Alternative medium from agricultural by-products used for a starter of vinegar fermentation
The objective of this research was to find a cheap agricultural waste media for the preparation of the effective starter culture of acetic acid bacteria (AAB). The alternative culture medium for AAB was compared to potato medium, preparing from pineapple peel extract (PPE) and banana juice. These me...
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| Veröffentlicht in: | IOP conference series. Earth and environmental science 2019-08, Vol.301 (1), p.12035 |
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| creator | Tanamool, V Soemphol, W |
| description | The objective of this research was to find a cheap agricultural waste media for the preparation of the effective starter culture of acetic acid bacteria (AAB). The alternative culture medium for AAB was compared to potato medium, preparing from pineapple peel extract (PPE) and banana juice. These media was adjusted the reducing sugar to 25 gL−1 and initial pH of 5.0 used for growing Acetobacter pasteurianus FPB2-3. The results found that this strain grew well in PPE medium comparable to potato medium and viable cells achieved the level of at least 9 log CFUmL−1 when cultured for 36 hr. While addition of banana juice, led cell growth decreased. The highest alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities in cell free extract were gained by using potato medium while the alternative media showed decrease of activity. However, these pre-culture gave acetic acid production same as the control medium, which cell completely oxidize ethanol to acetic acid. Consistently, analysis by FT-IR indicated insignificantly difference on cell components among these media. The result shows that constitutes of such agricultural by-product can be utilized as an alternative cheap inoculation media for AAB. |
| doi_str_mv | 10.1088/1755-1315/301/1/012035 |
| format | Article |
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The alternative culture medium for AAB was compared to potato medium, preparing from pineapple peel extract (PPE) and banana juice. These media was adjusted the reducing sugar to 25 gL−1 and initial pH of 5.0 used for growing Acetobacter pasteurianus FPB2-3. The results found that this strain grew well in PPE medium comparable to potato medium and viable cells achieved the level of at least 9 log CFUmL−1 when cultured for 36 hr. While addition of banana juice, led cell growth decreased. The highest alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities in cell free extract were gained by using potato medium while the alternative media showed decrease of activity. However, these pre-culture gave acetic acid production same as the control medium, which cell completely oxidize ethanol to acetic acid. Consistently, analysis by FT-IR indicated insignificantly difference on cell components among these media. The result shows that constitutes of such agricultural by-product can be utilized as an alternative cheap inoculation media for AAB.</description><identifier>ISSN: 1755-1307</identifier><identifier>EISSN: 1755-1315</identifier><identifier>DOI: 10.1088/1755-1315/301/1/012035</identifier><language>eng</language><publisher>Bristol: IOP Publishing</publisher><subject>Acetaldehyde ; Acetaldehyde dehydrogenase ; Acetic acid ; Acetic acid bacteria ; Acid production ; Agricultural wastes ; Alcohol dehydrogenase ; Bacteria ; Byproducts ; Cell culture ; Dehydrogenase ; Dehydrogenases ; Ethanol ; Fermentation ; Infrared analysis ; Inoculation ; Media ; Plant extracts ; Potatoes ; Starter cultures ; Vinegar</subject><ispartof>IOP conference series. Earth and environmental science, 2019-08, Vol.301 (1), p.12035</ispartof><rights>Published under licence by IOP Publishing Ltd</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). 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However, these pre-culture gave acetic acid production same as the control medium, which cell completely oxidize ethanol to acetic acid. Consistently, analysis by FT-IR indicated insignificantly difference on cell components among these media. 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Earth and environmental science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanamool, V</au><au>Soemphol, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alternative medium from agricultural by-products used for a starter of vinegar fermentation</atitle><jtitle>IOP conference series. Earth and environmental science</jtitle><addtitle>IOP Conf. Ser.: Earth Environ. Sci</addtitle><date>2019-08-01</date><risdate>2019</risdate><volume>301</volume><issue>1</issue><spage>12035</spage><pages>12035-</pages><issn>1755-1307</issn><eissn>1755-1315</eissn><abstract>The objective of this research was to find a cheap agricultural waste media for the preparation of the effective starter culture of acetic acid bacteria (AAB). The alternative culture medium for AAB was compared to potato medium, preparing from pineapple peel extract (PPE) and banana juice. These media was adjusted the reducing sugar to 25 gL−1 and initial pH of 5.0 used for growing Acetobacter pasteurianus FPB2-3. The results found that this strain grew well in PPE medium comparable to potato medium and viable cells achieved the level of at least 9 log CFUmL−1 when cultured for 36 hr. While addition of banana juice, led cell growth decreased. The highest alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities in cell free extract were gained by using potato medium while the alternative media showed decrease of activity. However, these pre-culture gave acetic acid production same as the control medium, which cell completely oxidize ethanol to acetic acid. Consistently, analysis by FT-IR indicated insignificantly difference on cell components among these media. 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| subjects | Acetaldehyde Acetaldehyde dehydrogenase Acetic acid Acetic acid bacteria Acid production Agricultural wastes Alcohol dehydrogenase Bacteria Byproducts Cell culture Dehydrogenase Dehydrogenases Ethanol Fermentation Infrared analysis Inoculation Media Plant extracts Potatoes Starter cultures Vinegar |
| title | Alternative medium from agricultural by-products used for a starter of vinegar fermentation |
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