Detection of chromosomal aberrations by fluorescence in situ hybridization in cervicovaginal biopsies from women exposed to diethylstilbestrol in utero
Epidemiologic studies have associated estrogens with human neoplasms such as those in the endometrium, cervix, vagina, breast, and liver. Perinatal exposure to natural (17β-estradiol [17β-E2]) and synthetic (diethylstilbestrol [DES]) estrogens induces neoplastic changes in humans and rodents. Previo...
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| Veröffentlicht in: | International journal of gynecological cancer 2006, Vol.16 (1), p.318-324 |
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| creator | Hajek, R. A. King, D. W. HernÁNdez-Valero, M. A. Kaufman, R. H. Liang, J. C. Chilton, J. A. Edwards, C. L. Wharton, J. T. Jones, L. A. |
| description | Epidemiologic studies have associated estrogens with human neoplasms such as those in the endometrium, cervix, vagina, breast, and liver. Perinatal exposure to natural (17β-estradiol [17β-E2]) and synthetic (diethylstilbestrol [DES]) estrogens induces neoplastic changes in humans and rodents. Previous studies demonstrated that neonatal 17β-E2 treatment of mice results in increased nuclear DNA content of cervicovaginal epithelium that precedes histologically evident neoplasia. In order to determine whether this effect was associated with chromosomal changes in humans, the frequencies of trisomy of chromosomes 1, 7, 11, and 17 were evaluated by the fluorescence in situ hybridization (FISH) technique in cervicovaginal tissue from 19 DES-exposed and 19 control women. The trisomic frequencies were significantly elevated in 4 of the 19 (21%) DES-exposed patients. One patient presented with trisomy of chromosomes 1, 7, and 11, while trisomy of chromosome 7 was observed in one patient. There were two patients with trisomy of chromosome 1. Trisomy of chromosomes 1, 7, 11, and 17 was not observed in the cervicovaginal tissue taken from control patients. These data suggest that DES-induced chromosomal trisomy may be an early event in the development of cervicovaginal neoplasia in humans. |
| doi_str_mv | 10.1136/ijgc-00009577-200601000-00051 |
| format | Article |
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A. ; King, D. W. ; HernÁNdez-Valero, M. A. ; Kaufman, R. H. ; Liang, J. C. ; Chilton, J. A. ; Edwards, C. L. ; Wharton, J. T. ; Jones, L. A.</creator><creatorcontrib>Hajek, R. A. ; King, D. W. ; HernÁNdez-Valero, M. A. ; Kaufman, R. H. ; Liang, J. C. ; Chilton, J. A. ; Edwards, C. L. ; Wharton, J. T. ; Jones, L. A.</creatorcontrib><description>Epidemiologic studies have associated estrogens with human neoplasms such as those in the endometrium, cervix, vagina, breast, and liver. Perinatal exposure to natural (17β-estradiol [17β-E2]) and synthetic (diethylstilbestrol [DES]) estrogens induces neoplastic changes in humans and rodents. Previous studies demonstrated that neonatal 17β-E2 treatment of mice results in increased nuclear DNA content of cervicovaginal epithelium that precedes histologically evident neoplasia. In order to determine whether this effect was associated with chromosomal changes in humans, the frequencies of trisomy of chromosomes 1, 7, 11, and 17 were evaluated by the fluorescence in situ hybridization (FISH) technique in cervicovaginal tissue from 19 DES-exposed and 19 control women. The trisomic frequencies were significantly elevated in 4 of the 19 (21%) DES-exposed patients. One patient presented with trisomy of chromosomes 1, 7, and 11, while trisomy of chromosome 7 was observed in one patient. There were two patients with trisomy of chromosome 1. Trisomy of chromosomes 1, 7, 11, and 17 was not observed in the cervicovaginal tissue taken from control patients. These data suggest that DES-induced chromosomal trisomy may be an early event in the development of cervicovaginal neoplasia in humans.</description><identifier>ISSN: 1048-891X</identifier><identifier>EISSN: 1525-1438</identifier><identifier>DOI: 10.1136/ijgc-00009577-200601000-00051</identifier><identifier>PMID: 16445652</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Adenocarcinoma, Clear Cell - chemically induced ; Adenocarcinoma, Clear Cell - epidemiology ; Adenocarcinoma, Clear Cell - pathology ; Adult ; Biopsy, Needle ; Case-Control Studies ; cervical ; Chromosome Aberrations - chemically induced ; Chromosome Aberrations - statistics & numerical data ; Chromosomes ; diethylstilbestrol ; Diethylstilbestrol - adverse effects ; Diethylstilbestrol - therapeutic use ; Estrogens ; Female ; fluorescence in situ hybridization (FISH) ; Humans ; In Situ Hybridization, Fluorescence ; Incidence ; Patients ; Probability ; Reference Values ; Risk Assessment ; Sensitivity and Specificity ; Tissue Culture Techniques ; Trisomy ; Uterine Cervical Neoplasms - chemically induced ; Uterine Cervical Neoplasms - epidemiology ; Uterine Cervical Neoplasms - pathology ; Uterus - drug effects ; Vaginal Neoplasms - chemically induced ; Vaginal Neoplasms - epidemiology ; Vaginal Neoplasms - pathology</subject><ispartof>International journal of gynecological cancer, 2006, Vol.16 (1), p.318-324</ispartof><rights>2006, IGCS</rights><rights>2006 IGCS and ESGO.</rights><rights>Copyright © 2006 Blackwell Publishing Ltd.</rights><rights>2006 2006, IGCS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-b2941-12510b580e066ede7250f6d075fb5d3fb746f2c57c1062ce7b30e7b6ffa9b583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16445652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hajek, R. A.</creatorcontrib><creatorcontrib>King, D. W.</creatorcontrib><creatorcontrib>HernÁNdez-Valero, M. A.</creatorcontrib><creatorcontrib>Kaufman, R. H.</creatorcontrib><creatorcontrib>Liang, J. C.</creatorcontrib><creatorcontrib>Chilton, J. A.</creatorcontrib><creatorcontrib>Edwards, C. L.</creatorcontrib><creatorcontrib>Wharton, J. T.</creatorcontrib><creatorcontrib>Jones, L. A.</creatorcontrib><title>Detection of chromosomal aberrations by fluorescence in situ hybridization in cervicovaginal biopsies from women exposed to diethylstilbestrol in utero</title><title>International journal of gynecological cancer</title><addtitle>Int J Gynecol Cancer</addtitle><description>Epidemiologic studies have associated estrogens with human neoplasms such as those in the endometrium, cervix, vagina, breast, and liver. Perinatal exposure to natural (17β-estradiol [17β-E2]) and synthetic (diethylstilbestrol [DES]) estrogens induces neoplastic changes in humans and rodents. Previous studies demonstrated that neonatal 17β-E2 treatment of mice results in increased nuclear DNA content of cervicovaginal epithelium that precedes histologically evident neoplasia. In order to determine whether this effect was associated with chromosomal changes in humans, the frequencies of trisomy of chromosomes 1, 7, 11, and 17 were evaluated by the fluorescence in situ hybridization (FISH) technique in cervicovaginal tissue from 19 DES-exposed and 19 control women. The trisomic frequencies were significantly elevated in 4 of the 19 (21%) DES-exposed patients. One patient presented with trisomy of chromosomes 1, 7, and 11, while trisomy of chromosome 7 was observed in one patient. There were two patients with trisomy of chromosome 1. Trisomy of chromosomes 1, 7, 11, and 17 was not observed in the cervicovaginal tissue taken from control patients. These data suggest that DES-induced chromosomal trisomy may be an early event in the development of cervicovaginal neoplasia in humans.</description><subject>Adenocarcinoma, Clear Cell - chemically induced</subject><subject>Adenocarcinoma, Clear Cell - epidemiology</subject><subject>Adenocarcinoma, Clear Cell - pathology</subject><subject>Adult</subject><subject>Biopsy, Needle</subject><subject>Case-Control Studies</subject><subject>cervical</subject><subject>Chromosome Aberrations - chemically induced</subject><subject>Chromosome Aberrations - statistics & numerical data</subject><subject>Chromosomes</subject><subject>diethylstilbestrol</subject><subject>Diethylstilbestrol - adverse effects</subject><subject>Diethylstilbestrol - therapeutic use</subject><subject>Estrogens</subject><subject>Female</subject><subject>fluorescence in situ hybridization (FISH)</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Incidence</subject><subject>Patients</subject><subject>Probability</subject><subject>Reference Values</subject><subject>Risk Assessment</subject><subject>Sensitivity and Specificity</subject><subject>Tissue Culture Techniques</subject><subject>Trisomy</subject><subject>Uterine Cervical Neoplasms - chemically induced</subject><subject>Uterine Cervical Neoplasms - epidemiology</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Uterus - drug effects</subject><subject>Vaginal Neoplasms - chemically induced</subject><subject>Vaginal Neoplasms - epidemiology</subject><subject>Vaginal Neoplasms - pathology</subject><issn>1048-891X</issn><issn>1525-1438</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqVkc1u1DAUhSNERUvhFZAlxDJgO7GTLFigFgpSpW66YGfF9nXjwYkH25lh-iJ9XZxmgBUSeOHfc46v_RXFG4LfElLxd3Zzp0qcW8eapqQYc0zyatli5ElxRhhlJamr9mme47ot2458PS2ex7hZTBR3z4pTwuuacUbPiodLSKCS9RPyBqkh-NFHP_YO9RJC6JeTiOQBGTf7AFHBpADZCUWbZjQcZLDa3j_Kll0FYWeV3_V3dsoZ0vpttBCRyblo70eYEPzY-ggaJY-0hTQcXEzWSYgpeLdkzAmCf1GcmN5FeHkcz4vbTx9vLz6X1zdXXy4-XJeSdjUpCWUES9ZiwJyDhoYybLjGDTOS6crIpuaGKtYogjlV0MgK544b03fZVp0Xr9fYbfDf51yD2Pg55NKjoIzRlrRd22XV-1Wlgo8xgBHbYMc-HATBYqEiFiriFxXxm4p4pJL9r463zHIE_cd9xJAF9SrYe5dfH7-5eQ9BDNC7NIi_0c62q9UG-Yd2NjuisgsgbUOGKrS3_1xhuybJcfOfj_sJBgbD5w</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Hajek, R. 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A.</creator><general>Elsevier Inc</general><general>Copyright Blackwell Publishing Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>2006</creationdate><title>Detection of chromosomal aberrations by fluorescence in situ hybridization in cervicovaginal biopsies from women exposed to diethylstilbestrol in utero</title><author>Hajek, R. A. ; King, D. W. ; HernÁNdez-Valero, M. A. ; Kaufman, R. H. ; Liang, J. C. ; Chilton, J. A. ; Edwards, C. L. ; Wharton, J. T. ; Jones, L. 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A.</au><au>King, D. W.</au><au>HernÁNdez-Valero, M. A.</au><au>Kaufman, R. H.</au><au>Liang, J. C.</au><au>Chilton, J. A.</au><au>Edwards, C. L.</au><au>Wharton, J. T.</au><au>Jones, L. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of chromosomal aberrations by fluorescence in situ hybridization in cervicovaginal biopsies from women exposed to diethylstilbestrol in utero</atitle><jtitle>International journal of gynecological cancer</jtitle><addtitle>Int J Gynecol Cancer</addtitle><date>2006</date><risdate>2006</risdate><volume>16</volume><issue>1</issue><spage>318</spage><epage>324</epage><pages>318-324</pages><issn>1048-891X</issn><eissn>1525-1438</eissn><abstract>Epidemiologic studies have associated estrogens with human neoplasms such as those in the endometrium, cervix, vagina, breast, and liver. Perinatal exposure to natural (17β-estradiol [17β-E2]) and synthetic (diethylstilbestrol [DES]) estrogens induces neoplastic changes in humans and rodents. Previous studies demonstrated that neonatal 17β-E2 treatment of mice results in increased nuclear DNA content of cervicovaginal epithelium that precedes histologically evident neoplasia. In order to determine whether this effect was associated with chromosomal changes in humans, the frequencies of trisomy of chromosomes 1, 7, 11, and 17 were evaluated by the fluorescence in situ hybridization (FISH) technique in cervicovaginal tissue from 19 DES-exposed and 19 control women. The trisomic frequencies were significantly elevated in 4 of the 19 (21%) DES-exposed patients. One patient presented with trisomy of chromosomes 1, 7, and 11, while trisomy of chromosome 7 was observed in one patient. There were two patients with trisomy of chromosome 1. Trisomy of chromosomes 1, 7, 11, and 17 was not observed in the cervicovaginal tissue taken from control patients. These data suggest that DES-induced chromosomal trisomy may be an early event in the development of cervicovaginal neoplasia in humans.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>16445652</pmid><doi>10.1136/ijgc-00009577-200601000-00051</doi><tpages>7</tpages></addata></record> |
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| ispartof | International journal of gynecological cancer, 2006, Vol.16 (1), p.318-324 |
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| subjects | Adenocarcinoma, Clear Cell - chemically induced Adenocarcinoma, Clear Cell - epidemiology Adenocarcinoma, Clear Cell - pathology Adult Biopsy, Needle Case-Control Studies cervical Chromosome Aberrations - chemically induced Chromosome Aberrations - statistics & numerical data Chromosomes diethylstilbestrol Diethylstilbestrol - adverse effects Diethylstilbestrol - therapeutic use Estrogens Female fluorescence in situ hybridization (FISH) Humans In Situ Hybridization, Fluorescence Incidence Patients Probability Reference Values Risk Assessment Sensitivity and Specificity Tissue Culture Techniques Trisomy Uterine Cervical Neoplasms - chemically induced Uterine Cervical Neoplasms - epidemiology Uterine Cervical Neoplasms - pathology Uterus - drug effects Vaginal Neoplasms - chemically induced Vaginal Neoplasms - epidemiology Vaginal Neoplasms - pathology |
| title | Detection of chromosomal aberrations by fluorescence in situ hybridization in cervicovaginal biopsies from women exposed to diethylstilbestrol in utero |
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