In Silico: γ-Oryzanol as Anti-inflammatory During Folliculogenesis in Rattus Novergicus Exposed to Pyrethroid Aerosol

The use of insect repellent is a disease preventive effort due to mosquito vectors as well as sources of pollutants that disrupt human health, especially reproductive health. This study aimed to determine the effect of γ-Oryzanol on the expression of Foxo3a, TNF-α, GDF-9 in the folliculogenesis of mice (Rattus Novergicus) exposed to pyrethroid aerosol mosquitoes. The research use bioinformatics technique with in silico approach. The result of study showing that γ-oryzanol has the potential as an anti-inflammatory and NO scavenger, the influence of the active compound γ-oryzanol on Foxo3 expression obtained pathway analysis with the results of the prediction of the target HITPICK able to bind to APP, AKT1 and Mtor expressions through modification of post-translational Foxo1, Effect of γ-oryzanol on Gdf9 expression, through bonding with APP, AKT1, AKT3, Pik3cg and modifying Smad 4 post-translation, The effect of the active compound γ-oryzanol on TNF-α expression via APP binds with AKT1, AKT3, AKT2 then activates IkbkB and bindings with Casp3, Fadd, Tradd, Tnfrsf1a, Tnfrsf1b, Traf3, Birc2, Ikbkg. Based on average part length analysis AKT1 is the fastest interact with protein target. The conclusion, γ-oryzanol has predicted to be used for the treatment of insecticide exposure, because it can act as an anti-inflamatory, and lipid perosidase inhibitor. This condition influences normally folliculogenesis with stable expression of Foxo3a, GDF 9 and TNF-α.