Finding Species-Specific Extracellular Surface-Facing Proteomes in Toxic Dinoflagellates
As a sequel to our previous report of the existence of species-specific protein/peptide expression profiles (PEPs) acquired by mass spectrometry in some dinoflagellates, we established, with the help of a plasma-membrane-impermeable labeling agent, a surface amphiesmal protein extraction method (SAP...
Gespeichert in:
| Veröffentlicht in: | Toxins 2021-09, Vol.13 (9), p.624 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 9 |
| container_start_page | 624 |
| container_title | Toxins |
| container_volume | 13 |
| creator | Chan, Kenrick Kai-Yuen Kong, Hang-Kin Tse, Sirius Pui-Kam Chan, Zoe Lo, Pak-Yeung Kwok, Kevin W H Lo, Samuel Chun-Lap |
| description | As a sequel to our previous report of the existence of species-specific protein/peptide expression profiles (PEPs) acquired by mass spectrometry in some dinoflagellates, we established, with the help of a plasma-membrane-impermeable labeling agent, a surface amphiesmal protein extraction method (SAPE) to label and capture species-specific surface proteins (SSSPs) as well as saxitoxins-producing-species-specific surface proteins (Stx-SSPs) that face the extracellular space (i.e., SSSPs
and Stx-SSPs
). Five selected toxic dinoflagellates,
,
and
were used in this study. Transcriptomic databases of these five species were also constructed. With the aid of liquid chromatography linked-tandem mass spectrometry (LC-MS/MS) and the transcriptomic databases of these species, extracellularly facing membrane proteomes of the five different species were identified. Within these proteomes, 16 extracellular-facing and functionally significant transport proteins were found. Furthermore, 10 SSSPs and 6 Stx-SSPs were identified as amphiesmal proteins but not facing outward to the extracellular environment. We also found SSSPs
and Stx-SSPs
in the proteomes. The potential functional correlation of these proteins towards the production of saxitoxins in dinoflagellates and the degree of species specificity were discussed accordingly. |
| doi_str_mv | 10.3390/toxins13090624 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_proquest_journals_2576503206</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_b1131a6d8e624104bd942590b201388b</doaj_id><sourcerecordid>2576503206</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-d15a796c85ad39dd05837b309f84ffadaba187f77eb495f3a28c6968cb1814b53</originalsourceid><addsrcrecordid>eNpVUV1LHDEUDWJRsb72UQb6PDbfHy-CqNsKQgta6FvI55pldrJNZor99826Vty83OTec0_OvQeATwheEKLglyk_p7EiAhXkmB6AEwwF7jln6PDd_Ric1bqC7RCCFBJH4JhQxinH6gT8WqTRp3HZPWyCS6H2LzEm190-T8W4MAzzYEr3MJfYXv3CuC36R8lTyOtQuzR2j02G627SmONglq3DTKF-BB-iGWo4e42n4Ofi9vH6W3___evd9dV97yhRU-8RM0JxJ5nxRHkPmSTCtomipDEab6xBUkQhgqWKRWKwdFxx6SySiFpGTsHdjtdns9Kbktam_NXZJP2SyGWpTZmSG4K2CBFkuJehbQtBar2imCloMUREStu4Lndcm9mug3dhbCsY9kj3K2N60sv8R0sqCEVbMZ9fCUr-PYc66VWey9jm15gJziDBkDfUxQ7lSq61hPj2A4J6a6zeN7Y1nL_X9Qb_byP5B8FxoBg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2576503206</pqid></control><display><type>article</type><title>Finding Species-Specific Extracellular Surface-Facing Proteomes in Toxic Dinoflagellates</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Chan, Kenrick Kai-Yuen ; Kong, Hang-Kin ; Tse, Sirius Pui-Kam ; Chan, Zoe ; Lo, Pak-Yeung ; Kwok, Kevin W H ; Lo, Samuel Chun-Lap</creator><creatorcontrib>Chan, Kenrick Kai-Yuen ; Kong, Hang-Kin ; Tse, Sirius Pui-Kam ; Chan, Zoe ; Lo, Pak-Yeung ; Kwok, Kevin W H ; Lo, Samuel Chun-Lap</creatorcontrib><description>As a sequel to our previous report of the existence of species-specific protein/peptide expression profiles (PEPs) acquired by mass spectrometry in some dinoflagellates, we established, with the help of a plasma-membrane-impermeable labeling agent, a surface amphiesmal protein extraction method (SAPE) to label and capture species-specific surface proteins (SSSPs) as well as saxitoxins-producing-species-specific surface proteins (Stx-SSPs) that face the extracellular space (i.e., SSSPs
and Stx-SSPs
). Five selected toxic dinoflagellates,
,
and
were used in this study. Transcriptomic databases of these five species were also constructed. With the aid of liquid chromatography linked-tandem mass spectrometry (LC-MS/MS) and the transcriptomic databases of these species, extracellularly facing membrane proteomes of the five different species were identified. Within these proteomes, 16 extracellular-facing and functionally significant transport proteins were found. Furthermore, 10 SSSPs and 6 Stx-SSPs were identified as amphiesmal proteins but not facing outward to the extracellular environment. We also found SSSPs
and Stx-SSPs
in the proteomes. The potential functional correlation of these proteins towards the production of saxitoxins in dinoflagellates and the degree of species specificity were discussed accordingly.</description><identifier>ISSN: 2072-6651</identifier><identifier>EISSN: 2072-6651</identifier><identifier>DOI: 10.3390/toxins13090624</identifier><identifier>PMID: 34564629</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Algal Proteins - chemistry ; cell surface labeling ; Chromatography, Liquid ; Cyanobacteria ; Dinoflagellata ; Dinoflagellates ; Dinoflagellida - chemistry ; Genomes ; Labeling ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Membranes ; Microorganisms ; Morphology ; orthogroup inference ; Peptides ; Poisoning ; Protein transport ; Proteins ; Proteome - chemistry ; Proteomes ; proteomics ; Protozoan Proteins - chemistry ; Reagents ; Shellfish ; Species ; Species Specificity ; Specific surface ; Spectroscopy ; Tandem Mass Spectrometry ; toxic dinoflagellates ; Water quality</subject><ispartof>Toxins, 2021-09, Vol.13 (9), p.624</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c439t-d15a796c85ad39dd05837b309f84ffadaba187f77eb495f3a28c6968cb1814b53</cites><orcidid>0000-0001-6204-5392</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473415/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473415/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34564629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chan, Kenrick Kai-Yuen</creatorcontrib><creatorcontrib>Kong, Hang-Kin</creatorcontrib><creatorcontrib>Tse, Sirius Pui-Kam</creatorcontrib><creatorcontrib>Chan, Zoe</creatorcontrib><creatorcontrib>Lo, Pak-Yeung</creatorcontrib><creatorcontrib>Kwok, Kevin W H</creatorcontrib><creatorcontrib>Lo, Samuel Chun-Lap</creatorcontrib><title>Finding Species-Specific Extracellular Surface-Facing Proteomes in Toxic Dinoflagellates</title><title>Toxins</title><addtitle>Toxins (Basel)</addtitle><description>As a sequel to our previous report of the existence of species-specific protein/peptide expression profiles (PEPs) acquired by mass spectrometry in some dinoflagellates, we established, with the help of a plasma-membrane-impermeable labeling agent, a surface amphiesmal protein extraction method (SAPE) to label and capture species-specific surface proteins (SSSPs) as well as saxitoxins-producing-species-specific surface proteins (Stx-SSPs) that face the extracellular space (i.e., SSSPs
and Stx-SSPs
). Five selected toxic dinoflagellates,
,
and
were used in this study. Transcriptomic databases of these five species were also constructed. With the aid of liquid chromatography linked-tandem mass spectrometry (LC-MS/MS) and the transcriptomic databases of these species, extracellularly facing membrane proteomes of the five different species were identified. Within these proteomes, 16 extracellular-facing and functionally significant transport proteins were found. Furthermore, 10 SSSPs and 6 Stx-SSPs were identified as amphiesmal proteins but not facing outward to the extracellular environment. We also found SSSPs
and Stx-SSPs
in the proteomes. The potential functional correlation of these proteins towards the production of saxitoxins in dinoflagellates and the degree of species specificity were discussed accordingly.</description><subject>Algal Proteins - chemistry</subject><subject>cell surface labeling</subject><subject>Chromatography, Liquid</subject><subject>Cyanobacteria</subject><subject>Dinoflagellata</subject><subject>Dinoflagellates</subject><subject>Dinoflagellida - chemistry</subject><subject>Genomes</subject><subject>Labeling</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Membranes</subject><subject>Microorganisms</subject><subject>Morphology</subject><subject>orthogroup inference</subject><subject>Peptides</subject><subject>Poisoning</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Proteome - chemistry</subject><subject>Proteomes</subject><subject>proteomics</subject><subject>Protozoan Proteins - chemistry</subject><subject>Reagents</subject><subject>Shellfish</subject><subject>Species</subject><subject>Species Specificity</subject><subject>Specific surface</subject><subject>Spectroscopy</subject><subject>Tandem Mass Spectrometry</subject><subject>toxic dinoflagellates</subject><subject>Water quality</subject><issn>2072-6651</issn><issn>2072-6651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNpVUV1LHDEUDWJRsb72UQb6PDbfHy-CqNsKQgta6FvI55pldrJNZor99826Vty83OTec0_OvQeATwheEKLglyk_p7EiAhXkmB6AEwwF7jln6PDd_Ric1bqC7RCCFBJH4JhQxinH6gT8WqTRp3HZPWyCS6H2LzEm190-T8W4MAzzYEr3MJfYXv3CuC36R8lTyOtQuzR2j02G627SmONglq3DTKF-BB-iGWo4e42n4Ofi9vH6W3___evd9dV97yhRU-8RM0JxJ5nxRHkPmSTCtomipDEab6xBUkQhgqWKRWKwdFxx6SySiFpGTsHdjtdns9Kbktam_NXZJP2SyGWpTZmSG4K2CBFkuJehbQtBar2imCloMUREStu4Lndcm9mug3dhbCsY9kj3K2N60sv8R0sqCEVbMZ9fCUr-PYc66VWey9jm15gJziDBkDfUxQ7lSq61hPj2A4J6a6zeN7Y1nL_X9Qb_byP5B8FxoBg</recordid><startdate>20210906</startdate><enddate>20210906</enddate><creator>Chan, Kenrick Kai-Yuen</creator><creator>Kong, Hang-Kin</creator><creator>Tse, Sirius Pui-Kam</creator><creator>Chan, Zoe</creator><creator>Lo, Pak-Yeung</creator><creator>Kwok, Kevin W H</creator><creator>Lo, Samuel Chun-Lap</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T7</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-6204-5392</orcidid></search><sort><creationdate>20210906</creationdate><title>Finding Species-Specific Extracellular Surface-Facing Proteomes in Toxic Dinoflagellates</title><author>Chan, Kenrick Kai-Yuen ; Kong, Hang-Kin ; Tse, Sirius Pui-Kam ; Chan, Zoe ; Lo, Pak-Yeung ; Kwok, Kevin W H ; Lo, Samuel Chun-Lap</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-d15a796c85ad39dd05837b309f84ffadaba187f77eb495f3a28c6968cb1814b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Algal Proteins - chemistry</topic><topic>cell surface labeling</topic><topic>Chromatography, Liquid</topic><topic>Cyanobacteria</topic><topic>Dinoflagellata</topic><topic>Dinoflagellates</topic><topic>Dinoflagellida - chemistry</topic><topic>Genomes</topic><topic>Labeling</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Membranes</topic><topic>Microorganisms</topic><topic>Morphology</topic><topic>orthogroup inference</topic><topic>Peptides</topic><topic>Poisoning</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>Proteome - chemistry</topic><topic>Proteomes</topic><topic>proteomics</topic><topic>Protozoan Proteins - chemistry</topic><topic>Reagents</topic><topic>Shellfish</topic><topic>Species</topic><topic>Species Specificity</topic><topic>Specific surface</topic><topic>Spectroscopy</topic><topic>Tandem Mass Spectrometry</topic><topic>toxic dinoflagellates</topic><topic>Water quality</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chan, Kenrick Kai-Yuen</creatorcontrib><creatorcontrib>Kong, Hang-Kin</creatorcontrib><creatorcontrib>Tse, Sirius Pui-Kam</creatorcontrib><creatorcontrib>Chan, Zoe</creatorcontrib><creatorcontrib>Lo, Pak-Yeung</creatorcontrib><creatorcontrib>Kwok, Kevin W H</creatorcontrib><creatorcontrib>Lo, Samuel Chun-Lap</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Toxins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chan, Kenrick Kai-Yuen</au><au>Kong, Hang-Kin</au><au>Tse, Sirius Pui-Kam</au><au>Chan, Zoe</au><au>Lo, Pak-Yeung</au><au>Kwok, Kevin W H</au><au>Lo, Samuel Chun-Lap</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Finding Species-Specific Extracellular Surface-Facing Proteomes in Toxic Dinoflagellates</atitle><jtitle>Toxins</jtitle><addtitle>Toxins (Basel)</addtitle><date>2021-09-06</date><risdate>2021</risdate><volume>13</volume><issue>9</issue><spage>624</spage><pages>624-</pages><issn>2072-6651</issn><eissn>2072-6651</eissn><abstract>As a sequel to our previous report of the existence of species-specific protein/peptide expression profiles (PEPs) acquired by mass spectrometry in some dinoflagellates, we established, with the help of a plasma-membrane-impermeable labeling agent, a surface amphiesmal protein extraction method (SAPE) to label and capture species-specific surface proteins (SSSPs) as well as saxitoxins-producing-species-specific surface proteins (Stx-SSPs) that face the extracellular space (i.e., SSSPs
and Stx-SSPs
). Five selected toxic dinoflagellates,
,
and
were used in this study. Transcriptomic databases of these five species were also constructed. With the aid of liquid chromatography linked-tandem mass spectrometry (LC-MS/MS) and the transcriptomic databases of these species, extracellularly facing membrane proteomes of the five different species were identified. Within these proteomes, 16 extracellular-facing and functionally significant transport proteins were found. Furthermore, 10 SSSPs and 6 Stx-SSPs were identified as amphiesmal proteins but not facing outward to the extracellular environment. We also found SSSPs
and Stx-SSPs
in the proteomes. The potential functional correlation of these proteins towards the production of saxitoxins in dinoflagellates and the degree of species specificity were discussed accordingly.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>34564629</pmid><doi>10.3390/toxins13090624</doi><orcidid>https://orcid.org/0000-0001-6204-5392</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2072-6651 |
| ispartof | Toxins, 2021-09, Vol.13 (9), p.624 |
| issn | 2072-6651 2072-6651 |
| language | eng |
| recordid | cdi_proquest_journals_2576503206 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Algal Proteins - chemistry cell surface labeling Chromatography, Liquid Cyanobacteria Dinoflagellata Dinoflagellates Dinoflagellida - chemistry Genomes Labeling Liquid chromatography Mass spectrometry Mass spectroscopy Membranes Microorganisms Morphology orthogroup inference Peptides Poisoning Protein transport Proteins Proteome - chemistry Proteomes proteomics Protozoan Proteins - chemistry Reagents Shellfish Species Species Specificity Specific surface Spectroscopy Tandem Mass Spectrometry toxic dinoflagellates Water quality |
| title | Finding Species-Specific Extracellular Surface-Facing Proteomes in Toxic Dinoflagellates |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T20%3A18%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Finding%20Species-Specific%20Extracellular%20Surface-Facing%20Proteomes%20in%20Toxic%20Dinoflagellates&rft.jtitle=Toxins&rft.au=Chan,%20Kenrick%20Kai-Yuen&rft.date=2021-09-06&rft.volume=13&rft.issue=9&rft.spage=624&rft.pages=624-&rft.issn=2072-6651&rft.eissn=2072-6651&rft_id=info:doi/10.3390/toxins13090624&rft_dat=%3Cproquest_doaj_%3E2576503206%3C/proquest_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2576503206&rft_id=info:pmid/34564629&rft_doaj_id=oai_doaj_org_article_b1131a6d8e624104bd942590b201388b&rfr_iscdi=true |