MITOCHONDRIAL FUNCTION AND INSULIN SIGNALLING ARE LOWERED BY SUSTAINED ACYLATED GHRELIN TREATMENT IN RAT ADIPOSE TISSUE
Acylated ghrelin (AG), a gastric orexigenic hormone which plays a relevant role in the regulation of intermediate metabolism, has shown tissue specific effects. In skeletal muscle, both in vitro and in healthy and diseased rodent models experiments show that AG enhances mitochondrial function and in...
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| Veröffentlicht in: | Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2021-08, Vol.87-88, p.111315, Article 111315 |
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| creator | Cappellari, G. Gortan Semolic, A. Caporale, R. Kharrat, F. Zanetti, M. Barazzoni, R. |
| description | Acylated ghrelin (AG), a gastric orexigenic hormone which plays a relevant role in the regulation of intermediate metabolism, has shown tissue specific effects. In skeletal muscle, both in vitro and in healthy and diseased rodent models experiments show that AG enhances mitochondrial function and insulin signalling, with variable effect in reducing tissue inflammation and redox balance. In the liver, AG improves redox state and gluconeogenesis, limits fat accumulation and reduces insulin signalling with no changes in mitochondrial function. AG effects on adipose tissue metabolism are currently largely undefined.
We investigated the impact of four days AG s.c. administration in 12-week-old male healthy Wistar rats (AGT; n=8; twice-a-day 200 ng s.c. non orexigenic dose) compared to vehicle (Con; n=8) on retroperitoneal adipose tissue (AT) mitochondrial enzyme activities (citrate synthase and cytochrome c oxidase), oxidized/total glutathione, cytokine levels (xMAP) and insulin sensitivity in terms of AKT and GSK activating phosphorilation (western blot).
No differences were observed among between groups in cumulative food intake or body weight. AGT had lower (p |
| doi_str_mv | 10.1016/j.nut.2021.111315 |
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We investigated the impact of four days AG s.c. administration in 12-week-old male healthy Wistar rats (AGT; n=8; twice-a-day 200 ng s.c. non orexigenic dose) compared to vehicle (Con; n=8) on retroperitoneal adipose tissue (AT) mitochondrial enzyme activities (citrate synthase and cytochrome c oxidase), oxidized/total glutathione, cytokine levels (xMAP) and insulin sensitivity in terms of AKT and GSK activating phosphorilation (western blot).
No differences were observed among between groups in cumulative food intake or body weight. AGT had lower (p<0.05) mitochondrial enzyme activities compared to Con, with similar (p=NS) inflammatory cytokine profile and redox state. Insulin signalling activation was lower in AGT at AKT level (P<0.05), with similar trend for GSK (p=0.08).
In rat adipose tissue, sustained acylated ghrelin adiministration lowers mitochondrial function and insulin sensitivity. These findings are consistent with reports suggesting a potential adaptative role for AG during starvation, as decreased insulin sensitivity and mitochondrial function both lower energy storage by decreasing lipogenesis in adipose tissue.</description><identifier>ISSN: 0899-9007</identifier><identifier>EISSN: 1873-1244</identifier><identifier>DOI: 10.1016/j.nut.2021.111315</identifier><language>eng</language><publisher>Kidlington: Elsevier Inc</publisher><subject>Adipose tissue ; AKT protein ; Animal models ; Animal tissues ; Body fat ; Body weight ; Citrate synthase ; Cytochrome-c oxidase ; Cytochromes ; Cytokines ; Energy storage ; Enzymatic activity ; Enzymes ; Food intake ; Ghrelin ; Gluconeogenesis ; Glutathione ; Inflammation ; Insulin ; Lipogenesis ; Metabolism ; Mitochondria ; Muscles ; Redox properties ; Sensitivity ; Signaling ; Skeletal muscle ; Starvation</subject><ispartof>Nutrition (Burbank, Los Angeles County, Calif.), 2021-08, Vol.87-88, p.111315, Article 111315</ispartof><rights>2021</rights><rights>Copyright Elsevier Limited Aug 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0899900721001775$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Cappellari, G. Gortan</creatorcontrib><creatorcontrib>Semolic, A.</creatorcontrib><creatorcontrib>Caporale, R.</creatorcontrib><creatorcontrib>Kharrat, F.</creatorcontrib><creatorcontrib>Zanetti, M.</creatorcontrib><creatorcontrib>Barazzoni, R.</creatorcontrib><title>MITOCHONDRIAL FUNCTION AND INSULIN SIGNALLING ARE LOWERED BY SUSTAINED ACYLATED GHRELIN TREATMENT IN RAT ADIPOSE TISSUE</title><title>Nutrition (Burbank, Los Angeles County, Calif.)</title><description>Acylated ghrelin (AG), a gastric orexigenic hormone which plays a relevant role in the regulation of intermediate metabolism, has shown tissue specific effects. In skeletal muscle, both in vitro and in healthy and diseased rodent models experiments show that AG enhances mitochondrial function and insulin signalling, with variable effect in reducing tissue inflammation and redox balance. In the liver, AG improves redox state and gluconeogenesis, limits fat accumulation and reduces insulin signalling with no changes in mitochondrial function. AG effects on adipose tissue metabolism are currently largely undefined.
We investigated the impact of four days AG s.c. administration in 12-week-old male healthy Wistar rats (AGT; n=8; twice-a-day 200 ng s.c. non orexigenic dose) compared to vehicle (Con; n=8) on retroperitoneal adipose tissue (AT) mitochondrial enzyme activities (citrate synthase and cytochrome c oxidase), oxidized/total glutathione, cytokine levels (xMAP) and insulin sensitivity in terms of AKT and GSK activating phosphorilation (western blot).
No differences were observed among between groups in cumulative food intake or body weight. AGT had lower (p<0.05) mitochondrial enzyme activities compared to Con, with similar (p=NS) inflammatory cytokine profile and redox state. Insulin signalling activation was lower in AGT at AKT level (P<0.05), with similar trend for GSK (p=0.08).
In rat adipose tissue, sustained acylated ghrelin adiministration lowers mitochondrial function and insulin sensitivity. These findings are consistent with reports suggesting a potential adaptative role for AG during starvation, as decreased insulin sensitivity and mitochondrial function both lower energy storage by decreasing lipogenesis in adipose tissue.</description><subject>Adipose tissue</subject><subject>AKT protein</subject><subject>Animal models</subject><subject>Animal tissues</subject><subject>Body fat</subject><subject>Body weight</subject><subject>Citrate synthase</subject><subject>Cytochrome-c oxidase</subject><subject>Cytochromes</subject><subject>Cytokines</subject><subject>Energy storage</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Food intake</subject><subject>Ghrelin</subject><subject>Gluconeogenesis</subject><subject>Glutathione</subject><subject>Inflammation</subject><subject>Insulin</subject><subject>Lipogenesis</subject><subject>Metabolism</subject><subject>Mitochondria</subject><subject>Muscles</subject><subject>Redox properties</subject><subject>Sensitivity</subject><subject>Signaling</subject><subject>Skeletal muscle</subject><subject>Starvation</subject><issn>0899-9007</issn><issn>1873-1244</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kF1LwzAUhoMoOKc_wLuA150n_VgTvIpd3AI1lTZFdhW2NIUO3bTdFP-9GfPaq_MeeN_z8SB0S2BCgEzvN5PtYT8JISQTQkhEkjM0IjSNAhLG8TkaAWUsYADpJboahg0AEDZlI_T9LHWRLQo1KyXP8VOtMi0LhbmaYamqOpcKV3KueO7VHPNS4Lx4FaWY4cclrupKc6l8w7NlzrUX80UpjiFdCq6fhdJ-DC65xnwmX4pKYC2rqhbX6KJdvQ3u5q-OUf0kdLYI8mIuM54HloRhGiQJi13EWDP1X0bRlFEKYFsK63UKEEd23VK6im3j0tbGDtoGWBpC61i8oozYaIzuTnM_-t3nwQ17s9kd-q1facIkThglUZp6Fzm5bL8bht615qPv3lf9jyFgjnzNxni-5sjXnPj6zMMp4_z5X53rzWA7t7Wu6Xpn96bZdf-kfwF2BHf3</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Cappellari, G. 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Gortan</creatorcontrib><creatorcontrib>Semolic, A.</creatorcontrib><creatorcontrib>Caporale, R.</creatorcontrib><creatorcontrib>Kharrat, F.</creatorcontrib><creatorcontrib>Zanetti, M.</creatorcontrib><creatorcontrib>Barazzoni, R.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>Nursing & Allied Health Database</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>British Nursing Index</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>British Nursing Index (BNI) (1985 to Present)</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>British Nursing Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Nutrition (Burbank, Los Angeles County, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cappellari, G. Gortan</au><au>Semolic, A.</au><au>Caporale, R.</au><au>Kharrat, F.</au><au>Zanetti, M.</au><au>Barazzoni, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MITOCHONDRIAL FUNCTION AND INSULIN SIGNALLING ARE LOWERED BY SUSTAINED ACYLATED GHRELIN TREATMENT IN RAT ADIPOSE TISSUE</atitle><jtitle>Nutrition (Burbank, Los Angeles County, Calif.)</jtitle><date>2021-08</date><risdate>2021</risdate><volume>87-88</volume><spage>111315</spage><pages>111315-</pages><artnum>111315</artnum><issn>0899-9007</issn><eissn>1873-1244</eissn><abstract>Acylated ghrelin (AG), a gastric orexigenic hormone which plays a relevant role in the regulation of intermediate metabolism, has shown tissue specific effects. In skeletal muscle, both in vitro and in healthy and diseased rodent models experiments show that AG enhances mitochondrial function and insulin signalling, with variable effect in reducing tissue inflammation and redox balance. In the liver, AG improves redox state and gluconeogenesis, limits fat accumulation and reduces insulin signalling with no changes in mitochondrial function. AG effects on adipose tissue metabolism are currently largely undefined.
We investigated the impact of four days AG s.c. administration in 12-week-old male healthy Wistar rats (AGT; n=8; twice-a-day 200 ng s.c. non orexigenic dose) compared to vehicle (Con; n=8) on retroperitoneal adipose tissue (AT) mitochondrial enzyme activities (citrate synthase and cytochrome c oxidase), oxidized/total glutathione, cytokine levels (xMAP) and insulin sensitivity in terms of AKT and GSK activating phosphorilation (western blot).
No differences were observed among between groups in cumulative food intake or body weight. AGT had lower (p<0.05) mitochondrial enzyme activities compared to Con, with similar (p=NS) inflammatory cytokine profile and redox state. Insulin signalling activation was lower in AGT at AKT level (P<0.05), with similar trend for GSK (p=0.08).
In rat adipose tissue, sustained acylated ghrelin adiministration lowers mitochondrial function and insulin sensitivity. These findings are consistent with reports suggesting a potential adaptative role for AG during starvation, as decreased insulin sensitivity and mitochondrial function both lower energy storage by decreasing lipogenesis in adipose tissue.</abstract><cop>Kidlington</cop><pub>Elsevier Inc</pub><doi>10.1016/j.nut.2021.111315</doi></addata></record> |
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| issn | 0899-9007 1873-1244 |
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| subjects | Adipose tissue AKT protein Animal models Animal tissues Body fat Body weight Citrate synthase Cytochrome-c oxidase Cytochromes Cytokines Energy storage Enzymatic activity Enzymes Food intake Ghrelin Gluconeogenesis Glutathione Inflammation Insulin Lipogenesis Metabolism Mitochondria Muscles Redox properties Sensitivity Signaling Skeletal muscle Starvation |
| title | MITOCHONDRIAL FUNCTION AND INSULIN SIGNALLING ARE LOWERED BY SUSTAINED ACYLATED GHRELIN TREATMENT IN RAT ADIPOSE TISSUE |
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