Phylogenetic analysis revealed first report of Eleutheronema rhadinum lineage in the coastal waters of Malaysia
Aim: To genetically identify Eleutheronema specimens and to perform phylogenetic analysis of Eleutheronema from the coastal waters of Malaysia using sequence analysis of mitochondrial Cytochrome c oxidase ! (CO!) gene. Methodology: A total 161 samples of Eleutheronema sp. were collected from Malaysi...
Gespeichert in:
| Veröffentlicht in: | Journal of environmental biology 2020-09, Vol.41, p.1424-1431 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1431 |
|---|---|
| container_issue | |
| container_start_page | 1424 |
| container_title | Journal of environmental biology |
| container_volume | 41 |
| creator | Atikah, N W Esa, Y B Rozihan, M Ismail, M F S Kamaruddin, I S |
| description | Aim: To genetically identify Eleutheronema specimens and to perform phylogenetic analysis of Eleutheronema from the coastal waters of Malaysia using sequence analysis of mitochondrial Cytochrome c oxidase ! (CO!) gene. Methodology: A total 161 samples of Eleutheronema sp. were collected from Malaysian coastal water. DNA was extracted from 26 mg caudal tissue and standard procedures were followed for CO! gene amplification and sequencing. The sequences were then proceeded for the analysis of mitochondrial CO! sequence by using MEGA 7. Results: A total of 20 haplotypes were found. Phylogenetic analysis identified two genealogical lineages; E. tetradactylum and E. rhadinum, respectively, harbouring 10 haplotypes each. The genetic distance range was 16%- 17%, thus supported the two lineages as distinct taxa. E. rhadinum lineage has been reported for the first time from Malaysian coastal water. Interpretation: Mitochondrial analysis successfully distinguished two reciprocally monophyletic taxa using CO! dataset. The results also managed to provide first genetic evidence of E. rhadinum existence in Malaysian coastal waters. |
| doi_str_mv | 10.22438/jeb/41j6(SI)/MS_36 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_2548505102</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2548505102</sourcerecordid><originalsourceid>FETCH-proquest_journals_25485051023</originalsourceid><addsrcrecordid>eNqNTk1Lw0AUXETBoP0FXh540UPMZjdpkrNU7KEg1Ht4ti_Nhu1u3bdR-u9dwR_gXIZhPhgh7kr5pFSl22Kij6Iqp-XDdv1YbLa9Xl6ITOmuyqVuukuRSVVXedvI6losmCeZoDvV1F0m_Nt4tv5AjqLZATq0ZzYMgb4ILe1hMIFjkicfIvgBVpbmOFLwjo4IYcS9cfMRrHGEBwLjILmw88gRLXxjpMC_vQ1aTMt4K64GtEyLP74R9y-r9-fX_BT850wc-8nPId3gPp1ua1mXUun_pX4Ax1ZT6A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2548505102</pqid></control><display><type>article</type><title>Phylogenetic analysis revealed first report of Eleutheronema rhadinum lineage in the coastal waters of Malaysia</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Atikah, N W ; Esa, Y B ; Rozihan, M ; Ismail, M F S ; Kamaruddin, I S</creator><creatorcontrib>Atikah, N W ; Esa, Y B ; Rozihan, M ; Ismail, M F S ; Kamaruddin, I S</creatorcontrib><description>Aim: To genetically identify Eleutheronema specimens and to perform phylogenetic analysis of Eleutheronema from the coastal waters of Malaysia using sequence analysis of mitochondrial Cytochrome c oxidase ! (CO!) gene. Methodology: A total 161 samples of Eleutheronema sp. were collected from Malaysian coastal water. DNA was extracted from 26 mg caudal tissue and standard procedures were followed for CO! gene amplification and sequencing. The sequences were then proceeded for the analysis of mitochondrial CO! sequence by using MEGA 7. Results: A total of 20 haplotypes were found. Phylogenetic analysis identified two genealogical lineages; E. tetradactylum and E. rhadinum, respectively, harbouring 10 haplotypes each. The genetic distance range was 16%- 17%, thus supported the two lineages as distinct taxa. E. rhadinum lineage has been reported for the first time from Malaysian coastal water. Interpretation: Mitochondrial analysis successfully distinguished two reciprocally monophyletic taxa using CO! dataset. The results also managed to provide first genetic evidence of E. rhadinum existence in Malaysian coastal waters.</description><identifier>ISSN: 0254-8704</identifier><identifier>EISSN: 2394-0379</identifier><identifier>DOI: 10.22438/jeb/41j6(SI)/MS_36</identifier><language>eng</language><publisher>Lucknow: Triveni Enterprises</publisher><subject>Animal behavior ; Carbon monoxide ; Coastal waters ; Cytochrome ; Cytochrome-c oxidase ; Cytochromes ; Deoxyribonucleic acid ; DNA ; Eleutheronema ; Environmental science ; Fishing ; Gene amplification ; Gene sequencing ; Genetic distance ; Haplotypes ; Laboratories ; Mitochondria ; Mitochondrial DNA ; Morphology ; Phylogenetics ; Phylogeny ; Sequence analysis ; Taxonomy</subject><ispartof>Journal of environmental biology, 2020-09, Vol.41, p.1424-1431</ispartof><rights>Copyright Triveni Enterprises Sep 2020</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Atikah, N W</creatorcontrib><creatorcontrib>Esa, Y B</creatorcontrib><creatorcontrib>Rozihan, M</creatorcontrib><creatorcontrib>Ismail, M F S</creatorcontrib><creatorcontrib>Kamaruddin, I S</creatorcontrib><title>Phylogenetic analysis revealed first report of Eleutheronema rhadinum lineage in the coastal waters of Malaysia</title><title>Journal of environmental biology</title><description>Aim: To genetically identify Eleutheronema specimens and to perform phylogenetic analysis of Eleutheronema from the coastal waters of Malaysia using sequence analysis of mitochondrial Cytochrome c oxidase ! (CO!) gene. Methodology: A total 161 samples of Eleutheronema sp. were collected from Malaysian coastal water. DNA was extracted from 26 mg caudal tissue and standard procedures were followed for CO! gene amplification and sequencing. The sequences were then proceeded for the analysis of mitochondrial CO! sequence by using MEGA 7. Results: A total of 20 haplotypes were found. Phylogenetic analysis identified two genealogical lineages; E. tetradactylum and E. rhadinum, respectively, harbouring 10 haplotypes each. The genetic distance range was 16%- 17%, thus supported the two lineages as distinct taxa. E. rhadinum lineage has been reported for the first time from Malaysian coastal water. Interpretation: Mitochondrial analysis successfully distinguished two reciprocally monophyletic taxa using CO! dataset. The results also managed to provide first genetic evidence of E. rhadinum existence in Malaysian coastal waters.</description><subject>Animal behavior</subject><subject>Carbon monoxide</subject><subject>Coastal waters</subject><subject>Cytochrome</subject><subject>Cytochrome-c oxidase</subject><subject>Cytochromes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Eleutheronema</subject><subject>Environmental science</subject><subject>Fishing</subject><subject>Gene amplification</subject><subject>Gene sequencing</subject><subject>Genetic distance</subject><subject>Haplotypes</subject><subject>Laboratories</subject><subject>Mitochondria</subject><subject>Mitochondrial DNA</subject><subject>Morphology</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Sequence analysis</subject><subject>Taxonomy</subject><issn>0254-8704</issn><issn>2394-0379</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNTk1Lw0AUXETBoP0FXh540UPMZjdpkrNU7KEg1Ht4ti_Nhu1u3bdR-u9dwR_gXIZhPhgh7kr5pFSl22Kij6Iqp-XDdv1YbLa9Xl6ITOmuyqVuukuRSVVXedvI6losmCeZoDvV1F0m_Nt4tv5AjqLZATq0ZzYMgb4ILe1hMIFjkicfIvgBVpbmOFLwjo4IYcS9cfMRrHGEBwLjILmw88gRLXxjpMC_vQ1aTMt4K64GtEyLP74R9y-r9-fX_BT850wc-8nPId3gPp1ua1mXUun_pX4Ax1ZT6A</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Atikah, N W</creator><creator>Esa, Y B</creator><creator>Rozihan, M</creator><creator>Ismail, M F S</creator><creator>Kamaruddin, I S</creator><general>Triveni Enterprises</general><scope>04Q</scope><scope>04W</scope><scope>3V.</scope><scope>7ST</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>SOI</scope></search><sort><creationdate>20200901</creationdate><title>Phylogenetic analysis revealed first report of Eleutheronema rhadinum lineage in the coastal waters of Malaysia</title><author>Atikah, N W ; Esa, Y B ; Rozihan, M ; Ismail, M F S ; Kamaruddin, I S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_25485051023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal behavior</topic><topic>Carbon monoxide</topic><topic>Coastal waters</topic><topic>Cytochrome</topic><topic>Cytochrome-c oxidase</topic><topic>Cytochromes</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Eleutheronema</topic><topic>Environmental science</topic><topic>Fishing</topic><topic>Gene amplification</topic><topic>Gene sequencing</topic><topic>Genetic distance</topic><topic>Haplotypes</topic><topic>Laboratories</topic><topic>Mitochondria</topic><topic>Mitochondrial DNA</topic><topic>Morphology</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Sequence analysis</topic><topic>Taxonomy</topic><toplevel>online_resources</toplevel><creatorcontrib>Atikah, N W</creatorcontrib><creatorcontrib>Esa, Y B</creatorcontrib><creatorcontrib>Rozihan, M</creatorcontrib><creatorcontrib>Ismail, M F S</creatorcontrib><creatorcontrib>Kamaruddin, I S</creatorcontrib><collection>India Database</collection><collection>India Database: Science & Technology</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Environment Abstracts</collection><jtitle>Journal of environmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atikah, N W</au><au>Esa, Y B</au><au>Rozihan, M</au><au>Ismail, M F S</au><au>Kamaruddin, I S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic analysis revealed first report of Eleutheronema rhadinum lineage in the coastal waters of Malaysia</atitle><jtitle>Journal of environmental biology</jtitle><date>2020-09-01</date><risdate>2020</risdate><volume>41</volume><spage>1424</spage><epage>1431</epage><pages>1424-1431</pages><issn>0254-8704</issn><eissn>2394-0379</eissn><abstract>Aim: To genetically identify Eleutheronema specimens and to perform phylogenetic analysis of Eleutheronema from the coastal waters of Malaysia using sequence analysis of mitochondrial Cytochrome c oxidase ! (CO!) gene. Methodology: A total 161 samples of Eleutheronema sp. were collected from Malaysian coastal water. DNA was extracted from 26 mg caudal tissue and standard procedures were followed for CO! gene amplification and sequencing. The sequences were then proceeded for the analysis of mitochondrial CO! sequence by using MEGA 7. Results: A total of 20 haplotypes were found. Phylogenetic analysis identified two genealogical lineages; E. tetradactylum and E. rhadinum, respectively, harbouring 10 haplotypes each. The genetic distance range was 16%- 17%, thus supported the two lineages as distinct taxa. E. rhadinum lineage has been reported for the first time from Malaysian coastal water. Interpretation: Mitochondrial analysis successfully distinguished two reciprocally monophyletic taxa using CO! dataset. The results also managed to provide first genetic evidence of E. rhadinum existence in Malaysian coastal waters.</abstract><cop>Lucknow</cop><pub>Triveni Enterprises</pub><doi>10.22438/jeb/41j6(SI)/MS_36</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0254-8704 |
| ispartof | Journal of environmental biology, 2020-09, Vol.41, p.1424-1431 |
| issn | 0254-8704 2394-0379 |
| language | eng |
| recordid | cdi_proquest_journals_2548505102 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animal behavior Carbon monoxide Coastal waters Cytochrome Cytochrome-c oxidase Cytochromes Deoxyribonucleic acid DNA Eleutheronema Environmental science Fishing Gene amplification Gene sequencing Genetic distance Haplotypes Laboratories Mitochondria Mitochondrial DNA Morphology Phylogenetics Phylogeny Sequence analysis Taxonomy |
| title | Phylogenetic analysis revealed first report of Eleutheronema rhadinum lineage in the coastal waters of Malaysia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T10%3A26%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phylogenetic%20analysis%20revealed%20first%20report%20of%20Eleutheronema%20rhadinum%20lineage%20in%20the%20coastal%20waters%20of%20Malaysia&rft.jtitle=Journal%20of%20environmental%20biology&rft.au=Atikah,%20N%20W&rft.date=2020-09-01&rft.volume=41&rft.spage=1424&rft.epage=1431&rft.pages=1424-1431&rft.issn=0254-8704&rft.eissn=2394-0379&rft_id=info:doi/10.22438/jeb/41j6(SI)/MS_36&rft_dat=%3Cproquest%3E2548505102%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2548505102&rft_id=info:pmid/&rfr_iscdi=true |