The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti
Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates that positively or negatively modify amplification in the invertebrate host. We used deep sequencing to compare the differential transcript abundances in Aedes aegypti 14 days post den...
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| Veröffentlicht in: | International journal of molecular sciences 2020-10, Vol.21 (20), p.7520, Article 7520 |
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| creator | Runtuwene, Lucky R. Kawashima, Shuichi Pijoh, Victor D. Tuda, Josef S. B. Hayashida, Kyoko Yamagishi, Junya Sugimoto, Chihiro Nishiyama, Shoko Sasaki, Michihito Orba, Yasuko Sawa, Hirofumi Takasaki, Tomohiko James, Anthony A. Kobayashi, Takashi Eshita, Yuki |
| description | Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates that positively or negatively modify amplification in the invertebrate host. We used deep sequencing to compare the differential transcript abundances in Aedes aegypti 14 days post dengue infection to those of uninfected A. aegypti. The gene lethal(2)-essential-for-life [l(2)efl], which encodes a member of the heat shock 20 protein (HSP20) family, was upregulated following dengue virus type 2 (DENV-2) infection in vivo. The transcripts of this gene did not exhibit differential accumulation in mosquitoes exposed to insecticides or pollutants. The induction and overexpression of l(2)efl gene products using poly(I:C) resulted in decreased DENV-2 replication in the cell line. In contrast, the RNAi-mediated suppression of l(2)efl gene products resulted in enhanced DENV-2 replication, but this enhancement occurred only if multiple l(2)efl genes were suppressed. l(2)efl homologs induce the phosphorylation of eukaryotic initiation factor 2 alpha (eIF2 alpha) in the fruit fly Drosophila melanogaster, and we confirmed this finding in the cell line. However, the mechanism by which l(2)efl phosphorylates eIF2 alpha remains unclear. We conclude that l(2)efl encodes a potential anti-dengue protein in the vector mosquito. |
| doi_str_mv | 10.3390/ijms21207520 |
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B. ; Hayashida, Kyoko ; Yamagishi, Junya ; Sugimoto, Chihiro ; Nishiyama, Shoko ; Sasaki, Michihito ; Orba, Yasuko ; Sawa, Hirofumi ; Takasaki, Tomohiko ; James, Anthony A. ; Kobayashi, Takashi ; Eshita, Yuki</creator><creatorcontrib>Runtuwene, Lucky R. ; Kawashima, Shuichi ; Pijoh, Victor D. ; Tuda, Josef S. B. ; Hayashida, Kyoko ; Yamagishi, Junya ; Sugimoto, Chihiro ; Nishiyama, Shoko ; Sasaki, Michihito ; Orba, Yasuko ; Sawa, Hirofumi ; Takasaki, Tomohiko ; James, Anthony A. ; Kobayashi, Takashi ; Eshita, Yuki</creatorcontrib><description>Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates that positively or negatively modify amplification in the invertebrate host. We used deep sequencing to compare the differential transcript abundances in Aedes aegypti 14 days post dengue infection to those of uninfected A. aegypti. The gene lethal(2)-essential-for-life [l(2)efl], which encodes a member of the heat shock 20 protein (HSP20) family, was upregulated following dengue virus type 2 (DENV-2) infection in vivo. The transcripts of this gene did not exhibit differential accumulation in mosquitoes exposed to insecticides or pollutants. The induction and overexpression of l(2)efl gene products using poly(I:C) resulted in decreased DENV-2 replication in the cell line. In contrast, the RNAi-mediated suppression of l(2)efl gene products resulted in enhanced DENV-2 replication, but this enhancement occurred only if multiple l(2)efl genes were suppressed. l(2)efl homologs induce the phosphorylation of eukaryotic initiation factor 2 alpha (eIF2 alpha) in the fruit fly Drosophila melanogaster, and we confirmed this finding in the cell line. However, the mechanism by which l(2)efl phosphorylates eIF2 alpha remains unclear. 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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). 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B.</creatorcontrib><creatorcontrib>Hayashida, Kyoko</creatorcontrib><creatorcontrib>Yamagishi, Junya</creatorcontrib><creatorcontrib>Sugimoto, Chihiro</creatorcontrib><creatorcontrib>Nishiyama, Shoko</creatorcontrib><creatorcontrib>Sasaki, Michihito</creatorcontrib><creatorcontrib>Orba, Yasuko</creatorcontrib><creatorcontrib>Sawa, Hirofumi</creatorcontrib><creatorcontrib>Takasaki, Tomohiko</creatorcontrib><creatorcontrib>James, Anthony A.</creatorcontrib><creatorcontrib>Kobayashi, Takashi</creatorcontrib><creatorcontrib>Eshita, Yuki</creatorcontrib><title>The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti</title><title>International journal of molecular sciences</title><addtitle>INT J MOL SCI</addtitle><addtitle>Int J Mol Sci</addtitle><description>Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates that positively or negatively modify amplification in the invertebrate host. 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We conclude that l(2)efl encodes a potential anti-dengue protein in the vector mosquito.</description><subject>Aedes - genetics</subject><subject>Aedes - virology</subject><subject>Aedes aegypti</subject><subject>Animals</subject><subject>Biochemistry & Molecular Biology</subject><subject>Chemistry</subject><subject>Chemistry, Multidisciplinary</subject><subject>Computational Biology - methods</subject><subject>dengue</subject><subject>Dengue - virology</subject><subject>Dengue fever</subject><subject>Dengue Virus - physiology</subject><subject>Exocrine glands</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Genes</subject><subject>Genomes</subject><subject>Heat shock</subject><subject>Heat shock proteins</subject><subject>Homology</subject><subject>Host-Pathogen Interactions</subject><subject>HSP20 Heat-Shock Proteins - genetics</subject><subject>Immunocompetence</subject><subject>Infections</subject><subject>Initiation factor eIF-2α</subject><subject>Insect Proteins - genetics</subject><subject>Insecticides</subject><subject>Invertebrates</subject><subject>lethal-essential-for-life</subject><subject>Life Sciences & Biomedicine</subject><subject>Mosquito Vectors - genetics</subject><subject>Mosquito Vectors - virology</subject><subject>Mosquitoes</subject><subject>Phosphorylation</subject><subject>Physical Sciences</subject><subject>Pollutants</subject><subject>Polyinosinic:polycytidylic acid</subject><subject>Replication</subject><subject>RNA-mediated interference</subject><subject>Science & Technology</subject><subject>Transcription</subject><subject>Transcriptome</subject><subject>Virus Replication</subject><subject>Viruses</subject><issn>1661-6596</issn><issn>1422-0067</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNqNks2LEzEYxgdR3HX15lkGvKzoaD4mXxdhqe1aGPGyehEJmcybNmU6qcmM0v_e1K5l15O5JOT58fC8H0XxHKO3lCr0zm-2iWCCBCPoQXGOa0IqhLh4mN-c44ozxc-KJyltECKUMPW4OKMUMSoVOy_WN2soGxjXpr8kr6p5SjCM3vSVC7FqvIPyW5OF-aL5Xl7DAOXCbH2_Lz-FburNCKn8AMNqgvKrj1Mql4MDO_owlH4or6DLuoHVfjf6p8UjZ_oEz27vi-LLYn4z-1g1n6-Xs6umskzKsepczmU5VZ2oqUSd48K1nCCkJAHOJVYSmLSIG1PXrMWqI6IjlLVGWAYg6EWxPPp2wWz0LvqtiXsdjNd_PkJcaRNHb3vQqAZqsKodp6x2jLamFZYzMICU6ARkr_dHr93UbqGzuTXR9PdM7yuDX-tV-KkFU3k0Mhtc3hrE8GOCNOqtTxb63gwQpqRJzTCVmNeH3C__QTdhikNulSasllwKJg_UmyNlY0gpgjuFwUgf1kHfXYeMv7hbwAn-O_8MvD4Cv6ANLlkPg4UThhBikiIk0eHgTMv_p2d-NIdNmIVpGOlvvgPPSg</recordid><startdate>20201012</startdate><enddate>20201012</enddate><creator>Runtuwene, Lucky R.</creator><creator>Kawashima, Shuichi</creator><creator>Pijoh, Victor D.</creator><creator>Tuda, Josef S. 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| recordid | cdi_proquest_journals_2548687587 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Aedes - genetics Aedes - virology Aedes aegypti Animals Biochemistry & Molecular Biology Chemistry Chemistry, Multidisciplinary Computational Biology - methods dengue Dengue - virology Dengue fever Dengue Virus - physiology Exocrine glands Gene expression Gene Expression Profiling Genes Genomes Heat shock Heat shock proteins Homology Host-Pathogen Interactions HSP20 Heat-Shock Proteins - genetics Immunocompetence Infections Initiation factor eIF-2α Insect Proteins - genetics Insecticides Invertebrates lethal-essential-for-life Life Sciences & Biomedicine Mosquito Vectors - genetics Mosquito Vectors - virology Mosquitoes Phosphorylation Physical Sciences Pollutants Polyinosinic:polycytidylic acid Replication RNA-mediated interference Science & Technology Transcription Transcriptome Virus Replication Viruses |
| title | The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T02%3A13%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Lethal(2)-Essential-for-Life%20%5BL(2)EFL%5D%20Gene%20Family%20Modulates%20Dengue%20Virus%20Infection%20in%20Aedes%20aegypti&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Runtuwene,%20Lucky%20R.&rft.date=2020-10-12&rft.volume=21&rft.issue=20&rft.spage=7520&rft.pages=7520-&rft.artnum=7520&rft.issn=1661-6596&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms21207520&rft_dat=%3Cproquest_cross%3E2548687587%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2548687587&rft_id=info:pmid/33053895&rft_doaj_id=oai_doaj_org_article_04e3a194f6354f53bab7c65eae097d7e&rfr_iscdi=true |