealand journal of medical laboratory science, 2021-04, Vol.75 (1), p.51-56</ispartof><rights>COPYRIGHT 2021 New Zealand Institute of Medical Laboratory Science</rights><rights>Copyright New Zealand Institute of Medical Laboratory Science Apr 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785</link.rule.ids></links><search><creatorcontrib>Abu-Lubad, Mohammad A</creatorcontrib><creatorcontrib>Helaly, Ghada F</creatorcontrib><creatorcontrib>Aqel, Amin A</creatorcontrib><creatorcontrib>Jarajreh, Duaa A</creatorcontrib><creatorcontrib>Kharabsheh, Ahlam M. Al</creatorcontrib><creatorcontrib>Abufraijeh, Seham M</creatorcontrib><creatorcontrib>Albataineh, Eman M</creatorcontrib><creatorcontrib>Alnawaiseh, Nedal A</creatorcontrib><title>Putative virulence factors of Candida species colonising asymptomatic pregnant Jordanian women</title><title>New Zealand journal of medical laboratory science</title><description>Objectives: Pregnant women are frequently exposed to yeast colonisation and infection compared to non-pregnant women. This study was undertaken to investigate carriage rates of Candida species and their virulence factors in high vaginal samples from asymptomatic pregnant women as a probable predisposing factor for neonatal candidiasis.
Methods: High vaginal swabs were collected from 200 pregnant women from Al-Karak Governmental Hospital, Jordan from March to December 2018. Candida isolates were identified by their growth on CHROMagar Candida and Vitek2 automated system. Some virulence factors were determined.
Results: Among 200 swabs tested, 67 (33.5%) yielded Candida isolates as follows: C. ablicans, 28 (41.8%), while non-albicans Candida (NAC) were 39 (58.2%) isolates (p=0.04). C. parapsilosis was the most prevailed NAC species isolated, 29 (74.4%) followed by C. tropicalis: 6 (15.4%) while C. glabrata and C. krusei, each represented 2 (5.1%). Among C. albicans isolates, 27 (96.4%), 18 (64.3%), and 24 (85.7%) compared to 24 (82.7%), 4 (13.8%), and 25 (86.2%) among C. parapsilosis were positive for proteinase, phospholipase, and haemolysin respectively. The protease activity was the highest detected (88.1% of Candida isolates) compared to other enzymes but did not reach statistical significance between Candida species (p=0.215). Phospholipase activity was significantly detected among C. albicans isolates compared to other species (p <0.001). Insignificant differences in haemolysin production was observed among isolates (p= 0.134).
Conclusions: In Jordanian pregnant women, NAC, mainly C. parapsilosis, were the most frequently isolated Candida species from high vaginal swabs. Routine screening and treatment are recommended for pregnant women, irrespective of symptoms.</description><subject>Asymptomatic</subject><subject>Automation</subject><subject>Candida albicans</subject><subject>Cultures and culture media</subject><subject>Diabetes</subject><subject>Evaluation</subject><subject>Health aspects</subject><subject>Health care</subject><subject>Infection</subject><subject>Infections</subject><subject>Morphology</subject><subject>Mycoses</subject><subject>Phospholipases</subject><subject>Pregnancy</subject><subject>Pregnant women</subject><subject>Vagina</subject><subject>Virulence</subject><subject>Womens health</subject><subject>Yeast fungi</subject><issn>1171-0195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkFtLAzEQhfdBwVr9DwGfK7ls9vJYilcK-lBfDdNksqbsJjVJK_57l1YogszDwJzvnBnmrJgwVrMZZa28KC5T2lBaVkK2k-L9dZchuz2SvYu7Hr1GYkHnEBMJlizAG2eApC1qh4no0AfvkvMdgfQ9bHMYRrcm24idB5_Jc4gGvANPvsKA_qo4t9AnvP7t0-Lt_m61eJwtXx6eFvPlrOMlz7PxFNHUwPi6ssip0aWANa_Q2BpqI6QsqUUmmGzRcgGmKTllzVpWttKwtlZMi5tj7jaGzx2mrDZhF_24UnHZ1KxpORcnqoMelfM25Ah6cEmreVXRtqWiZiN1-w81lsHB6eDRunH-x7A6GuLgshpf1KPOLvi0gZxUQoj645Bz0EPslAlOMaqEYNVJqDnnknFJac2YaMUPfCuJqQ</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Abu-Lubad, Mohammad A</creator><creator>Helaly, Ghada F</creator><creator>Aqel, Amin A</creator><creator>Jarajreh, Duaa A</creator><creator>Kharabsheh, Ahlam M. Al</creator><creator>Abufraijeh, Seham M</creator><creator>Albataineh, Eman M</creator><creator>Alnawaiseh, Nedal A</creator><general>New Zealand Institute of Medical Laboratory Science</general><scope>3V.</scope><scope>7RV</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AYAGU</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>KB0</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20210401</creationdate><title>Putative virulence factors of Candida species colonising asymptomatic pregnant Jordanian women</title><author>Abu-Lubad, Mohammad A ; Helaly, Ghada F ; Aqel, Amin A ; Jarajreh, Duaa A ; Kharabsheh, Ahlam M. Al ; Abufraijeh, Seham M ; Albataineh, Eman M ; Alnawaiseh, Nedal A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g242t-359387a12b6fe20dc43ab26edf7a7d35540fe13159ef23ad842018b56f6cabff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Asymptomatic</topic><topic>Automation</topic><topic>Candida albicans</topic><topic>Cultures and culture media</topic><topic>Diabetes</topic><topic>Evaluation</topic><topic>Health aspects</topic><topic>Health care</topic><topic>Infection</topic><topic>Infections</topic><topic>Morphology</topic><topic>Mycoses</topic><topic>Phospholipases</topic><topic>Pregnancy</topic><topic>Pregnant women</topic><topic>Vagina</topic><topic>Virulence</topic><topic>Womens health</topic><topic>Yeast fungi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abu-Lubad, Mohammad A</creatorcontrib><creatorcontrib>Helaly, Ghada F</creatorcontrib><creatorcontrib>Aqel, Amin A</creatorcontrib><creatorcontrib>Jarajreh, Duaa A</creatorcontrib><creatorcontrib>Kharabsheh, Ahlam M. Al</creatorcontrib><creatorcontrib>Abufraijeh, Seham M</creatorcontrib><creatorcontrib>Albataineh, Eman M</creatorcontrib><creatorcontrib>Alnawaiseh, Nedal A</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Australia & New Zealand Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>New Zealand journal of medical laboratory science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abu-Lubad, Mohammad A</au><au>Helaly, Ghada F</au><au>Aqel, Amin A</au><au>Jarajreh, Duaa A</au><au>Kharabsheh, Ahlam M. Al</au><au>Abufraijeh, Seham M</au><au>Albataineh, Eman M</au><au>Alnawaiseh, Nedal A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Putative virulence factors of Candida species colonising asymptomatic pregnant Jordanian women</atitle><jtitle>New Zealand journal of medical laboratory science</jtitle><date>2021-04-01</date><risdate>2021</risdate><volume>75</volume><issue>1</issue><spage>51</spage><epage>56</epage><pages>51-56</pages><issn>1171-0195</issn><abstract>Objectives: Pregnant women are frequently exposed to yeast colonisation and infection compared to non-pregnant women. This study was undertaken to investigate carriage rates of Candida species and their virulence factors in high vaginal samples from asymptomatic pregnant women as a probable predisposing factor for neonatal candidiasis.
Methods: High vaginal swabs were collected from 200 pregnant women from Al-Karak Governmental Hospital, Jordan from March to December 2018. Candida isolates were identified by their growth on CHROMagar Candida and Vitek2 automated system. Some virulence factors were determined.
Results: Among 200 swabs tested, 67 (33.5%) yielded Candida isolates as follows: C. ablicans, 28 (41.8%), while non-albicans Candida (NAC) were 39 (58.2%) isolates (p=0.04). C. parapsilosis was the most prevailed NAC species isolated, 29 (74.4%) followed by C. tropicalis: 6 (15.4%) while C. glabrata and C. krusei, each represented 2 (5.1%). Among C. albicans isolates, 27 (96.4%), 18 (64.3%), and 24 (85.7%) compared to 24 (82.7%), 4 (13.8%), and 25 (86.2%) among C. parapsilosis were positive for proteinase, phospholipase, and haemolysin respectively. The protease activity was the highest detected (88.1% of Candida isolates) compared to other enzymes but did not reach statistical significance between Candida species (p=0.215). Phospholipase activity was significantly detected among C. albicans isolates compared to other species (p <0.001). Insignificant differences in haemolysin production was observed among isolates (p= 0.134).
Conclusions: In Jordanian pregnant women, NAC, mainly C. parapsilosis, were the most frequently isolated Candida species from high vaginal swabs. Routine screening and treatment are recommended for pregnant women, irrespective of symptoms.</abstract><cop>Auckland</cop><pub>New Zealand Institute of Medical Laboratory Science</pub><tpages>6</tpages></addata></record> |
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| ispartof | New Zealand journal of medical laboratory science, 2021-04, Vol.75 (1), p.51-56 |
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| subjects | Asymptomatic Automation Candida albicans Cultures and culture media Diabetes Evaluation Health aspects Health care Infection Infections Morphology Mycoses Phospholipases Pregnancy Pregnant women Vagina Virulence Womens health Yeast fungi |
| title | Putative virulence factors of Candida species colonising asymptomatic pregnant Jordanian women |
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