PROTEOMICS AT THE SINGLE-CELL LEVEL

PROTEOMICS AT THE SINGLE-CELL LEVEL

According to one study2, the median mammalian protein is present at 18,000 copies per cell. [...]his team could analyse just eight samples per day. According to Zhu, N2 was designed to accommodate another crucial development in single-cell proteomics: multiplexing, which increases throughput. In 201...

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Veröffentlicht in:Nature (London) 2021-09, Vol.597 (7877), p.580-582
1. Verfasser: Perkel, Jeffrey M.
Format: Artikel
Sprache:eng
Schlagworte:
Antibodies
Cell cycle
Cell division
Laboratories
Lysis
Mass spectrometry
Mass spectroscopy
Medical research
Multidisciplinary Sciences
Multiplexing
Peptides
Proteins
Proteomics
Researchers
Science & Technology
Science & Technology - Other Topics
Scientific imaging
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Zusammenfassung:According to one study2, the median mammalian protein is present at 18,000 copies per cell. [...]his team could analyse just eight samples per day. According to Zhu, N2 was designed to accommodate another crucial development in single-cell proteomics: multiplexing, which increases throughput. In 2018, Slavov's team described a method called SCoPE-MS (single-cell proteomics by mass spectrometry)7, which blends a mass-spectrometer-friendly cell-lysis protocol with a protein carrier that increases the amount of material available for sequencing.
ISSN:0028-0836
1476-4687
DOI:10.1038/d41586-021-02530-6