Effect of treatment with resiniferatoxin in an experimental model of pulpal inflammatory in mice
Aim To evaluate whether treatment with resiniferatoxin (RTX) is capable of lowering the plasma levels of PGE2 and TNF‐α, as well as histopathological parameters in inflammation of pulp tissue in a mouse experimental model. Methodology Ten groups of six BALB/c mice were formed as follows: healthy gro...
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| Veröffentlicht in: | International endodontic journal 2021-11, Vol.54 (11), p.2099-2112 |
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| creator | Muñoz‐Carrillo, José Luis Vargas‐Barboza, Jazmín Monserrat Villalobos‐Gutiérrez, Paola Trinidad Flores‐De La Torre, Juan Armando Vazquez‐Alcaraz, Silverio Jafet Gutiérrez‐Coronado, Oscar |
| description | Aim
To evaluate whether treatment with resiniferatoxin (RTX) is capable of lowering the plasma levels of PGE2 and TNF‐α, as well as histopathological parameters in inflammation of pulp tissue in a mouse experimental model.
Methodology
Ten groups of six BALB/c mice were formed as follows: healthy group (HC), healthy group treated with RTX (HRTX), two groups with pulp inflammation at 14 and 18 hours (PI14/PI18), six groups with pulpal inflammation plus treatment with Ibuprofen (IBU14/IBU18), dexamethasone (DEX14/DEX18) and resiniferatoxin (RTX14/RTX18) at 14 and 18 hours, respectively. Pulpal inflammation was induced through occlusal exposure of the pulp of the maxillary first molar. The plasma levels of PGE2 and TNF‐α and the histological parameters of the pulp tissue of the HC and HRTX groups were evaluated at the time of acquiring the animals. In the other groups, the plasma levels of PGE2 and TNF‐α and the histopathological parameters were evaluated at 14 and 18 hours after pulp damage. Plasma levels of PGE2 and TNF‐α were quantified by ELISA, and the histopathological parameters were evaluated by H/E staining. Statistical significance was determined by one‐way analysis of variance (ANOVA) to test for overall differences between group means.
Results
A significant increase (*p |
| doi_str_mv | 10.1111/iej.13606 |
| format | Article |
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To evaluate whether treatment with resiniferatoxin (RTX) is capable of lowering the plasma levels of PGE2 and TNF‐α, as well as histopathological parameters in inflammation of pulp tissue in a mouse experimental model.
Methodology
Ten groups of six BALB/c mice were formed as follows: healthy group (HC), healthy group treated with RTX (HRTX), two groups with pulp inflammation at 14 and 18 hours (PI14/PI18), six groups with pulpal inflammation plus treatment with Ibuprofen (IBU14/IBU18), dexamethasone (DEX14/DEX18) and resiniferatoxin (RTX14/RTX18) at 14 and 18 hours, respectively. Pulpal inflammation was induced through occlusal exposure of the pulp of the maxillary first molar. The plasma levels of PGE2 and TNF‐α and the histological parameters of the pulp tissue of the HC and HRTX groups were evaluated at the time of acquiring the animals. In the other groups, the plasma levels of PGE2 and TNF‐α and the histopathological parameters were evaluated at 14 and 18 hours after pulp damage. Plasma levels of PGE2 and TNF‐α were quantified by ELISA, and the histopathological parameters were evaluated by H/E staining. Statistical significance was determined by one‐way analysis of variance (ANOVA) to test for overall differences between group means.
Results
A significant increase (*p < .05) in plasma levels of PGE2 and TNF‐α occurred 14 and 18 hours after pulp damage. In addition, treatment with RTX significantly decreased (*p < .05) the plasma levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage, as well as the infiltrate of inflammatory cells at 18 hours after pulp damage, similarly to treatment with ibuprofen and dexamethasone.
Conclusion
It was possible to detect systemic levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage. Likewise, treatment with RTX was associated with an anti‐inflammatory effect similar to treatment with ibuprofen and dexamethasone. These findings place resiniferatoxin as a therapeutic alternative in the treatment of inflammatory diseases in Dentistry.
Induction of pulp inflammation and anti‐inflammatory effect of resiniferatoxin in an experimental model of pulpal inflammatory in mice.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.13606</identifier><identifier>PMID: 34375451</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Animals ; Dental Pulp - drug effects ; Dental Pulp - pathology ; Dentistry ; Dexamethasone ; Diterpenes - pharmacology ; Ibuprofen ; Inflammation ; Inflammation - drug therapy ; Inflammatory diseases ; Mice ; Mice, Inbred BALB C ; Models, Theoretical ; Nonsteroidal anti-inflammatory drugs ; Plasma ; Plasma levels ; Prostaglandin E2 ; pulp inflammation ; Resiniferatoxin ; Steroids ; Tumor necrosis factor ; Tumor Necrosis Factor-alpha ; tumour necrosis factor‐α ; Variance analysis</subject><ispartof>International endodontic journal, 2021-11, Vol.54 (11), p.2099-2112</ispartof><rights>2021 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2021 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2021 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3536-d058b411cd77384e39f735d61a4625025be0b3f92fe436d97289ea14d0bbfd833</citedby><cites>FETCH-LOGICAL-c3536-d058b411cd77384e39f735d61a4625025be0b3f92fe436d97289ea14d0bbfd833</cites><orcidid>0000-0003-2394-1455 ; 0000-0001-5689-3619 ; 0000-0002-4660-5641 ; 0000-0002-9403-1566 ; 0000-0001-5043-9407 ; 0000-0003-1819-1143</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.13606$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.13606$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34375451$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muñoz‐Carrillo, José Luis</creatorcontrib><creatorcontrib>Vargas‐Barboza, Jazmín Monserrat</creatorcontrib><creatorcontrib>Villalobos‐Gutiérrez, Paola Trinidad</creatorcontrib><creatorcontrib>Flores‐De La Torre, Juan Armando</creatorcontrib><creatorcontrib>Vazquez‐Alcaraz, Silverio Jafet</creatorcontrib><creatorcontrib>Gutiérrez‐Coronado, Oscar</creatorcontrib><title>Effect of treatment with resiniferatoxin in an experimental model of pulpal inflammatory in mice</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim
To evaluate whether treatment with resiniferatoxin (RTX) is capable of lowering the plasma levels of PGE2 and TNF‐α, as well as histopathological parameters in inflammation of pulp tissue in a mouse experimental model.
Methodology
Ten groups of six BALB/c mice were formed as follows: healthy group (HC), healthy group treated with RTX (HRTX), two groups with pulp inflammation at 14 and 18 hours (PI14/PI18), six groups with pulpal inflammation plus treatment with Ibuprofen (IBU14/IBU18), dexamethasone (DEX14/DEX18) and resiniferatoxin (RTX14/RTX18) at 14 and 18 hours, respectively. Pulpal inflammation was induced through occlusal exposure of the pulp of the maxillary first molar. The plasma levels of PGE2 and TNF‐α and the histological parameters of the pulp tissue of the HC and HRTX groups were evaluated at the time of acquiring the animals. In the other groups, the plasma levels of PGE2 and TNF‐α and the histopathological parameters were evaluated at 14 and 18 hours after pulp damage. Plasma levels of PGE2 and TNF‐α were quantified by ELISA, and the histopathological parameters were evaluated by H/E staining. Statistical significance was determined by one‐way analysis of variance (ANOVA) to test for overall differences between group means.
Results
A significant increase (*p < .05) in plasma levels of PGE2 and TNF‐α occurred 14 and 18 hours after pulp damage. In addition, treatment with RTX significantly decreased (*p < .05) the plasma levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage, as well as the infiltrate of inflammatory cells at 18 hours after pulp damage, similarly to treatment with ibuprofen and dexamethasone.
Conclusion
It was possible to detect systemic levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage. Likewise, treatment with RTX was associated with an anti‐inflammatory effect similar to treatment with ibuprofen and dexamethasone. These findings place resiniferatoxin as a therapeutic alternative in the treatment of inflammatory diseases in Dentistry.
Induction of pulp inflammation and anti‐inflammatory effect of resiniferatoxin in an experimental model of pulpal inflammatory in mice.</description><subject>Animals</subject><subject>Dental Pulp - drug effects</subject><subject>Dental Pulp - pathology</subject><subject>Dentistry</subject><subject>Dexamethasone</subject><subject>Diterpenes - pharmacology</subject><subject>Ibuprofen</subject><subject>Inflammation</subject><subject>Inflammation - drug therapy</subject><subject>Inflammatory diseases</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Models, Theoretical</subject><subject>Nonsteroidal anti-inflammatory drugs</subject><subject>Plasma</subject><subject>Plasma levels</subject><subject>Prostaglandin E2</subject><subject>pulp inflammation</subject><subject>Resiniferatoxin</subject><subject>Steroids</subject><subject>Tumor necrosis factor</subject><subject>Tumor Necrosis Factor-alpha</subject><subject>tumour necrosis factor‐α</subject><subject>Variance analysis</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1Lw0AQhhdRbK0e_AMS8OQh7X5ncxSpWil40fO6SWZxS77cpLT9925M9eYwMAw87zvMi9A1wXMSauFgMydMYnmCpmGKmIqUnKIpJpzFVCkxQRddt8EYC8zIOZowzhLBBZmij6W1kPdRY6Peg-krqPto5_rPyEPnamfBm77ZuzoKbeoI9i14N1CmjKqmgHKQttuyDburbWmqKgj8YeArl8MlOrOm7ODqOGfo_XH59vAcr1-fVg_36zhngsm4wEJlnJC8SBKmOLDUJkwUkhguqcBUZIAzZlNqgTNZpAlVKRjCC5xltlCMzdDt6Nv65msLXa83zdbX4aSmQmGpJBY0UHcjlfum6zxY3YZvjD9ogvWQpQ5Z6p8sA3tzdNxmFRR_5G94AViMwM6VcPjfSa-WL6PlN-9gfbY</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>Muñoz‐Carrillo, José Luis</creator><creator>Vargas‐Barboza, Jazmín Monserrat</creator><creator>Villalobos‐Gutiérrez, Paola Trinidad</creator><creator>Flores‐De La Torre, Juan Armando</creator><creator>Vazquez‐Alcaraz, Silverio Jafet</creator><creator>Gutiérrez‐Coronado, Oscar</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0003-2394-1455</orcidid><orcidid>https://orcid.org/0000-0001-5689-3619</orcidid><orcidid>https://orcid.org/0000-0002-4660-5641</orcidid><orcidid>https://orcid.org/0000-0002-9403-1566</orcidid><orcidid>https://orcid.org/0000-0001-5043-9407</orcidid><orcidid>https://orcid.org/0000-0003-1819-1143</orcidid></search><sort><creationdate>202111</creationdate><title>Effect of treatment with resiniferatoxin in an experimental model of pulpal inflammatory in mice</title><author>Muñoz‐Carrillo, José Luis ; Vargas‐Barboza, Jazmín Monserrat ; Villalobos‐Gutiérrez, Paola Trinidad ; Flores‐De La Torre, Juan Armando ; Vazquez‐Alcaraz, Silverio Jafet ; Gutiérrez‐Coronado, Oscar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3536-d058b411cd77384e39f735d61a4625025be0b3f92fe436d97289ea14d0bbfd833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Dental Pulp - drug effects</topic><topic>Dental Pulp - pathology</topic><topic>Dentistry</topic><topic>Dexamethasone</topic><topic>Diterpenes - pharmacology</topic><topic>Ibuprofen</topic><topic>Inflammation</topic><topic>Inflammation - drug therapy</topic><topic>Inflammatory diseases</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Models, Theoretical</topic><topic>Nonsteroidal anti-inflammatory drugs</topic><topic>Plasma</topic><topic>Plasma levels</topic><topic>Prostaglandin E2</topic><topic>pulp inflammation</topic><topic>Resiniferatoxin</topic><topic>Steroids</topic><topic>Tumor necrosis factor</topic><topic>Tumor Necrosis Factor-alpha</topic><topic>tumour necrosis factor‐α</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muñoz‐Carrillo, José Luis</creatorcontrib><creatorcontrib>Vargas‐Barboza, Jazmín Monserrat</creatorcontrib><creatorcontrib>Villalobos‐Gutiérrez, Paola Trinidad</creatorcontrib><creatorcontrib>Flores‐De La Torre, Juan Armando</creatorcontrib><creatorcontrib>Vazquez‐Alcaraz, Silverio Jafet</creatorcontrib><creatorcontrib>Gutiérrez‐Coronado, Oscar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muñoz‐Carrillo, José Luis</au><au>Vargas‐Barboza, Jazmín Monserrat</au><au>Villalobos‐Gutiérrez, Paola Trinidad</au><au>Flores‐De La Torre, Juan Armando</au><au>Vazquez‐Alcaraz, Silverio Jafet</au><au>Gutiérrez‐Coronado, Oscar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of treatment with resiniferatoxin in an experimental model of pulpal inflammatory in mice</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2021-11</date><risdate>2021</risdate><volume>54</volume><issue>11</issue><spage>2099</spage><epage>2112</epage><pages>2099-2112</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
To evaluate whether treatment with resiniferatoxin (RTX) is capable of lowering the plasma levels of PGE2 and TNF‐α, as well as histopathological parameters in inflammation of pulp tissue in a mouse experimental model.
Methodology
Ten groups of six BALB/c mice were formed as follows: healthy group (HC), healthy group treated with RTX (HRTX), two groups with pulp inflammation at 14 and 18 hours (PI14/PI18), six groups with pulpal inflammation plus treatment with Ibuprofen (IBU14/IBU18), dexamethasone (DEX14/DEX18) and resiniferatoxin (RTX14/RTX18) at 14 and 18 hours, respectively. Pulpal inflammation was induced through occlusal exposure of the pulp of the maxillary first molar. The plasma levels of PGE2 and TNF‐α and the histological parameters of the pulp tissue of the HC and HRTX groups were evaluated at the time of acquiring the animals. In the other groups, the plasma levels of PGE2 and TNF‐α and the histopathological parameters were evaluated at 14 and 18 hours after pulp damage. Plasma levels of PGE2 and TNF‐α were quantified by ELISA, and the histopathological parameters were evaluated by H/E staining. Statistical significance was determined by one‐way analysis of variance (ANOVA) to test for overall differences between group means.
Results
A significant increase (*p < .05) in plasma levels of PGE2 and TNF‐α occurred 14 and 18 hours after pulp damage. In addition, treatment with RTX significantly decreased (*p < .05) the plasma levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage, as well as the infiltrate of inflammatory cells at 18 hours after pulp damage, similarly to treatment with ibuprofen and dexamethasone.
Conclusion
It was possible to detect systemic levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage. Likewise, treatment with RTX was associated with an anti‐inflammatory effect similar to treatment with ibuprofen and dexamethasone. These findings place resiniferatoxin as a therapeutic alternative in the treatment of inflammatory diseases in Dentistry.
Induction of pulp inflammation and anti‐inflammatory effect of resiniferatoxin in an experimental model of pulpal inflammatory in mice.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34375451</pmid><doi>10.1111/iej.13606</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-2394-1455</orcidid><orcidid>https://orcid.org/0000-0001-5689-3619</orcidid><orcidid>https://orcid.org/0000-0002-4660-5641</orcidid><orcidid>https://orcid.org/0000-0002-9403-1566</orcidid><orcidid>https://orcid.org/0000-0001-5043-9407</orcidid><orcidid>https://orcid.org/0000-0003-1819-1143</orcidid></addata></record> |
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| subjects | Animals Dental Pulp - drug effects Dental Pulp - pathology Dentistry Dexamethasone Diterpenes - pharmacology Ibuprofen Inflammation Inflammation - drug therapy Inflammatory diseases Mice Mice, Inbred BALB C Models, Theoretical Nonsteroidal anti-inflammatory drugs Plasma Plasma levels Prostaglandin E2 pulp inflammation Resiniferatoxin Steroids Tumor necrosis factor Tumor Necrosis Factor-alpha tumour necrosis factor‐α Variance analysis |
| title | Effect of treatment with resiniferatoxin in an experimental model of pulpal inflammatory in mice |
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