Simultaneous detection of 10 viruses in globe artichoke by a synthetic oligonucleotide-based DNA polyprobe
Most of the 26 viruses that infect globe artichoke are detrimental to the crop’s production, quality, and nutritional value, hamper the development of a nursery activity in the respect of current EU legislation and pose risks for the new plantings. Worldwide movement of virus-controlled plant propag...
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| Veröffentlicht in: | European journal of plant pathology 2021-08, Vol.160 (4), p.991-997 |
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| creator | Minutillo, Serena Anna Spanò, Roberta Gallitelli, Donato Mascia, Tiziana |
| description | Most of the 26 viruses that infect globe artichoke are detrimental to the crop’s production, quality, and nutritional value, hamper the development of a nursery activity in the respect of current EU legislation and pose risks for the new plantings. Worldwide movement of virus-controlled plant propagative material require fast, accurate and feasible methods to guarantee reliable results and detection of multiple viruses in a single assay to reduce time and costs in routine tests. Here we describe the use of a digoxigenin-labelled polyprobe assembled from synthetic 50 nt single-stranded DNA sequences derived from genomes of the most commonly occurring viruses in globe artichoke. These included artichoke Italian latent virus (AILV), artichoke latent virus (ArLV), artichoke mottled crinkle virus (AMCV), turnip mosaic virus (TuMV), tomato infectious chlorosis virus (TICV), bean yellow mosaic virus (BYMV), cucumber mosaic virus (CMV), pelargonium zonate spot virus (PZSV), tomato spotted wilt virus (TSWV) and tobacco mosaic virus (TMV). The polyprobe described herein represent the first cheap and reliable example of a technology for broad-spectrum surveys in globe artichoke. |
| doi_str_mv | 10.1007/s10658-021-02292-x |
| format | Article |
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Worldwide movement of virus-controlled plant propagative material require fast, accurate and feasible methods to guarantee reliable results and detection of multiple viruses in a single assay to reduce time and costs in routine tests. Here we describe the use of a digoxigenin-labelled polyprobe assembled from synthetic 50 nt single-stranded DNA sequences derived from genomes of the most commonly occurring viruses in globe artichoke. These included artichoke Italian latent virus (AILV), artichoke latent virus (ArLV), artichoke mottled crinkle virus (AMCV), turnip mosaic virus (TuMV), tomato infectious chlorosis virus (TICV), bean yellow mosaic virus (BYMV), cucumber mosaic virus (CMV), pelargonium zonate spot virus (PZSV), tomato spotted wilt virus (TSWV) and tobacco mosaic virus (TMV). 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Worldwide movement of virus-controlled plant propagative material require fast, accurate and feasible methods to guarantee reliable results and detection of multiple viruses in a single assay to reduce time and costs in routine tests. Here we describe the use of a digoxigenin-labelled polyprobe assembled from synthetic 50 nt single-stranded DNA sequences derived from genomes of the most commonly occurring viruses in globe artichoke. These included artichoke Italian latent virus (AILV), artichoke latent virus (ArLV), artichoke mottled crinkle virus (AMCV), turnip mosaic virus (TuMV), tomato infectious chlorosis virus (TICV), bean yellow mosaic virus (BYMV), cucumber mosaic virus (CMV), pelargonium zonate spot virus (PZSV), tomato spotted wilt virus (TSWV) and tobacco mosaic virus (TMV). The polyprobe described herein represent the first cheap and reliable example of a technology for broad-spectrum surveys in globe artichoke.</description><subject>Agriculture</subject><subject>Artichokes</subject><subject>Biomedical and Life Sciences</subject><subject>Chlorosis</subject><subject>Crop production</subject><subject>Cynara scolymus</subject><subject>Deoxyribonucleic acid</subject><subject>Digoxigenin</subject><subject>DNA</subject><subject>Ecology</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Legislation</subject><subject>Life Sciences</subject><subject>Nucleotide sequence</subject><subject>Nutritive value</subject><subject>Oligonucleotides</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Single-stranded DNA</subject><subject>Tobacco</subject><subject>Tomatoes</subject><subject>Viruses</subject><subject>Wilt</subject><issn>0929-1873</issn><issn>1573-8469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1LxDAQhoMouK7-AU8Bz9UkTdrmuPgNix7Uc0ja6W7WbrMmqWz_vdEK3jwMA8P7vAMPQueUXFJCyqtASSGqjDCahkmW7Q_QjIoyzypeyEM0I5LJjFZlfoxOQtiQBEnJZmjzYrdDF3UPbgi4gQh1tK7HrsWU4E_rhwAB2x6vOmcAax9tvXbvgM2INQ5jH9eQTth1duX6oe7ARdtAZnSABt88LfDOdePOJ_gUHbW6C3D2u-fo7e729fohWz7fP14vllmdUxkzDbytK8ipaAlljSlLQok2AlooTMNF0WottBSMiopJYXTJjaQVNIzTkkiZz9HF1Ju-fgwQotq4wffppWJCEMk5ZyKl2JSqvQvBQ6t23m61HxUl6tupmpyq5FT9OFX7BOUTFFK4X4H_q_6H-gLoMXti</recordid><startdate>20210801</startdate><enddate>20210801</enddate><creator>Minutillo, Serena Anna</creator><creator>Spanò, Roberta</creator><creator>Gallitelli, Donato</creator><creator>Mascia, Tiziana</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20210801</creationdate><title>Simultaneous detection of 10 viruses in globe artichoke by a synthetic oligonucleotide-based DNA polyprobe</title><author>Minutillo, Serena Anna ; Spanò, Roberta ; Gallitelli, Donato ; Mascia, Tiziana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-ae4fc8e315f012db77010ab5efe6bd456faa5a952158295ba74b918ed24170993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agriculture</topic><topic>Artichokes</topic><topic>Biomedical and Life Sciences</topic><topic>Chlorosis</topic><topic>Crop production</topic><topic>Cynara scolymus</topic><topic>Deoxyribonucleic acid</topic><topic>Digoxigenin</topic><topic>DNA</topic><topic>Ecology</topic><topic>Gene sequencing</topic><topic>Genomes</topic><topic>Legislation</topic><topic>Life Sciences</topic><topic>Nucleotide sequence</topic><topic>Nutritive value</topic><topic>Oligonucleotides</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Single-stranded DNA</topic><topic>Tobacco</topic><topic>Tomatoes</topic><topic>Viruses</topic><topic>Wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minutillo, Serena Anna</creatorcontrib><creatorcontrib>Spanò, Roberta</creatorcontrib><creatorcontrib>Gallitelli, Donato</creatorcontrib><creatorcontrib>Mascia, Tiziana</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>European journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Minutillo, Serena Anna</au><au>Spanò, Roberta</au><au>Gallitelli, Donato</au><au>Mascia, Tiziana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous detection of 10 viruses in globe artichoke by a synthetic oligonucleotide-based DNA polyprobe</atitle><jtitle>European journal of plant pathology</jtitle><stitle>Eur J Plant Pathol</stitle><date>2021-08-01</date><risdate>2021</risdate><volume>160</volume><issue>4</issue><spage>991</spage><epage>997</epage><pages>991-997</pages><issn>0929-1873</issn><eissn>1573-8469</eissn><abstract>Most of the 26 viruses that infect globe artichoke are detrimental to the crop’s production, quality, and nutritional value, hamper the development of a nursery activity in the respect of current EU legislation and pose risks for the new plantings. Worldwide movement of virus-controlled plant propagative material require fast, accurate and feasible methods to guarantee reliable results and detection of multiple viruses in a single assay to reduce time and costs in routine tests. Here we describe the use of a digoxigenin-labelled polyprobe assembled from synthetic 50 nt single-stranded DNA sequences derived from genomes of the most commonly occurring viruses in globe artichoke. These included artichoke Italian latent virus (AILV), artichoke latent virus (ArLV), artichoke mottled crinkle virus (AMCV), turnip mosaic virus (TuMV), tomato infectious chlorosis virus (TICV), bean yellow mosaic virus (BYMV), cucumber mosaic virus (CMV), pelargonium zonate spot virus (PZSV), tomato spotted wilt virus (TSWV) and tobacco mosaic virus (TMV). The polyprobe described herein represent the first cheap and reliable example of a technology for broad-spectrum surveys in globe artichoke.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10658-021-02292-x</doi><tpages>7</tpages></addata></record> |
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| subjects | Agriculture Artichokes Biomedical and Life Sciences Chlorosis Crop production Cynara scolymus Deoxyribonucleic acid Digoxigenin DNA Ecology Gene sequencing Genomes Legislation Life Sciences Nucleotide sequence Nutritive value Oligonucleotides Plant Pathology Plant Sciences Single-stranded DNA Tobacco Tomatoes Viruses Wilt |
| title | Simultaneous detection of 10 viruses in globe artichoke by a synthetic oligonucleotide-based DNA polyprobe |
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