The therapeutic effects of Prunella vulgaris against fluoride‐induced oxidative damage by using the metabolomics method
Fluoride is considered as one of the most ubiquitous environmental pollutants. Numerous studies have linked reactive oxygen species (ROS)‐dependent oxidative damage with fluoride intoxication, which could be prevented by antioxidants. However, the metabolomic changes induced by ROS disruptions in fl...
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| Veröffentlicht in: | Environmental toxicology 2021-09, Vol.36 (9), p.1802-1816 |
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| creator | Li, Li Lin, Li‐mei Deng, Jing Lin, Xiu‐lian Li, Ya‐mei Xia, Bo‐hou |
| description | Fluoride is considered as one of the most ubiquitous environmental pollutants. Numerous studies have linked reactive oxygen species (ROS)‐dependent oxidative damage with fluoride intoxication, which could be prevented by antioxidants. However, the metabolomic changes induced by ROS disruptions in fluoride intoxication are yet unknown. The present study aimed to provide novel mechanistic insights into the fluoride‐induced oxidative damage and to investigate the potential protective effects of ethanolic extract of Prunella vulgaris (natural antioxidant, PV) against fluoride‐induced oxidative damage. The serum biochemical indicators related to fluoride‐induced oxidative damage, such as lipid peroxidation parameter, inflammation and marker enzymes in the liver increased significantly in the fluoride‐treated group, while antioxidant enzymes were decreased. However, PV treatment restored the level of these biochemical indicators, indicating satisfactory antioxidant, anti‐inflammatory, and hepatoprotective potential of PV. The metabolomics analysis in the serum was performed by liquid chromatography–mass spectroscopy, whereas the fluoride treatment caused severe metabolic disorders in rats, which could be improved by PV. The differential metabolites screened by multivariate analysis after fluoride and PV treatment, were organic acids, fatty acids, and lipids. These differential metabolites represented disorders of glyoxylate and dicarboxylate metabolism and the citrate cycle (TCA) according to metabolic pathway analysis in fluoride treatment rats. Interestingly, the result of metabolic pathway analysis of post‐treatment with PV was consistent with that of fluoride treatment, indicating that the energy metabolism plays a major role in the progress of fluoride‐induced oxidative damage, as well as the therapeutic effect of PV. These findings provided a theoretical basis for understanding the mechanism underlying metabolic disorders of fluoride toxicity and the effect of PV. |
| doi_str_mv | 10.1002/tox.23301 |
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Numerous studies have linked reactive oxygen species (ROS)‐dependent oxidative damage with fluoride intoxication, which could be prevented by antioxidants. However, the metabolomic changes induced by ROS disruptions in fluoride intoxication are yet unknown. The present study aimed to provide novel mechanistic insights into the fluoride‐induced oxidative damage and to investigate the potential protective effects of ethanolic extract of Prunella vulgaris (natural antioxidant, PV) against fluoride‐induced oxidative damage. The serum biochemical indicators related to fluoride‐induced oxidative damage, such as lipid peroxidation parameter, inflammation and marker enzymes in the liver increased significantly in the fluoride‐treated group, while antioxidant enzymes were decreased. However, PV treatment restored the level of these biochemical indicators, indicating satisfactory antioxidant, anti‐inflammatory, and hepatoprotective potential of PV. The metabolomics analysis in the serum was performed by liquid chromatography–mass spectroscopy, whereas the fluoride treatment caused severe metabolic disorders in rats, which could be improved by PV. The differential metabolites screened by multivariate analysis after fluoride and PV treatment, were organic acids, fatty acids, and lipids. These differential metabolites represented disorders of glyoxylate and dicarboxylate metabolism and the citrate cycle (TCA) according to metabolic pathway analysis in fluoride treatment rats. Interestingly, the result of metabolic pathway analysis of post‐treatment with PV was consistent with that of fluoride treatment, indicating that the energy metabolism plays a major role in the progress of fluoride‐induced oxidative damage, as well as the therapeutic effect of PV. These findings provided a theoretical basis for understanding the mechanism underlying metabolic disorders of fluoride toxicity and the effect of PV.</description><identifier>ISSN: 1520-4081</identifier><identifier>EISSN: 1522-7278</identifier><identifier>DOI: 10.1002/tox.23301</identifier><identifier>PMID: 34089294</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Analytical methods ; Antioxidants ; Citric acid ; Damage ; Energy metabolism ; Environmental Sciences ; Environmental Sciences & Ecology ; Enzymes ; Fatty acids ; fluoride ; Fluorides ; Indicators ; Inflammation ; Intoxication ; Life Sciences & Biomedicine ; Lipid metabolism ; Lipid peroxidation ; Lipids ; Liquid chromatography ; Mass spectroscopy ; Metabolic disorders ; Metabolic pathways ; Metabolites ; Metabolomics ; Multivariate analysis ; Organic acids ; oxidative damage ; Peroxidation ; Physical Sciences ; Pollutants ; Prunella vulgaris ; Reactive oxygen species ; Science & Technology ; Serum ; Toxicity ; Toxicology ; Water Resources</subject><ispartof>Environmental toxicology, 2021-09, Vol.36 (9), p.1802-1816</ispartof><rights>2021 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>15</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000658043500001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c3301-d8b55b87ead9b6fe457a4e1f495cbea08a7e42c8c168ae4b734a16e43ab6abe63</citedby><cites>FETCH-LOGICAL-c3301-d8b55b87ead9b6fe457a4e1f495cbea08a7e42c8c168ae4b734a16e43ab6abe63</cites><orcidid>0000-0003-0570-2995</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Ftox.23301$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Ftox.23301$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,39263,45579,45580</link.rule.ids></links><search><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Lin, Li‐mei</creatorcontrib><creatorcontrib>Deng, Jing</creatorcontrib><creatorcontrib>Lin, Xiu‐lian</creatorcontrib><creatorcontrib>Li, Ya‐mei</creatorcontrib><creatorcontrib>Xia, Bo‐hou</creatorcontrib><title>The therapeutic effects of Prunella vulgaris against fluoride‐induced oxidative damage by using the metabolomics method</title><title>Environmental toxicology</title><addtitle>ENVIRON TOXICOL</addtitle><description>Fluoride is considered as one of the most ubiquitous environmental pollutants. Numerous studies have linked reactive oxygen species (ROS)‐dependent oxidative damage with fluoride intoxication, which could be prevented by antioxidants. However, the metabolomic changes induced by ROS disruptions in fluoride intoxication are yet unknown. The present study aimed to provide novel mechanistic insights into the fluoride‐induced oxidative damage and to investigate the potential protective effects of ethanolic extract of Prunella vulgaris (natural antioxidant, PV) against fluoride‐induced oxidative damage. The serum biochemical indicators related to fluoride‐induced oxidative damage, such as lipid peroxidation parameter, inflammation and marker enzymes in the liver increased significantly in the fluoride‐treated group, while antioxidant enzymes were decreased. However, PV treatment restored the level of these biochemical indicators, indicating satisfactory antioxidant, anti‐inflammatory, and hepatoprotective potential of PV. The metabolomics analysis in the serum was performed by liquid chromatography–mass spectroscopy, whereas the fluoride treatment caused severe metabolic disorders in rats, which could be improved by PV. The differential metabolites screened by multivariate analysis after fluoride and PV treatment, were organic acids, fatty acids, and lipids. These differential metabolites represented disorders of glyoxylate and dicarboxylate metabolism and the citrate cycle (TCA) according to metabolic pathway analysis in fluoride treatment rats. Interestingly, the result of metabolic pathway analysis of post‐treatment with PV was consistent with that of fluoride treatment, indicating that the energy metabolism plays a major role in the progress of fluoride‐induced oxidative damage, as well as the therapeutic effect of PV. These findings provided a theoretical basis for understanding the mechanism underlying metabolic disorders of fluoride toxicity and the effect of PV.</description><subject>Analytical methods</subject><subject>Antioxidants</subject><subject>Citric acid</subject><subject>Damage</subject><subject>Energy metabolism</subject><subject>Environmental Sciences</subject><subject>Environmental Sciences & Ecology</subject><subject>Enzymes</subject><subject>Fatty acids</subject><subject>fluoride</subject><subject>Fluorides</subject><subject>Indicators</subject><subject>Inflammation</subject><subject>Intoxication</subject><subject>Life Sciences & Biomedicine</subject><subject>Lipid metabolism</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Liquid chromatography</subject><subject>Mass spectroscopy</subject><subject>Metabolic disorders</subject><subject>Metabolic pathways</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Multivariate analysis</subject><subject>Organic acids</subject><subject>oxidative damage</subject><subject>Peroxidation</subject><subject>Physical Sciences</subject><subject>Pollutants</subject><subject>Prunella vulgaris</subject><subject>Reactive oxygen species</subject><subject>Science & Technology</subject><subject>Serum</subject><subject>Toxicity</subject><subject>Toxicology</subject><subject>Water Resources</subject><issn>1520-4081</issn><issn>1522-7278</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><recordid>eNqNkM1q3DAQgEVpaNK0h76BoKdSnEi2ZMvHsvQnEEgOW-jNjOTRroLX2uonyd76CH3GPkm1uyG3QnXRaPhmNPMR8o6zC85YfZn840XdNIy_IGdc1nXV1Z16eYhZJZjip-R1jHeMsb6V7Sty2pRkX_fijOyWa6RpjQG2mJMzFK1FkyL1lt6GPOM0Ab3P0wqCixRW4OaYqJ2yD27EP79-u3nMBkfqH90Iyd0jHWEDK6R6R3N082rfnW4wgfaT3zgT94-1H9-QEwtTxLdP9zn5_uXzcvGtur75erX4dF2Z_UbVqLSUWnUIY69bi0J2IJBb0UujEZiCDkVtlOGtAhS6awTwFkUDugWNbXNO3h_7boP_mTGm4c7nMJcvh1rKvpxOdIX6cKRM8DEGtMM2uA2E3cDZsJc8FMnDQXJhPx7ZB9TeRuNwNvjMF8utVEw0skQHWv0_vXCpSPTzwuc5ldLLp1I34e7fEw3Lmx_H0f4ClzmhGw</recordid><startdate>202109</startdate><enddate>202109</enddate><creator>Li, Li</creator><creator>Lin, Li‐mei</creator><creator>Deng, Jing</creator><creator>Lin, Xiu‐lian</creator><creator>Li, Ya‐mei</creator><creator>Xia, Bo‐hou</creator><general>John Wiley & Sons, Inc</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M7N</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0003-0570-2995</orcidid></search><sort><creationdate>202109</creationdate><title>The therapeutic effects of Prunella vulgaris against fluoride‐induced oxidative damage by using the metabolomics method</title><author>Li, Li ; Lin, Li‐mei ; Deng, Jing ; Lin, Xiu‐lian ; Li, Ya‐mei ; Xia, Bo‐hou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3301-d8b55b87ead9b6fe457a4e1f495cbea08a7e42c8c168ae4b734a16e43ab6abe63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analytical methods</topic><topic>Antioxidants</topic><topic>Citric acid</topic><topic>Damage</topic><topic>Energy metabolism</topic><topic>Environmental Sciences</topic><topic>Environmental Sciences & Ecology</topic><topic>Enzymes</topic><topic>Fatty acids</topic><topic>fluoride</topic><topic>Fluorides</topic><topic>Indicators</topic><topic>Inflammation</topic><topic>Intoxication</topic><topic>Life Sciences & Biomedicine</topic><topic>Lipid metabolism</topic><topic>Lipid peroxidation</topic><topic>Lipids</topic><topic>Liquid chromatography</topic><topic>Mass spectroscopy</topic><topic>Metabolic disorders</topic><topic>Metabolic pathways</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Multivariate analysis</topic><topic>Organic acids</topic><topic>oxidative damage</topic><topic>Peroxidation</topic><topic>Physical Sciences</topic><topic>Pollutants</topic><topic>Prunella vulgaris</topic><topic>Reactive oxygen species</topic><topic>Science & Technology</topic><topic>Serum</topic><topic>Toxicity</topic><topic>Toxicology</topic><topic>Water Resources</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Lin, Li‐mei</creatorcontrib><creatorcontrib>Deng, Jing</creatorcontrib><creatorcontrib>Lin, Xiu‐lian</creatorcontrib><creatorcontrib>Li, Ya‐mei</creatorcontrib><creatorcontrib>Xia, Bo‐hou</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Environment Abstracts</collection><jtitle>Environmental toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Li</au><au>Lin, Li‐mei</au><au>Deng, Jing</au><au>Lin, Xiu‐lian</au><au>Li, Ya‐mei</au><au>Xia, Bo‐hou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The therapeutic effects of Prunella vulgaris against fluoride‐induced oxidative damage by using the metabolomics method</atitle><jtitle>Environmental toxicology</jtitle><stitle>ENVIRON TOXICOL</stitle><date>2021-09</date><risdate>2021</risdate><volume>36</volume><issue>9</issue><spage>1802</spage><epage>1816</epage><pages>1802-1816</pages><issn>1520-4081</issn><eissn>1522-7278</eissn><abstract>Fluoride is considered as one of the most ubiquitous environmental pollutants. Numerous studies have linked reactive oxygen species (ROS)‐dependent oxidative damage with fluoride intoxication, which could be prevented by antioxidants. However, the metabolomic changes induced by ROS disruptions in fluoride intoxication are yet unknown. The present study aimed to provide novel mechanistic insights into the fluoride‐induced oxidative damage and to investigate the potential protective effects of ethanolic extract of Prunella vulgaris (natural antioxidant, PV) against fluoride‐induced oxidative damage. The serum biochemical indicators related to fluoride‐induced oxidative damage, such as lipid peroxidation parameter, inflammation and marker enzymes in the liver increased significantly in the fluoride‐treated group, while antioxidant enzymes were decreased. However, PV treatment restored the level of these biochemical indicators, indicating satisfactory antioxidant, anti‐inflammatory, and hepatoprotective potential of PV. The metabolomics analysis in the serum was performed by liquid chromatography–mass spectroscopy, whereas the fluoride treatment caused severe metabolic disorders in rats, which could be improved by PV. The differential metabolites screened by multivariate analysis after fluoride and PV treatment, were organic acids, fatty acids, and lipids. These differential metabolites represented disorders of glyoxylate and dicarboxylate metabolism and the citrate cycle (TCA) according to metabolic pathway analysis in fluoride treatment rats. Interestingly, the result of metabolic pathway analysis of post‐treatment with PV was consistent with that of fluoride treatment, indicating that the energy metabolism plays a major role in the progress of fluoride‐induced oxidative damage, as well as the therapeutic effect of PV. These findings provided a theoretical basis for understanding the mechanism underlying metabolic disorders of fluoride toxicity and the effect of PV.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>34089294</pmid><doi>10.1002/tox.23301</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-0570-2995</orcidid></addata></record> |
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| subjects | Analytical methods Antioxidants Citric acid Damage Energy metabolism Environmental Sciences Environmental Sciences & Ecology Enzymes Fatty acids fluoride Fluorides Indicators Inflammation Intoxication Life Sciences & Biomedicine Lipid metabolism Lipid peroxidation Lipids Liquid chromatography Mass spectroscopy Metabolic disorders Metabolic pathways Metabolites Metabolomics Multivariate analysis Organic acids oxidative damage Peroxidation Physical Sciences Pollutants Prunella vulgaris Reactive oxygen species Science & Technology Serum Toxicity Toxicology Water Resources |
| title | The therapeutic effects of Prunella vulgaris against fluoride‐induced oxidative damage by using the metabolomics method |
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