Isolation and identification of low-density polyethylene degrading novel bacterial strains
Plastics are usually made up of low-density polyethylene (LDPE) that serve as the environmental nuisance. The recalcitrant nature of plastics is a huge concern, whereas the increasing demand has made it difficult to handle the plastic waste that eventually leads to plastic pollution. In recent years...
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| Veröffentlicht in: | Archives of microbiology 2021-11, Vol.203 (9), p.5417-5423 |
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| creator | Nadeem, Habibullah Alia, Khush Bakhat Muneer, Faizan Rasul, Ijaz Siddique, Muhammad Hussnain Azeem, Farrukh Zubair, Muhammad |
| description | Plastics are usually made up of low-density polyethylene (LDPE) that serve as the environmental nuisance. The recalcitrant nature of plastics is a huge concern, whereas the increasing demand has made it difficult to handle the plastic waste that eventually leads to plastic pollution. In recent years, due to increasing demand and high pressure for its safe disposal, plastic biodegradation has gained a lot of attention. In the current study, four bacterial strains were isolated from the solid-waste dumpsites of Faisalabad, Pakistan, using enrichment culture technique. The isolated bacterial strains were capable of growing on media having polystyrene as the sole carbon source. Based on 16S rRNA gene sequencing and phylogenetic analysis of the isolated strains
Serratia
sp.,
Stenotrophomonas
sp. and
Pseudomonas
sp. were identified as the potential strains for the biodegradation of LDPE.
Serratia
sp. resulted in 40% weight loss of the LDPE plastic pieces after 150 days of treatment.
Stenotrophomonas
sp. and
Pseudomonas
species resulted in 32 and 21% weight loss of the treated piece of plastics (LDPE), respectively. Polyethylene pieces were characterized by Fourier-transform infrared spectroscopy (FTIR) analysis before and after biodegradation. The FTIR spectra indicated that the isolated bacterial strains have a good potential to degrade LDPE. Future studies are required to investigate the bacterial genetic makeup, mechanisms of LDPE biodegradation and the factors that can enhance the biodegradable characteristics of these indigenously isolated bacterial strains. |
| doi_str_mv | 10.1007/s00203-021-02521-1 |
| format | Article |
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Serratia
sp.,
Stenotrophomonas
sp. and
Pseudomonas
sp. were identified as the potential strains for the biodegradation of LDPE.
Serratia
sp. resulted in 40% weight loss of the LDPE plastic pieces after 150 days of treatment.
Stenotrophomonas
sp. and
Pseudomonas
species resulted in 32 and 21% weight loss of the treated piece of plastics (LDPE), respectively. Polyethylene pieces were characterized by Fourier-transform infrared spectroscopy (FTIR) analysis before and after biodegradation. The FTIR spectra indicated that the isolated bacterial strains have a good potential to degrade LDPE. Future studies are required to investigate the bacterial genetic makeup, mechanisms of LDPE biodegradation and the factors that can enhance the biodegradable characteristics of these indigenously isolated bacterial strains.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-021-02521-1</identifier><identifier>PMID: 34402947</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Bacteria ; Bacteria - genetics ; Biochemistry ; Biodegradability ; Biodegradation ; Biodegradation, Environmental ; Biomedical and Life Sciences ; Biotechnology ; Carbon sources ; Cell Biology ; Culture techniques ; Density ; Ecology ; Enrichment media ; Fourier transforms ; Gene sequencing ; High pressure ; Infrared analysis ; Infrared spectroscopy ; Life Sciences ; Low density polyethylenes ; Microbial Ecology ; Microbiology ; Original Paper ; Phylogeny ; Plastic debris ; Plastic pollution ; Plastics ; Polyethylene ; Polystyrene ; Polystyrene resins ; Pseudomonas ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Serratia ; Spectrum analysis ; Stenotrophomonas ; Strains (organisms) ; Weight loss</subject><ispartof>Archives of microbiology, 2021-11, Vol.203 (9), p.5417-5423</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-17b4127d9b3e1ec15c527f92135f21611b88d98c86646a0f3c541601404faaec3</citedby><cites>FETCH-LOGICAL-c375t-17b4127d9b3e1ec15c527f92135f21611b88d98c86646a0f3c541601404faaec3</cites><orcidid>0000-0003-4290-5492</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-021-02521-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-021-02521-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34402947$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nadeem, Habibullah</creatorcontrib><creatorcontrib>Alia, Khush Bakhat</creatorcontrib><creatorcontrib>Muneer, Faizan</creatorcontrib><creatorcontrib>Rasul, Ijaz</creatorcontrib><creatorcontrib>Siddique, Muhammad Hussnain</creatorcontrib><creatorcontrib>Azeem, Farrukh</creatorcontrib><creatorcontrib>Zubair, Muhammad</creatorcontrib><title>Isolation and identification of low-density polyethylene degrading novel bacterial strains</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Plastics are usually made up of low-density polyethylene (LDPE) that serve as the environmental nuisance. The recalcitrant nature of plastics is a huge concern, whereas the increasing demand has made it difficult to handle the plastic waste that eventually leads to plastic pollution. In recent years, due to increasing demand and high pressure for its safe disposal, plastic biodegradation has gained a lot of attention. In the current study, four bacterial strains were isolated from the solid-waste dumpsites of Faisalabad, Pakistan, using enrichment culture technique. The isolated bacterial strains were capable of growing on media having polystyrene as the sole carbon source. Based on 16S rRNA gene sequencing and phylogenetic analysis of the isolated strains
Serratia
sp.,
Stenotrophomonas
sp. and
Pseudomonas
sp. were identified as the potential strains for the biodegradation of LDPE.
Serratia
sp. resulted in 40% weight loss of the LDPE plastic pieces after 150 days of treatment.
Stenotrophomonas
sp. and
Pseudomonas
species resulted in 32 and 21% weight loss of the treated piece of plastics (LDPE), respectively. Polyethylene pieces were characterized by Fourier-transform infrared spectroscopy (FTIR) analysis before and after biodegradation. The FTIR spectra indicated that the isolated bacterial strains have a good potential to degrade LDPE. Future studies are required to investigate the bacterial genetic makeup, mechanisms of LDPE biodegradation and the factors that can enhance the biodegradable characteristics of these indigenously isolated bacterial strains.</description><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Biochemistry</subject><subject>Biodegradability</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Carbon sources</subject><subject>Cell Biology</subject><subject>Culture techniques</subject><subject>Density</subject><subject>Ecology</subject><subject>Enrichment media</subject><subject>Fourier transforms</subject><subject>Gene sequencing</subject><subject>High pressure</subject><subject>Infrared analysis</subject><subject>Infrared spectroscopy</subject><subject>Life Sciences</subject><subject>Low density polyethylenes</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Phylogeny</subject><subject>Plastic debris</subject><subject>Plastic pollution</subject><subject>Plastics</subject><subject>Polyethylene</subject><subject>Polystyrene</subject><subject>Polystyrene resins</subject><subject>Pseudomonas</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Serratia</subject><subject>Spectrum analysis</subject><subject>Stenotrophomonas</subject><subject>Strains (organisms)</subject><subject>Weight loss</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kEtLxTAQhYMoen38ARdScF2dSdImXYr4uCC4URA3IU3Ta6Qm16RXuf_eaH3sXMwMnDlzBj5CDhFOEECcJgAKrASKuarccYPMkDNagqAPm2QGDGgpG8Z2yG5KzwBIpZTbZIdxDrThYkYe5ykMenTBF9p3heusH13vzCSFvhjCe5nF5MZ1sQzD2o5P68F6W3R2EXXn_KLw4c0ORavNaKPTQ5HGqJ1P-2Sr10OyB99zj9xfXtydX5c3t1fz87Ob0jBRjSWKliMVXdMyi9ZgZSoq-oYiq3qKNWIrZddII-ua1xp6ZiqONSAH3mttDdsjx1PuMobXlU2jeg6r6PNLRSsJTAhoRHbRyWViSCnaXi2je9FxrRDUJ0414VQZp_rCqTAfHX1Hr9oX2_2e_PDLBjYZUl75hY1_v_-J_QDEh4BC</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Nadeem, Habibullah</creator><creator>Alia, Khush Bakhat</creator><creator>Muneer, Faizan</creator><creator>Rasul, Ijaz</creator><creator>Siddique, Muhammad Hussnain</creator><creator>Azeem, Farrukh</creator><creator>Zubair, Muhammad</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0003-4290-5492</orcidid></search><sort><creationdate>20211101</creationdate><title>Isolation and identification of low-density polyethylene degrading novel bacterial strains</title><author>Nadeem, Habibullah ; Alia, Khush Bakhat ; Muneer, Faizan ; Rasul, Ijaz ; Siddique, Muhammad Hussnain ; Azeem, Farrukh ; Zubair, Muhammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-17b4127d9b3e1ec15c527f92135f21611b88d98c86646a0f3c541601404faaec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bacteria</topic><topic>Bacteria - 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The recalcitrant nature of plastics is a huge concern, whereas the increasing demand has made it difficult to handle the plastic waste that eventually leads to plastic pollution. In recent years, due to increasing demand and high pressure for its safe disposal, plastic biodegradation has gained a lot of attention. In the current study, four bacterial strains were isolated from the solid-waste dumpsites of Faisalabad, Pakistan, using enrichment culture technique. The isolated bacterial strains were capable of growing on media having polystyrene as the sole carbon source. Based on 16S rRNA gene sequencing and phylogenetic analysis of the isolated strains
Serratia
sp.,
Stenotrophomonas
sp. and
Pseudomonas
sp. were identified as the potential strains for the biodegradation of LDPE.
Serratia
sp. resulted in 40% weight loss of the LDPE plastic pieces after 150 days of treatment.
Stenotrophomonas
sp. and
Pseudomonas
species resulted in 32 and 21% weight loss of the treated piece of plastics (LDPE), respectively. Polyethylene pieces were characterized by Fourier-transform infrared spectroscopy (FTIR) analysis before and after biodegradation. The FTIR spectra indicated that the isolated bacterial strains have a good potential to degrade LDPE. Future studies are required to investigate the bacterial genetic makeup, mechanisms of LDPE biodegradation and the factors that can enhance the biodegradable characteristics of these indigenously isolated bacterial strains.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>34402947</pmid><doi>10.1007/s00203-021-02521-1</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-4290-5492</orcidid></addata></record> |
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| ispartof | Archives of microbiology, 2021-11, Vol.203 (9), p.5417-5423 |
| issn | 0302-8933 1432-072X |
| language | eng |
| recordid | cdi_proquest_journals_2580377097 |
| source | MEDLINE; Springer Online Journals Complete |
| subjects | Bacteria Bacteria - genetics Biochemistry Biodegradability Biodegradation Biodegradation, Environmental Biomedical and Life Sciences Biotechnology Carbon sources Cell Biology Culture techniques Density Ecology Enrichment media Fourier transforms Gene sequencing High pressure Infrared analysis Infrared spectroscopy Life Sciences Low density polyethylenes Microbial Ecology Microbiology Original Paper Phylogeny Plastic debris Plastic pollution Plastics Polyethylene Polystyrene Polystyrene resins Pseudomonas RNA, Ribosomal, 16S - genetics rRNA 16S Serratia Spectrum analysis Stenotrophomonas Strains (organisms) Weight loss |
| title | Isolation and identification of low-density polyethylene degrading novel bacterial strains |
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