Eicosanoid‐induced calcium signaling mediates cellular immune responses of Tenebrio molitor
Calcium signaling is associated with actin cytoskeleton rearrangement. It is required for expressing cellular immune responses of insect hemocytes. Eicosanoids mediate immune responses by cytoskeleton rearrangement against various pathogen infections in insects. The objective of this study was to in...
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| Veröffentlicht in: | Entomologia experimentalis et applicata 2021-10, Vol.169 (10), p.888-898 |
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| description | Calcium signaling is associated with actin cytoskeleton rearrangement. It is required for expressing cellular immune responses of insect hemocytes. Eicosanoids mediate immune responses by cytoskeleton rearrangement against various pathogen infections in insects. The objective of this study was to investigate cellular immune responses of a mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae). The hypothesis tested was that immune responses would be mediated by calcium signaling associated with eicosanoid mediation. Larvae of T. molitor possessed at least four morphological types of hemocytes, most (almost 75%) of which were granulocytes and plasmatocytes. They all exhibited a hemocyte‐spreading behavior by F‐actin extension. Upon bacterial infection, hemocytes exhibited phagocytosis and formed hemocytic nodules. Intracellular calcium was detected in hemocytes. Its amount increased with increasing incubation time after bacterial infection. Inhibition of calcium influx or release from endoplasmic reticulum using specific inhibitors prevented cellular immune responses. Interestingly, treatment with an eicosanoid biosynthesis inhibitor suppressed calcium signals induced by bacterial challenge. Furthermore, treatment with naproxen, a prostaglandin (PG) synthesis inhibitor, or esculetin, a leukotriene synthesis inhibitor, also inhibited the increase of calcium concentration in hemocytes. Among eicosanoids, PGE2 highly stimulated the increase of calcium signals. These results suggest that cellular immune responses of T. molitor are triggered by calcium signaling, which is up‐regulated by eicosanoids.
Calcium signaling may be involved in cellular immune responses in insects. However, functional association of the two has not been well documented. Cellular immune responses were demonstrated in Tenebrio molitor (Coleoptera: Tenebrionidae). Calcium ions (Ca2+) are co‐localized with F‐actin in spread hemocytes upon immune challenge. Prostaglandin E2 stimulates calcium signals in hemocytes of T. molitor. These results suggest that cellular immune responses of T. molitor are triggered by calcium signaling, which is up‐regulated by eicosanoids. |
| doi_str_mv | 10.1111/eea.13037 |
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Calcium signaling may be involved in cellular immune responses in insects. However, functional association of the two has not been well documented. Cellular immune responses were demonstrated in Tenebrio molitor (Coleoptera: Tenebrionidae). Calcium ions (Ca2+) are co‐localized with F‐actin in spread hemocytes upon immune challenge. Prostaglandin E2 stimulates calcium signals in hemocytes of T. molitor. These results suggest that cellular immune responses of T. molitor are triggered by calcium signaling, which is up‐regulated by eicosanoids.</description><identifier>ISSN: 0013-8703</identifier><identifier>EISSN: 1570-7458</identifier><identifier>DOI: 10.1111/eea.13037</identifier><language>eng</language><publisher>Amsterdam: Wiley Subscription Services, Inc</publisher><subject>Actin ; Bacteria ; Bacterial diseases ; Bacterial infections ; Biosynthesis ; Calcium ; Calcium (intracellular) ; Calcium influx ; Calcium signalling ; Coleoptera ; cytoskeleon rearrangement ; Cytoskeleton ; Eicosanoids ; Endoplasmic reticulum ; granulocyte ; hemocyte ; Hemocytes ; Immune response ; Immune response (cell-mediated) ; immunity ; Infections ; Insects ; Larvae ; Leukocytes (granulocytic) ; mealworm ; Naproxen ; Nodules ; pathogen infection ; Phagocytosis ; plasmatocyte ; Plasmatocytes ; Prostaglandin E2 ; Signaling ; Tenebrio molitor ; Tenebrionidae</subject><ispartof>Entomologia experimentalis et applicata, 2021-10, Vol.169 (10), p.888-898</ispartof><rights>2021 The Netherlands Entomological Society</rights><rights>Entomologia Experimentalis et Applicata © 2021 The Netherlands Entomological Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2977-d591bd3c0848142124a12468962b406affad77777b8e903107f683e2215d19643</citedby><cites>FETCH-LOGICAL-c2977-d591bd3c0848142124a12468962b406affad77777b8e903107f683e2215d19643</cites><orcidid>0000-0002-6840-2167</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Feea.13037$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Feea.13037$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Roy, Miltan Chandra</creatorcontrib><creatorcontrib>Kim, Yonggyun</creatorcontrib><title>Eicosanoid‐induced calcium signaling mediates cellular immune responses of Tenebrio molitor</title><title>Entomologia experimentalis et applicata</title><description>Calcium signaling is associated with actin cytoskeleton rearrangement. It is required for expressing cellular immune responses of insect hemocytes. Eicosanoids mediate immune responses by cytoskeleton rearrangement against various pathogen infections in insects. The objective of this study was to investigate cellular immune responses of a mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae). The hypothesis tested was that immune responses would be mediated by calcium signaling associated with eicosanoid mediation. Larvae of T. molitor possessed at least four morphological types of hemocytes, most (almost 75%) of which were granulocytes and plasmatocytes. They all exhibited a hemocyte‐spreading behavior by F‐actin extension. Upon bacterial infection, hemocytes exhibited phagocytosis and formed hemocytic nodules. Intracellular calcium was detected in hemocytes. Its amount increased with increasing incubation time after bacterial infection. Inhibition of calcium influx or release from endoplasmic reticulum using specific inhibitors prevented cellular immune responses. Interestingly, treatment with an eicosanoid biosynthesis inhibitor suppressed calcium signals induced by bacterial challenge. Furthermore, treatment with naproxen, a prostaglandin (PG) synthesis inhibitor, or esculetin, a leukotriene synthesis inhibitor, also inhibited the increase of calcium concentration in hemocytes. Among eicosanoids, PGE2 highly stimulated the increase of calcium signals. These results suggest that cellular immune responses of T. molitor are triggered by calcium signaling, which is up‐regulated by eicosanoids.
Calcium signaling may be involved in cellular immune responses in insects. However, functional association of the two has not been well documented. Cellular immune responses were demonstrated in Tenebrio molitor (Coleoptera: Tenebrionidae). Calcium ions (Ca2+) are co‐localized with F‐actin in spread hemocytes upon immune challenge. Prostaglandin E2 stimulates calcium signals in hemocytes of T. molitor. These results suggest that cellular immune responses of T. molitor are triggered by calcium signaling, which is up‐regulated by eicosanoids.</description><subject>Actin</subject><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>Bacterial infections</subject><subject>Biosynthesis</subject><subject>Calcium</subject><subject>Calcium (intracellular)</subject><subject>Calcium influx</subject><subject>Calcium signalling</subject><subject>Coleoptera</subject><subject>cytoskeleon rearrangement</subject><subject>Cytoskeleton</subject><subject>Eicosanoids</subject><subject>Endoplasmic reticulum</subject><subject>granulocyte</subject><subject>hemocyte</subject><subject>Hemocytes</subject><subject>Immune response</subject><subject>Immune response (cell-mediated)</subject><subject>immunity</subject><subject>Infections</subject><subject>Insects</subject><subject>Larvae</subject><subject>Leukocytes (granulocytic)</subject><subject>mealworm</subject><subject>Naproxen</subject><subject>Nodules</subject><subject>pathogen infection</subject><subject>Phagocytosis</subject><subject>plasmatocyte</subject><subject>Plasmatocytes</subject><subject>Prostaglandin E2</subject><subject>Signaling</subject><subject>Tenebrio molitor</subject><subject>Tenebrionidae</subject><issn>0013-8703</issn><issn>1570-7458</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kM1KAzEQx4MoWKsH3yDgycO2-djd7B5LWT-g4KUeJWST2ZKS3dSki_TmI_iMPomp9eofhjnMb4bhh9AtJTOaMgdQM8oJF2doQgtBMpEX1TmaEEJ5VgnCL9FVjFtCiBA1naC3xmof1eCt-f78soMZNRisldN27HG0m0E5O2xwD8aqPUSswbnRqYBt348D4ABx54eYJr7DaxigDdbj3ju79-EaXXTKRbj561P0-tCsl0_Z6uXxeblYZZrVQmSmqGlruCZVXtGcUZarVGVVl6zNSam6ThlxTFtBTTgloisrDozRwtC6zPkU3Z3u7oJ_HyHu5daPIb0eJSuSgboSjCTq_kTp4GMM0MldsL0KB0mJPNqTyZ78tZfY-Yn9sA4O_4OyaRanjR8Ns3E5</recordid><startdate>202110</startdate><enddate>202110</enddate><creator>Roy, Miltan Chandra</creator><creator>Kim, Yonggyun</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-6840-2167</orcidid></search><sort><creationdate>202110</creationdate><title>Eicosanoid‐induced calcium signaling mediates cellular immune responses of Tenebrio molitor</title><author>Roy, Miltan Chandra ; Kim, Yonggyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2977-d591bd3c0848142124a12468962b406affad77777b8e903107f683e2215d19643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Actin</topic><topic>Bacteria</topic><topic>Bacterial diseases</topic><topic>Bacterial infections</topic><topic>Biosynthesis</topic><topic>Calcium</topic><topic>Calcium (intracellular)</topic><topic>Calcium influx</topic><topic>Calcium signalling</topic><topic>Coleoptera</topic><topic>cytoskeleon rearrangement</topic><topic>Cytoskeleton</topic><topic>Eicosanoids</topic><topic>Endoplasmic reticulum</topic><topic>granulocyte</topic><topic>hemocyte</topic><topic>Hemocytes</topic><topic>Immune response</topic><topic>Immune response (cell-mediated)</topic><topic>immunity</topic><topic>Infections</topic><topic>Insects</topic><topic>Larvae</topic><topic>Leukocytes (granulocytic)</topic><topic>mealworm</topic><topic>Naproxen</topic><topic>Nodules</topic><topic>pathogen infection</topic><topic>Phagocytosis</topic><topic>plasmatocyte</topic><topic>Plasmatocytes</topic><topic>Prostaglandin E2</topic><topic>Signaling</topic><topic>Tenebrio molitor</topic><topic>Tenebrionidae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roy, Miltan Chandra</creatorcontrib><creatorcontrib>Kim, Yonggyun</creatorcontrib><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Entomologia experimentalis et applicata</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roy, Miltan Chandra</au><au>Kim, Yonggyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Eicosanoid‐induced calcium signaling mediates cellular immune responses of Tenebrio molitor</atitle><jtitle>Entomologia experimentalis et applicata</jtitle><date>2021-10</date><risdate>2021</risdate><volume>169</volume><issue>10</issue><spage>888</spage><epage>898</epage><pages>888-898</pages><issn>0013-8703</issn><eissn>1570-7458</eissn><abstract>Calcium signaling is associated with actin cytoskeleton rearrangement. It is required for expressing cellular immune responses of insect hemocytes. Eicosanoids mediate immune responses by cytoskeleton rearrangement against various pathogen infections in insects. The objective of this study was to investigate cellular immune responses of a mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae). The hypothesis tested was that immune responses would be mediated by calcium signaling associated with eicosanoid mediation. Larvae of T. molitor possessed at least four morphological types of hemocytes, most (almost 75%) of which were granulocytes and plasmatocytes. They all exhibited a hemocyte‐spreading behavior by F‐actin extension. Upon bacterial infection, hemocytes exhibited phagocytosis and formed hemocytic nodules. Intracellular calcium was detected in hemocytes. Its amount increased with increasing incubation time after bacterial infection. Inhibition of calcium influx or release from endoplasmic reticulum using specific inhibitors prevented cellular immune responses. Interestingly, treatment with an eicosanoid biosynthesis inhibitor suppressed calcium signals induced by bacterial challenge. Furthermore, treatment with naproxen, a prostaglandin (PG) synthesis inhibitor, or esculetin, a leukotriene synthesis inhibitor, also inhibited the increase of calcium concentration in hemocytes. Among eicosanoids, PGE2 highly stimulated the increase of calcium signals. These results suggest that cellular immune responses of T. molitor are triggered by calcium signaling, which is up‐regulated by eicosanoids.
Calcium signaling may be involved in cellular immune responses in insects. However, functional association of the two has not been well documented. Cellular immune responses were demonstrated in Tenebrio molitor (Coleoptera: Tenebrionidae). Calcium ions (Ca2+) are co‐localized with F‐actin in spread hemocytes upon immune challenge. Prostaglandin E2 stimulates calcium signals in hemocytes of T. molitor. These results suggest that cellular immune responses of T. molitor are triggered by calcium signaling, which is up‐regulated by eicosanoids.</abstract><cop>Amsterdam</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/eea.13037</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6840-2167</orcidid></addata></record> |
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| subjects | Actin Bacteria Bacterial diseases Bacterial infections Biosynthesis Calcium Calcium (intracellular) Calcium influx Calcium signalling Coleoptera cytoskeleon rearrangement Cytoskeleton Eicosanoids Endoplasmic reticulum granulocyte hemocyte Hemocytes Immune response Immune response (cell-mediated) immunity Infections Insects Larvae Leukocytes (granulocytic) mealworm Naproxen Nodules pathogen infection Phagocytosis plasmatocyte Plasmatocytes Prostaglandin E2 Signaling Tenebrio molitor Tenebrionidae |
| title | Eicosanoid‐induced calcium signaling mediates cellular immune responses of Tenebrio molitor |
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