Lower expression of Hsa_circRNA_102682 in diabetic hyperhomocysteinemia negatively related to creatinemia is associated with TGF‐β and CTGF
Background Diabetic nephropathy is a kidney disease caused by long‐term hyperglycemia. Hsa_circRNA_102682 is related to the pathogenesis of preeclampsia. Preeclampsia is related to hypertension and proteinuria, and diabetic nephropathy is mainly manifested by hypertension and proteinuria. The main p...
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| Veröffentlicht in: | Journal of clinical laboratory analysis 2021-08, Vol.35 (8), p.e23860-n/a, Article 23860 |
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| creator | Hu, Fei Sha, Wenxin Dai, Huixue Yang, Xiangwei Hu, Peng Chu, Yudong Qiu, Xiaohui Bu, Shizhong |
| description | Background
Diabetic nephropathy is a kidney disease caused by long‐term hyperglycemia. Hsa_circRNA_102682 is related to the pathogenesis of preeclampsia. Preeclampsia is related to hypertension and proteinuria, and diabetic nephropathy is mainly manifested by hypertension and proteinuria. The main pathological change in diabetic nephropathy is glomerular fibrosis.
Methods
This study used serum samples of patients treated at Li Huili Eastern Hospital, Ningbo, China, from July 10, 2018 to February 15, 2019. We included 73 patients with diabetes and divided them into a normal‐homocysteine group and a high‐homocysteine group. We selected used quantitative reverse transcriptase‐polymerase chain reaction to measure Hsa_circRNA_102682 concentration in the serum. Serum transforming growth factor‐beta and connective tissue growth factor levels were tested using ELISA. The Pearson correlation test was used to assess the correlations between Hsa_circRNA_102682, transforming growth factor‐beta, connective tissue growth factor, homocysteine, and creatinine.
Result
Hsa_circRNA_102682 was significantly lower in diabetic patients with high levels of homocysteine than in those with normal levels of homocysteine, whereas transforming growth factor‐beta and connective tissue growth factor levels were higher in diabetic patients with hyperhomocysteinemia. Hsa_circRNA_102682 was negatively correlated with the levels of transforming growth factor‐beta, connective tissue growth factor, homocysteine, and creatinine. Transforming growth factor‐beta and connective tissue growth factor were both positively correlated with homocysteine and creatinine.
Conclusion
Low Hsa_circRNA_102682 was associated with high levels of transforming growth factor‐beta and connective tissue growth factor as well as homocysteine and creatinine. These results suggest that Hsa_circRNA_102682 might be related to the pathogenesis of hyperhomocysteinemia in diabetic nephropathy.
Serum levels of Hsa_circRNA_102682 in diabetic patients with normal Hcy (Normal; n = 30) and high Hcy (High; n = 43). |
| doi_str_mv | 10.1002/jcla.23860 |
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| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_journals_2562283524</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2562283524</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4480-f6e263d82353ab001cc6e093d14a34f3299f83e1fad68eefe522f9764d44f703</originalsourceid><addsrcrecordid>eNqNks2O0zAUhSMEYsrAhgdAltigQRn8kzjOBqmK5gdUgYS6t1zneuoqsYudTOmOJ0A8Cw_CQ_AkuNNSAQvEyrLvd8899nGWPSX4nGBMX610p84pExzfyyYE1yKngpb3swkWosoFJuwkexTjCmMsasIfZiesoDWvBJtkX2Z-AwHBp3WAGK13yBt0HZXUNugP76aSYMoFRdah1qoFDFaj5XYNYel7r7dxAOugtwo5uFGDvYVuiwJ0aoAWDR7pAOl0T9iIVIxe27vixg5LNL-6_PH56_dvSLkWNWn3OHtgVBfhyWE9zeaXF_PmOp-9v3rTTGe5LgqBc8OBctYKykqmFhgTrTngmrWkUKwwjNa1EQyIUS0XAAZKSk1d8aItClNhdpq93suux0UPrQY3BNXJdbC9ClvplZV_Vpxdyht_KwWrGONFEnhxEAj-4whxkL2NGrpOOfBjlLQsS0JYUe9mPf8LXfkxuHS7RHFKBSvpTvBsT-ngYwxgjmYIlruU5S5leZdygp_9bv-I_oo1AWIPbGDhTdQWnIYjlv4BrzghIummt2vskDLyrvGjG1Lry_9vTTQ50LaD7T88y7fNbLp3_xOYC9XW</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2562283524</pqid></control><display><type>article</type><title>Lower expression of Hsa_circRNA_102682 in diabetic hyperhomocysteinemia negatively related to creatinemia is associated with TGF‐β and CTGF</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Access via Wiley Online Library</source><source>Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><creator>Hu, Fei ; Sha, Wenxin ; Dai, Huixue ; Yang, Xiangwei ; Hu, Peng ; Chu, Yudong ; Qiu, Xiaohui ; Bu, Shizhong</creator><creatorcontrib>Hu, Fei ; Sha, Wenxin ; Dai, Huixue ; Yang, Xiangwei ; Hu, Peng ; Chu, Yudong ; Qiu, Xiaohui ; Bu, Shizhong</creatorcontrib><description>Background
Diabetic nephropathy is a kidney disease caused by long‐term hyperglycemia. Hsa_circRNA_102682 is related to the pathogenesis of preeclampsia. Preeclampsia is related to hypertension and proteinuria, and diabetic nephropathy is mainly manifested by hypertension and proteinuria. The main pathological change in diabetic nephropathy is glomerular fibrosis.
Methods
This study used serum samples of patients treated at Li Huili Eastern Hospital, Ningbo, China, from July 10, 2018 to February 15, 2019. We included 73 patients with diabetes and divided them into a normal‐homocysteine group and a high‐homocysteine group. We selected used quantitative reverse transcriptase‐polymerase chain reaction to measure Hsa_circRNA_102682 concentration in the serum. Serum transforming growth factor‐beta and connective tissue growth factor levels were tested using ELISA. The Pearson correlation test was used to assess the correlations between Hsa_circRNA_102682, transforming growth factor‐beta, connective tissue growth factor, homocysteine, and creatinine.
Result
Hsa_circRNA_102682 was significantly lower in diabetic patients with high levels of homocysteine than in those with normal levels of homocysteine, whereas transforming growth factor‐beta and connective tissue growth factor levels were higher in diabetic patients with hyperhomocysteinemia. Hsa_circRNA_102682 was negatively correlated with the levels of transforming growth factor‐beta, connective tissue growth factor, homocysteine, and creatinine. Transforming growth factor‐beta and connective tissue growth factor were both positively correlated with homocysteine and creatinine.
Conclusion
Low Hsa_circRNA_102682 was associated with high levels of transforming growth factor‐beta and connective tissue growth factor as well as homocysteine and creatinine. These results suggest that Hsa_circRNA_102682 might be related to the pathogenesis of hyperhomocysteinemia in diabetic nephropathy.
Serum levels of Hsa_circRNA_102682 in diabetic patients with normal Hcy (Normal; n = 30) and high Hcy (High; n = 43).</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.23860</identifier><identifier>PMID: 34296783</identifier><language>eng</language><publisher>HOBOKEN: Wiley</publisher><subject>Alzheimer's disease ; Biomarkers ; Blood pressure ; Body mass index ; circular RNA ; Connective tissue growth factor ; Connective Tissue Growth Factor - blood ; Connective tissues ; Correlation analysis ; Creatinine ; Creatinine - blood ; CTGF ; Diabetes ; Diabetes mellitus ; Diabetic Nephropathies - blood ; Diabetic Nephropathies - diagnosis ; Diabetic Nephropathies - genetics ; Diabetic nephropathy ; Fibrosis ; Gene Expression Regulation ; Glucose ; Growth factors ; Hemoglobin ; Homocysteine ; Homocysteine - blood ; Homocysteine - genetics ; Hospitals ; Hsa_circRNA_102682 ; Humans ; Hyperglycemia ; Hyperhomocysteinemia ; Hyperhomocysteinemia - blood ; Hyperhomocysteinemia - genetics ; Hypertension ; Kidney diseases ; Life Sciences & Biomedicine ; Lipoproteins ; Medical Laboratory Technology ; Middle Aged ; Nephropathy ; Normal distribution ; Patients ; Peptides ; Polymerase chain reaction ; Pre-eclampsia ; Proteinuria ; RNA, Circular - blood ; RNA-directed DNA polymerase ; ROC Curve ; Science & Technology ; TGF‐β ; Transforming Growth Factor beta - blood ; Transforming growth factor-b ; Urine</subject><ispartof>Journal of clinical laboratory analysis, 2021-08, Vol.35 (8), p.e23860-n/a, Article 23860</ispartof><rights>2021 The Authors. published by Wiley Periodicals LLC</rights><rights>2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>4</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000676118100001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c4480-f6e263d82353ab001cc6e093d14a34f3299f83e1fad68eefe522f9764d44f703</citedby><cites>FETCH-LOGICAL-c4480-f6e263d82353ab001cc6e093d14a34f3299f83e1fad68eefe522f9764d44f703</cites><orcidid>0000-0001-5196-6030</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373364/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373364/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,1418,2115,11567,27929,27930,39263,45579,45580,46057,46481,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34296783$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Fei</creatorcontrib><creatorcontrib>Sha, Wenxin</creatorcontrib><creatorcontrib>Dai, Huixue</creatorcontrib><creatorcontrib>Yang, Xiangwei</creatorcontrib><creatorcontrib>Hu, Peng</creatorcontrib><creatorcontrib>Chu, Yudong</creatorcontrib><creatorcontrib>Qiu, Xiaohui</creatorcontrib><creatorcontrib>Bu, Shizhong</creatorcontrib><title>Lower expression of Hsa_circRNA_102682 in diabetic hyperhomocysteinemia negatively related to creatinemia is associated with TGF‐β and CTGF</title><title>Journal of clinical laboratory analysis</title><addtitle>J CLIN LAB ANAL</addtitle><addtitle>J Clin Lab Anal</addtitle><description>Background
Diabetic nephropathy is a kidney disease caused by long‐term hyperglycemia. Hsa_circRNA_102682 is related to the pathogenesis of preeclampsia. Preeclampsia is related to hypertension and proteinuria, and diabetic nephropathy is mainly manifested by hypertension and proteinuria. The main pathological change in diabetic nephropathy is glomerular fibrosis.
Methods
This study used serum samples of patients treated at Li Huili Eastern Hospital, Ningbo, China, from July 10, 2018 to February 15, 2019. We included 73 patients with diabetes and divided them into a normal‐homocysteine group and a high‐homocysteine group. We selected used quantitative reverse transcriptase‐polymerase chain reaction to measure Hsa_circRNA_102682 concentration in the serum. Serum transforming growth factor‐beta and connective tissue growth factor levels were tested using ELISA. The Pearson correlation test was used to assess the correlations between Hsa_circRNA_102682, transforming growth factor‐beta, connective tissue growth factor, homocysteine, and creatinine.
Result
Hsa_circRNA_102682 was significantly lower in diabetic patients with high levels of homocysteine than in those with normal levels of homocysteine, whereas transforming growth factor‐beta and connective tissue growth factor levels were higher in diabetic patients with hyperhomocysteinemia. Hsa_circRNA_102682 was negatively correlated with the levels of transforming growth factor‐beta, connective tissue growth factor, homocysteine, and creatinine. Transforming growth factor‐beta and connective tissue growth factor were both positively correlated with homocysteine and creatinine.
Conclusion
Low Hsa_circRNA_102682 was associated with high levels of transforming growth factor‐beta and connective tissue growth factor as well as homocysteine and creatinine. These results suggest that Hsa_circRNA_102682 might be related to the pathogenesis of hyperhomocysteinemia in diabetic nephropathy.
Serum levels of Hsa_circRNA_102682 in diabetic patients with normal Hcy (Normal; n = 30) and high Hcy (High; n = 43).</description><subject>Alzheimer's disease</subject><subject>Biomarkers</subject><subject>Blood pressure</subject><subject>Body mass index</subject><subject>circular RNA</subject><subject>Connective tissue growth factor</subject><subject>Connective Tissue Growth Factor - blood</subject><subject>Connective tissues</subject><subject>Correlation analysis</subject><subject>Creatinine</subject><subject>Creatinine - blood</subject><subject>CTGF</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetic Nephropathies - blood</subject><subject>Diabetic Nephropathies - diagnosis</subject><subject>Diabetic Nephropathies - genetics</subject><subject>Diabetic nephropathy</subject><subject>Fibrosis</subject><subject>Gene Expression Regulation</subject><subject>Glucose</subject><subject>Growth factors</subject><subject>Hemoglobin</subject><subject>Homocysteine</subject><subject>Homocysteine - blood</subject><subject>Homocysteine - genetics</subject><subject>Hospitals</subject><subject>Hsa_circRNA_102682</subject><subject>Humans</subject><subject>Hyperglycemia</subject><subject>Hyperhomocysteinemia</subject><subject>Hyperhomocysteinemia - blood</subject><subject>Hyperhomocysteinemia - genetics</subject><subject>Hypertension</subject><subject>Kidney diseases</subject><subject>Life Sciences & Biomedicine</subject><subject>Lipoproteins</subject><subject>Medical Laboratory Technology</subject><subject>Middle Aged</subject><subject>Nephropathy</subject><subject>Normal distribution</subject><subject>Patients</subject><subject>Peptides</subject><subject>Polymerase chain reaction</subject><subject>Pre-eclampsia</subject><subject>Proteinuria</subject><subject>RNA, Circular - blood</subject><subject>RNA-directed DNA polymerase</subject><subject>ROC Curve</subject><subject>Science & Technology</subject><subject>TGF‐β</subject><subject>Transforming Growth Factor beta - blood</subject><subject>Transforming growth factor-b</subject><subject>Urine</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks2O0zAUhSMEYsrAhgdAltigQRn8kzjOBqmK5gdUgYS6t1zneuoqsYudTOmOJ0A8Cw_CQ_AkuNNSAQvEyrLvd8899nGWPSX4nGBMX610p84pExzfyyYE1yKngpb3swkWosoFJuwkexTjCmMsasIfZiesoDWvBJtkX2Z-AwHBp3WAGK13yBt0HZXUNugP76aSYMoFRdah1qoFDFaj5XYNYel7r7dxAOugtwo5uFGDvYVuiwJ0aoAWDR7pAOl0T9iIVIxe27vixg5LNL-6_PH56_dvSLkWNWn3OHtgVBfhyWE9zeaXF_PmOp-9v3rTTGe5LgqBc8OBctYKykqmFhgTrTngmrWkUKwwjNa1EQyIUS0XAAZKSk1d8aItClNhdpq93suux0UPrQY3BNXJdbC9ClvplZV_Vpxdyht_KwWrGONFEnhxEAj-4whxkL2NGrpOOfBjlLQsS0JYUe9mPf8LXfkxuHS7RHFKBSvpTvBsT-ngYwxgjmYIlruU5S5leZdygp_9bv-I_oo1AWIPbGDhTdQWnIYjlv4BrzghIummt2vskDLyrvGjG1Lry_9vTTQ50LaD7T88y7fNbLp3_xOYC9XW</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Hu, Fei</creator><creator>Sha, Wenxin</creator><creator>Dai, Huixue</creator><creator>Yang, Xiangwei</creator><creator>Hu, Peng</creator><creator>Chu, Yudong</creator><creator>Qiu, Xiaohui</creator><creator>Bu, Shizhong</creator><general>Wiley</general><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5196-6030</orcidid></search><sort><creationdate>202108</creationdate><title>Lower expression of Hsa_circRNA_102682 in diabetic hyperhomocysteinemia negatively related to creatinemia is associated with TGF‐β and CTGF</title><author>Hu, Fei ; Sha, Wenxin ; Dai, Huixue ; Yang, Xiangwei ; Hu, Peng ; Chu, Yudong ; Qiu, Xiaohui ; Bu, Shizhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4480-f6e263d82353ab001cc6e093d14a34f3299f83e1fad68eefe522f9764d44f703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alzheimer's disease</topic><topic>Biomarkers</topic><topic>Blood pressure</topic><topic>Body mass index</topic><topic>circular RNA</topic><topic>Connective tissue growth factor</topic><topic>Connective Tissue Growth Factor - blood</topic><topic>Connective tissues</topic><topic>Correlation analysis</topic><topic>Creatinine</topic><topic>Creatinine - blood</topic><topic>CTGF</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetic Nephropathies - blood</topic><topic>Diabetic Nephropathies - diagnosis</topic><topic>Diabetic Nephropathies - genetics</topic><topic>Diabetic nephropathy</topic><topic>Fibrosis</topic><topic>Gene Expression Regulation</topic><topic>Glucose</topic><topic>Growth factors</topic><topic>Hemoglobin</topic><topic>Homocysteine</topic><topic>Homocysteine - blood</topic><topic>Homocysteine - genetics</topic><topic>Hospitals</topic><topic>Hsa_circRNA_102682</topic><topic>Humans</topic><topic>Hyperglycemia</topic><topic>Hyperhomocysteinemia</topic><topic>Hyperhomocysteinemia - blood</topic><topic>Hyperhomocysteinemia - genetics</topic><topic>Hypertension</topic><topic>Kidney diseases</topic><topic>Life Sciences & Biomedicine</topic><topic>Lipoproteins</topic><topic>Medical Laboratory Technology</topic><topic>Middle Aged</topic><topic>Nephropathy</topic><topic>Normal distribution</topic><topic>Patients</topic><topic>Peptides</topic><topic>Polymerase chain reaction</topic><topic>Pre-eclampsia</topic><topic>Proteinuria</topic><topic>RNA, Circular - blood</topic><topic>RNA-directed DNA polymerase</topic><topic>ROC Curve</topic><topic>Science & Technology</topic><topic>TGF‐β</topic><topic>Transforming Growth Factor beta - blood</topic><topic>Transforming growth factor-b</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Fei</creatorcontrib><creatorcontrib>Sha, Wenxin</creatorcontrib><creatorcontrib>Dai, Huixue</creatorcontrib><creatorcontrib>Yang, Xiangwei</creatorcontrib><creatorcontrib>Hu, Peng</creatorcontrib><creatorcontrib>Chu, Yudong</creatorcontrib><creatorcontrib>Qiu, Xiaohui</creatorcontrib><creatorcontrib>Bu, Shizhong</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Fei</au><au>Sha, Wenxin</au><au>Dai, Huixue</au><au>Yang, Xiangwei</au><au>Hu, Peng</au><au>Chu, Yudong</au><au>Qiu, Xiaohui</au><au>Bu, Shizhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lower expression of Hsa_circRNA_102682 in diabetic hyperhomocysteinemia negatively related to creatinemia is associated with TGF‐β and CTGF</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><stitle>J CLIN LAB ANAL</stitle><addtitle>J Clin Lab Anal</addtitle><date>2021-08</date><risdate>2021</risdate><volume>35</volume><issue>8</issue><spage>e23860</spage><epage>n/a</epage><pages>e23860-n/a</pages><artnum>23860</artnum><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>Background
Diabetic nephropathy is a kidney disease caused by long‐term hyperglycemia. Hsa_circRNA_102682 is related to the pathogenesis of preeclampsia. Preeclampsia is related to hypertension and proteinuria, and diabetic nephropathy is mainly manifested by hypertension and proteinuria. The main pathological change in diabetic nephropathy is glomerular fibrosis.
Methods
This study used serum samples of patients treated at Li Huili Eastern Hospital, Ningbo, China, from July 10, 2018 to February 15, 2019. We included 73 patients with diabetes and divided them into a normal‐homocysteine group and a high‐homocysteine group. We selected used quantitative reverse transcriptase‐polymerase chain reaction to measure Hsa_circRNA_102682 concentration in the serum. Serum transforming growth factor‐beta and connective tissue growth factor levels were tested using ELISA. The Pearson correlation test was used to assess the correlations between Hsa_circRNA_102682, transforming growth factor‐beta, connective tissue growth factor, homocysteine, and creatinine.
Result
Hsa_circRNA_102682 was significantly lower in diabetic patients with high levels of homocysteine than in those with normal levels of homocysteine, whereas transforming growth factor‐beta and connective tissue growth factor levels were higher in diabetic patients with hyperhomocysteinemia. Hsa_circRNA_102682 was negatively correlated with the levels of transforming growth factor‐beta, connective tissue growth factor, homocysteine, and creatinine. Transforming growth factor‐beta and connective tissue growth factor were both positively correlated with homocysteine and creatinine.
Conclusion
Low Hsa_circRNA_102682 was associated with high levels of transforming growth factor‐beta and connective tissue growth factor as well as homocysteine and creatinine. These results suggest that Hsa_circRNA_102682 might be related to the pathogenesis of hyperhomocysteinemia in diabetic nephropathy.
Serum levels of Hsa_circRNA_102682 in diabetic patients with normal Hcy (Normal; n = 30) and high Hcy (High; n = 43).</abstract><cop>HOBOKEN</cop><pub>Wiley</pub><pmid>34296783</pmid><doi>10.1002/jcla.23860</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-5196-6030</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of clinical laboratory analysis, 2021-08, Vol.35 (8), p.e23860-n/a, Article 23860 |
| issn | 0887-8013 1098-2825 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Access via Wiley Online Library; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central |
| subjects | Alzheimer's disease Biomarkers Blood pressure Body mass index circular RNA Connective tissue growth factor Connective Tissue Growth Factor - blood Connective tissues Correlation analysis Creatinine Creatinine - blood CTGF Diabetes Diabetes mellitus Diabetic Nephropathies - blood Diabetic Nephropathies - diagnosis Diabetic Nephropathies - genetics Diabetic nephropathy Fibrosis Gene Expression Regulation Glucose Growth factors Hemoglobin Homocysteine Homocysteine - blood Homocysteine - genetics Hospitals Hsa_circRNA_102682 Humans Hyperglycemia Hyperhomocysteinemia Hyperhomocysteinemia - blood Hyperhomocysteinemia - genetics Hypertension Kidney diseases Life Sciences & Biomedicine Lipoproteins Medical Laboratory Technology Middle Aged Nephropathy Normal distribution Patients Peptides Polymerase chain reaction Pre-eclampsia Proteinuria RNA, Circular - blood RNA-directed DNA polymerase ROC Curve Science & Technology TGF‐β Transforming Growth Factor beta - blood Transforming growth factor-b Urine |
| title | Lower expression of Hsa_circRNA_102682 in diabetic hyperhomocysteinemia negatively related to creatinemia is associated with TGF‐β and CTGF |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T06%3A14%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lower%20expression%20of%20Hsa_circRNA_102682%20in%20diabetic%20hyperhomocysteinemia%20negatively%20related%20to%20creatinemia%20is%20associated%20with%20TGF%E2%80%90%CE%B2%20and%20CTGF&rft.jtitle=Journal%20of%20clinical%20laboratory%20analysis&rft.au=Hu,%20Fei&rft.date=2021-08&rft.volume=35&rft.issue=8&rft.spage=e23860&rft.epage=n/a&rft.pages=e23860-n/a&rft.artnum=23860&rft.issn=0887-8013&rft.eissn=1098-2825&rft_id=info:doi/10.1002/jcla.23860&rft_dat=%3Cproquest_pubme%3E2562283524%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2562283524&rft_id=info:pmid/34296783&rfr_iscdi=true |