Determination of phenolic antioxidants in tuna fillets canned in hydrosols with HPLC‐DAD

Summary Canned tuna was fortified with a mixture of brine and hydrosols of aromatic plants (i.e. oregano, laurel, sage and lemon balm). An HPLC‐DAD method was developed and validated for the simultaneous determination of thirteen antioxidants in tuna fillets, including phenolic acids (gallic acid, v...

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Veröffentlicht in:International journal of food science & technology 2021-08, Vol.56 (8), p.4091-4097
Hauptverfasser: Kalogiouri, Natasa P., Kokokiris, Lambros E., Doulgeraki, Stephania, Papadopoulos, Athanasios, Samanidou, Victoria F.
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container_issue 8
container_start_page 4091
container_title International journal of food science & technology
container_volume 56
creator Kalogiouri, Natasa P.
Kokokiris, Lambros E.
Doulgeraki, Stephania
Papadopoulos, Athanasios
Samanidou, Victoria F.
description Summary Canned tuna was fortified with a mixture of brine and hydrosols of aromatic plants (i.e. oregano, laurel, sage and lemon balm). An HPLC‐DAD method was developed and validated for the simultaneous determination of thirteen antioxidants in tuna fillets, including phenolic acids (gallic acid, vanillic acid, syringic acid and rosmarinic acid), flavonoids (catechin, epicatechin, vanillin, myricetin, rutin, quercetin, luteolin and apigenin) and one hydroxybenzaldehyde (syringaldehyde). The analytes showed satisfying recovery efficiency (82.1–92.1%), and the method presented excellent linearity (r2 > 0.99). The precision limit was ≤5.6% RSDr for intra‐day and ≤7.2% RSDR for inter‐day experiments. The determined analytes ranged between 8.86 mg (quercetin) and 512 mg (rosmarinic acid) per 100g tuna flesh (n = 10), verifying that the hydrosols fortified the tuna fillets. Valorization of canned tuna fillets fortified with a mixture of brine and hydrosols of aromatic plants with the development of an HPLC‐DAD method for the determination of phenolic antioxidants.
doi_str_mv 10.1111/ijfs.15034
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An HPLC‐DAD method was developed and validated for the simultaneous determination of thirteen antioxidants in tuna fillets, including phenolic acids (gallic acid, vanillic acid, syringic acid and rosmarinic acid), flavonoids (catechin, epicatechin, vanillin, myricetin, rutin, quercetin, luteolin and apigenin) and one hydroxybenzaldehyde (syringaldehyde). The analytes showed satisfying recovery efficiency (82.1–92.1%), and the method presented excellent linearity (r2 &gt; 0.99). The precision limit was ≤5.6% RSDr for intra‐day and ≤7.2% RSDR for inter‐day experiments. The determined analytes ranged between 8.86 mg (quercetin) and 512 mg (rosmarinic acid) per 100g tuna flesh (n = 10), verifying that the hydrosols fortified the tuna fillets. 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An HPLC‐DAD method was developed and validated for the simultaneous determination of thirteen antioxidants in tuna fillets, including phenolic acids (gallic acid, vanillic acid, syringic acid and rosmarinic acid), flavonoids (catechin, epicatechin, vanillin, myricetin, rutin, quercetin, luteolin and apigenin) and one hydroxybenzaldehyde (syringaldehyde). The analytes showed satisfying recovery efficiency (82.1–92.1%), and the method presented excellent linearity (r2 &gt; 0.99). The precision limit was ≤5.6% RSDr for intra‐day and ≤7.2% RSDR for inter‐day experiments. The determined analytes ranged between 8.86 mg (quercetin) and 512 mg (rosmarinic acid) per 100g tuna flesh (n = 10), verifying that the hydrosols fortified the tuna fillets. 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technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kalogiouri, Natasa P.</au><au>Kokokiris, Lambros E.</au><au>Doulgeraki, Stephania</au><au>Papadopoulos, Athanasios</au><au>Samanidou, Victoria F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of phenolic antioxidants in tuna fillets canned in hydrosols with HPLC‐DAD</atitle><jtitle>International journal of food science &amp; technology</jtitle><date>2021-08</date><risdate>2021</risdate><volume>56</volume><issue>8</issue><spage>4091</spage><epage>4097</epage><pages>4091-4097</pages><issn>0950-5423</issn><eissn>1365-2621</eissn><abstract>Summary Canned tuna was fortified with a mixture of brine and hydrosols of aromatic plants (i.e. oregano, laurel, sage and lemon balm). An HPLC‐DAD method was developed and validated for the simultaneous determination of thirteen antioxidants in tuna fillets, including phenolic acids (gallic acid, vanillic acid, syringic acid and rosmarinic acid), flavonoids (catechin, epicatechin, vanillin, myricetin, rutin, quercetin, luteolin and apigenin) and one hydroxybenzaldehyde (syringaldehyde). The analytes showed satisfying recovery efficiency (82.1–92.1%), and the method presented excellent linearity (r2 &gt; 0.99). The precision limit was ≤5.6% RSDr for intra‐day and ≤7.2% RSDR for inter‐day experiments. The determined analytes ranged between 8.86 mg (quercetin) and 512 mg (rosmarinic acid) per 100g tuna flesh (n = 10), verifying that the hydrosols fortified the tuna fillets. 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source Wiley Online Library Journals Frontfile Complete; Oxford Journals Open Access Collection
subjects Acids
Analytical chemistry
Antioxidants
Catechin
Epicatechin
Fillets
Flavonoids
Fortification
functional
Gallic acid
High-performance liquid chromatography
HPLC
hydrosols
Hydroxybenzaldehydes
Liquid chromatography
Oregano
Phenolic acids
phenolic antioxidants
Phenols
Quercetin
Rosmarinic acid
Rutin
Syringaldehyde
Vanillic acid
Vanillin
title Determination of phenolic antioxidants in tuna fillets canned in hydrosols with HPLC‐DAD
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