PURIFICATION AND CHARACTERIZATION OF AN ENDOGLUCANASE PRODUCED FROM Streptomyces sp. STRAINBPNG23
Streptomyces sp. strain BPNG23 produces five endoglucanases: endo1, endo 2, endo 3, endo 4 and endo 5.The endo2 has been purified and characterized by two subsequent purification steps with ultrafiltration and anion exchange chromatography. The specific activity of the endoglucanase has been found t...
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| Veröffentlicht in: | Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2020-10, Vol.10 (2), p.284-288 |
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| creator | BETTACHE, Azzeddine Estelle, Copinet Azzouz, Zahra Boucherba, Nawel Bouiche, Celia Hamma, Samir Maibeche, Rima Duchiron, Francis Benallaoua, Said |
| description | Streptomyces sp. strain BPNG23 produces five endoglucanases: endo1, endo 2, endo 3, endo 4 and endo 5.The endo2 has been purified and characterized by two subsequent purification steps with ultrafiltration and anion exchange chromatography. The specific activity of the endoglucanase has been found to be 380.65 U/mg. The molecular weight to the endoglucanase 2 has been estimated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealing that this isoenzyme is a 66 KDa monomeric enzyme. It showed an optimum temperature and pH values respectively of 6.0 and 50 °C. It was thermostable, it exhibited a half-life time 6 h with a temperature of 50 °C, the enzyme was activated by several metal ions Mn+2, NH4+, Zn2+, Ca2+, Fe2+, Ni2+ and Co2+. It presents a higher affinity towards carboxymethyl cellulose (CMC) with a Km of 6.37 mg/mL and Vmax of 0.056 μmol/mn. This the first of a study of purification and characterization of an endoglucanase produced by a newly isolated actinobacteria strain in Kabylia region (Algeria). |
| doi_str_mv | 10.15414/jmbfs.2020.10.2.284-288 |
| format | Article |
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STRAINBPNG23</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>BETTACHE, Azzeddine ; Estelle, Copinet ; Azzouz, Zahra ; Boucherba, Nawel ; Bouiche, Celia ; Hamma, Samir ; Maibeche, Rima ; Duchiron, Francis ; Benallaoua, Said</creator><creatorcontrib>BETTACHE, Azzeddine ; Estelle, Copinet ; Azzouz, Zahra ; Boucherba, Nawel ; Bouiche, Celia ; Hamma, Samir ; Maibeche, Rima ; Duchiron, Francis ; Benallaoua, Said</creatorcontrib><description>Streptomyces sp. strain BPNG23 produces five endoglucanases: endo1, endo 2, endo 3, endo 4 and endo 5.The endo2 has been purified and characterized by two subsequent purification steps with ultrafiltration and anion exchange chromatography. The specific activity of the endoglucanase has been found to be 380.65 U/mg. The molecular weight to the endoglucanase 2 has been estimated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealing that this isoenzyme is a 66 KDa monomeric enzyme. It showed an optimum temperature and pH values respectively of 6.0 and 50 °C. It was thermostable, it exhibited a half-life time 6 h with a temperature of 50 °C, the enzyme was activated by several metal ions Mn+2, NH4+, Zn2+, Ca2+, Fe2+, Ni2+ and Co2+. It presents a higher affinity towards carboxymethyl cellulose (CMC) with a Km of 6.37 mg/mL and Vmax of 0.056 μmol/mn. This the first of a study of purification and characterization of an endoglucanase produced by a newly isolated actinobacteria strain in Kabylia region (Algeria).</description><identifier>ISSN: 1338-5178</identifier><identifier>EISSN: 1338-5178</identifier><identifier>DOI: 10.15414/jmbfs.2020.10.2.284-288</identifier><language>eng</language><publisher>Nitra: Faculty of Biotechnology and Food Sciences</publisher><subject>Anion exchange ; Anion exchanging ; Calcium ; Calcium ions ; Carbon dioxide ; Carboxymethyl cellulose ; Carboxymethylcellulose ; Cellulose ; Cobalt ; Electrophoresis ; Endoglucanase ; Enzymes ; Gel electrophoresis ; Iron ; Manganese ; Metal ions ; Molecular weight ; Nickel ; Polyacrylamide ; Purification ; Sodium dodecyl sulfate ; Sodium lauryl sulfate ; Streptomyces ; Ultrafiltration ; Zinc</subject><ispartof>Journal of microbiology, biotechnology and food sciences, 2020-10, Vol.10 (2), p.284-288</ispartof><rights>2020. 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This the first of a study of purification and characterization of an endoglucanase produced by a newly isolated actinobacteria strain in Kabylia region (Algeria).</description><subject>Anion exchange</subject><subject>Anion exchanging</subject><subject>Calcium</subject><subject>Calcium ions</subject><subject>Carbon dioxide</subject><subject>Carboxymethyl cellulose</subject><subject>Carboxymethylcellulose</subject><subject>Cellulose</subject><subject>Cobalt</subject><subject>Electrophoresis</subject><subject>Endoglucanase</subject><subject>Enzymes</subject><subject>Gel electrophoresis</subject><subject>Iron</subject><subject>Manganese</subject><subject>Metal ions</subject><subject>Molecular weight</subject><subject>Nickel</subject><subject>Polyacrylamide</subject><subject>Purification</subject><subject>Sodium dodecyl sulfate</subject><subject>Sodium lauryl sulfate</subject><subject>Streptomyces</subject><subject>Ultrafiltration</subject><subject>Zinc</subject><issn>1338-5178</issn><issn>1338-5178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpNkE1rg0AQhpfSQkOa_7DQs3a_1PVo_UiEVIPRSy_LutmFhqZa1xz677tJeujAMMPDMC88AECMfBwwzF6Op95YnyDiAPKJTzjzCOd3YIEp5V6AI37_b38EK2uPyFUYRyQIFkDuuqYsyjRpy7qCSZXBdJM0SdrmTfl-g3XhOMyrrF5vuzSpkn0Od02ddWmewaKp3-B-nvQ4D6cfpS20ow_3bZOU1euuWhP6BB6M_LR69TeXoCvyNt1423rtcreeojSaPXWIFcWIcs4JJUZGxmhJuQwRUohpRpWMe8kCqvpYG4IPHBtzwDpmpqcqjOgSPN_-jtPwfdZ2FsfhPH25SEGCKIwJizF3V_x2pabB2kkbMU4fJzn9CIzE1am4OhUXpxdGhHPqmtNfUxNlKg</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>BETTACHE, Azzeddine</creator><creator>Estelle, Copinet</creator><creator>Azzouz, Zahra</creator><creator>Boucherba, Nawel</creator><creator>Bouiche, Celia</creator><creator>Hamma, Samir</creator><creator>Maibeche, Rima</creator><creator>Duchiron, Francis</creator><creator>Benallaoua, Said</creator><general>Faculty of Biotechnology and Food Sciences</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20201001</creationdate><title>PURIFICATION AND CHARACTERIZATION OF AN ENDOGLUCANASE PRODUCED FROM Streptomyces sp. 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| subjects | Anion exchange Anion exchanging Calcium Calcium ions Carbon dioxide Carboxymethyl cellulose Carboxymethylcellulose Cellulose Cobalt Electrophoresis Endoglucanase Enzymes Gel electrophoresis Iron Manganese Metal ions Molecular weight Nickel Polyacrylamide Purification Sodium dodecyl sulfate Sodium lauryl sulfate Streptomyces Ultrafiltration Zinc |
| title | PURIFICATION AND CHARACTERIZATION OF AN ENDOGLUCANASE PRODUCED FROM Streptomyces sp. STRAINBPNG23 |
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