Biosurfactant-producing and anthracene-degrading bacteria from oil contaminated soil
Anthracene, together with other polycyclic aromatic hydrocarbons (PAHs), is a persistent and toxic soil contaminant because its low water solubility and high hydrophobicity. The effectiveness of PAH degradation could be enhanced by using biosurfactant. In this study, we isolated bacteria that were a...
Gespeichert in:
| Veröffentlicht in: | Journal of physics. Conference series 2019-12, Vol.1402 (3), p.33049 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | 33049 |
| container_title | Journal of physics. Conference series |
| container_volume | 1402 |
| creator | Kurniati, T H Rusmana, I Suryani, A Mubarik, N R |
| description | Anthracene, together with other polycyclic aromatic hydrocarbons (PAHs), is a persistent and toxic soil contaminant because its low water solubility and high hydrophobicity. The effectiveness of PAH degradation could be enhanced by using biosurfactant. In this study, we isolated bacteria that were able to degrade anthracene and also produce biosurfactant. Samples were taken from oil contaminated soil at Jakarta Bay. Isolation was done in a Minimal Salt Medium (MSM) supplemented with 1% crude oil. Screening methods for biosurfactant production include Blood Hemolysis, Drop Collapse Assay and Oil Displacement Assay. Three isolates which have biosurfactant production and anthracene degradation capability was identified based on 16SrRNA gene. These isolates were identified as Ochrobactrum intermedium AMA9, Ochrobactrum tritici CHA60, and Pseudomonas stutzeri CRA7. Based on GC-MS analysis, Ochrobactrum intermedium AMA9 showed high ability to degrade 84,79% anthracene in liquid MSM medium supplemented by 100 ppm anthracene after seven days incubation. |
| doi_str_mv | 10.1088/1742-6596/1402/3/033049 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2568300340</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2568300340</sourcerecordid><originalsourceid>FETCH-LOGICAL-c413t-69bc76158e40b1b9950efbaa84aa4c714c4e4c3589fc323f834530b7e9d042f53</originalsourceid><addsrcrecordid>eNqFkEtLxDAQgIMouK7-BgvehNqkk7TpURefLCi4nkOax9plt6lJe_Dfm1JZEQQPYULmm5nMh9A5wVcEc56RkuZpwaoiIxTnGWQYANPqAM32mcP9nfNjdBLCBkcIoJyh1U3jwuCtVL1s-7TzTg-qadeJbHU8_buXyrQm1WbtpR4TdUSNb2RivdslrtkmyrW93DWt7I1OQnw5RUdWboM5-45z9HZ3u1o8pMvn-8fF9TJVlECfFlWtyoIwbiiuSV1VDBtbS8mplFSVhCpqqALGK6sgB8uBMsB1aSqNaW4ZzNHF1Dd--2MwoRcbN_g2jhQ5KzjELSmOVDlRyrsQvLGi881O-k9BsBgVilGOGEWJUaEAMSmMlZdTZeO6n9ZPL4vX36DotI0w_AH_N-ILLwGBLw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2568300340</pqid></control><display><type>article</type><title>Biosurfactant-producing and anthracene-degrading bacteria from oil contaminated soil</title><source>Institute of Physics Open Access Journal Titles</source><source>Institute of Physics IOPscience extra</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>EZB Electronic Journals Library</source><creator>Kurniati, T H ; Rusmana, I ; Suryani, A ; Mubarik, N R</creator><creatorcontrib>Kurniati, T H ; Rusmana, I ; Suryani, A ; Mubarik, N R</creatorcontrib><description>Anthracene, together with other polycyclic aromatic hydrocarbons (PAHs), is a persistent and toxic soil contaminant because its low water solubility and high hydrophobicity. The effectiveness of PAH degradation could be enhanced by using biosurfactant. In this study, we isolated bacteria that were able to degrade anthracene and also produce biosurfactant. Samples were taken from oil contaminated soil at Jakarta Bay. Isolation was done in a Minimal Salt Medium (MSM) supplemented with 1% crude oil. Screening methods for biosurfactant production include Blood Hemolysis, Drop Collapse Assay and Oil Displacement Assay. Three isolates which have biosurfactant production and anthracene degradation capability was identified based on 16SrRNA gene. These isolates were identified as Ochrobactrum intermedium AMA9, Ochrobactrum tritici CHA60, and Pseudomonas stutzeri CRA7. Based on GC-MS analysis, Ochrobactrum intermedium AMA9 showed high ability to degrade 84,79% anthracene in liquid MSM medium supplemented by 100 ppm anthracene after seven days incubation.</description><identifier>ISSN: 1742-6588</identifier><identifier>EISSN: 1742-6596</identifier><identifier>DOI: 10.1088/1742-6596/1402/3/033049</identifier><language>eng</language><publisher>Bristol: IOP Publishing</publisher><subject>Anthracene ; Contaminants ; Degradation ; Hydrophobicity ; Physics ; Polycyclic aromatic hydrocarbons ; Production methods ; Soil bacteria ; Soil contamination ; Soils ; Surfactants</subject><ispartof>Journal of physics. Conference series, 2019-12, Vol.1402 (3), p.33049</ispartof><rights>Published under licence by IOP Publishing Ltd</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-69bc76158e40b1b9950efbaa84aa4c714c4e4c3589fc323f834530b7e9d042f53</citedby><cites>FETCH-LOGICAL-c413t-69bc76158e40b1b9950efbaa84aa4c714c4e4c3589fc323f834530b7e9d042f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/1742-6596/1402/3/033049/pdf$$EPDF$$P50$$Giop$$Hfree_for_read</linktopdf><link.rule.ids>314,780,784,27923,27924,38867,38889,53839,53866</link.rule.ids></links><search><creatorcontrib>Kurniati, T H</creatorcontrib><creatorcontrib>Rusmana, I</creatorcontrib><creatorcontrib>Suryani, A</creatorcontrib><creatorcontrib>Mubarik, N R</creatorcontrib><title>Biosurfactant-producing and anthracene-degrading bacteria from oil contaminated soil</title><title>Journal of physics. Conference series</title><addtitle>J. Phys.: Conf. Ser</addtitle><description>Anthracene, together with other polycyclic aromatic hydrocarbons (PAHs), is a persistent and toxic soil contaminant because its low water solubility and high hydrophobicity. The effectiveness of PAH degradation could be enhanced by using biosurfactant. In this study, we isolated bacteria that were able to degrade anthracene and also produce biosurfactant. Samples were taken from oil contaminated soil at Jakarta Bay. Isolation was done in a Minimal Salt Medium (MSM) supplemented with 1% crude oil. Screening methods for biosurfactant production include Blood Hemolysis, Drop Collapse Assay and Oil Displacement Assay. Three isolates which have biosurfactant production and anthracene degradation capability was identified based on 16SrRNA gene. These isolates were identified as Ochrobactrum intermedium AMA9, Ochrobactrum tritici CHA60, and Pseudomonas stutzeri CRA7. Based on GC-MS analysis, Ochrobactrum intermedium AMA9 showed high ability to degrade 84,79% anthracene in liquid MSM medium supplemented by 100 ppm anthracene after seven days incubation.</description><subject>Anthracene</subject><subject>Contaminants</subject><subject>Degradation</subject><subject>Hydrophobicity</subject><subject>Physics</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>Production methods</subject><subject>Soil bacteria</subject><subject>Soil contamination</subject><subject>Soils</subject><subject>Surfactants</subject><issn>1742-6588</issn><issn>1742-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>O3W</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqFkEtLxDAQgIMouK7-BgvehNqkk7TpURefLCi4nkOax9plt6lJe_Dfm1JZEQQPYULmm5nMh9A5wVcEc56RkuZpwaoiIxTnGWQYANPqAM32mcP9nfNjdBLCBkcIoJyh1U3jwuCtVL1s-7TzTg-qadeJbHU8_buXyrQm1WbtpR4TdUSNb2RivdslrtkmyrW93DWt7I1OQnw5RUdWboM5-45z9HZ3u1o8pMvn-8fF9TJVlECfFlWtyoIwbiiuSV1VDBtbS8mplFSVhCpqqALGK6sgB8uBMsB1aSqNaW4ZzNHF1Dd--2MwoRcbN_g2jhQ5KzjELSmOVDlRyrsQvLGi881O-k9BsBgVilGOGEWJUaEAMSmMlZdTZeO6n9ZPL4vX36DotI0w_AH_N-ILLwGBLw</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Kurniati, T H</creator><creator>Rusmana, I</creator><creator>Suryani, A</creator><creator>Mubarik, N R</creator><general>IOP Publishing</general><scope>O3W</scope><scope>TSCCA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>L7M</scope><scope>P5Z</scope><scope>P62</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20191201</creationdate><title>Biosurfactant-producing and anthracene-degrading bacteria from oil contaminated soil</title><author>Kurniati, T H ; Rusmana, I ; Suryani, A ; Mubarik, N R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-69bc76158e40b1b9950efbaa84aa4c714c4e4c3589fc323f834530b7e9d042f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anthracene</topic><topic>Contaminants</topic><topic>Degradation</topic><topic>Hydrophobicity</topic><topic>Physics</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>Production methods</topic><topic>Soil bacteria</topic><topic>Soil contamination</topic><topic>Soils</topic><topic>Surfactants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kurniati, T H</creatorcontrib><creatorcontrib>Rusmana, I</creatorcontrib><creatorcontrib>Suryani, A</creatorcontrib><creatorcontrib>Mubarik, N R</creatorcontrib><collection>Institute of Physics Open Access Journal Titles</collection><collection>IOPscience (Open Access)</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Aerospace Database</collection><collection>SciTech Premium Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of physics. Conference series</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kurniati, T H</au><au>Rusmana, I</au><au>Suryani, A</au><au>Mubarik, N R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosurfactant-producing and anthracene-degrading bacteria from oil contaminated soil</atitle><jtitle>Journal of physics. Conference series</jtitle><addtitle>J. Phys.: Conf. Ser</addtitle><date>2019-12-01</date><risdate>2019</risdate><volume>1402</volume><issue>3</issue><spage>33049</spage><pages>33049-</pages><issn>1742-6588</issn><eissn>1742-6596</eissn><abstract>Anthracene, together with other polycyclic aromatic hydrocarbons (PAHs), is a persistent and toxic soil contaminant because its low water solubility and high hydrophobicity. The effectiveness of PAH degradation could be enhanced by using biosurfactant. In this study, we isolated bacteria that were able to degrade anthracene and also produce biosurfactant. Samples were taken from oil contaminated soil at Jakarta Bay. Isolation was done in a Minimal Salt Medium (MSM) supplemented with 1% crude oil. Screening methods for biosurfactant production include Blood Hemolysis, Drop Collapse Assay and Oil Displacement Assay. Three isolates which have biosurfactant production and anthracene degradation capability was identified based on 16SrRNA gene. These isolates were identified as Ochrobactrum intermedium AMA9, Ochrobactrum tritici CHA60, and Pseudomonas stutzeri CRA7. Based on GC-MS analysis, Ochrobactrum intermedium AMA9 showed high ability to degrade 84,79% anthracene in liquid MSM medium supplemented by 100 ppm anthracene after seven days incubation.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><doi>10.1088/1742-6596/1402/3/033049</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1742-6588 |
| ispartof | Journal of physics. Conference series, 2019-12, Vol.1402 (3), p.33049 |
| issn | 1742-6588 1742-6596 |
| language | eng |
| recordid | cdi_proquest_journals_2568300340 |
| source | Institute of Physics Open Access Journal Titles; Institute of Physics IOPscience extra; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Anthracene Contaminants Degradation Hydrophobicity Physics Polycyclic aromatic hydrocarbons Production methods Soil bacteria Soil contamination Soils Surfactants |
| title | Biosurfactant-producing and anthracene-degrading bacteria from oil contaminated soil |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T17%3A35%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biosurfactant-producing%20and%20anthracene-degrading%20bacteria%20from%20oil%20contaminated%20soil&rft.jtitle=Journal%20of%20physics.%20Conference%20series&rft.au=Kurniati,%20T%20H&rft.date=2019-12-01&rft.volume=1402&rft.issue=3&rft.spage=33049&rft.pages=33049-&rft.issn=1742-6588&rft.eissn=1742-6596&rft_id=info:doi/10.1088/1742-6596/1402/3/033049&rft_dat=%3Cproquest_cross%3E2568300340%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2568300340&rft_id=info:pmid/&rfr_iscdi=true |