Evaluation of antioxidant, antibacterial and cytotoxicity activities of exopolysaccharide from Enterococcus strains isolated from traditional Iranian Kishk
In this study, the antimicrobial effect of exopolysaccharide (EPS) extracted from Enterococcus strains [ E. durans K48 (MT437,248), E. faecium R114 (MT437,249) and E. faecium T52 (MT437,250)] isolated from Kishk was applied against some foodborne pathogenic bacteria using well diffusion and microdil...
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| Veröffentlicht in: | Journal of food measurement & characterization 2021-12, Vol.15 (6), p.5221-5230 |
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| creator | Rahnama Vosough, Paria Habibi Najafi, Mohammad Bagher Edalatian Dovom, Mohammad Reza Javadmanesh, Ali Mayo, Baltasar |
| description | In this study, the antimicrobial effect of exopolysaccharide (EPS) extracted from
Enterococcus
strains [
E. durans
K48 (MT437,248),
E. faecium
R114 (MT437,249) and
E. faecium
T52 (MT437,250)] isolated from Kishk was applied against some foodborne pathogenic bacteria using well diffusion and microdilution methods. The antioxidant activity of EPS was also evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and ferric reducing antioxidant power (FRAP) method. The cytotoxicity effect of EPS on human Gingival Fibroblast (HGF) cell line was also assessed. The results obtained by antimicrobial test showed that the most resistant bacteria to the examined EPS was
Listeria monocytogenes
, and the most susceptible were
Staphylococcus aureus
and
E. faecalis
. The results showed that the DPPH inhibitory percentage of EPS (25 mg/mL) from
E. durans
K48,
E. faecium
R114, and
E. faecium
T52 was 53%, 58% and 64%, respectively. EPS from
E. faecium
T52 displayed the highest reducing power, but statistically, there was no significant difference between the reducing power of EPS T52 and EPS R114 (P ≥ 0.05). The lowest toxicity percentage of EPS k48, EPS T52, and EPS R114 on normal human cell line at a concentration of 0.2 mg/mL was 10%, 15%, and 13%, respectively, which was statistically significant (P |
| doi_str_mv | 10.1007/s11694-021-01092-5 |
| format | Article |
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Enterococcus
strains [
E. durans
K48 (MT437,248),
E. faecium
R114 (MT437,249) and
E. faecium
T52 (MT437,250)] isolated from Kishk was applied against some foodborne pathogenic bacteria using well diffusion and microdilution methods. The antioxidant activity of EPS was also evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and ferric reducing antioxidant power (FRAP) method. The cytotoxicity effect of EPS on human Gingival Fibroblast (HGF) cell line was also assessed. The results obtained by antimicrobial test showed that the most resistant bacteria to the examined EPS was
Listeria monocytogenes
, and the most susceptible were
Staphylococcus aureus
and
E. faecalis
. The results showed that the DPPH inhibitory percentage of EPS (25 mg/mL) from
E. durans
K48,
E. faecium
R114, and
E. faecium
T52 was 53%, 58% and 64%, respectively. EPS from
E. faecium
T52 displayed the highest reducing power, but statistically, there was no significant difference between the reducing power of EPS T52 and EPS R114 (P ≥ 0.05). The lowest toxicity percentage of EPS k48, EPS T52, and EPS R114 on normal human cell line at a concentration of 0.2 mg/mL was 10%, 15%, and 13%, respectively, which was statistically significant (P < 0.05). The obtained results in the present study indicate that EPS from the examined LAB strains with no in vitro cytotoxicity can be a potential source of natural antioxidant and antibacterial agent to be used in food and pharmaceutical industries.</description><identifier>ISSN: 2193-4126</identifier><identifier>EISSN: 2193-4134</identifier><identifier>DOI: 10.1007/s11694-021-01092-5</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Antibacterial agents ; Antioxidants ; Bacteria ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Cytotoxicity ; Engineering ; Enterococcus ; Evaluation ; Exopolysaccharides ; Food industry ; Food Science ; Listeria ; Original Paper ; Pharmaceutical industry ; Scavenging ; Statistical analysis ; Strains (organisms) ; Toxicity</subject><ispartof>Journal of food measurement & characterization, 2021-12, Vol.15 (6), p.5221-5230</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-3e5143056b1554af6c155ab3e7750673f4c49d4c5ff7aa8ef420dc48e6f096ed3</citedby><cites>FETCH-LOGICAL-c319t-3e5143056b1554af6c155ab3e7750673f4c49d4c5ff7aa8ef420dc48e6f096ed3</cites><orcidid>0000-0002-0498-1067</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11694-021-01092-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11694-021-01092-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Rahnama Vosough, Paria</creatorcontrib><creatorcontrib>Habibi Najafi, Mohammad Bagher</creatorcontrib><creatorcontrib>Edalatian Dovom, Mohammad Reza</creatorcontrib><creatorcontrib>Javadmanesh, Ali</creatorcontrib><creatorcontrib>Mayo, Baltasar</creatorcontrib><title>Evaluation of antioxidant, antibacterial and cytotoxicity activities of exopolysaccharide from Enterococcus strains isolated from traditional Iranian Kishk</title><title>Journal of food measurement & characterization</title><addtitle>Food Measure</addtitle><description>In this study, the antimicrobial effect of exopolysaccharide (EPS) extracted from
Enterococcus
strains [
E. durans
K48 (MT437,248),
E. faecium
R114 (MT437,249) and
E. faecium
T52 (MT437,250)] isolated from Kishk was applied against some foodborne pathogenic bacteria using well diffusion and microdilution methods. The antioxidant activity of EPS was also evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and ferric reducing antioxidant power (FRAP) method. The cytotoxicity effect of EPS on human Gingival Fibroblast (HGF) cell line was also assessed. The results obtained by antimicrobial test showed that the most resistant bacteria to the examined EPS was
Listeria monocytogenes
, and the most susceptible were
Staphylococcus aureus
and
E. faecalis
. The results showed that the DPPH inhibitory percentage of EPS (25 mg/mL) from
E. durans
K48,
E. faecium
R114, and
E. faecium
T52 was 53%, 58% and 64%, respectively. EPS from
E. faecium
T52 displayed the highest reducing power, but statistically, there was no significant difference between the reducing power of EPS T52 and EPS R114 (P ≥ 0.05). The lowest toxicity percentage of EPS k48, EPS T52, and EPS R114 on normal human cell line at a concentration of 0.2 mg/mL was 10%, 15%, and 13%, respectively, which was statistically significant (P < 0.05). The obtained results in the present study indicate that EPS from the examined LAB strains with no in vitro cytotoxicity can be a potential source of natural antioxidant and antibacterial agent to be used in food and pharmaceutical industries.</description><subject>Antibacterial agents</subject><subject>Antioxidants</subject><subject>Bacteria</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Cytotoxicity</subject><subject>Engineering</subject><subject>Enterococcus</subject><subject>Evaluation</subject><subject>Exopolysaccharides</subject><subject>Food industry</subject><subject>Food Science</subject><subject>Listeria</subject><subject>Original Paper</subject><subject>Pharmaceutical industry</subject><subject>Scavenging</subject><subject>Statistical analysis</subject><subject>Strains (organisms)</subject><subject>Toxicity</subject><issn>2193-4126</issn><issn>2193-4134</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU1OwzAQhSMEElXpBVhZYkvAjn_SLFFVoAKJDaytqWNTlzQutlM1Z-GyuA2CHas343nfeKSXZZcE3xCMy9tAiKhYjguSY4KrIucn2aggFc0Zoez0ty7EeTYJYY0xJqRkTNBR9jXfQdNBtK5FziBoU7W3ddLrY7MEFbW30KSuRqqPLqa5srFHaWJ3NlodDqTeu61r-gBKrcDbWiPj3QbN24Q75ZTqAgrRg20DssE1EHU9WNJjbQ8HpE8WHloLLXqyYfVxkZ0ZaIKe_Og4e7ufv84e8-eXh8Xs7jlXlFQxp5oTRjEXS8I5AyNUUlhSXZYci5IaplhVM8WNKQGm2rAC14pNtTC4Erqm4-xq2Lv17rPTIcq163w6J8iCTwtWloyI5CoGl_IuBK-N3Hq7Ad9LguUhBznkIFMO8piD5AmiAxSSuX3X_m_1P9Q3Y6yPcQ</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Rahnama Vosough, Paria</creator><creator>Habibi Najafi, Mohammad Bagher</creator><creator>Edalatian Dovom, Mohammad Reza</creator><creator>Javadmanesh, Ali</creator><creator>Mayo, Baltasar</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M0K</scope><scope>M7S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><orcidid>https://orcid.org/0000-0002-0498-1067</orcidid></search><sort><creationdate>20211201</creationdate><title>Evaluation of antioxidant, antibacterial and cytotoxicity activities of exopolysaccharide from Enterococcus strains isolated from traditional Iranian Kishk</title><author>Rahnama Vosough, Paria ; Habibi Najafi, Mohammad Bagher ; Edalatian Dovom, Mohammad Reza ; Javadmanesh, Ali ; Mayo, Baltasar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-3e5143056b1554af6c155ab3e7750673f4c49d4c5ff7aa8ef420dc48e6f096ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibacterial agents</topic><topic>Antioxidants</topic><topic>Bacteria</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Cytotoxicity</topic><topic>Engineering</topic><topic>Enterococcus</topic><topic>Evaluation</topic><topic>Exopolysaccharides</topic><topic>Food industry</topic><topic>Food Science</topic><topic>Listeria</topic><topic>Original Paper</topic><topic>Pharmaceutical industry</topic><topic>Scavenging</topic><topic>Statistical analysis</topic><topic>Strains (organisms)</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahnama Vosough, Paria</creatorcontrib><creatorcontrib>Habibi Najafi, Mohammad Bagher</creatorcontrib><creatorcontrib>Edalatian Dovom, Mohammad Reza</creatorcontrib><creatorcontrib>Javadmanesh, Ali</creatorcontrib><creatorcontrib>Mayo, Baltasar</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Engineering Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><jtitle>Journal of food measurement & characterization</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahnama Vosough, Paria</au><au>Habibi Najafi, Mohammad Bagher</au><au>Edalatian Dovom, Mohammad Reza</au><au>Javadmanesh, Ali</au><au>Mayo, Baltasar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of antioxidant, antibacterial and cytotoxicity activities of exopolysaccharide from Enterococcus strains isolated from traditional Iranian Kishk</atitle><jtitle>Journal of food measurement & characterization</jtitle><stitle>Food Measure</stitle><date>2021-12-01</date><risdate>2021</risdate><volume>15</volume><issue>6</issue><spage>5221</spage><epage>5230</epage><pages>5221-5230</pages><issn>2193-4126</issn><eissn>2193-4134</eissn><abstract>In this study, the antimicrobial effect of exopolysaccharide (EPS) extracted from
Enterococcus
strains [
E. durans
K48 (MT437,248),
E. faecium
R114 (MT437,249) and
E. faecium
T52 (MT437,250)] isolated from Kishk was applied against some foodborne pathogenic bacteria using well diffusion and microdilution methods. The antioxidant activity of EPS was also evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and ferric reducing antioxidant power (FRAP) method. The cytotoxicity effect of EPS on human Gingival Fibroblast (HGF) cell line was also assessed. The results obtained by antimicrobial test showed that the most resistant bacteria to the examined EPS was
Listeria monocytogenes
, and the most susceptible were
Staphylococcus aureus
and
E. faecalis
. The results showed that the DPPH inhibitory percentage of EPS (25 mg/mL) from
E. durans
K48,
E. faecium
R114, and
E. faecium
T52 was 53%, 58% and 64%, respectively. EPS from
E. faecium
T52 displayed the highest reducing power, but statistically, there was no significant difference between the reducing power of EPS T52 and EPS R114 (P ≥ 0.05). The lowest toxicity percentage of EPS k48, EPS T52, and EPS R114 on normal human cell line at a concentration of 0.2 mg/mL was 10%, 15%, and 13%, respectively, which was statistically significant (P < 0.05). The obtained results in the present study indicate that EPS from the examined LAB strains with no in vitro cytotoxicity can be a potential source of natural antioxidant and antibacterial agent to be used in food and pharmaceutical industries.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11694-021-01092-5</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-0498-1067</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2193-4126 |
| ispartof | Journal of food measurement & characterization, 2021-12, Vol.15 (6), p.5221-5230 |
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| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Antibacterial agents Antioxidants Bacteria Chemistry Chemistry and Materials Science Chemistry/Food Science Cytotoxicity Engineering Enterococcus Evaluation Exopolysaccharides Food industry Food Science Listeria Original Paper Pharmaceutical industry Scavenging Statistical analysis Strains (organisms) Toxicity |
| title | Evaluation of antioxidant, antibacterial and cytotoxicity activities of exopolysaccharide from Enterococcus strains isolated from traditional Iranian Kishk |
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