Lipid Isolation Process and Study on Some Molecular Species of Polar Lipid Isolated from Seed of Madhuca ellitica
This study attempted the lipid extraction process from the seeds of Madhuca ellitica, a lipid-rich plant, and conducted a lipidomic analysis on molecular species of the obtained product. Total lipids of the crude seeds were found to contain 11.2% of polar lipids. The major fatty acids (FAs) of the p...
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| Veröffentlicht in: | Processes 2019-06, Vol.7 (6), p.375 |
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| creator | Phuong, Doan Lan Toan, Tran Quoc Dang, Ly P. T. Imbs, Andrey B. Long, Pham Quoc Thang, Tran Dinh Matthaeus, Bertrand Bach, Long Giang Bui, Le Minh |
| description | This study attempted the lipid extraction process from the seeds of Madhuca ellitica, a lipid-rich plant, and conducted a lipidomic analysis on molecular species of the obtained product. Total lipids of the crude seeds were found to contain 11.2% of polar lipids. The major fatty acids (FAs) of the polar lipids were palmitic (16:0), stearic (18:0), oleic (18:1n-9), and linoleic (18:2n-6) acids, which amounted to 28.5, 12.5, 44.8, and 13.2% of total FAs, respectively. The content and chemical structures of individual molecular species of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid (PA), and sulfoquinovosyldiacylglycerol (SQDG) were determined by HPLC with a tandem high-resolution mass spectrometry (HRMS). The major molecular species were 18:1/18:2 PE, 16:0/18:1 PC, 18:1/18:2 PC, 16:0/18:2 PG, 16:0/18:1 PG, 16:1/18:1 PI, 16:0/18:1 PI, 18:0/18:2 PI, 16:0/18:1 PA, 18:1/18:2 PA, 16:0/18:1 SQDG, and 18:0/18:1 SQDG. The application of a tandem HRMS allows us to determine the content of each isomer in pairs of the monoisotopic molecular species, for example, 18:0/18:2 and 18:1/18:1. The evaluation of the seed polar lipid profile will be helpful for developing the potential of this tree for nutritive and industrial uses. |
| doi_str_mv | 10.3390/pr7060375 |
| format | Article |
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The content and chemical structures of individual molecular species of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid (PA), and sulfoquinovosyldiacylglycerol (SQDG) were determined by HPLC with a tandem high-resolution mass spectrometry (HRMS). The major molecular species were 18:1/18:2 PE, 16:0/18:1 PC, 18:1/18:2 PC, 16:0/18:2 PG, 16:0/18:1 PG, 16:1/18:1 PI, 16:0/18:1 PI, 18:0/18:2 PI, 16:0/18:1 PA, 18:1/18:2 PA, 16:0/18:1 SQDG, and 18:0/18:1 SQDG. The application of a tandem HRMS allows us to determine the content of each isomer in pairs of the monoisotopic molecular species, for example, 18:0/18:2 and 18:1/18:1. The evaluation of the seed polar lipid profile will be helpful for developing the potential of this tree for nutritive and industrial uses.</description><identifier>ISSN: 2227-9717</identifier><identifier>EISSN: 2227-9717</identifier><identifier>DOI: 10.3390/pr7060375</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Chromatography ; Fatty acids ; High-performance liquid chromatography ; Industrial applications ; Lecithin ; Lipids ; Madhuca ; Mass spectrometry ; Mass spectroscopy ; Phosphatidic acid ; Phosphatidylcholine ; Phosphatidylethanolamine ; Phosphatidylglycerol ; Phosphatidylinositol ; Scientific imaging ; Seeds ; Sensors ; Species</subject><ispartof>Processes, 2019-06, Vol.7 (6), p.375</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). 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The content and chemical structures of individual molecular species of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid (PA), and sulfoquinovosyldiacylglycerol (SQDG) were determined by HPLC with a tandem high-resolution mass spectrometry (HRMS). The major molecular species were 18:1/18:2 PE, 16:0/18:1 PC, 18:1/18:2 PC, 16:0/18:2 PG, 16:0/18:1 PG, 16:1/18:1 PI, 16:0/18:1 PI, 18:0/18:2 PI, 16:0/18:1 PA, 18:1/18:2 PA, 16:0/18:1 SQDG, and 18:0/18:1 SQDG. The application of a tandem HRMS allows us to determine the content of each isomer in pairs of the monoisotopic molecular species, for example, 18:0/18:2 and 18:1/18:1. The evaluation of the seed polar lipid profile will be helpful for developing the potential of this tree for nutritive and industrial uses.</description><subject>Chromatography</subject><subject>Fatty acids</subject><subject>High-performance liquid chromatography</subject><subject>Industrial applications</subject><subject>Lecithin</subject><subject>Lipids</subject><subject>Madhuca</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Phosphatidic acid</subject><subject>Phosphatidylcholine</subject><subject>Phosphatidylethanolamine</subject><subject>Phosphatidylglycerol</subject><subject>Phosphatidylinositol</subject><subject>Scientific imaging</subject><subject>Seeds</subject><subject>Sensors</subject><subject>Species</subject><issn>2227-9717</issn><issn>2227-9717</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpNkEtrwzAQhEVpoSHNof9A0FMPbvWwLPtYQh8Bhwbcno0eK-rgRI5kH_Lvq5BSspcdho9ZdhC6p-SJ84o8D0GSgnAprtCMMSazSlJ5faFv0SLGLUlTUV6KYoYOdTd0Fq-i79XY-T3eBG8gRqz2FjfjZI84mY3fAV77HszUq4CbAUwHEXuHN_5kXIaAxS74HW4gqUSslf2ZjMLQ993YGXWHbpzqIyz-9hx9v71-LT-y-vN9tXypM8MqNmaFKgUHXVpJtdFC5ZZrmmtNSqmhUIXVOaPOSVYCCGWNJASYFMyl93NuLJ-jh3PuEPxhgji2Wz-FfTrZMiFIqoRXZaIez5QJPsYArh1Ct1Ph2FLSnkpt_0vlv4i3ac8</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Phuong, Doan Lan</creator><creator>Toan, Tran Quoc</creator><creator>Dang, Ly P. 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T. ; Imbs, Andrey B. ; Long, Pham Quoc ; Thang, Tran Dinh ; Matthaeus, Bertrand ; Bach, Long Giang ; Bui, Le Minh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c292t-6a853eb8d71bcb5a4d3b14bb087be6a6db421ff728ee5adc700e2752f37543cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Chromatography</topic><topic>Fatty acids</topic><topic>High-performance liquid chromatography</topic><topic>Industrial applications</topic><topic>Lecithin</topic><topic>Lipids</topic><topic>Madhuca</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Phosphatidic acid</topic><topic>Phosphatidylcholine</topic><topic>Phosphatidylethanolamine</topic><topic>Phosphatidylglycerol</topic><topic>Phosphatidylinositol</topic><topic>Scientific imaging</topic><topic>Seeds</topic><topic>Sensors</topic><topic>Species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phuong, Doan Lan</creatorcontrib><creatorcontrib>Toan, Tran Quoc</creatorcontrib><creatorcontrib>Dang, Ly P. 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T.</au><au>Imbs, Andrey B.</au><au>Long, Pham Quoc</au><au>Thang, Tran Dinh</au><au>Matthaeus, Bertrand</au><au>Bach, Long Giang</au><au>Bui, Le Minh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lipid Isolation Process and Study on Some Molecular Species of Polar Lipid Isolated from Seed of Madhuca ellitica</atitle><jtitle>Processes</jtitle><date>2019-06-01</date><risdate>2019</risdate><volume>7</volume><issue>6</issue><spage>375</spage><pages>375-</pages><issn>2227-9717</issn><eissn>2227-9717</eissn><abstract>This study attempted the lipid extraction process from the seeds of Madhuca ellitica, a lipid-rich plant, and conducted a lipidomic analysis on molecular species of the obtained product. Total lipids of the crude seeds were found to contain 11.2% of polar lipids. The major fatty acids (FAs) of the polar lipids were palmitic (16:0), stearic (18:0), oleic (18:1n-9), and linoleic (18:2n-6) acids, which amounted to 28.5, 12.5, 44.8, and 13.2% of total FAs, respectively. The content and chemical structures of individual molecular species of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid (PA), and sulfoquinovosyldiacylglycerol (SQDG) were determined by HPLC with a tandem high-resolution mass spectrometry (HRMS). The major molecular species were 18:1/18:2 PE, 16:0/18:1 PC, 18:1/18:2 PC, 16:0/18:2 PG, 16:0/18:1 PG, 16:1/18:1 PI, 16:0/18:1 PI, 18:0/18:2 PI, 16:0/18:1 PA, 18:1/18:2 PA, 16:0/18:1 SQDG, and 18:0/18:1 SQDG. The application of a tandem HRMS allows us to determine the content of each isomer in pairs of the monoisotopic molecular species, for example, 18:0/18:2 and 18:1/18:1. The evaluation of the seed polar lipid profile will be helpful for developing the potential of this tree for nutritive and industrial uses.</abstract><cop>Basel</cop><pub>MDPI AG</pub><doi>10.3390/pr7060375</doi><orcidid>https://orcid.org/0000-0002-1283-8324</orcidid><orcidid>https://orcid.org/0000-0002-0537-0903</orcidid><orcidid>https://orcid.org/0000-0003-0760-5750</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Chromatography Fatty acids High-performance liquid chromatography Industrial applications Lecithin Lipids Madhuca Mass spectrometry Mass spectroscopy Phosphatidic acid Phosphatidylcholine Phosphatidylethanolamine Phosphatidylglycerol Phosphatidylinositol Scientific imaging Seeds Sensors Species |
| title | Lipid Isolation Process and Study on Some Molecular Species of Polar Lipid Isolated from Seed of Madhuca ellitica |
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