Isolation, characterization and genomic analysis of vB‐AhyM‐AP1, a lytic bacteriophage infecting Aeromonas hydrophila
Aims Aeromonas hydrophila is a zoonotic pathogen displaying resistance to multiple antibiotics. Here, we aim to develop a candidate biocontrol agent against A. hydrophila. Methods and Results In this study, we isolated and characterized the phage vB‐AhyM‐AP1 from sewage. It showed lytic activity aga...
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| Veröffentlicht in: | Journal of applied microbiology 2021-08, Vol.131 (2), p.695-705 |
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| creator | Pallavi, B. Puneeth, T.G. Shekar, M. Girisha, S.K. |
| description | Aims
Aeromonas hydrophila is a zoonotic pathogen displaying resistance to multiple antibiotics. Here, we aim to develop a candidate biocontrol agent against A. hydrophila.
Methods and Results
In this study, we isolated and characterized the phage vB‐AhyM‐AP1 from sewage. It showed lytic activity against A. hydrophila strains. One‐step growth curve revealed that the latent period lasted for 40 min. The burst size of one lytic cycle was 1413 PFU per infected cell. Temperature stability studies showed that the phage vB‐AhyM‐AP1 was active over temperatures ranging from 4 to 45°C for 1 h. pH stability studies indicated that the phage remained active within a pH range of 5–10 after 24 h of incubation. Stability tests in salt solutions showed that the phage was stable at salinities ranging from 0·1 to 2%. The phage also showed stabilities in organic solvents when incubated for 10 min. The Illumina Hiseq sequencing of its genome indicated that the phage vB‐AhyM‐AP1was a jumbo phage with a genome size of 2, 54 490 bp and GC content of 40·3%. The phylogenetic analysis of the terminase large subunit and major capsid protein indicated that the phage closely clustered with other Tevenvirinae phages. The genome encoded 455 ORFs and 22 tRNAs. The phage resulted in a reduction of 0·8 log units of viable A. hydrophila cells in biofilms grown on PVC coupons maintained in a low nutrient medium for 10 days.
Conclusions
The phage showed lytic activity against planktonic and biofilm cells of A. hydrophila. Genome‐based prediction showed it to be a strictly lytic phage without any virulence or antibiotic resistance genes indicating safety for environmental and clinical applications.
Significance and Impact of the Study
The multidrug‐resistant strains of A. hydrophila pose a significant health risk to both cultured fishes and consumers leaving few options for treatment. Phage vB‐AhyM‐AP1 may be used as a candidate biocontrol agent against A. hydrophila strains. |
| doi_str_mv | 10.1111/jam.14997 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2553467026</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2553467026</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3537-579b55f853b9b422ec3eb9a95aa250392f0a77629e52ec7a2469cc7f8599ef653</originalsourceid><addsrcrecordid>eNp1kE1OwzAQhS0EolBYcAFkiRUSKY4dx3hZKn7VChawjiau07pK4mKnoLDiCJyRk2Cawo7ZvNHMN0-ah9BRTAZxqPMFVIM4kVJsob2YpTyiqaDb6z6JOBG0h_a9XxASM8LTXdRjLKFEMLaH2jtvS2iMrc-wmoMD1Whn3tcTDPUUz3RtK6NCD2Xrjce2wK-XXx-fw3k7-ZHH-AwDLtsmQHl3bpdzmGls6kKrxtQzPNTOVrYGj-ft1IW1KeEA7RRQen240T56vr56Gt1G44ebu9FwHCnGmYi4kDnnxQVnucwTSrViOpcgOQDlhElaEBAipVLzsBNAk1QqJcKBlLpIOeujk8536ezLSvsmW9iVC9_4jHLOklQQmgbqtKOUs947XWRLZypwbRaT7CfkLIScrUMO7PHGcZVXevpH_qYagPMOeDOlbv93yu6Hk87yG5-BiHQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2553467026</pqid></control><display><type>article</type><title>Isolation, characterization and genomic analysis of vB‐AhyM‐AP1, a lytic bacteriophage infecting Aeromonas hydrophila</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Pallavi, B. ; Puneeth, T.G. ; Shekar, M. ; Girisha, S.K.</creator><creatorcontrib>Pallavi, B. ; Puneeth, T.G. ; Shekar, M. ; Girisha, S.K.</creatorcontrib><description>Aims
Aeromonas hydrophila is a zoonotic pathogen displaying resistance to multiple antibiotics. Here, we aim to develop a candidate biocontrol agent against A. hydrophila.
Methods and Results
In this study, we isolated and characterized the phage vB‐AhyM‐AP1 from sewage. It showed lytic activity against A. hydrophila strains. One‐step growth curve revealed that the latent period lasted for 40 min. The burst size of one lytic cycle was 1413 PFU per infected cell. Temperature stability studies showed that the phage vB‐AhyM‐AP1 was active over temperatures ranging from 4 to 45°C for 1 h. pH stability studies indicated that the phage remained active within a pH range of 5–10 after 24 h of incubation. Stability tests in salt solutions showed that the phage was stable at salinities ranging from 0·1 to 2%. The phage also showed stabilities in organic solvents when incubated for 10 min. The Illumina Hiseq sequencing of its genome indicated that the phage vB‐AhyM‐AP1was a jumbo phage with a genome size of 2, 54 490 bp and GC content of 40·3%. The phylogenetic analysis of the terminase large subunit and major capsid protein indicated that the phage closely clustered with other Tevenvirinae phages. The genome encoded 455 ORFs and 22 tRNAs. The phage resulted in a reduction of 0·8 log units of viable A. hydrophila cells in biofilms grown on PVC coupons maintained in a low nutrient medium for 10 days.
Conclusions
The phage showed lytic activity against planktonic and biofilm cells of A. hydrophila. Genome‐based prediction showed it to be a strictly lytic phage without any virulence or antibiotic resistance genes indicating safety for environmental and clinical applications.
Significance and Impact of the Study
The multidrug‐resistant strains of A. hydrophila pose a significant health risk to both cultured fishes and consumers leaving few options for treatment. Phage vB‐AhyM‐AP1 may be used as a candidate biocontrol agent against A. hydrophila strains.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14997</identifier><identifier>PMID: 33420733</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Aeromonas hydrophila ; Antibiotic resistance ; Antibiotics ; biocontrol ; biofilm ; Biofilms ; Biological control ; Burst size ; Capsid protein ; Gene sequencing ; genome ; Genomes ; Genomic analysis ; Health risks ; Latent period ; lytic phage ; Myoviridae ; Organic solvents ; pH effects ; Phages ; Phylogeny ; Saline solutions ; Sewage ; Stability tests ; Terminase ; Transcription factors ; Virulence</subject><ispartof>Journal of applied microbiology, 2021-08, Vol.131 (2), p.695-705</ispartof><rights>2021 The Society for Applied Microbiology</rights><rights>2021 The Society for Applied Microbiology.</rights><rights>Copyright © 2021 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-579b55f853b9b422ec3eb9a95aa250392f0a77629e52ec7a2469cc7f8599ef653</citedby><cites>FETCH-LOGICAL-c3537-579b55f853b9b422ec3eb9a95aa250392f0a77629e52ec7a2469cc7f8599ef653</cites><orcidid>0000-0003-0238-1954</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14997$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14997$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33420733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pallavi, B.</creatorcontrib><creatorcontrib>Puneeth, T.G.</creatorcontrib><creatorcontrib>Shekar, M.</creatorcontrib><creatorcontrib>Girisha, S.K.</creatorcontrib><title>Isolation, characterization and genomic analysis of vB‐AhyM‐AP1, a lytic bacteriophage infecting Aeromonas hydrophila</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
Aeromonas hydrophila is a zoonotic pathogen displaying resistance to multiple antibiotics. Here, we aim to develop a candidate biocontrol agent against A. hydrophila.
Methods and Results
In this study, we isolated and characterized the phage vB‐AhyM‐AP1 from sewage. It showed lytic activity against A. hydrophila strains. One‐step growth curve revealed that the latent period lasted for 40 min. The burst size of one lytic cycle was 1413 PFU per infected cell. Temperature stability studies showed that the phage vB‐AhyM‐AP1 was active over temperatures ranging from 4 to 45°C for 1 h. pH stability studies indicated that the phage remained active within a pH range of 5–10 after 24 h of incubation. Stability tests in salt solutions showed that the phage was stable at salinities ranging from 0·1 to 2%. The phage also showed stabilities in organic solvents when incubated for 10 min. The Illumina Hiseq sequencing of its genome indicated that the phage vB‐AhyM‐AP1was a jumbo phage with a genome size of 2, 54 490 bp and GC content of 40·3%. The phylogenetic analysis of the terminase large subunit and major capsid protein indicated that the phage closely clustered with other Tevenvirinae phages. The genome encoded 455 ORFs and 22 tRNAs. The phage resulted in a reduction of 0·8 log units of viable A. hydrophila cells in biofilms grown on PVC coupons maintained in a low nutrient medium for 10 days.
Conclusions
The phage showed lytic activity against planktonic and biofilm cells of A. hydrophila. Genome‐based prediction showed it to be a strictly lytic phage without any virulence or antibiotic resistance genes indicating safety for environmental and clinical applications.
Significance and Impact of the Study
The multidrug‐resistant strains of A. hydrophila pose a significant health risk to both cultured fishes and consumers leaving few options for treatment. Phage vB‐AhyM‐AP1 may be used as a candidate biocontrol agent against A. hydrophila strains.</description><subject>Aeromonas hydrophila</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>biocontrol</subject><subject>biofilm</subject><subject>Biofilms</subject><subject>Biological control</subject><subject>Burst size</subject><subject>Capsid protein</subject><subject>Gene sequencing</subject><subject>genome</subject><subject>Genomes</subject><subject>Genomic analysis</subject><subject>Health risks</subject><subject>Latent period</subject><subject>lytic phage</subject><subject>Myoviridae</subject><subject>Organic solvents</subject><subject>pH effects</subject><subject>Phages</subject><subject>Phylogeny</subject><subject>Saline solutions</subject><subject>Sewage</subject><subject>Stability tests</subject><subject>Terminase</subject><subject>Transcription factors</subject><subject>Virulence</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kE1OwzAQhS0EolBYcAFkiRUSKY4dx3hZKn7VChawjiau07pK4mKnoLDiCJyRk2Cawo7ZvNHMN0-ah9BRTAZxqPMFVIM4kVJsob2YpTyiqaDb6z6JOBG0h_a9XxASM8LTXdRjLKFEMLaH2jtvS2iMrc-wmoMD1Whn3tcTDPUUz3RtK6NCD2Xrjce2wK-XXx-fw3k7-ZHH-AwDLtsmQHl3bpdzmGls6kKrxtQzPNTOVrYGj-ft1IW1KeEA7RRQen240T56vr56Gt1G44ebu9FwHCnGmYi4kDnnxQVnucwTSrViOpcgOQDlhElaEBAipVLzsBNAk1QqJcKBlLpIOeujk8536ezLSvsmW9iVC9_4jHLOklQQmgbqtKOUs947XWRLZypwbRaT7CfkLIScrUMO7PHGcZVXevpH_qYagPMOeDOlbv93yu6Hk87yG5-BiHQ</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Pallavi, B.</creator><creator>Puneeth, T.G.</creator><creator>Shekar, M.</creator><creator>Girisha, S.K.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0003-0238-1954</orcidid></search><sort><creationdate>202108</creationdate><title>Isolation, characterization and genomic analysis of vB‐AhyM‐AP1, a lytic bacteriophage infecting Aeromonas hydrophila</title><author>Pallavi, B. ; Puneeth, T.G. ; Shekar, M. ; Girisha, S.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-579b55f853b9b422ec3eb9a95aa250392f0a77629e52ec7a2469cc7f8599ef653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aeromonas hydrophila</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>biocontrol</topic><topic>biofilm</topic><topic>Biofilms</topic><topic>Biological control</topic><topic>Burst size</topic><topic>Capsid protein</topic><topic>Gene sequencing</topic><topic>genome</topic><topic>Genomes</topic><topic>Genomic analysis</topic><topic>Health risks</topic><topic>Latent period</topic><topic>lytic phage</topic><topic>Myoviridae</topic><topic>Organic solvents</topic><topic>pH effects</topic><topic>Phages</topic><topic>Phylogeny</topic><topic>Saline solutions</topic><topic>Sewage</topic><topic>Stability tests</topic><topic>Terminase</topic><topic>Transcription factors</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pallavi, B.</creatorcontrib><creatorcontrib>Puneeth, T.G.</creatorcontrib><creatorcontrib>Shekar, M.</creatorcontrib><creatorcontrib>Girisha, S.K.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pallavi, B.</au><au>Puneeth, T.G.</au><au>Shekar, M.</au><au>Girisha, S.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation, characterization and genomic analysis of vB‐AhyM‐AP1, a lytic bacteriophage infecting Aeromonas hydrophila</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2021-08</date><risdate>2021</risdate><volume>131</volume><issue>2</issue><spage>695</spage><epage>705</epage><pages>695-705</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
Aeromonas hydrophila is a zoonotic pathogen displaying resistance to multiple antibiotics. Here, we aim to develop a candidate biocontrol agent against A. hydrophila.
Methods and Results
In this study, we isolated and characterized the phage vB‐AhyM‐AP1 from sewage. It showed lytic activity against A. hydrophila strains. One‐step growth curve revealed that the latent period lasted for 40 min. The burst size of one lytic cycle was 1413 PFU per infected cell. Temperature stability studies showed that the phage vB‐AhyM‐AP1 was active over temperatures ranging from 4 to 45°C for 1 h. pH stability studies indicated that the phage remained active within a pH range of 5–10 after 24 h of incubation. Stability tests in salt solutions showed that the phage was stable at salinities ranging from 0·1 to 2%. The phage also showed stabilities in organic solvents when incubated for 10 min. The Illumina Hiseq sequencing of its genome indicated that the phage vB‐AhyM‐AP1was a jumbo phage with a genome size of 2, 54 490 bp and GC content of 40·3%. The phylogenetic analysis of the terminase large subunit and major capsid protein indicated that the phage closely clustered with other Tevenvirinae phages. The genome encoded 455 ORFs and 22 tRNAs. The phage resulted in a reduction of 0·8 log units of viable A. hydrophila cells in biofilms grown on PVC coupons maintained in a low nutrient medium for 10 days.
Conclusions
The phage showed lytic activity against planktonic and biofilm cells of A. hydrophila. Genome‐based prediction showed it to be a strictly lytic phage without any virulence or antibiotic resistance genes indicating safety for environmental and clinical applications.
Significance and Impact of the Study
The multidrug‐resistant strains of A. hydrophila pose a significant health risk to both cultured fishes and consumers leaving few options for treatment. Phage vB‐AhyM‐AP1 may be used as a candidate biocontrol agent against A. hydrophila strains.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>33420733</pmid><doi>10.1111/jam.14997</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-0238-1954</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2021-08, Vol.131 (2), p.695-705 |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library Journals Frontfile Complete |
| subjects | Aeromonas hydrophila Antibiotic resistance Antibiotics biocontrol biofilm Biofilms Biological control Burst size Capsid protein Gene sequencing genome Genomes Genomic analysis Health risks Latent period lytic phage Myoviridae Organic solvents pH effects Phages Phylogeny Saline solutions Sewage Stability tests Terminase Transcription factors Virulence |
| title | Isolation, characterization and genomic analysis of vB‐AhyM‐AP1, a lytic bacteriophage infecting Aeromonas hydrophila |
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