Influence of soil microbes on Escherichia coli O157:H7 survival in soil rinse and artificial soil
Aims This research investigated the influence of soil microbiota on Escherichia coli O157:H7 survival in soil rinse and artificial soil. Additionally, the influence of selected soil bacteria on E. coli O157:H7 in soil environments was determined. Methods and Results Escherichia coli O157:H7 counts (...
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| Veröffentlicht in: | Journal of applied microbiology 2021-09, Vol.131 (3), p.1531-1538 |
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| creator | Baker, C.A. De, J. Schneider, K.R. |
| description | Aims
This research investigated the influence of soil microbiota on Escherichia coli O157:H7 survival in soil rinse and artificial soil. Additionally, the influence of selected soil bacteria on E. coli O157:H7 in soil environments was determined.
Methods and Results
Escherichia coli O157:H7 counts (log CFU per ml or g−1) were determined by spread plating: (i) artificial soil amended with soil rinse (filter‐sterilized and unfiltered) at 30°C; (ii) unfiltered soil rinse (50 ml) treated with cycloheximide (200 μg ml−1), vancomycin (40 μg ml−1), heat (80°C, 15 min) and no treatment (control) for 7 days at 30°C and (iii) filtered soil rinse with selected soil bacterial isolates over 7 days. There was a significant difference (P = 0·027) in E. coli O157:H7 counts after 35 days between artificial soils amended with filtered (4·45 ± 0·29) and non‐filtered (1·83 ± 0·33) soil rinse. There were significant differences (P |
| doi_str_mv | 10.1111/jam.15039 |
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| fullrecord | <record><control><sourceid>proquest_webof</sourceid><recordid>TN_cdi_proquest_journals_2568277161</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2568277161</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3539-8f4caa474c1752bf20655cfd91926d73c610e8a2c3d649701024d838014da643</originalsourceid><addsrcrecordid>eNqNkE1LHTEUhkNpqdZ20T9QAt20yGi-M-NOLtYPFDfuQ24mwXOZSWwyY_HfN9e5dSEIzSYHzvOec3gQ-krJEa3veGPHIyoJ796hfcqVbJjS7P1zLRpJNNtDn0rZEEI5keoj2uNctpzSbh_ZyxiG2UfncQq4JBjwCC6ntS84RXxW3L3P4O7BYpcGwLdU6pMLjcucH-HRDhjiksoQi8c29tjmCQI4qM1t5zP6EOxQ_Jfdf4Dufp3drS6a69vzy9XpdeO45F3TBuGsFVo4qiVbB0aUlC70He2Y6jV3ihLfWuZ4r0SnCSVM9C1vCRW9VYIfoB_L2Iecfs--TGaE4vww2OjTXAwTbSc7SZWs6PdX6CbNOdbjDJOqZVpTRSv1c6GqjVKyD-Yhw2jzk6HEbLWbqt08a6_st93EeT36_oX857kChwvwx69TKA62xl8wQohignAhakW2q9v_p1cw2QlSXKU5TjV6vIvC4J_ePtlcnd4st_8FPvypyA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2568277161</pqid></control><display><type>article</type><title>Influence of soil microbes on Escherichia coli O157:H7 survival in soil rinse and artificial soil</title><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Baker, C.A. ; De, J. ; Schneider, K.R.</creator><creatorcontrib>Baker, C.A. ; De, J. ; Schneider, K.R.</creatorcontrib><description>Aims
This research investigated the influence of soil microbiota on Escherichia coli O157:H7 survival in soil rinse and artificial soil. Additionally, the influence of selected soil bacteria on E. coli O157:H7 in soil environments was determined.
Methods and Results
Escherichia coli O157:H7 counts (log CFU per ml or g−1) were determined by spread plating: (i) artificial soil amended with soil rinse (filter‐sterilized and unfiltered) at 30°C; (ii) unfiltered soil rinse (50 ml) treated with cycloheximide (200 μg ml−1), vancomycin (40 μg ml−1), heat (80°C, 15 min) and no treatment (control) for 7 days at 30°C and (iii) filtered soil rinse with selected soil bacterial isolates over 7 days. There was a significant difference (P = 0·027) in E. coli O157:H7 counts after 35 days between artificial soils amended with filtered (4·45 ± 0·29) and non‐filtered (1·83 ± 0·33) soil rinse. There were significant differences (P < 0·05) in E. coli O157:H7 counts after 3 days of incubation between soil rinse treatments (heat (7·04 ± 0·03), cycloheximide (6·94 ± 0·05), vancomycin (4·26 ± 0·98) and control (5·00 ± 0·93)). Lastly, a significant difference (P < 0·05) in E. coli O157:H7 counts was observed after 3 days of incubation at 30°C in filtered soil rinse when incubated with Paenibacillus alvei versus other soil bacterial isolates evaluated.
Conclusions
Soil microbiota isolated from Florida sandy soil influenced E. coli O157:H7 survival. Specifically, P. alvei reduced E. coli O157:H7 by over 3 log CFU per ml after 3 days of incubation at 30°C in filtered soil rinse.
Significance and Impact of the Study
This research identified soil bacterial isolates that may reduce E. coli O157:H7 in the soil environment and be used in future biocontrol applications.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.15039</identifier><identifier>PMID: 33583119</identifier><language>eng</language><publisher>OXFORD: Oxford Univ Press</publisher><subject>artificial soil ; Bacteria ; Biological control ; Biotechnology & Applied Microbiology ; Cycloheximide ; E coli ; E. coli O157:H7 ; Escherichia coli ; Life Sciences & Biomedicine ; Microbiology ; Microbiota ; Paenibacillus ; Sandy soils ; Science & Technology ; Soil amendment ; Soil bacteria ; Soil environment ; Soil investigations ; Soil microorganisms ; soil rinse ; Survival ; Vancomycin</subject><ispartof>Journal of applied microbiology, 2021-09, Vol.131 (3), p.1531-1538</ispartof><rights>2021 The Society for Applied Microbiology</rights><rights>2021 The Society for Applied Microbiology.</rights><rights>Copyright © 2021 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>2</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000624034400001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c3539-8f4caa474c1752bf20655cfd91926d73c610e8a2c3d649701024d838014da643</citedby><cites>FETCH-LOGICAL-c3539-8f4caa474c1752bf20655cfd91926d73c610e8a2c3d649701024d838014da643</cites><orcidid>0000-0001-9799-9401 ; 0000-0003-0145-3418</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.15039$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.15039$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27928,27929,45578,45579</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33583119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baker, C.A.</creatorcontrib><creatorcontrib>De, J.</creatorcontrib><creatorcontrib>Schneider, K.R.</creatorcontrib><title>Influence of soil microbes on Escherichia coli O157:H7 survival in soil rinse and artificial soil</title><title>Journal of applied microbiology</title><addtitle>J APPL MICROBIOL</addtitle><addtitle>J Appl Microbiol</addtitle><description>Aims
This research investigated the influence of soil microbiota on Escherichia coli O157:H7 survival in soil rinse and artificial soil. Additionally, the influence of selected soil bacteria on E. coli O157:H7 in soil environments was determined.
Methods and Results
Escherichia coli O157:H7 counts (log CFU per ml or g−1) were determined by spread plating: (i) artificial soil amended with soil rinse (filter‐sterilized and unfiltered) at 30°C; (ii) unfiltered soil rinse (50 ml) treated with cycloheximide (200 μg ml−1), vancomycin (40 μg ml−1), heat (80°C, 15 min) and no treatment (control) for 7 days at 30°C and (iii) filtered soil rinse with selected soil bacterial isolates over 7 days. There was a significant difference (P = 0·027) in E. coli O157:H7 counts after 35 days between artificial soils amended with filtered (4·45 ± 0·29) and non‐filtered (1·83 ± 0·33) soil rinse. There were significant differences (P < 0·05) in E. coli O157:H7 counts after 3 days of incubation between soil rinse treatments (heat (7·04 ± 0·03), cycloheximide (6·94 ± 0·05), vancomycin (4·26 ± 0·98) and control (5·00 ± 0·93)). Lastly, a significant difference (P < 0·05) in E. coli O157:H7 counts was observed after 3 days of incubation at 30°C in filtered soil rinse when incubated with Paenibacillus alvei versus other soil bacterial isolates evaluated.
Conclusions
Soil microbiota isolated from Florida sandy soil influenced E. coli O157:H7 survival. Specifically, P. alvei reduced E. coli O157:H7 by over 3 log CFU per ml after 3 days of incubation at 30°C in filtered soil rinse.
Significance and Impact of the Study
This research identified soil bacterial isolates that may reduce E. coli O157:H7 in the soil environment and be used in future biocontrol applications.</description><subject>artificial soil</subject><subject>Bacteria</subject><subject>Biological control</subject><subject>Biotechnology & Applied Microbiology</subject><subject>Cycloheximide</subject><subject>E coli</subject><subject>E. coli O157:H7</subject><subject>Escherichia coli</subject><subject>Life Sciences & Biomedicine</subject><subject>Microbiology</subject><subject>Microbiota</subject><subject>Paenibacillus</subject><subject>Sandy soils</subject><subject>Science & Technology</subject><subject>Soil amendment</subject><subject>Soil bacteria</subject><subject>Soil environment</subject><subject>Soil investigations</subject><subject>Soil microorganisms</subject><subject>soil rinse</subject><subject>Survival</subject><subject>Vancomycin</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><recordid>eNqNkE1LHTEUhkNpqdZ20T9QAt20yGi-M-NOLtYPFDfuQ24mwXOZSWwyY_HfN9e5dSEIzSYHzvOec3gQ-krJEa3veGPHIyoJ796hfcqVbJjS7P1zLRpJNNtDn0rZEEI5keoj2uNctpzSbh_ZyxiG2UfncQq4JBjwCC6ntS84RXxW3L3P4O7BYpcGwLdU6pMLjcucH-HRDhjiksoQi8c29tjmCQI4qM1t5zP6EOxQ_Jfdf4Dufp3drS6a69vzy9XpdeO45F3TBuGsFVo4qiVbB0aUlC70He2Y6jV3ihLfWuZ4r0SnCSVM9C1vCRW9VYIfoB_L2Iecfs--TGaE4vww2OjTXAwTbSc7SZWs6PdX6CbNOdbjDJOqZVpTRSv1c6GqjVKyD-Yhw2jzk6HEbLWbqt08a6_st93EeT36_oX857kChwvwx69TKA62xl8wQohignAhakW2q9v_p1cw2QlSXKU5TjV6vIvC4J_ePtlcnd4st_8FPvypyA</recordid><startdate>202109</startdate><enddate>202109</enddate><creator>Baker, C.A.</creator><creator>De, J.</creator><creator>Schneider, K.R.</creator><general>Oxford Univ Press</general><general>Oxford University Press</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9799-9401</orcidid><orcidid>https://orcid.org/0000-0003-0145-3418</orcidid></search><sort><creationdate>202109</creationdate><title>Influence of soil microbes on Escherichia coli O157:H7 survival in soil rinse and artificial soil</title><author>Baker, C.A. ; De, J. ; Schneider, K.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-8f4caa474c1752bf20655cfd91926d73c610e8a2c3d649701024d838014da643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>artificial soil</topic><topic>Bacteria</topic><topic>Biological control</topic><topic>Biotechnology & Applied Microbiology</topic><topic>Cycloheximide</topic><topic>E coli</topic><topic>E. coli O157:H7</topic><topic>Escherichia coli</topic><topic>Life Sciences & Biomedicine</topic><topic>Microbiology</topic><topic>Microbiota</topic><topic>Paenibacillus</topic><topic>Sandy soils</topic><topic>Science & Technology</topic><topic>Soil amendment</topic><topic>Soil bacteria</topic><topic>Soil environment</topic><topic>Soil investigations</topic><topic>Soil microorganisms</topic><topic>soil rinse</topic><topic>Survival</topic><topic>Vancomycin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baker, C.A.</creatorcontrib><creatorcontrib>De, J.</creatorcontrib><creatorcontrib>Schneider, K.R.</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baker, C.A.</au><au>De, J.</au><au>Schneider, K.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of soil microbes on Escherichia coli O157:H7 survival in soil rinse and artificial soil</atitle><jtitle>Journal of applied microbiology</jtitle><stitle>J APPL MICROBIOL</stitle><addtitle>J Appl Microbiol</addtitle><date>2021-09</date><risdate>2021</risdate><volume>131</volume><issue>3</issue><spage>1531</spage><epage>1538</epage><pages>1531-1538</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
This research investigated the influence of soil microbiota on Escherichia coli O157:H7 survival in soil rinse and artificial soil. Additionally, the influence of selected soil bacteria on E. coli O157:H7 in soil environments was determined.
Methods and Results
Escherichia coli O157:H7 counts (log CFU per ml or g−1) were determined by spread plating: (i) artificial soil amended with soil rinse (filter‐sterilized and unfiltered) at 30°C; (ii) unfiltered soil rinse (50 ml) treated with cycloheximide (200 μg ml−1), vancomycin (40 μg ml−1), heat (80°C, 15 min) and no treatment (control) for 7 days at 30°C and (iii) filtered soil rinse with selected soil bacterial isolates over 7 days. There was a significant difference (P = 0·027) in E. coli O157:H7 counts after 35 days between artificial soils amended with filtered (4·45 ± 0·29) and non‐filtered (1·83 ± 0·33) soil rinse. There were significant differences (P < 0·05) in E. coli O157:H7 counts after 3 days of incubation between soil rinse treatments (heat (7·04 ± 0·03), cycloheximide (6·94 ± 0·05), vancomycin (4·26 ± 0·98) and control (5·00 ± 0·93)). Lastly, a significant difference (P < 0·05) in E. coli O157:H7 counts was observed after 3 days of incubation at 30°C in filtered soil rinse when incubated with Paenibacillus alvei versus other soil bacterial isolates evaluated.
Conclusions
Soil microbiota isolated from Florida sandy soil influenced E. coli O157:H7 survival. Specifically, P. alvei reduced E. coli O157:H7 by over 3 log CFU per ml after 3 days of incubation at 30°C in filtered soil rinse.
Significance and Impact of the Study
This research identified soil bacterial isolates that may reduce E. coli O157:H7 in the soil environment and be used in future biocontrol applications.</abstract><cop>OXFORD</cop><pub>Oxford Univ Press</pub><pmid>33583119</pmid><doi>10.1111/jam.15039</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-9799-9401</orcidid><orcidid>https://orcid.org/0000-0003-0145-3418</orcidid></addata></record> |
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| source | Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | artificial soil Bacteria Biological control Biotechnology & Applied Microbiology Cycloheximide E coli E. coli O157:H7 Escherichia coli Life Sciences & Biomedicine Microbiology Microbiota Paenibacillus Sandy soils Science & Technology Soil amendment Soil bacteria Soil environment Soil investigations Soil microorganisms soil rinse Survival Vancomycin |
| title | Influence of soil microbes on Escherichia coli O157:H7 survival in soil rinse and artificial soil |
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