Analysis of enzymatic extraction of saponin by experiment design methodology
Sapindus mukorossi (soapberry) is widely distributed in Taiwan, India, China and Japan. Its fruit contain saponin, which consist of two main part, the aglycone (sapogenin) and the polysaccharides (sugar moiety) which connected through glyosidic linkage. It has many pharmacological properties such as...
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| creator | Chen, I-C Fidela Chen, M-T Chung, T-W |
| description | Sapindus mukorossi (soapberry) is widely distributed in Taiwan, India, China and Japan. Its fruit contain saponin, which consist of two main part, the aglycone (sapogenin) and the polysaccharides (sugar moiety) which connected through glyosidic linkage. It has many pharmacological properties such as antimicrobial, anti-inflammatory, antioxidant, etc. Thus, this plant become one of high added value bioresources for pharmaceutical field. There are many methods to extract saponin. In this experiment, enzyme extraction is used to extract the saponin that known as high efficiency and specify method. Then, we analyzed its by-product, total polysaccharides content and its antioxidant properties through total polyphenol content. With Response Surface Methodology (RSM), we obtained the optimum condition for the best total saponin, total polysaccharides content, and total polyphenol content. Based on RSM analyses, with solid-liquid ratio of 1:5, extraction time of 3 hours, and enzyme concentration of 0.55%, we obtained the total saponin is 115.794 mg/mL, the total polysaccharides is 155.3 mg/mL and total polyphenol content is 1.309 mg/ml. |
| doi_str_mv | 10.1088/1755-1315/442/1/012005 |
| format | Article |
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Its fruit contain saponin, which consist of two main part, the aglycone (sapogenin) and the polysaccharides (sugar moiety) which connected through glyosidic linkage. It has many pharmacological properties such as antimicrobial, anti-inflammatory, antioxidant, etc. Thus, this plant become one of high added value bioresources for pharmaceutical field. There are many methods to extract saponin. In this experiment, enzyme extraction is used to extract the saponin that known as high efficiency and specify method. Then, we analyzed its by-product, total polysaccharides content and its antioxidant properties through total polyphenol content. With Response Surface Methodology (RSM), we obtained the optimum condition for the best total saponin, total polysaccharides content, and total polyphenol content. Based on RSM analyses, with solid-liquid ratio of 1:5, extraction time of 3 hours, and enzyme concentration of 0.55%, we obtained the total saponin is 115.794 mg/mL, the total polysaccharides is 155.3 mg/mL and total polyphenol content is 1.309 mg/ml.</description><identifier>ISSN: 1755-1307</identifier><identifier>EISSN: 1755-1315</identifier><identifier>DOI: 10.1088/1755-1315/442/1/012005</identifier><language>eng</language><publisher>Bristol: IOP Publishing</publisher><subject>Antiinfectives and antibacterials ; Antioxidants ; Design of experiments ; Enzymes ; Inflammation ; Polysaccharides ; Response surface methodology ; Saccharides ; Saponins</subject><ispartof>IOP conference series. Earth and environmental science, 2020-01, Vol.442 (1), p.12005</ispartof><rights>Published under licence by IOP Publishing Ltd</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). 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Earth and environmental science</title><addtitle>IOP Conf. Ser.: Earth Environ. Sci</addtitle><description>Sapindus mukorossi (soapberry) is widely distributed in Taiwan, India, China and Japan. Its fruit contain saponin, which consist of two main part, the aglycone (sapogenin) and the polysaccharides (sugar moiety) which connected through glyosidic linkage. It has many pharmacological properties such as antimicrobial, anti-inflammatory, antioxidant, etc. Thus, this plant become one of high added value bioresources for pharmaceutical field. There are many methods to extract saponin. In this experiment, enzyme extraction is used to extract the saponin that known as high efficiency and specify method. Then, we analyzed its by-product, total polysaccharides content and its antioxidant properties through total polyphenol content. With Response Surface Methodology (RSM), we obtained the optimum condition for the best total saponin, total polysaccharides content, and total polyphenol content. 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Earth and environmental science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, I-C</au><au>Fidela</au><au>Chen, M-T</au><au>Chung, T-W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of enzymatic extraction of saponin by experiment design methodology</atitle><jtitle>IOP conference series. Earth and environmental science</jtitle><addtitle>IOP Conf. Ser.: Earth Environ. Sci</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>442</volume><issue>1</issue><spage>12005</spage><pages>12005-</pages><issn>1755-1307</issn><eissn>1755-1315</eissn><abstract>Sapindus mukorossi (soapberry) is widely distributed in Taiwan, India, China and Japan. Its fruit contain saponin, which consist of two main part, the aglycone (sapogenin) and the polysaccharides (sugar moiety) which connected through glyosidic linkage. It has many pharmacological properties such as antimicrobial, anti-inflammatory, antioxidant, etc. Thus, this plant become one of high added value bioresources for pharmaceutical field. There are many methods to extract saponin. In this experiment, enzyme extraction is used to extract the saponin that known as high efficiency and specify method. Then, we analyzed its by-product, total polysaccharides content and its antioxidant properties through total polyphenol content. With Response Surface Methodology (RSM), we obtained the optimum condition for the best total saponin, total polysaccharides content, and total polyphenol content. Based on RSM analyses, with solid-liquid ratio of 1:5, extraction time of 3 hours, and enzyme concentration of 0.55%, we obtained the total saponin is 115.794 mg/mL, the total polysaccharides is 155.3 mg/mL and total polyphenol content is 1.309 mg/ml.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><doi>10.1088/1755-1315/442/1/012005</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antiinfectives and antibacterials Antioxidants Design of experiments Enzymes Inflammation Polysaccharides Response surface methodology Saccharides Saponins |
| title | Analysis of enzymatic extraction of saponin by experiment design methodology |
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