Fabrication and evaluation of aptamer-conjugated paclitaxel-loaded magnetic nanoparticles for targeted therapy on breast cancer cells
Targeted drug delivery vehicles make it possible to deliver anti-cancer drugs to the cells or tissues of interest. Aptamers are peptide or oligonucleotide molecules that can serve as targeting elements of drug carriers. In the current study, we evaluated the capacity of an aptamer-based drug carrier...
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| Veröffentlicht in: | Molecular biology reports 2021-03, Vol.48 (3), p.2105-2116 |
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| creator | Khodadadi, Emad Mahjoub, Soleiman Arabi, Mehdi Sheikh Najafzadehvarzi, Hossein Nasirian, Vahid |
| description | Targeted drug delivery vehicles make it possible to deliver anti-cancer drugs to the cells or tissues of interest. Aptamers are peptide or oligonucleotide molecules that can serve as targeting elements of drug carriers. In the current study, we evaluated the capacity of an aptamer-based drug carrier to deliver Paclitaxel (PTX) to cancer cells. After being synthesized, SPIONs@PTX-SYL3C aptamer was characterized using different methods, including differential light scattering (DLS), infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Thermal gravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Encapsulation efficiency (EE) and loading efficiency (LE) were also evaluated. The carrier was applied on 4T1, MCF 7, and MCF-10A breast cell lines to evaluate its drug delivery potency and specificity. EE and LE were calculated to be 77.6% and 7.76%, respectively. MTT results revealed that aptameric SPIONs@PTX was more toxic than non-aptameric SPIONs@PTX. Flowcytometry analysis and DAPI staining confirmed that SPIONs@PTX-Aptamer had higher cell internalization rate when compared to non-targeted SPIONs@PTX. Our results indicate that aptamer-conjugated SPIONs@PTX has a good capacity in recognizing its target cells and inhibiting their growth and division. |
| doi_str_mv | 10.1007/s11033-021-06199-y |
| format | Article |
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Aptamers are peptide or oligonucleotide molecules that can serve as targeting elements of drug carriers. In the current study, we evaluated the capacity of an aptamer-based drug carrier to deliver Paclitaxel (PTX) to cancer cells. After being synthesized, SPIONs@PTX-SYL3C aptamer was characterized using different methods, including differential light scattering (DLS), infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Thermal gravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Encapsulation efficiency (EE) and loading efficiency (LE) were also evaluated. The carrier was applied on 4T1, MCF 7, and MCF-10A breast cell lines to evaluate its drug delivery potency and specificity. EE and LE were calculated to be 77.6% and 7.76%, respectively. MTT results revealed that aptameric SPIONs@PTX was more toxic than non-aptameric SPIONs@PTX. Flowcytometry analysis and DAPI staining confirmed that SPIONs@PTX-Aptamer had higher cell internalization rate when compared to non-targeted SPIONs@PTX. Our results indicate that aptamer-conjugated SPIONs@PTX has a good capacity in recognizing its target cells and inhibiting their growth and division.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-021-06199-y</identifier><identifier>PMID: 33635469</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animals ; Antitumor agents ; Aptamers ; Aptamers, Nucleotide - chemistry ; Biomedical and Life Sciences ; Breast cancer ; Breast Neoplasms - drug therapy ; Breast Neoplasms - pathology ; Cancer ; Cell Line, Tumor ; Cell Survival ; Drug delivery ; Drug Liberation ; Endocytosis ; Female ; Fluorescence ; Histology ; Humans ; Infrared spectroscopy ; Inhibitory Concentration 50 ; Internalization ; Life Sciences ; Light scattering ; Magnetite Nanoparticles - chemistry ; Magnetite Nanoparticles - ultrastructure ; Mice ; Molecular Targeted Therapy ; Morphology ; Nanoparticles ; Oligonucleotides ; Original Article ; Paclitaxel ; Paclitaxel - therapeutic use ; Particle Size ; Reproducibility of Results ; Spectroscopy, Fourier Transform Infrared ; Static Electricity ; Thermogravimetry ; Transmission electron microscopy ; X-Ray Diffraction</subject><ispartof>Molecular biology reports, 2021-03, Vol.48 (3), p.2105-2116</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-8f57bd504a3893a7fde7740a7f43b35a5b91ab338bb9e56d2ca9520c572dad903</citedby><cites>FETCH-LOGICAL-c375t-8f57bd504a3893a7fde7740a7f43b35a5b91ab338bb9e56d2ca9520c572dad903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-021-06199-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-021-06199-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33635469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khodadadi, Emad</creatorcontrib><creatorcontrib>Mahjoub, Soleiman</creatorcontrib><creatorcontrib>Arabi, Mehdi Sheikh</creatorcontrib><creatorcontrib>Najafzadehvarzi, Hossein</creatorcontrib><creatorcontrib>Nasirian, Vahid</creatorcontrib><title>Fabrication and evaluation of aptamer-conjugated paclitaxel-loaded magnetic nanoparticles for targeted therapy on breast cancer cells</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Targeted drug delivery vehicles make it possible to deliver anti-cancer drugs to the cells or tissues of interest. Aptamers are peptide or oligonucleotide molecules that can serve as targeting elements of drug carriers. In the current study, we evaluated the capacity of an aptamer-based drug carrier to deliver Paclitaxel (PTX) to cancer cells. After being synthesized, SPIONs@PTX-SYL3C aptamer was characterized using different methods, including differential light scattering (DLS), infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Thermal gravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Encapsulation efficiency (EE) and loading efficiency (LE) were also evaluated. The carrier was applied on 4T1, MCF 7, and MCF-10A breast cell lines to evaluate its drug delivery potency and specificity. EE and LE were calculated to be 77.6% and 7.76%, respectively. MTT results revealed that aptameric SPIONs@PTX was more toxic than non-aptameric SPIONs@PTX. Flowcytometry analysis and DAPI staining confirmed that SPIONs@PTX-Aptamer had higher cell internalization rate when compared to non-targeted SPIONs@PTX. Our results indicate that aptamer-conjugated SPIONs@PTX has a good capacity in recognizing its target cells and inhibiting their growth and division.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animals</subject><subject>Antitumor agents</subject><subject>Aptamers</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>Drug delivery</subject><subject>Drug Liberation</subject><subject>Endocytosis</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Histology</subject><subject>Humans</subject><subject>Infrared spectroscopy</subject><subject>Inhibitory Concentration 50</subject><subject>Internalization</subject><subject>Life Sciences</subject><subject>Light scattering</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>Magnetite Nanoparticles - ultrastructure</subject><subject>Mice</subject><subject>Molecular Targeted Therapy</subject><subject>Morphology</subject><subject>Nanoparticles</subject><subject>Oligonucleotides</subject><subject>Original Article</subject><subject>Paclitaxel</subject><subject>Paclitaxel - therapeutic use</subject><subject>Particle Size</subject><subject>Reproducibility of Results</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Static Electricity</subject><subject>Thermogravimetry</subject><subject>Transmission electron microscopy</subject><subject>X-Ray Diffraction</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kE1vFSEUhonR2NuPP-DCkLhGYRiGYWkaqyZN3NQ1OcCZ69zMHUZgjPcH9H-XOtXuXHGA931O8hDyRvD3gnP9IQvBpWS8EYx3whh2ekF2QmnJWqP7l2THJRes7ZU4I-c5HzjnrdDqNTmTspOq7cyO3N-AS6OHMsaZwhwo_oJp3a5xoLAUOGJiPs6HdQ8FA13AT2OB3zixKUKoL0fYz1hGT2eY4wKpjhNmOsREC6Q9PrbKD0ywnGjFuoSQC_Uwe0zU4zTlS_JqgCnj1dN5Qb7ffLq7_sJuv33-ev3xlnmpVWH9oLQLircgeyNBDwG1bnkdWumkAuWMACdl75xB1YXGg1EN90o3AYLh8oK827hLij9XzMUe4prmutI2SleWbDpTU82W8inmnHCwSxqPkE5WcPto3m7mbTVv_5i3p1p6-4Re3RHDv8pf1TUgt0CuX_Me0_Pu_2AfAFwrkjc</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Khodadadi, Emad</creator><creator>Mahjoub, Soleiman</creator><creator>Arabi, Mehdi Sheikh</creator><creator>Najafzadehvarzi, Hossein</creator><creator>Nasirian, Vahid</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20210301</creationdate><title>Fabrication and evaluation of aptamer-conjugated paclitaxel-loaded magnetic nanoparticles for targeted therapy on breast cancer cells</title><author>Khodadadi, Emad ; Mahjoub, Soleiman ; Arabi, Mehdi Sheikh ; Najafzadehvarzi, Hossein ; Nasirian, Vahid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-8f57bd504a3893a7fde7740a7f43b35a5b91ab338bb9e56d2ca9520c572dad903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animals</topic><topic>Antitumor agents</topic><topic>Aptamers</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - drug therapy</topic><topic>Breast Neoplasms - pathology</topic><topic>Cancer</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival</topic><topic>Drug delivery</topic><topic>Drug Liberation</topic><topic>Endocytosis</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Histology</topic><topic>Humans</topic><topic>Infrared spectroscopy</topic><topic>Inhibitory Concentration 50</topic><topic>Internalization</topic><topic>Life Sciences</topic><topic>Light scattering</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>Magnetite Nanoparticles - ultrastructure</topic><topic>Mice</topic><topic>Molecular Targeted Therapy</topic><topic>Morphology</topic><topic>Nanoparticles</topic><topic>Oligonucleotides</topic><topic>Original Article</topic><topic>Paclitaxel</topic><topic>Paclitaxel - therapeutic use</topic><topic>Particle Size</topic><topic>Reproducibility of Results</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Static Electricity</topic><topic>Thermogravimetry</topic><topic>Transmission electron microscopy</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khodadadi, Emad</creatorcontrib><creatorcontrib>Mahjoub, Soleiman</creatorcontrib><creatorcontrib>Arabi, Mehdi Sheikh</creatorcontrib><creatorcontrib>Najafzadehvarzi, Hossein</creatorcontrib><creatorcontrib>Nasirian, Vahid</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khodadadi, Emad</au><au>Mahjoub, Soleiman</au><au>Arabi, Mehdi Sheikh</au><au>Najafzadehvarzi, Hossein</au><au>Nasirian, Vahid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fabrication and evaluation of aptamer-conjugated paclitaxel-loaded magnetic nanoparticles for targeted therapy on breast cancer cells</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>48</volume><issue>3</issue><spage>2105</spage><epage>2116</epage><pages>2105-2116</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Targeted drug delivery vehicles make it possible to deliver anti-cancer drugs to the cells or tissues of interest. Aptamers are peptide or oligonucleotide molecules that can serve as targeting elements of drug carriers. In the current study, we evaluated the capacity of an aptamer-based drug carrier to deliver Paclitaxel (PTX) to cancer cells. After being synthesized, SPIONs@PTX-SYL3C aptamer was characterized using different methods, including differential light scattering (DLS), infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Thermal gravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Encapsulation efficiency (EE) and loading efficiency (LE) were also evaluated. The carrier was applied on 4T1, MCF 7, and MCF-10A breast cell lines to evaluate its drug delivery potency and specificity. EE and LE were calculated to be 77.6% and 7.76%, respectively. MTT results revealed that aptameric SPIONs@PTX was more toxic than non-aptameric SPIONs@PTX. Flowcytometry analysis and DAPI staining confirmed that SPIONs@PTX-Aptamer had higher cell internalization rate when compared to non-targeted SPIONs@PTX. Our results indicate that aptamer-conjugated SPIONs@PTX has a good capacity in recognizing its target cells and inhibiting their growth and division.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>33635469</pmid><doi>10.1007/s11033-021-06199-y</doi><tpages>12</tpages></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Animals Antitumor agents Aptamers Aptamers, Nucleotide - chemistry Biomedical and Life Sciences Breast cancer Breast Neoplasms - drug therapy Breast Neoplasms - pathology Cancer Cell Line, Tumor Cell Survival Drug delivery Drug Liberation Endocytosis Female Fluorescence Histology Humans Infrared spectroscopy Inhibitory Concentration 50 Internalization Life Sciences Light scattering Magnetite Nanoparticles - chemistry Magnetite Nanoparticles - ultrastructure Mice Molecular Targeted Therapy Morphology Nanoparticles Oligonucleotides Original Article Paclitaxel Paclitaxel - therapeutic use Particle Size Reproducibility of Results Spectroscopy, Fourier Transform Infrared Static Electricity Thermogravimetry Transmission electron microscopy X-Ray Diffraction |
| title | Fabrication and evaluation of aptamer-conjugated paclitaxel-loaded magnetic nanoparticles for targeted therapy on breast cancer cells |
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