Astaxanthin production and expression of carotenogenic genes in a hyperproducing mutant of Xanthophyllomyces dendrorhous
Astaxanthin is produced by Xanthophyllomyces dendrorhous as its principle carotenoid but the production in the wild type strain is relatively low. A stable astaxanthin-hyperproducing strain, M34, was obtained in our previous work from N-methyl-N'-nitro-N-nitrosoguanidin mutagenesis. In this stu...
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| Veröffentlicht in: | Maejo international journal of science and technology 2021-05, Vol.15 (2), p.96-110 |
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| description | Astaxanthin is produced by Xanthophyllomyces dendrorhous as its principle carotenoid but the production in the wild type strain is relatively low. A stable astaxanthin-hyperproducing strain, M34, was obtained in our previous work from N-methyl-N'-nitro-N-nitrosoguanidin mutagenesis. In this study the wild type and M34 strains were compared in terms of growth and carotenoid production. The expression levels and nucleotide sequences of carotenogenic genes idi, crtE, crtYB, crtI, crtR and crtS of both strains were studied by realtime polymerase chain reaction and polymerase chain reaction respectively. Gene expression results indicated that both crtE and crtS genes showed significantly higher expressions relative to the wild type throughout the production cycle, the highest being 1.3-fold and 3.8fold respectively. The six carotenogenic genes exhibited a total of 38 nucleotide changes after mutation, including a missense mutation in idi gene (glutamic acid?alanine) and crtI gene (histidine?glutamine), and two missense mutations in crtE (lysine?arginine; serine?aspartic acid) and crtR genes (serine?valine; glutamine?proline). |
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A stable astaxanthin-hyperproducing strain, M34, was obtained in our previous work from N-methyl-N'-nitro-N-nitrosoguanidin mutagenesis. In this study the wild type and M34 strains were compared in terms of growth and carotenoid production. The expression levels and nucleotide sequences of carotenogenic genes idi, crtE, crtYB, crtI, crtR and crtS of both strains were studied by realtime polymerase chain reaction and polymerase chain reaction respectively. Gene expression results indicated that both crtE and crtS genes showed significantly higher expressions relative to the wild type throughout the production cycle, the highest being 1.3-fold and 3.8fold respectively. The six carotenogenic genes exhibited a total of 38 nucleotide changes after mutation, including a missense mutation in idi gene (glutamic acid?alanine) and crtI gene (histidine?glutamine), and two missense mutations in crtE (lysine?arginine; serine?aspartic acid) and crtR genes (serine?valine; glutamine?proline).</description><identifier>ISSN: 1905-7873</identifier><identifier>EISSN: 1905-7873</identifier><language>eng</language><publisher>Chiang Mai: Maejo University</publisher><subject>Alanine ; Aspartic acid ; Astaxanthin ; Biomass ; Biosynthesis ; Carbon ; Carotenoids ; DNA polymerase ; Gene expression ; Genes ; Genetic engineering ; Glucose ; Glutamic acid ; Glutamine ; Histidine ; Lysine ; Metabolism ; Missense mutation ; Mutagenesis ; Mutation ; Nucleotides ; Polymerase chain reaction ; Proline ; Serine ; Transcription factors ; Valine ; Xanthophyllomyces dendrorhous</subject><ispartof>Maejo international journal of science and technology, 2021-05, Vol.15 (2), p.96-110</ispartof><rights>2021. This work is published under https://creativecommons.org/licenses/by-nc/4.0 (the “License”). 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A stable astaxanthin-hyperproducing strain, M34, was obtained in our previous work from N-methyl-N'-nitro-N-nitrosoguanidin mutagenesis. In this study the wild type and M34 strains were compared in terms of growth and carotenoid production. The expression levels and nucleotide sequences of carotenogenic genes idi, crtE, crtYB, crtI, crtR and crtS of both strains were studied by realtime polymerase chain reaction and polymerase chain reaction respectively. Gene expression results indicated that both crtE and crtS genes showed significantly higher expressions relative to the wild type throughout the production cycle, the highest being 1.3-fold and 3.8fold respectively. The six carotenogenic genes exhibited a total of 38 nucleotide changes after mutation, including a missense mutation in idi gene (glutamic acid?alanine) and crtI gene (histidine?glutamine), and two missense mutations in crtE (lysine?arginine; serine?aspartic acid) and crtR genes (serine?valine; glutamine?proline).</description><subject>Alanine</subject><subject>Aspartic acid</subject><subject>Astaxanthin</subject><subject>Biomass</subject><subject>Biosynthesis</subject><subject>Carbon</subject><subject>Carotenoids</subject><subject>DNA polymerase</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Glucose</subject><subject>Glutamic acid</subject><subject>Glutamine</subject><subject>Histidine</subject><subject>Lysine</subject><subject>Metabolism</subject><subject>Missense mutation</subject><subject>Mutagenesis</subject><subject>Mutation</subject><subject>Nucleotides</subject><subject>Polymerase chain reaction</subject><subject>Proline</subject><subject>Serine</subject><subject>Transcription factors</subject><subject>Valine</subject><subject>Xanthophyllomyces dendrorhous</subject><issn>1905-7873</issn><issn>1905-7873</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpNjslqwzAQQEVpoSHNPwh6NshaYukYQjcI9JJDbkFr7OBIriSD_feVSQ-dwyww7808gFUtEKsa3pDHf_0z2KR0RSUI5oiTFZh2KctJ-tx2Hg4xmFHnLngovYF2GqJNaRmDg1rGkK0PF-s7DUu2CRZGwnYebLyjnb_A25iLbiFOizYM7dz34Tbrsm-sNzHENozpBTw52Se7-atrcHx_O-4_q8P3x9d-d6iGuia5whJvlUUYM0yF0poJ1yhFmeKs0UoipV2tjMWYOkQNEdwR7rhGiAuEESNr8HrXlgd_Rpvy-RrG6MvFM2a02TIiRE1-AYP_XXI</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Yuan, Khaw Shin</creator><creator>Sim, Ang 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production and expression of carotenogenic genes in a hyperproducing mutant of Xanthophyllomyces dendrorhous</title><author>Yuan, Khaw Shin ; Sim, Ang Fong ; Ling, Few Ling ; Cun, See Too Wei ; Ai Lan, Chew</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p113t-2a26be0225249bcc59f7bb45b857cba0bcf1bde224f04d398f38f8c008902053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alanine</topic><topic>Aspartic acid</topic><topic>Astaxanthin</topic><topic>Biomass</topic><topic>Biosynthesis</topic><topic>Carbon</topic><topic>Carotenoids</topic><topic>DNA polymerase</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Glucose</topic><topic>Glutamic acid</topic><topic>Glutamine</topic><topic>Histidine</topic><topic>Lysine</topic><topic>Metabolism</topic><topic>Missense mutation</topic><topic>Mutagenesis</topic><topic>Mutation</topic><topic>Nucleotides</topic><topic>Polymerase chain reaction</topic><topic>Proline</topic><topic>Serine</topic><topic>Transcription factors</topic><topic>Valine</topic><topic>Xanthophyllomyces dendrorhous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Khaw Shin</creatorcontrib><creatorcontrib>Sim, Ang Fong</creatorcontrib><creatorcontrib>Ling, Few Ling</creatorcontrib><creatorcontrib>Cun, See Too Wei</creatorcontrib><creatorcontrib>Ai Lan, Chew</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials 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international journal of science and technology</jtitle><date>2021-05-01</date><risdate>2021</risdate><volume>15</volume><issue>2</issue><spage>96</spage><epage>110</epage><pages>96-110</pages><issn>1905-7873</issn><eissn>1905-7873</eissn><abstract>Astaxanthin is produced by Xanthophyllomyces dendrorhous as its principle carotenoid but the production in the wild type strain is relatively low. A stable astaxanthin-hyperproducing strain, M34, was obtained in our previous work from N-methyl-N'-nitro-N-nitrosoguanidin mutagenesis. In this study the wild type and M34 strains were compared in terms of growth and carotenoid production. The expression levels and nucleotide sequences of carotenogenic genes idi, crtE, crtYB, crtI, crtR and crtS of both strains were studied by realtime polymerase chain reaction and polymerase chain reaction respectively. Gene expression results indicated that both crtE and crtS genes showed significantly higher expressions relative to the wild type throughout the production cycle, the highest being 1.3-fold and 3.8fold respectively. The six carotenogenic genes exhibited a total of 38 nucleotide changes after mutation, including a missense mutation in idi gene (glutamic acid?alanine) and crtI gene (histidine?glutamine), and two missense mutations in crtE (lysine?arginine; serine?aspartic acid) and crtR genes (serine?valine; glutamine?proline).</abstract><cop>Chiang Mai</cop><pub>Maejo University</pub><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alanine Aspartic acid Astaxanthin Biomass Biosynthesis Carbon Carotenoids DNA polymerase Gene expression Genes Genetic engineering Glucose Glutamic acid Glutamine Histidine Lysine Metabolism Missense mutation Mutagenesis Mutation Nucleotides Polymerase chain reaction Proline Serine Transcription factors Valine Xanthophyllomyces dendrorhous |
| title | Astaxanthin production and expression of carotenogenic genes in a hyperproducing mutant of Xanthophyllomyces dendrorhous |
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